E3 ubiquitin-protein ligase RAD18 is an enzyme that in humans is encoded by the RAD18 gene. [5] [6] [7] A knockout in a human colorectal cancer cell line, HCT116, has also been created. [8]
E3 ubiquitin-protein ligase RAD18 is an enzyme that in humans is encoded by the RAD18 gene. [5] [6] [7] A knockout in a human colorectal cancer cell line, HCT116, has also been created. [8]
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through interaction with Rad6, which is a ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [7]
RAD18 has been shown to interact with HLTF, [9] UBE2B [5] [6] and UBE2A. [5] [6]
Alexander J. Varshavsky is a Russian-American biochemist and geneticist. He works at the California Institute of Technology (Caltech) as the Morgan Professor of Biology. Varshavsky left Russia in 1977, emigrating to United States.
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.
p21Cip1, also known as cyclin-dependent kinase inhibitor 1 or CDK-interacting protein 1, is a cyclin-dependent kinase inhibitor (CKI) that is capable of inhibiting all cyclin/CDK complexes, though is primarily associated with inhibition of CDK2. p21 represents a major target of p53 activity and thus is associated with linking DNA damage to cell cycle arrest. This protein is encoded by the CDKN1A gene located on chromosome 6 (6p21.2) in humans.
DNA replication licensing factor MCM7 is a protein that in humans is encoded by the MCM7 gene.
DNA damage-binding protein 1 is a protein that in humans is encoded by the DDB1 gene.
Ubiquitin-conjugating enzyme E2 N is a protein that in humans is encoded by the UBE2N gene.
Kinesin-like protein KIF22 is a protein that in humans is encoded by the KIF22 gene.
E3 ubiquitin-protein ligase synoviolin is an enzyme that in humans is encoded by the SYVN1 gene.
Ubiquitin-conjugating enzyme E2 E3 is a protein that in humans is encoded by the UBE2E3 gene.
DNA repair protein REV1 is a protein that in humans is encoded by the REV1 gene.
DNA excision repair protein ERCC-8 is a protein that in humans is encoded by the ERCC8 gene.
Ubiquitin-conjugating enzyme E2 A is a protein that in humans is encoded by the UBE2A gene.
The human gene UBR1 encodes the enzyme ubiquitin-protein ligase E3 component n-recognin 1.
Ubiquitin-conjugating enzyme E2 B is a protein that in humans is encoded by the UBE2B gene.
E3 ubiquitin-protein ligase BRE1A is an enzyme that in humans is encoded by the RNF20 gene.
Structural maintenance of chromosomes protein 5 is a protein encoded by the SMC5 gene in human.
Zinc finger and BTB domain-containing protein 32 is a protein that in humans is encoded by the 1960 bp ZBTB32 gene. The 52 kDa protein is a transcriptional repressor and the gene is expressed in T and B cells upon activation, but also significantly in testis cells. It is a member of the Poxviruses and Zinc-finger (POZ) and Krüppel (POK) family of proteins, and was identified in multiple screens involving either immune cell tumorigenesis or immune cell development.
Myosin-XVIIIb is a protein that in humans is encoded by the MYO18B gene.
E3 ubiquitin-protein ligase RNF125 is an enzyme that in humans is encoded by the RNF125 gene.
Polymerase nu is a protein in humans that is encoded by the POLN gene. It is a family A DNA polymerase, considered to be the least effective of the polymerase enzymes. However, DNA polymerase nu plays an active role in homology repair during cellular responses to crosslinks, fulfilling its role in a complex with helicase.