Bahrain sent a delegation to compete at the 2000 Summer Olympics in Sydney, Australia, which were held from 15 September to 1 October 2000. This was the fifth consecutive Summer Olympics the Kingdom took part in. The delegation consisted of four athletes: sprinter Mariam Mohamed Hadi Al Hilli, middle-distance runner Mohamed Saleh Naji Haidara and short-distance swimmers Dawood Yosuf Mohamed Jassim and Fatema Hameed Gerashi. Al Hilli and Gerashi's inclusion in the Bahraini delegation was the first time in history a Gulf Arab nation had sent female athletes to the Olympic Games. All four did not progress beyond the initial heats of their respective competitions. Bahrain's best performance came from Haidara and Jassim who placed seventh in the heats of the men's 800 metres and the men's 100 metres freestyle. Gerashi was disqualified for a false start in the women's 50 metres freestyle and Al Hilli came eighth in her heat in the women's 100 metres.
Equatorial Guinea participated in the 2000 Summer Olympics in Sydney, Australia, which was held from 15 September to 1 October 2000. The country's participation in Sydney marked its fifth appearance in the Summer Olympics since its debut at the 1984 Summer Olympics. The delegation included one middle-distance runner, one short-distance sprinter and two swimmers: José Luis Ebatela Nvo, Mari Paz Mosanga Motanga, Eric Moussambani and Paula Barila Bolopa respectively. All four athletes qualified for the games through wildcard places. Moussambani was selected as the flag bearer for the opening ceremony. The four athletes were unable to advance beyond the first rounds of their respective events, with Moussambani and Bolopa attracting attention for their poor performances, but were applauded by the crowds.
Paula Barila Bolopa, nicknamed 'The Crawler', is a retired Equatoguinean football player and swimmer. She was one of two Equatorial Guinean swimmers to compete at the 2000 Summer Olympics in Sydney, the other being Eric Moussambani.
Sameera Al Bitar, sometimes listed as "Samira" or "Al-Bitar",, is a 2-time Olympic swimmer from Bahrain.
Timothy Antalfy is an Australian Paralympic swimmer. He represented Australia at the 2012 Summer Paralympics in swimming and won a bronze medal.
Ravil Nachaev is an Uzbek former swimmer, who specialized in sprint freestyle and butterfly events. He is a three-time Olympian, and a gold medalist in the 50 m freestyle at the 2002 Asian Games in Busan, South Korea.
Pilin Tachakittiranan is a Thai former swimmer, who specialized in freestyle and butterfly events. She is a two-time Olympian and a multiple-time age-group record holder in all freestyle distances. Regarded as Thailand's top female swimmer, she has won a total of five gold medals at the Southeast Asian Games.
Kim Min-suk is a retired South Korean swimmer, who specialized in sprint freestyle and backstroke events. He is a two-time Olympian, and a double medalist at the 2002 Asian Games in Busan.
Richard Sam Bera is a former Indonesian swimmer, who specialized in sprint and middle-distance freestyle events. He is a three-time Olympian, a bronze medalist at the Asian Games (1990), and a twenty-three times SEA Games gold-medalist since his debut in 1987. He is regarded as Indonesia's most successful swimmer in the sporting history. Up until 2015, Bera held several Indonesian and Southeast Asian Games records in sprint freestyle events from the Southeast Asian Games.
Dieung Anak Manggang is a Malaysian former swimmer, who specialised in long-distance freestyle and in butterfly events. He is a single-time Olympian (2000), and a multiple-time SEA Games medalist.
Huang Chih-yung is a Taiwanese former swimmer, who specialized in sprint freestyle events. He is a two-time Olympian, and a bronze medalist at the 1998 Asian Games.
Harbeth Fu Wing is a Hong Kong former swimmer, who specialized in sprint freestyle events. He represented Hong Kong, China at the 2000 Summer Olympics, and later became a top 16 finalist at the Asian Games.
Vivienne Maria Rignall is a New Zealand former swimmer, who specialised in sprint freestyle events. Rignall represented New Zealand, as a 27-year-old, at the 2000 Summer Olympics, and eventually, at the 2002 Commonwealth Games, where she became a sixth-place finalist in the 50 m freestyle. She also holds a dual residency status to compete internationally for her parents' homeland.
Leah Simone Martindale-Stancil is a Barbadian former competition swimmer who specialized in sprint freestyle and butterfly events. Martindale represented Barbados in two editions of the Olympic Games, where she became the first black female in history to reach an Olympic swimming final in the 50 m freestyle. She also holds three Barbadian records in a sprint freestyle double and in the 50 m butterfly, and twelve All-American honors, while attending the University of Florida.
Siobhan Trichelle Cropper is a 2-time Olympian and swimmer from Trinidad and Tobago, who specialized in sprint freestyle and butterfly events. Cropper represented Trinidad and Tobago in two editions of the Olympic Games, and eventually captured the 100 m butterfly title at the 1998 Central American and Caribbean Games in Maracaibo, Venezuela. She also holds three Trinidadian records in a sprint freestyle and butterfly double, two NCAA championship titles and fourteen All-American honors, while attending Stanford University.
Yekaterina Tochenaya is a Kyrgyzstani former swimmer, who specialized in sprint and middle-distance freestyle events. She holds a dual citizenship status to compete internationally for Kyrgyzstan and Russia, including her rare appearance at the 2000 Summer Olympics.
Elín Sigurðardóttir is an Icelandic former swimmer, who specialized in sprint freestyle events. Sigurdardottir represented Iceland in two editions of the Olympic Games, and also formerly held an Icelandic record in the 50 m freestyle, before it was eventually broken by Ragnheiður Ragnarsdóttir and Sarah Blake Bateman.
Talía Barrios is a Peruvian former swimmer, who specialized in sprint freestyle events. She represented Peru at the 2000 Summer Olympics, and also trained for the Bolles School Swim Club in Fort Lauderdale, Florida under head coaches Larry Shofe and Gregg Troy.
Tânia Anacleto Gregório was a Mozambican former swimmer, who specialized in sprint freestyle events. She represented Mozambique at the 2000 Summer Olympics, and also held numerous national records in a sprint freestyle double.
Hem Raksmey is a Cambodian former swimmer, who specialized in sprint freestyle and breaststroke events. She represented Cambodia in two editions of the Olympic Games, and also held numerous age group records in all strokes. She also shared a sibling tandem together with her brother Hem Kiry, when they both competed at the 2000 Summer Olympics.
Myanmar 
