PCK1

PCK1
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	1KHB, 1KHE, 1KHF, 1KHG, 1M51, 1NHX, 2GMV
Identifiers
Aliases	PCK1 , PEPCK-C, PEPEPCKC, phosphoenolpyruvate carboxykinase 1, PCKDC
External IDs	OMIM: 614168 MGI: 97501 HomoloGene: 1944 GeneCards: PCK1
Gene location (Human)
Chr.	Chromosome 20 (human)
End	57,568,121 bp
Gene location (Mouse)
Chr.	Chromosome 2 (mouse)
End	173,001,066 bp
RNA expression pattern
	Top expressed in
	jejunal mucosa; ; right lobe of liver; ; glomerulus; ; metanephric glomerulus; ; kidney tubule; ; kidney; ; renal medulla; ; rectum; ; duodenum; ; pericardium;
	Top expressed in
	brown adipose tissue; ; kidney; ; left lobe of liver; ; white adipose tissue; ; subcutaneous adipose tissue; ; intercostal muscle; ; mammary gland; ; proximal tubule; ; parotid gland; ; left colon;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	nucleotide binding ; manganese ion binding ; GDP binding ; phosphoenolpyruvate carboxykinase activity ; GTP binding ; metal ion binding ; carboxylic acid binding ; purine nucleotide binding ; lyase activity ; carboxy-lyase activity ; magnesium ion binding ; phosphoenolpyruvate carboxykinase (GTP) activity ;
Cellular component	cytoplasm ; cytosol ; extracellular exosome ;
Biological process	glucose homeostasis ; internal protein amino acid acetylation ; lipid metabolism ; response to activity ; positive regulation of transcription from RNA polymerase II promoter in response to acidic pH ; oxaloacetate metabolic process ; response to insulin ; glycerol biosynthetic process from pyruvate ; cellular response to potassium ion starvation ; cellular response to retinoic acid ; response to lipid ; cellular response to hypoxia ; cellular response to fructose stimulus ; response to interleukin-6 ; glucose metabolic process ; ageing ; response to methionine ; cellular response to interleukin-1 ; cellular response to glucagon stimulus ; cellular response to cAMP ; cellular response to insulin stimulus ; cellular response to tumor necrosis factor ; response to lipopolysaccharide ; gluconeogenesis ;
	Sources:Amigo / QuickGO
Orthologs
	5105
	18534
	ENSG00000124253
	ENSMUSG00000027513
	P35558
	Q9Z2V4
	NM_002591
	NM_011044
	NP_002582
	NP_035174
	Wikidata
View/Edit Human	View/Edit Mouse

Last updated October 18, 2022

Phosphoenolpyruvate carboxykinase 1 (soluble), also known as PCK1, is an enzyme which in humans is encoded by the PCK1 gene.^[5]^[6]

Function

This enzyme is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.^[5]

Interactive pathway map

Click on genes, proteins and metabolites below to link to respective articles.^{[§ 1]}

[[File:

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

|alt=Glycolysis and Gluconeogenesis edit]]

Glycolysis and Gluconeogenesis edit

↑ The interactive pathway map can be edited at WikiPathways: "GlycolysisGluconeogenesis_WP534".

Related Research Articles

Phosphoenolpyruvate carboxykinase is an enzyme in the lyase family used in the metabolic pathway of gluconeogenesis. It converts oxaloacetate into phosphoenolpyruvate and carbon dioxide.

Neuronal acetylcholine receptor subunit alpha-4, also known as nAChRα4, is a protein that in humans is encoded by the CHRNA4 gene. The protein encoded by this gene is a subunit of certain nicotinic acetylcholine receptors (nAChR). Alpha4-containing nAChRs appear to play a crucial role in the addictive response to nicotine..

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 is a protein that in humans is encoded by the GNB2 gene.

F-actin-capping protein subunit alpha-1 is a protein that in humans is encoded by the CAPZA1 gene.

Cytosolic phospholipase A2 gamma is an enzyme that in humans is encoded by the PLA2G4C gene.

Acyl-coenzyme A thioesterase 8 is an enzyme that in humans is encoded by the ACOT8 gene.

Folylpolyglutamate synthase, mitochondrial is an enzyme that in humans is encoded by the FPGS gene.

Regulator of G-protein signaling 1 is a protein that in humans is encoded by the RGS1 gene.

Group 10 secretory phospholipase A2 is an enzyme that in humans is encoded by the PLA2G10 gene.

Guanine nucleotide-binding protein G(T) subunit gamma-T1 is a protein that in humans is encoded by the GNGT1 gene.

Procollagen C-endopeptidase enhancer 1 is an enzyme that in humans is encoded by the PCOLCE gene.

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 is an enzyme that in humans is encoded by the B3GAT3 gene.

Carbohydrate sulfotransferase 15 is an enzyme that in humans is encoded by the CHST15 gene. It belongs to the N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase enzyme class.

Arylacetamide deacetylase is an enzyme that in humans is encoded by the AADAC gene.

Rap1 GTPase-GDP dissociation stimulator 1 is an enzyme that in humans is encoded by the RAP1GDS1 gene.

Sialidase-2 is an enzyme that in humans is encoded by the NEU2 gene.

28S ribosomal protein S21, mitochondrial is a protein that in humans is encoded by the MRPS21 gene.

Phosphatidylinositol 4-kinase type 2-beta is an enzyme that in humans is encoded by the PI4K2B gene.

Amyloid beta A4 precursor protein-binding family B member 1-interacting protein (APBB1IP), also known as APBB1-interacting protein 1 or Rap1-GTP-interacting adapter molecule (RIAM) is a protein that in humans is encoded by the APBB1IP gene.

Phosphoenolpyruvate carboxykinase 2, mitochondrial, is an isozyme of phosphoenolpyruvate carboxykinase that in humans is encoded by the PCK2 gene on chromosome 14. This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.[provided by RefSeq, Apr 2014]

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000124253 - Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000027513 - Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
1 2 "Entrez Gene: PCK1 phosphoenolpyruvate carboxykinase 1 (soluble)".
↑ Pilz AJ, Willer E, Povey S, Abbott CM (October 1992). "The genes coding for phosphoenolpyruvate carboxykinase-1 (PCK1) and neuronal nicotinic acetylcholine receptor alpha 4 subunit (CHRNA4) map to human chromosome 20, extending the known region of homology with mouse chromosome 2". Ann. Hum. Genet. 56 (Pt 4): 289–93. doi:10.1111/j.1469-1809.1992.tb01155.x. PMID 1492743. S2CID 23586336.

Beale EG, Hammer RE, Antoine B, Forest C (2004). "Disregulated glyceroneogenesis: PCK1 as a candidate diabetes and obesity gene". Trends Endocrinol. Metab. 15 (3): 129–35. doi:10.1016/j.tem.2004.02.006. PMID 15046742. S2CID 9194909.
Pilz AJ, Willer E, Povey S, Abbott CM (1993). "The genes coding for phosphoenolpyruvate carboxykinase-1 (PCK1) and neuronal nicotinic acetylcholine receptor alpha 4 subunit (CHRNA4) map to human chromosome 20, extending the known region of homology with mouse chromosome 2". Ann. Hum. Genet. 56 (Pt 4): 289–93. doi:10.1111/j.1469-1809.1992.tb01155.x. PMID 1492743. S2CID 23586336.
Giralt M, Park EA, Gurney AL, et al. (1991). "Identification of a thyroid hormone response element in the phosphoenolpyruvate carboxykinase (GTP) gene. Evidence for synergistic interaction between thyroid hormone and cAMP cis-regulatory elements". J. Biol. Chem. 266 (32): 21991–6. doi: 10.1016/S0021-9258(18)54735-9 . PMID 1657985.
Beale EG, Chrapkiewicz NB, Scoble HA, et al. (1985). "Rat hepatic cytosolic phosphoenolpyruvate carboxykinase (GTP). Structures of the protein, messenger RNA, and gene". J. Biol. Chem. 260 (19): 10748–60. doi: 10.1016/S0021-9258(19)85145-1 . PMID 2993287.
Ting CN, Burgess DL, Chamberlain JS, et al. (1993). "Phosphoenolpyruvate carboxykinase (GTP): characterization of the human PCK1 gene and localization distal to MODY on chromosome 20" (PDF). Genomics. 16 (3): 698–706. doi:10.1006/geno.1993.1250. hdl: 2027.42/30779 . PMID 8325643.
Yu H, Thun R, Chandrasekharappa S, et al. (1993). "Human PCK1 encoding phosphoenolpyruvate carboxykinase is located on chromosome 20q13.2" (PDF). Genomics. 15 (1): 219–21. doi:10.1006/geno.1993.1040. hdl: 2027.42/31063 . PMID 8432541.
Stoffel M, Xiang KS, Espinosa R, et al. (1993). "cDNA sequence and localization of polymorphic human cytosolic phosphoenolpyruvate carboxykinase gene (PCK1) to chromosome 20, band q13.31: PCK1 is not tightly linked to maturity-onset diabetes of the young". Hum. Mol. Genet. 2 (1): 1–4. doi:10.1093/hmg/2.1.1. PMID 8490617.
O'Brien RM, Printz RL, Halmi N, et al. (1996). "Structural and functional analysis of the human phosphoenolpyruvate carboxykinase gene promoter". Biochim. Biophys. Acta. 1264 (3): 284–8. doi:10.1016/0167-4781(95)00194-8. PMID 8547315.
Parissi V, Calmels C, De Soultrait VR, et al. (2001). "Functional interactions of human immunodeficiency virus type 1 integrase with human and yeast HSP60". J. Virol. 75 (23): 11344–53. doi:10.1128/JVI.75.23.11344-11353.2001. PMC 114720 . PMID 11689615.
Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20". Nature. 414 (6866): 865–71. Bibcode:2001Natur.414..865D. doi: 10.1038/414865a . PMID 11780052.
Dunten P, Belunis C, Crowther R, et al. (2002). "Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site". J. Mol. Biol. 316 (2): 257–64. doi:10.1006/jmbi.2001.5364. PMID 11851336.
Jurado LA, Song S, Roesler WJ, Park EA (2002). "Conserved amino acids within CCAAT enhancer-binding proteins (C/EBP(alpha) and beta) regulate phosphoenolpyruvate carboxykinase (PEPCK) gene expression". J. Biol. Chem. 277 (31): 27606–12. doi: 10.1074/jbc.M201429200 . PMID 11997389.
Wilson HL, McFie PJ, Roesler WJ (2003). "Different transcription factor binding arrays modulate the cAMP responsivity of the phosphoenolpyruvate carboxykinase gene promoter". J. Biol. Chem. 277 (46): 43895–902. doi: 10.1074/jbc.M203169200 . PMID 12237288.
Borgius LJ, Steffensen KR, Gustafsson JA, Treuter E (2003). "Glucocorticoid signaling is perturbed by the atypical orphan receptor and corepressor SHP". J. Biol. Chem. 277 (51): 49761–6. doi: 10.1074/jbc.M205641200 . PMID 12324453.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC 139241 . PMID 12477932.
Chakravarty K, Wu SY, Chiang CM, et al. (2004). "SREBP-1c and Sp1 interact to regulate transcription of the gene for phosphoenolpyruvate carboxykinase (GTP) in the liver". J. Biol. Chem. 279 (15): 15385–95. doi: 10.1074/jbc.M309905200 . PMID 14744869.
Cao H, van der Veer E, Ban MR, et al. (2004). "Promoter polymorphism in PCK1 (phosphoenolpyruvate carboxykinase gene) associated with type 2 diabetes mellitus". J. Clin. Endocrinol. Metab. 89 (2): 898–903. doi: 10.1210/jc.2003-031361 . PMID 14764811.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928 . PMID 15489334.
Hajarnis S, Schroeder JM, Curthoys NP (2005). "3'-Untranslated region of phosphoenolpyruvate carboxykinase mRNA contains multiple instability elements that bind AUF1". J. Biol. Chem. 280 (31): 28272–80. doi: 10.1074/jbc.M501204200 . PMID 15951444.

This article on a gene on human chromosome 20 is a stub. You can help Wikipedia by expanding it.

This page is based on this Wikipedia article
Text is available under the CC BY-SA 4.0 license; additional terms may apply.
Images, videos and audio are available under their respective licenses.

[WikiPathways-7] The interactive pathway map can be edited at WikiPathways: "GlycolysisGluconeogenesis_WP534".

[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000124253 - Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000027513 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-5] 1 2 "Entrez Gene: PCK1 phosphoenolpyruvate carboxykinase 1 (soluble)".

[pmid1492743-6] Pilz AJ, Willer E, Povey S, Abbott CM (October 1992). "The genes coding for phosphoenolpyruvate carboxykinase-1 (PCK1) and neuronal nicotinic acetylcholine receptor alpha 4 subunit (CHRNA4) map to human chromosome 20, extending the known region of homology with mouse chromosome 2". Ann. Hum. Genet. 56 (Pt 4): 289–93. doi:10.1111/j.1469-1809.1992.tb01155.x. PMID 1492743. S2CID 23586336.

[1]

[2]

[3]

[4]

[5]

[6]

[§ 1]

PCK1

Contents

Function

Interactive pathway map

See also

Related Research Articles

References

Further reading