POLR2J

POLR2J
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 5FLM, 5IY9, 5IYA, 5IYC, 5IYB, 5IY7, 5IY8, 5IYD, 5IY6
Identifiers
Aliases	POLR2J , POLR2J1, RPB11, RPB11A, RPB11m, hRPB14, polymerase (RNA) II subunit J, RNA polymerase II subunit J
External IDs	OMIM: 604150 MGI: 109582 HomoloGene: 4542 GeneCards: POLR2J
Gene location (Human) Chr. Chromosome 7 (human) [1] Band 7q22.1 Start 102,473,128 bp [1] End 102,478,922 bp [1]
Gene location (Mouse) Chr. Chromosome 5 (mouse) [2] Band 5|5 G2 Start 136,145,485 bp [2] End 136,151,801 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in gastrocnemius muscle left ventricle substantia nigra putamen amygdala hypothalamus nucleus accumbens right adrenal gland caudate nucleus pituitary gland Top expressed in yolk sac morula otic placode internal carotid artery external carotid artery medial ganglionic eminence thymus endocardial cushion secondary oocyte lip More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding LRR domain binding protein dimerization activity DNA-directed 5'-3' RNA polymerase activity protein binding RNA polymerase II activity Cellular component nucleoplasm nucleus RNA polymerase II, core complex Biological process mRNA splicing, via spliceosome transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping transcription-coupled nucleotide-excision repair transcription initiation from RNA polymerase II promoter snRNA transcription by RNA polymerase II fibroblast growth factor receptor signaling pathway RNA metabolic process regulation of gene silencing by miRNA transcription by RNA polymerase II transcription, DNA-templated somatic stem cell population maintenance positive regulation of viral transcription Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5439 20022 Ensembl ENSG00000005075 ENSMUSG00000039771 UniProt P52435 O08740 RefSeq (mRNA) NM_006234 NM_001371100 NM_001393919 NM_011293 RefSeq (protein) NP_006225 NP_001358029 n/a Location (UCSC) Chr 7: 102.47 – 102.48 Mb Chr 5: 136.15 – 136.15 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

DNA-directed RNA polymerase II subunit RPB11-a is an enzyme that in humans is encoded by the POLR2J gene.

Function

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. ^[5]

Interactions

POLR2J has been shown to interact with:

• Apoptosis antagonizing transcription factor, ^[6]
• POLR2C, ^{[7] [8]} and
• SATB1. ^[9]

References

1. GRCh38: Ensembl release 89: ENSG00000005075 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000039771 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: POLR2J polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa".
6. Fanciulli M, Bruno T, Di Padova M, De Angelis R, Iezzi S, Iacobini C, Floridi A, Passananti C (May 2000). "Identification of a novel partner of RNA polymerase II subunit 11, Che-1, which interacts with and affects the growth suppression function of Rb". FASEB J. 14 (7): 904–12. doi: 10.1096/fasebj.14.7.904 . PMID   10783144. S2CID   43175069.
7. Acker J, de Graaff M, Cheynel I, Khazak V, Kedinger C, Vigneron M (July 1997). "Interactions between the human RNA polymerase II subunits". J. Biol. Chem. 272 (27): 16815–21. doi: 10.1074/jbc.272.27.16815 . PMID   9201987.
8. Corbi N, Di Padova M, De Angelis R, Bruno T, Libri V, Iezzi S, Floridi A, Fanciulli M, Passananti C (October 2002). "The alpha-like RNA polymerase II core subunit 3 (RPB3) is involved in tissue-specific transcription and muscle differentiation via interaction with the myogenic factor myogenin". FASEB J. 16 (12): 1639–41. doi: 10.1096/fj.02-0123fje . PMID   12207009. S2CID   30243193.
9. Durrin LK, Krontiris TG (June 2002). "The thymocyte-specific MAR binding protein, SATB1, interacts in vitro with a novel variant of DNA-directed RNA polymerase II, subunit 11". Genomics. 79 (6): 809–17. doi:10.1006/geno.2002.6772. PMID   12036295.

Further reading

• Jeang KT (1998). "Tat, Tat-associated kinase, and transcription". J. Biomed. Sci. 5 (1): 24–7. doi:10.1007/BF02253352. PMID   9570510.
• Yankulov K, Bentley D (1998). "Transcriptional control: Tat cofactors and transcriptional elongation". Curr. Biol. 8 (13): R447–9. Bibcode:1998CBio....8.R447Y. doi: 10.1016/S0960-9822(98)70289-1 . PMID   9651670. S2CID   15480646.
• Romano G, Kasten M, De Falco G, Micheli P, Khalili K, Giordano A (2000). "Regulatory functions of Cdk9 and of cyclin T1 in HIV tat transactivation pathway gene expression". J. Cell. Biochem. 75 (3): 357–68. doi:10.1002/(SICI)1097-4644(19991201)75:3<357::AID-JCB1>3.0.CO;2-K. PMID   10536359. S2CID   43685090.
• Marcello A, Zoppé M, Giacca M (2002). "Multiple modes of transcriptional regulation by the HIV-1 Tat transactivator". IUBMB Life. 51 (3): 175–81. doi: 10.1080/152165401753544241 . PMID   11547919. S2CID   10931640.
• Stevens M, De Clercq E, Balzarini J (2007). "The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention". Med Res Rev. 26 (5): 595–625. doi:10.1002/med.20081. PMC   7168390 . PMID   16838299.
• Harrich D, McMillan N, Munoz L, Apolloni A, Meredith L (2007). "Will diverse Tat interactions lead to novel antiretroviral drug targets?". Current Drug Targets. 7 (12): 1595–606. doi:10.2174/138945006779025338. PMID   17168834.
• Kato H, Sumimoto H, Pognonec P, Chen CH, Rosen CA, Roeder RG (1992). "HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors". Genes Dev. 6 (4): 655–66. doi: 10.1101/gad.6.4.655 . PMID   1559613.
• Southgate C, Zapp ML, Green MR (1990). "Activation of transcription by HIV-1 Tat protein tethered to nascent RNA through another protein". Nature. 345 (6276): 640–2. Bibcode:1990Natur.345..640S. doi:10.1038/345640a0. PMID   2190099. S2CID   4233742.
• Wu-Baer F, Sigman D, Gaynor RB (1995). "Specific binding of RNA polymerase II to the human immunodeficiency virus trans-activating region RNA is regulated by cellular cofactors and Tat". Proc. Natl. Acad. Sci. U.S.A. 92 (16): 7153–7. Bibcode:1995PNAS...92.7153W. doi: 10.1073/pnas.92.16.7153 . PMC   41297 . PMID   7638159.
• Herrmann CH, Rice AP (1995). "Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor". J. Virol. 69 (3): 1612–20. doi:10.1128/JVI.69.3.1612-1620.1995. PMC   188757 . PMID   7853496.
• Keen NJ, Gait MJ, Karn J (1996). "Human immunodeficiency virus type-1 Tat is an integral component of the activated transcription-elongation complex". Proc. Natl. Acad. Sci. U.S.A. 93 (6): 2505–10. Bibcode:1996PNAS...93.2505K. doi: 10.1073/pnas.93.6.2505 . PMC   39827 . PMID   8637904.
• Yang X, Herrmann CH, Rice AP (1996). "The human immunodeficiency virus Tat proteins specifically associate with TAK in vivo and require the carboxyl-terminal domain of RNA polymerase II for function". J. Virol. 70 (7): 4576–84. doi:10.1128/JVI.70.7.4576-4584.1996. PMC   190394 . PMID   8676484.
• Fanciulli M, Bruno T, Cerboni C, Bonetto F, Iacobini C, Frati L, Piccoli M, Floridi A, Santoni A, Punturieri A (1996). "Cloning of a novel human RNA polymerase II subunit downregulated by doxorubicin: new potential mechanisms of drug related toxicity". FEBS Lett. 384 (1): 48–52. doi:10.1016/0014-5793(96)00277-3. PMID   8797801. S2CID   7809458.
• Agostini I, Navarro JM, Rey F, Bouhamdan M, Spire B, Vigne R, Sire J (1996). "The human immunodeficiency virus type 1 Vpr transactivator: cooperation with promoter-bound activator domains and binding to TFIIB". J. Mol. Biol. 261 (5): 599–606. doi:10.1006/jmbi.1996.0485. PMID   8800208.
• Zhou Q, Sharp PA (1996). "Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat". Science. 274 (5287): 605–10. Bibcode:1996Sci...274..605Z. doi:10.1126/science.274.5287.605. PMID   8849451. S2CID   13266489.
• Okamoto H, Sheline CT, Corden JL, Jones KA, Peterlin BM (1996). "Trans-activation by human immunodeficiency virus Tat protein requires the C-terminal domain of RNA polymerase II". Proc. Natl. Acad. Sci. U.S.A. 93 (21): 11575–9. Bibcode:1996PNAS...9311575O. doi: 10.1073/pnas.93.21.11575 . PMC   38099 . PMID   8876177.
• Chun RF, Jeang KT (1996). "Requirements for RNA polymerase II carboxyl-terminal domain for activated transcription of human retroviruses human T-cell lymphotropic virus I and HIV-1". J. Biol. Chem. 271 (44): 27888–94. doi: 10.1074/jbc.271.44.27888 . PMID   8910388.
• Parada CA, Roeder RG (1996). "Enhanced processivity of RNA polymerase II triggered by Tat-induced phosphorylation of its carboxy-terminal domain". Nature. 384 (6607): 375–8. Bibcode:1996Natur.384..375P. doi:10.1038/384375a0. PMID   8934526. S2CID   4278432.
• García-Martínez LF, Ivanov D, Gaynor RB (1997). "Association of Tat with purified HIV-1 and HIV-2 transcription preinitiation complexes". J. Biol. Chem. 272 (11): 6951–8. doi: 10.1074/jbc.272.11.6951 . PMID   9054383.
• Cujec TP, Cho H, Maldonado E, Meyer J, Reinberg D, Peterlin BM (1997). "The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme". Mol. Cell. Biol. 17 (4): 1817–23. doi:10.1128/mcb.17.4.1817. PMC   232028 . PMID   9121429.