NECAB2

NECAB2
Identifiers
Aliases	NECAB2 , EFCBP2, N-terminal EF-hand calcium binding protein 2, stip-2
External IDs	OMIM: 618130 MGI: 2152211 HomoloGene: 62200 GeneCards: NECAB2
Gene location (Human) Chr. Chromosome 16 (human) [1] Band 16q23.3 Start 83,968,244 bp [1] End 84,002,776 bp [1]
Gene location (Mouse) Chr. Chromosome 8 (mouse) [2] Band 8|8 E1 Start 120,173,458 bp [2] End 120,199,379 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in nucleus accumbens caudate nucleus amygdala putamen cingulate gyrus Brodmann area 9 hypothalamus prefrontal cortex entorhinal cortex orbitofrontal cortex Top expressed in habenula olfactory tubercle medial geniculate nucleus medial dorsal nucleus subiculum nucleus accumbens globus pallidus superior colliculus superior frontal gyrus dorsomedial hypothalamic nucleus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function metal ion binding protein binding calcium ion binding A2A adenosine receptor binding type 5 metabotropic glutamate receptor binding identical protein binding Cellular component cytoplasm plasma membrane membrane axon dendrite cell projection Biological process positive regulation of adenosine receptor signaling pathway positive regulation of ERK1 and ERK2 cascade positive regulation of glutamate receptor signaling pathway negative regulation of G protein-coupled receptor internalization positive regulation of protein localization to membrane regulation of amyloid precursor protein biosynthetic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 54550 117148 Ensembl ENSG00000103154 ENSMUSG00000031837 UniProt Q7Z6G3 Q91ZP9 RefSeq (mRNA) NM_019065 NM_001329748 NM_001329749 NM_054095 RefSeq (protein) NP_001316677 NP_001316678 NP_061938 NP_473436 Location (UCSC) Chr 16: 83.97 – 84 Mb Chr 8: 120.17 – 120.2 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

N-terminal EF-hand calcium-binding protein 2 is a protein that in humans is encoded by the NECAB2 gene. ^{[5] [6]}

Model organisms

Necab2 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Plasma immunoglobulins	Normal
Haematology	Normal
Micronucleus test	Normal
Heart weight	Normal
Tail epidermis wholemount	Normal
Brain histopathology	Normal
Eye Histopathology	Normal
Salmonella infection	Normal [7]
Citrobacter infection	Normal [8]
All tests and analysis from [9] [10]

Model organisms have been used in the study of NECAB2 function. A conditional knockout mouse line, called Necab2^{tm1a(KOMP)Wtsi [11] [12]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute. ^{[13] [14] [15]}

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. ^{[9] [16]} Twenty five tests were carried out on mutant mice but no significant abnormalities were observed. ^[9]

References

1. GRCh38: Ensembl release 89: ENSG00000103154 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000031837 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Sugita S, Ho A, Sudhof TC (Jun 2002). "NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern". Neuroscience. 112 (1): 51–63. doi:10.1016/S0306-4522(02)00063-5. PMID   12044471. S2CID   18740496.
6. "Entrez Gene: EFCBP2 EF-hand calcium binding protein 2".
7. "Salmonella infection data for Necab2". Wellcome Trust Sanger Institute.
8. "Citrobacter infection data for Necab2". Wellcome Trust Sanger Institute.
9. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x. S2CID   85911512.
10. Mouse Resources Portal, Wellcome Trust Sanger Institute.
11. "International Knockout Mouse Consortium".
12. "Mouse Genome Informatics".
13. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC   3572410 . PMID   21677750.
14. Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID   21677718. S2CID   39281705.
15. Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi: 10.1016/j.cell.2006.12.018 . PMID   17218247. S2CID   18872015.
16. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi: 10.1186/gb-2011-12-6-224 . PMC   3218837 . PMID   21722353.

Further reading

• Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID   8619474.
• Yu W, Andersson B, Worley KC, et al. (1997). "Large-Scale Concatenation cDNA Sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC   139146 . PMID   9110174.
• Bernier G, Vukovich W, Neidhardt L, et al. (2001). "Isolation and characterization of a downstream target of Pax6 in the mammalian retinal primordium". Development. 128 (20): 3987–94. doi:10.1242/dev.128.20.3987. PMID   11641222.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi: 10.1016/j.cell.2006.03.032 . PMID   16713569. S2CID   13709685.