RAB5A

RAB5A
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1N6H, 1N6I, 1N6K, 1N6L, 1N6N, 1N6O, 1N6P, 1N6R, 1R2Q, 1TU3, 1TU4, 3MJH, 4Q9U
Identifiers
Aliases	RAB5A , RAB5, member RAS oncogene family
External IDs	OMIM: 179512; MGI: 105926; HomoloGene: 68142; GeneCards: RAB5A; OMA:RAB5A - orthologs
Gene location (Human) Chr. Chromosome 3 (human) [1] Band 3p24.3 Start 19,947,097 bp [1] End 19,985,175 bp [1]
Gene location (Mouse) Chr. Chromosome 17 (mouse) [2] Band 17 C|17 27.82 cM Start 53,786,262 bp [2] End 53,814,708 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in gingival epithelium endothelial cell lower lobe of lung skin of thigh corpus epididymis pons Pars compacta human penis pars reticulata medulla oblongata Top expressed in esophagus ganglionic eminence hippocampus proper lens primary visual cortex cerebellar cortex right kidney zone of skin superior frontal gyrus neural tube More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding GDP binding GTP binding protein binding GTPase activity guanyl nucleotide binding Cellular component cytoplasm axon terminus endosome cell projection membrane synaptic vesicle melanosome ruffle plasma membrane endocytic vesicle axon neuronal cell body terminal bouton dendrite somatodendritic compartment early endosome phagocytic vesicle actin cytoskeleton membrane raft cytoplasmic side of early endosome membrane extracellular exosome cytoplasmic vesicle phagocytic vesicle membrane cytosol endosome membrane clathrin-coated vesicle membrane early endosome membrane early phagosome postsynaptic early endosome anchored component of synaptic vesicle membrane Biological process regulation of endocytosis regulation of filopodium assembly endocytosis blood coagulation receptor internalization involved in canonical Wnt signaling pathway regulation of autophagosome assembly synaptic vesicle recycling early endosome to late endosome transport transport protein transport positive regulation of exocytosis regulation of synaptic vesicle exocytosis regulation of endosome size viral RNA genome replication phagocytosis phosphatidylinositol biosynthetic process membrane organization post-translational protein modification intracellular protein transport Rab protein signal transduction regulation of long-term neuronal synaptic plasticity amyloid-beta clearance by transcytosis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5868 271457 Ensembl ENSG00000144566 ENSMUSG00000017831 UniProt P20339 Q9CQD1 RefSeq (mRNA) NM_004162 NM_001292048 NM_025887 RefSeq (protein) NP_001278977 NP_004153 NP_080163 Location (UCSC) Chr 3: 19.95 – 19.99 Mb Chr 17: 53.79 – 53.81 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Ras-related protein Rab-5A is a protein that in humans is encoded by the RAB5A gene. ^{[5] [6]}

Function

RAB5A localizes to early endosomes where it is involved in the recruitment of RAB7A and the maturation of these compartments to early endosomes. ^[7] It drives the maturation of endosomes by transporting vacuolar (H+)-ATPases (V-ATPases) from trans-Golgi network to endocytic vesicles. ^[8]

Interactions

RAB5A has been shown to interact with:

• CHML, ^{[9] [10]}
• RABEP1, ^{[11] [12] [13] [14]}
• SDCBP, ^[15] and
• ZFYVE20 ^[16]

References

1. GRCh38: Ensembl release 89: ENSG00000144566 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000017831 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Rousseau-Merck MF, Zahraoui A, Touchot N, Tavitian A, Berger R (Feb 1991). "Chromosome assignment of four RAS-related RAB genes". Human Genetics. 86 (4): 350–4. doi:10.1007/BF00201831. PMID   1999336. S2CID   30730416.
6. "Entrez Gene: RAB5A RAB5A, member RAS oncogene family".
7. Huotari J, Helenius A (Aug 2011). "Endosome maturation". The EMBO Journal. 30 (17): 3481–500. doi:10.1038/emboj.2011.286. PMC   3181477 . PMID   21878991.
8. Zhang C, Li A, Zhang X, Xiao H (Mar 2011). "A novel TIP30 protein complex regulates EGF receptor signaling and endocytic degradation". The Journal of Biological Chemistry. 286 (11): 9373–81. doi: 10.1074/jbc.M110.207720 . PMC   3058969 . PMID   21252234.
9. Anant JS, Desnoyers L, Machius M, Demeler B, Hansen JC, Westover KD, Deisenhofer J, Seabra MC (Sep 1998). "Mechanism of Rab geranylgeranylation: formation of the catalytic ternary complex". Biochemistry. 37 (36): 12559–68. doi:10.1021/bi980881a. PMID   9730828.
10. Cremers FP, Armstrong SA, Seabra MC, Brown MS, Goldstein JL (Jan 1994). "REP-2, a Rab escort protein encoded by the choroideremia-like gene". The Journal of Biological Chemistry. 269 (3): 2111–7. doi: 10.1016/S0021-9258(17)42142-9 . PMID   8294464.
11. Xiao GH, Shoarinejad F, Jin F, Golemis EA, Yeung RS (Mar 1997). "The tuberous sclerosis 2 gene product, tuberin, functions as a Rab5 GTPase activating protein (GAP) in modulating endocytosis". The Journal of Biological Chemistry. 272 (10): 6097–100. doi: 10.1074/jbc.272.10.6097 . hdl: 20.500.12613/9244 . PMID   9045618.
12. Vitale G, Rybin V, Christoforidis S, Thornqvist P, McCaffrey M, Stenmark H, Zerial M (Apr 1998). "Distinct Rab-binding domains mediate the interaction of Rabaptin-5 with GTP-bound Rab4 and Rab5". The EMBO Journal. 17 (7): 1941–51. doi:10.1093/emboj/17.7.1941. PMC   1170540 . PMID   9524117.
13. Stenmark H, Vitale G, Ullrich O, Zerial M (Nov 1995). "Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion". Cell. 83 (3): 423–32. doi: 10.1016/0092-8674(95)90120-5 . PMID   8521472. S2CID   18768939.
14. Valsdottir R, Hashimoto H, Ashman K, Koda T, Storrie B, Nilsson T (Nov 2001). "Identification of rabaptin-5, rabex-5, and GM130 as putative effectors of rab33b, a regulator of retrograde traffic between the Golgi apparatus and ER". FEBS Letters. 508 (2): 201–9. doi: 10.1016/s0014-5793(01)02993-3 . PMID   11718716. S2CID   21545088.
15. Tomoda T, Kim JH, Zhan C, Hatten ME (Mar 2004). "Role of Unc51.1 and its binding partners in CNS axon outgrowth". Genes & Development. 18 (5): 541–58. doi:10.1101/gad.1151204. PMC   374236 . PMID   15014045.
16. Nielsen E, Christoforidis S, Uttenweiler-Joseph S, Miaczynska M, Dewitte F, Wilm M, Hoflack B, Zerial M (Oct 2000). "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain". The Journal of Cell Biology. 151 (3): 601–12. doi:10.1083/jcb.151.3.601. PMC   2185588 . PMID   11062261.

Further reading

• Bucci C, Parton RG, Mather IH, Stunnenberg H, Simons K, Hoflack B, Zerial M (Sep 1992). "The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway". Cell. 70 (5): 715–28. doi: 10.1016/0092-8674(92)90306-W . PMID   1516130.
• Zahraoui A, Touchot N, Chardin P, Tavitian A (Jul 1989). "The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion". The Journal of Biological Chemistry. 264 (21): 12394–401. doi: 10.1016/S0021-9258(18)63872-4 . PMID   2501306.
• Farnsworth CC, Seabra MC, Ericsson LH, Gelb MH, Glomset JA (Dec 1994). "Rab geranylgeranyl transferase catalyzes the geranylgeranylation of adjacent cysteines in the small GTPases Rab1A, Rab3A, and Rab5A". Proceedings of the National Academy of Sciences of the United States of America. 91 (25): 11963–7. Bibcode:1994PNAS...9111963F. doi: 10.1073/pnas.91.25.11963 . PMC   45356 . PMID   7991565.
• Cremers FP, Armstrong SA, Seabra MC, Brown MS, Goldstein JL (Jan 1994). "REP-2, a Rab escort protein encoded by the choroideremia-like gene". The Journal of Biological Chemistry. 269 (3): 2111–7. doi: 10.1016/S0021-9258(17)42142-9 . PMID   8294464.
• Stenmark H, Vitale G, Ullrich O, Zerial M (Nov 1995). "Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion". Cell. 83 (3): 423–32. doi: 10.1016/0092-8674(95)90120-5 . PMID   8521472. S2CID   18768939.
• Vita F, Soranzo MR, Borelli V, Bertoncin P, Zabucchi G (Sep 1996). "Subcellular localization of the small GTPase Rab5a in resting and stimulated human neutrophils". Experimental Cell Research. 227 (2): 367–73. doi:10.1006/excr.1996.0286. PMID   8831575.
• Xiao GH, Shoarinejad F, Jin F, Golemis EA, Yeung RS (Mar 1997). "The tuberous sclerosis 2 gene product, tuberin, functions as a Rab5 GTPase activating protein (GAP) in modulating endocytosis". The Journal of Biological Chemistry. 272 (10): 6097–100. doi: 10.1074/jbc.272.10.6097 . hdl: 20.500.12613/9244 . PMID   9045618.
• Simonsen A, Lippé R, Christoforidis S, Gaullier JM, Brech A, Callaghan J, Toh BH, Murphy C, Zerial M, Stenmark H (Jul 1998). "EEA1 links PI(3)K function to Rab5 regulation of endosome fusion". Nature. 394 (6692): 494–8. Bibcode:1998Natur.394..494S. doi:10.1038/28879. PMID   9697774. S2CID   4305220.
• Anant JS, Desnoyers L, Machius M, Demeler B, Hansen JC, Westover KD, Deisenhofer J, Seabra MC (Sep 1998). "Mechanism of Rab geranylgeranylation: formation of the catalytic ternary complex". Biochemistry. 37 (36): 12559–68. doi:10.1021/bi980881a. PMID   9730828.
• Bucci C, Chiariello M, Lattero D, Maiorano M, Bruni CB (May 1999). "Interaction cloning and characterization of the cDNA encoding the human prenylated rab acceptor (PRA1)". Biochemical and Biophysical Research Communications. 258 (3): 657–62. doi:10.1006/bbrc.1999.0651. PMID   10329441.
• Callaghan J, Nixon S, Bucci C, Toh BH, Stenmark H (Oct 1999). "Direct interaction of EEA1 with Rab5b". European Journal of Biochemistry. 265 (1): 361–6. doi: 10.1046/j.1432-1327.1999.00743.x . PMID   10491193.
• Shisheva A, Chinni SR, DeMarco C (Sep 1999). "General role of GDP dissociation inhibitor 2 in membrane release of Rab proteins: modulations of its functional interactions by in vitro and in vivo structural modifications". Biochemistry. 38 (36): 11711–21. doi:10.1021/bi990200r. PMID   10512627.
• Masuda ES, Luo Y, Young C, Shen M, Rossi AB, Huang BC, Yu S, Bennett MK, Payan DG, Scheller RH (Mar 2000). "Rab37 is a novel mast cell specific GTPase localized to secretory granules". FEBS Letters. 470 (1): 61–4. doi:10.1016/S0014-5793(00)01288-6. PMID   10722846. S2CID   38218555.
• Hoffenberg S, Liu X, Nikolova L, Hall HS, Dai W, Baughn RE, Dickey BF, Barbieri MA, Aballay A, Stahl PD, Knoll BJ (Aug 2000). "A novel membrane-anchored Rab5 interacting protein required for homotypic endosome fusion". The Journal of Biological Chemistry. 275 (32): 24661–9. doi: 10.1074/jbc.M909600199 . PMID   10818110.
• Nielsen E, Christoforidis S, Uttenweiler-Joseph S, Miaczynska M, Dewitte F, Wilm M, Hoflack B, Zerial M (Oct 2000). "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain". The Journal of Cell Biology. 151 (3): 601–12. doi:10.1083/jcb.151.3.601. PMC   2185588 . PMID   11062261.
• Hartley JL, Temple GF, Brasch MA (Nov 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC   310948 . PMID   11076863.
• Lanzetti L, Rybin V, Malabarba MG, Christoforidis S, Scita G, Zerial M, Di Fiore PP (Nov 2000). "The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5". Nature. 408 (6810): 374–7. Bibcode:2000Natur.408..374L. doi:10.1038/35042605. PMID   11099046. S2CID   4405293.
• Wolf RM, Wilkes JJ, Chao MV, Resh MD (Oct 2001). "Tyrosine phosphorylation of p190 RhoGAP by Fyn regulates oligodendrocyte differentiation". Journal of Neurobiology. 49 (1): 62–78. doi:10.1002/neu.1066. PMID   11536198.
• Seachrist JL, Laporte SA, Dale LB, Babwah AV, Caron MG, Anborgh PH, Ferguson SS (Jan 2002). "Rab5 association with the angiotensin II type 1A receptor promotes Rab5 GTP binding and vesicular fusion". The Journal of Biological Chemistry. 277 (1): 679–85. doi: 10.1074/jbc.M109022200 . PMID   11682489.