Methidiumpropyl-EDTA

Methidiumpropyl-EDTA

Names
IUPAC name 3,8-Diamino-6-[4-[13-carboxy-9,12-bis(carboxymethyl)-1,7-dioxo-2,6,9,12-tetraazatridec-1-yl]phenyl]-5-methylphenanthridinium
Other names MPE
Identifiers
CAS Number	80082-09-3
3D model (JSmol)	Interactive image
ChemSpider	3620842
ECHA InfoCard	100.149.943
EC Number	621-055-2
PubChem CID	4420455
InChI InChI=1S/C34H39N7O8/c1-39-28-16-24(36)8-10-26(28)25-9-7-23(35)15-27(25)33(39)21-3-5-22(6-4-21)34(49)38-12-2-11-37-29(42)17-40(18-30(43)44)13-14-41(19-31(45)46)20-32(47)48/h3-10,15-16,36H,2,11-14,17-20H2,1H3,(H7,35,37,38,42,43,44,45,46,47,48,49)/p+1 Key: XONAOTJPKINJQH-UHFFFAOYSA-O
SMILES O=C(O)CN(CC(=O)O)CCN(CC(=O)O)CC(=O)NCCCNC(=O)C1=CC=C(C=C1)C=2C3=CC(N)=CC=C3C=4C=CC(N)=CC4[N+]2C
Properties
Chemical formula	C34H40N7O8+
Molar mass	674.734 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). Infobox references

Methidiumpropyl-EDTA (MPE) is composed of the DNA intercalator methidium covalently attached to the metal chelator ethylenediaminetetraacetic acid (EDTA) by a short linker. ^[1] MPE nonspecifically binds and cleaves DNA at multiple locations.

See also

• Ethidium bromide

References

1. Tanner, N.Kyle; Cech, Thomas R. (1985). "Self-catalyzed cyclization of the intervening sequence RNA of Tetrahymena:inhibition by methidiumpropyl-EDTA and localization of the major dye binding sites". Nucleic Acids Research. 13 (21): 7759–7779. doi:10.1093/nar/13.21.7759. PMC   322085 . PMID   2415924.