PDCD6IP

PDCD6IP
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2OEV, 2OEW, 2OEX, 2OJQ, 2R02, 2R03, 2R05, 2XS1, 2XS8, 2ZNE, 3C3O, 3C3Q, 3C3R, 3E1R, 3WUV, 4JJY
Identifiers
Aliases	PDCD6IP , AIP1, ALIX, DRIP4, HP95, programmed cell death 6 interacting protein
External IDs	OMIM: 608074 MGI: 1333753 HomoloGene: 22614 GeneCards: PDCD6IP
Gene location (Human) Chr. Chromosome 3 (human) [1] Band 3p22.3 Start 33,798,571 bp [1] End 33,869,707 bp [1]
Gene location (Mouse) Chr. Chromosome 9 (mouse) [2] Band 9|9 F3 Start 113,480,812 bp [2] End 113,537,327 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Achilles tendon tibial nerve stromal cell of endometrium rectum gastric mucosa jejunal mucosa monocyte gallbladder appendix right lung Top expressed in lip esophagus thymus epithelium of stomach pontine nuclei left colon dorsal tegmental nucleus medial vestibular nucleus ileum corneal stroma More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein homodimerization activity protein binding protein dimerization activity proteinase activated receptor binding calcium-dependent protein binding Cellular component immunological synapse focal adhesion microtubule organizing center extracellular vesicle endoplasmic reticulum exit site extracellular region myelin sheath cytoskeleton melanosome membrane extracellular exosome cytoplasm cytosol actomyosin bicellular tight junction cell junction Flemming body Biological process cell cycle mitotic metaphase plate congression protein transport positive regulation of exosomal secretion viral life cycle regulation of mitotic spindle assembly ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway positive regulation of extracellular exosome assembly septum digestion after cytokinesis regulation of centrosome duplication nucleus organization multivesicular body assembly regulation of extracellular exosome assembly apoptotic process cell division viral budding via host ESCRT complex viral process actomyosin contractile ring assembly maintenance of epithelial cell apical/basal polarity bicellular tight junction assembly regulation of membrane permeability mitotic cytokinesis viral budding protein homooligomerization transport midbody abscission Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10015 18571 Ensembl ENSG00000170248 ENSMUSG00000032504 UniProt Q8WUM4 Q9WU78 RefSeq (mRNA) NM_001162429 NM_001256192 NM_013374 NM_001164677 NM_001164678 NM_011052 RefSeq (protein) NP_001155901 NP_001243121 NP_037506 NP_001158149 NP_001158150 NP_035182 Location (UCSC) Chr 3: 33.8 – 33.87 Mb Chr 9: 113.48 – 113.54 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Programmed cell death 6-interacting protein also known as ALIX is a protein that in humans is encoded by the PDCD6IP gene. ^{[5] [6]}

This gene encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death. ^[6]

Function

PDCD6IP protein is part of ESCRT pathway. It participates in the membrane scission of the revers topology budding and participates in multivesicular body formation. ^[7] It is also vital at the later stages and for successful completion of cytokinesis. ^[8]

Interactions

PDCD6IP has been shown to interact with PDCD6. ^{[5] [9]} The V domain of PDCD6IP recognises Short linear motif LYPxLxxL and this motif is mimicked by p6 late domain of HIV and related viruses which facilitates viral hijacking of ESCRT pathway and consequential budding of viral particles. ^[10]

References

1. GRCh38: Ensembl release 89: ENSG00000170248 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000032504 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Vito P, Pellegrini L, Guiet C, D'Adamio L (Feb 1999). "Cloning of AIP1, a novel protein that associates with the apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction". J Biol Chem. 274 (3): 1533–40. doi: 10.1074/jbc.274.3.1533 . PMID   9880530.
6. "Entrez Gene: PDCD6IP programmed cell death 6 interacting protein".
7. Vietri M, Radulovic M, Stenmark H (January 2020). "The many functions of ESCRTs". Nature Reviews Molecular Cell Biology. 21 (1): 25–42. doi:10.1038/s41580-019-0177-4. PMID   31705132. S2CID   207939465.
8. Carlton JG, Martin-Serrano J (29 June 2007). "Parallels Between Cytokinesis and Retroviral Budding: A Role for the ESCRT Machinery". Science. 316 (5833): 1908–1912. Bibcode:2007Sci...316.1908C. doi: 10.1126/science.1143422 . PMID   17556548. S2CID   29191843.
9. Satoh H, Shibata Hideki, Nakano Yoshimi, Kitaura Yasuyuki, Maki Masatoshi (Mar 2002). "ALG-2 interacts with the amino-terminal domain of annexin XI in a Ca(2+)-dependent manner". Biochem. Biophys. Res. Commun. United States. 291 (5): 1166–72. doi:10.1006/bbrc.2002.6600. ISSN   0006-291X. PMID   11883939.
10. Votteler J, Sundquist WI (September 2013). "Virus Budding and the ESCRT Pathway". Cell Host & Microbe. 14 (3): 232–241. doi:10.1016/j.chom.2013.08.012. PMC   3819203 . PMID   24034610.

Further reading

• Wood JD, Yuan J, Margolis RL, et al. (1998). "Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins". Mol. Cell. Neurosci. 11 (3): 149–60. doi: 10.1006/mcne.1998.0677 . PMID   9647693. S2CID   20003277.
• Missotten M, Nichols A, Rieger K, Sadoul R (1999). "Alix, a novel mouse protein undergoing calcium-dependent interaction with the apoptosis-linked-gene 2 (ALG-2) protein". Cell Death Differ. 6 (2): 124–9. doi: 10.1038/sj.cdd.4400456 . PMID   10200558.
• Nagase T, Kikuno R, Ishikawa KI, et al. (2000). "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (1): 65–73. doi: 10.1093/dnares/7.1.65 . PMID   10718198.
• Wu Y, Pan S, Che S, et al. (2002). "Overexpression of Hp95 induces G1 phase arrest in confluent HeLa cells". Differentiation. 67 (4–5): 139–53. doi:10.1046/j.1432-0436.2001.670406.x. PMID   11683497.
• Satoh H, Shibata H, Nakano Y, et al. (2002). "ALG-2 interacts with the amino-terminal domain of annexin XI in a Ca(2+)-dependent manner". Biochem. Biophys. Res. Commun. 291 (5): 1166–72. doi:10.1006/bbrc.2002.6600. PMID   11883939.
• Chatellard-Causse C, Blot B, Cristina N, et al. (2002). "Alix (ALG-2-interacting protein X), a protein involved in apoptosis, binds to endophilins and induces cytoplasmic vacuolization". J. Biol. Chem. 277 (32): 29108–15. doi: 10.1074/jbc.M204019200 . PMID   12034747.
• Wu Y, Pan S, Luo W, et al. (2002). "Hp95 promotes anoikis and inhibits tumorigenicity of HeLa cells". Oncogene. 21 (44): 6801–8. doi: 10.1038/sj.onc.1205849 . PMID   12360406.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Vincent O, Rainbow L, Tilburn J, et al. (2003). "YPXL/I is a protein interaction motif recognized by aspergillus PalA and its human homologue, AIP1/Alix". Mol. Cell. Biol. 23 (5): 1647–55. doi:10.1128/MCB.23.5.1647-1655.2003. PMC   151718 . PMID   12588984.
• Schmidt MH, Chen B, Randazzo LM, Bogler O (2004). "SETA/CIN85/Ruk and its binding partner AIP1 associate with diverse cytoskeletal elements, including FAKs, and modulate cell adhesion". J. Cell Sci. 116 (Pt 14): 2845–55. doi:10.1242/jcs.00522. PMID   12771190. S2CID   6863972.
• Katoh K, Shibata H, Suzuki H, et al. (2003). "The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting". J. Biol. Chem. 278 (40): 39104–13. doi: 10.1074/jbc.M301604200 . PMID   12860994.
• Leonard D, Ajuh P, Lamond AI, Legerski RJ (2003). "hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing". Biochem. Biophys. Res. Commun. 308 (4): 793–801. doi:10.1016/S0006-291X(03)01486-4. PMID   12927788.
• Strack B, Calistri A, Craig S, et al. (2003). "AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding". Cell. 114 (6): 689–99. doi: 10.1016/S0092-8674(03)00653-6 . PMID   14505569. S2CID   10733770.
• von Schwedler UK, Stuchell M, Müller B, et al. (2003). "The protein network of HIV budding". Cell. 114 (6): 701–13. doi: 10.1016/S0092-8674(03)00714-1 . PMID   14505570. S2CID   16894972.
• Martin-Serrano J, Yarovoy A, Perez-Caballero D, et al. (2003). "Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins". Proc. Natl. Acad. Sci. U.S.A. 100 (21): 12414–9. Bibcode:2003PNAS..10012414M. doi: 10.1073/pnas.2133846100 . PMC   218772 . PMID   14519844.
• Peck JW, Bowden ET, Burbelo PD (2004). "Structure and function of human Vps20 and Snf7 proteins". Biochem. J. 377 (Pt 3): 693–700. doi:10.1042/BJ20031347. PMC   1223912 . PMID   14583093.
• Katoh K, Shibata H, Hatta K, Maki M (2004). "CHMP4b is a major binding partner of the ALG-2-interacting protein Alix among the three CHMP4 isoforms". Arch. Biochem. Biophys. 421 (1): 159–65. doi:10.1016/j.abb.2003.09.038. PMID   14678797.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Matsuo H, Chevallier J, Mayran N, et al. (2004). "Role of LBPA and Alix in multivesicular liposome formation and endosome organization". Science. 303 (5657): 531–4. Bibcode:2004Sci...303..531M. doi:10.1126/science.1092425. PMID   14739459. S2CID   36353407.

External links

• PDCD6IP human gene location in the UCSC Genome Browser .
• PDCD6IP human gene details in the UCSC Genome Browser .