Ossein is the organic extracellular matrix of bone, which is made of 95% collagen. This substance is used in industry for the production of gelatin and bone glue.
In the early 20th century, bones were found to consist of three types of proteins: ossein (collagens), osseomucoid (proteoglycans) and osseoalbuminoid (elastin). [1] Advances in molecular biology rendered these terms obsolete.
When processed industrially, 1,000 kg of bones yield 300 kg of ossein, which can be rapidly degraded and partially denatured by the prolonged action of slightly acidic boiling water, yielding gelatin. [2] The product is specifically known as ossein gelatin in contrast to skin gelatin, which is generated from animal hides. Depending on the method of extraction, there are various types of ossein gelatin (acid ossein gelatin, limed ossein gelatin, etc.). [3]
Another prominent use of ossein is the production of bone glue, whose yield is 16-20% of the mass of dry bone. [2] Bones that are unsuitable for ossein production are carbonized to generate bone char. [2]
Ossein can be isolated by treating bones with hydrochloric acid, which dissolves the inorganic matrix (calcium phosphate and calcium carbonate). [4] The process was discovered no later than 15th century but only really spread in the 18th century, after Glauber's publications. [5] The resulting liquor carrying calcium chloride and phosphoric acid may then be treated with calcium hydroxide to recover dicalcium phosphate for fertilizers or animal feed supplement.
However, the most popular technique of treating the bone meal is steaming or boiling. [6] This process requires no acid but much more energy and may also produce tricalcium phosphate. [7] As an alternative, the deproteinized bone residue left after the removal of ossein can be used to produce bone ash for the manufacture of bone china. [2]
Biopolymers are natural polymers produced by the cells of living organisms. Like other polymers, biopolymers consist of monomeric units that are covalently bonded in chains to form larger molecules. There are three main classes of biopolymers, classified according to the monomers used and the structure of the biopolymer formed: polynucleotides, polypeptides, and polysaccharides. The Polynucleotides, RNA and DNA, are long polymers of nucleotides. Polypeptides include proteins and shorter polymers of amino acids; some major examples include collagen, actin, and fibrin. Polysaccharides are linear or branched chains of sugar carbohydrates; examples include starch, cellulose, and alginate. Other examples of biopolymers include natural rubbers, suberin and lignin, cutin and cutan, melanin, and polyhydroxyalkanoates (PHAs).
A bone is a rigid organ that constitutes part of the skeleton in most vertebrate animals. Bones protect the various other organs of the body, produce red and white blood cells, store minerals, provide structure and support for the body, and enable mobility. Bones come in a variety of shapes and sizes and have complex internal and external structures. They are lightweight yet strong and hard and serve multiple functions.
Collagen is the main structural protein in the extracellular matrix found in the body's various connective tissues. As the main component of connective tissue, it is the most abundant protein in mammals, making up from 25% to 35% of the whole-body protein content. Collagen consists of amino acids bound together to form a triple helix of elongated fibril known as a collagen helix. It is mostly found in connective tissue such as cartilage, bones, tendons, ligaments, and skin. Collagen makes up 30% of the protein found in the human body. Vitamin E improves the production of collagen.
Gelatin or gelatine is a translucent, colorless, flavorless food ingredient, commonly derived from collagen taken from animal body parts. It is brittle when dry and rubbery when moist. It may also be referred to as hydrolyzed collagen, collagen hydrolysate, gelatine hydrolysate, hydrolyzed gelatine, and collagen peptides after it has undergone hydrolysis. It is commonly used as a gelling agent in food, beverages, medications, drug or vitamin capsules, photographic films, papers, and cosmetics.
Gelatin desserts are desserts made with a sweetened and flavoured processed collagen product (gelatin). This kind of dessert was first recorded as jelly by Hannah Glasse in her 18th-century book The Art of Cookery, appearing in a layer of trifle. Jelly is also featured in the best selling cookbooks of English food writers Eliza Acton and Isabella Beeton in the 19th century.
Phosphorus is a chemical element; it has symbol P and atomic number 15. Elemental phosphorus exists in two major forms, white phosphorus and red phosphorus, but because it is highly reactive, phosphorus is never found as a free element on Earth. It has a concentration in the Earth's crust of about one gram per kilogram. In minerals, phosphorus generally occurs as phosphate.
A fertilizer or fertiliser is any material of natural or synthetic origin that is applied to soil or to plant tissues to supply plant nutrients. Fertilizers may be distinct from liming materials or other non-nutrient soil amendments. Many sources of fertilizer exist, both natural and industrially produced. For most modern agricultural practices, fertilization focuses on three main macro nutrients: nitrogen (N), phosphorus (P), and potassium (K) with occasional addition of supplements like rock flour for micronutrients. Farmers apply these fertilizers in a variety of ways: through dry or pelletized or liquid application processes, using large agricultural equipment or hand-tool methods.
Osteoblasts are cells with a single nucleus that synthesize bone. However, in the process of bone formation, osteoblasts function in groups of connected cells. Individual cells cannot make bone. A group of organized osteoblasts together with the bone made by a unit of cells is usually called the osteon.
Advanced meat recovery (AMR) is a slaughterhouse deboning process by which the last traces of skeletal muscle meat are removed from animal bones after the primal cuts have been carved off manually. The machinery used in this process separates meat from bone by scraping, shaving, or pressing the meat from the bone without breaking or grinding the bone. AMR meat typically is used as an ingredient in products requiring further processing, such as hot dogs. Unlike mechanically separated meat, AMR meat is comparable in appearance, texture, and composition to meat trimmings and similar meat products derived by hand.
Mechanically separated meat (MSM), mechanically recovered/reclaimed meat (MRM), or mechanically deboned meat (MDM) is a paste-like meat product produced by forcing pureed or ground beef, pork, mutton, turkey or chicken, under high pressure through a sieve or similar device to separate the bone from the edible meat tissue. It is sometimes called white slime as an analog to meat-additive pink slime and to meat extracted by advanced meat recovery systems, both of which are different processes. The process entails pureeing or grinding the carcass left after the manual removal of meat from the bones and then forcing the slurry through a sieve under pressure. This puree includes bone, bone marrow, skin, nerves, blood vessels, and the scraps of meat remaining on the bones.
Bone ash is a white material produced by the calcination of bones. Typical bone ash consists of about 55.82% calcium oxide, 42.39% phosphorus pentoxide, and 1.79% water. The exact composition of these compounds varies depending upon the type of bones being used, but generally the formula for bone ash is Ca5(OH)(PO4)3. Bone ash usually has a density around 3.10 g/mL and a melting point of 1670 °C (3038 °F). Most bones retain their cellular structure through calcination.
Rendering is a process that converts waste animal tissue into stable, usable materials. Rendering can refer to any processing of animal products into more useful materials, or, more narrowly, to the rendering of whole animal fatty tissue into purified fats like lard or tallow. Rendering can be carried out on an industrial, farm, or kitchen scale. It can also be applied to non-animal products that are rendered down to pulp. The rendering process simultaneously dries the material and separates the fat from the bone and protein, yielding a fat commodity and a protein meal.
Organic fertilizers are fertilizers that are naturally produced. Fertilizers are materials that can be added to soil or plants, in order to provide nutrients and sustain growth. Typical organic fertilizers include all animal waste including meat processing waste, manure, slurry, and guano; plus plant based fertilizers such as compost; and biosolids. Inorganic "organic fertilizers" include minerals and ash. The organic-mess refers to the Principles of Organic Agriculture, which determines whether a fertilizer can be used for commercial organic agriculture, not whether the fertilizer consists of organic compounds.
Bone meal is a mixture of finely and coarsely ground animal bones and slaughter-house waste products. It is used as a dietary supplement to supply calcium and phosphorus to monogastric livestock in the form of hydroxyapatite. As a slow-release organic fertilizer, it supplies phosphorus, calcium, and a small amount of nitrogen to plants.
Animal glue is an adhesive that is created by prolonged boiling of animal connective tissue in a process called rendering. In addition to being used as an adhesive it is used for coating and sizing, in decorative composition ornaments, and as a clarifying agent.
Meat and bone meal (MBM) is a product of the rendering industry. It is typically about 48–52% protein, 33–35% ash, 8–12% fat, and 4–7% water. It is primarily used in the formulation of animal feed to improve the amino acid profile of the feed. Feeding of MBM to cattle is thought to have been responsible for the spread of BSE ; therefore, in most parts of the world, MBM is no longer allowed in feed for ruminant animals. However, it is still used to feed monogastric animals.
The history of fertilizer has largely shaped political, economic, and social circumstances in their traditional uses. Subsequently, there has been a radical reshaping of environmental conditions following the development of chemically synthesized fertilizers.
Octacalcium phosphate (sometimes referred to as OCP) is a form of calcium phosphate with formula Ca8H2(PO4)6·5H2O. OCP may be a precursor to tooth enamel, dentine, and bones. OCP is a precursor of hydroxyapatite (HA), an inorganic biomineral that is important in bone growth. OCP has garnered lots of attention due to its inherent biocompatibility. While OCP exhibits good properties in terms of bone growth, very stringent synthesis requirements make it difficult for mass productions, but nevertheless has shown promise not only in-vitro, but also in in-vivo clinical case studies.
Manure is organic matter that is used as organic fertilizer in agriculture. Most manure consists of animal feces; other sources include compost and green manure. Manures contribute to the fertility of soil by adding organic matter and nutrients, such as nitrogen, that are utilised by bacteria, fungi and other organisms in the soil. Higher organisms then feed on the fungi and bacteria in a chain of life that comprises the soil food web.
The human skeletal system is a complex organ in constant equilibrium with the rest of the body. In addition to support and structure of the body, bone is the major reservoir for many minerals and compounds essential for maintaining a healthy pH balance. The deterioration of the body with age renders the elderly particularly susceptible to and affected by poor bone health. Illnesses like osteoporosis, characterized by weakening of the bone's structural matrix, increases the risk of hip-fractures and other life-changing secondary symptoms. In 2010, over 258,000 people aged 65 and older were admitted to the hospital for hip fractures. Incidence of hip fractures is expected to rise by 12% in America, with a projected 289,000 admissions in the year 2030. Other sources estimate up to 1.5 million Americans will have an osteoporotic-related fracture each year. The cost of treating these people is also enormous, in 1991 Medicare spent an estimated $2.9 billion for treatment and out-patient care of hip fractures, this number can only be expected to rise.