RNF144A

Last updated
RNF144A
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases RNF144A , RNF144, UBCE7IP4, ring finger protein 144A, hUIP4, UIP4
External IDs MGI: 1344401 HomoloGene: 40982 GeneCards: RNF144A
Orthologs
SpeciesHumanMouse
Entrez

9781

108089

Ensembl

ENSG00000151692

ENSMUSG00000020642

UniProt

P50876

Q925F3

RefSeq (mRNA)

NM_001081977
NM_080563

RefSeq (protein)

NP_001075446
NP_542130

Location (UCSC) Chr 2: 6.92 – 7.07 Mb Chr 12: 26.35 – 26.47 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

RNF144A is an E3 ubiquitin ligase belonging to the RING-between RING (RBR) family of ubiquitin ligases, whose specific members have been shown to function as RING-HECT hybrid E3 ligases. [5] [6] [7] RNF144A is most closely related to RNF144B at the protein level, and the two proteins together comprise a subdomain within the RBR family of proteins. [8] [9] [10] The ubiquitin ligase activity of RNF144A catalyzes ubiquitin linkages at the K6-, K11- and K48- positions of ubiquitin in vitro, and is regulated by self-association through its transmembrane domain. [11] [12]

The biological functions of RNF144A is/are relatively unknown beyond its intrinsic enzymatic activity. Somatic mutations of RNF144A have been catalogued in cancer genetic databases in several primary human tumors, including breast, stomach, lymphoma, glioblastoma, uterine and lung cancers. Other members of the RBR family have been associated with neurological and immunological diseases, most notably parkin, HOIL-1L and HOIP(RNF31). [13] [14] [15] [16]

Current known substrates of RNF144A targeted for degradation are proteins involved in DNA repair, heatshock/chaperone function and signalling, consistent with the predominant association of this protein with cancer, and include (DNA-PKcs), PARP1, HSPA2, BMI1, and RAF1. [17] [18] [19] [20] [21]

See also

Related Research Articles

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<span class="mw-page-title-main">UBE2L6</span> Protein-coding gene in the species Homo sapiens

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<span class="mw-page-title-main">HACE1</span> Protein-coding gene found in humans

HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 is a protein that in humans is encoded by the HACE1 gene.

Linear ubiquitin chain assembly complex (LUBAC) is a multi-protein complex and the only known E3 ubiquitin ligase able to conjugate ubiquitin in a head-to-tail manner to generate linear (M1-linked) polyubiquitin chains. The complex is currently known to be composed of three proteins: heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1), HOIL-1-interacting protein (HOIP), and Shank-associated RH domain-interacting protein (SHARPIN),,. HOIL-1 and HOIP are both E3 ubiquitin ligases, however, the specific linear ubiquitin-ligating activity is enacted by HOIP. Mice deficient in HOIP are embryonically lethal. Two cases of mutated HOIP have been detected in humans. These patients presented with autoinflammation and immunodeficiency,. HOIL-1 is required for LUBAC assembly and stability as demonstrated by embryonic lethality in HOIL-1 deficient mice. Recently, it has been noted, that HOIL-1 is also able to catalyze formation of oxyester bonds between the C-terminus of ubiquitin and serine/threonine of substrate protein in TLR signaling. SHARPIN exhibits a significant sequence similarity to HOIL-1 and is important for LUBAC stability. Spontaneous point mutation in the Sharpin gene in mice leads to development of chronic proliferative dermatitis (cpdm),. Both HOIL-1 and SHARPIN bind to HOIP through their ubiquitin-like (UBL) domain,. LUBAC consisting of either HOIP-HOIL-1 or HOIP-SHARPIN is functional in vitro, however the greatest activity of the complex has been observed in the presence of all three components.

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000151692 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000020642 - Ensembl, May 2017
  3. ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ↑ Wenzel DM, Lissounov A, Brzovic PS, Klevit RE (June 2011). "UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids". Nature. 474 (7349): 105–8. doi:10.1038/nature09966. PMC   3444301 . PMID   21532592.
  6. ↑ "RNF144A ring finger protein 144A [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. National Center for Biotechnology Information, U.S. National Library of Medicine . Retrieved 2019-10-27.
  7. ↑ "RNF144A - E3 ubiquitin-protein ligase RNF144A - Homo sapiens (Human) - RNF144A gene & protein". www.uniprot.org. UniProt . Retrieved 2019-10-27.
  8. ↑ Dove KK, Klevit RE (November 2017). "RING-Between-RING E3 Ligases: Emerging Themes amid the Variations". Journal of Molecular Biology. 429 (22): 3363–3375. doi:10.1016/j.jmb.2017.08.008. PMC   5675740 . PMID   28827147.
  9. ↑ Spratt DE, Walden H, Shaw GS (March 2014). "RBR E3 ubiquitin ligases: new structures, new insights, new questions". The Biochemical Journal. 458 (3): 421–37. doi:10.1042/BJ20140006. PMC   3940038 . PMID   24576094.
  10. ↑ Eisenhaber B, Chumak N, Eisenhaber F, Hauser MT (2007). "The ring between ring fingers (RBR) protein family". Genome Biology. 8 (3): 209. doi: 10.1186/gb-2007-8-3-209 . PMC   1868946 . PMID   17367545.
  11. ↑ Michel MA, Swatek KN, Hospenthal MK, Komander D (October 2017). "Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked Ubiquitin Signaling". Molecular Cell. 68 (1): 233–246.e5. doi:10.1016/j.molcel.2017.08.020. PMC   5640506 . PMID   28943312.
  12. ↑ Ho SR, Lee YJ, Lin WC (September 2015). "Regulation of RNF144A E3 Ubiquitin Ligase Activity by Self-association through Its Transmembrane Domain". The Journal of Biological Chemistry. 290 (38): 23026–38. doi: 10.1074/jbc.M115.645499 . PMC   4645615 . PMID   26216882.
  13. ↑ Pickrell AM, Youle RJ (January 2015). "The roles of PINK1, parkin, and mitochondrial fidelity in Parkinson's disease". Neuron. 85 (2): 257–73. doi:10.1016/j.neuron.2014.12.007. PMC   4764997 . PMID   25611507.
  14. ↑ Oda H, Beck DB, Kuehn HS, Sampaio Moura N, Hoffmann P, Ibarra M, et al. (2019-03-18). "Second Case of HOIP Deficiency Expands Clinical Features and Defines Inflammatory Transcriptome Regulated by LUBAC". Frontiers in Immunology. 10: 479. doi: 10.3389/fimmu.2019.00479 . PMC   6431612 . PMID   30936877.
  15. ↑ Boisson B, Laplantine E, Dobbs K, Cobat A, Tarantino N, Hazen M, et al. (June 2015). "Human HOIP and LUBAC deficiency underlies autoinflammation, immunodeficiency, amylopectinosis, and lymphangiectasia". The Journal of Experimental Medicine. 212 (6): 939–51. doi:10.1084/jem.20141130. PMC   4451137 . PMID   26008899.
  16. ↑ Boisson B, Laplantine E, Prando C, Giliani S, Israelsson E, Xu Z, et al. (December 2012). "Immunodeficiency, autoinflammation and amylopectinosis in humans with inherited HOIL-1 and LUBAC deficiency". Nature Immunology. 13 (12): 1178–86. doi:10.1038/ni.2457. PMC   3514453 . PMID   23104095.
  17. ↑ Ho SR, Mahanic CS, Lee YJ, Lin WC (July 2014). "RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage". Proceedings of the National Academy of Sciences of the United States of America. 111 (26): E2646-55. Bibcode:2014PNAS..111E2646H. doi: 10.1073/pnas.1323107111 . PMC   4084471 . PMID   24979766.
  18. ↑ Zhang Y, Liao XH, Xie HY, Shao ZM, Li DQ (November 2017). "RBR-type E3 ubiquitin ligase RNF144A targets PARP1 for ubiquitin-dependent degradation and regulates PARP inhibitor sensitivity in breast cancer cells". Oncotarget. 8 (55): 94505–94518. doi:10.18632/oncotarget.21784. PMC   5706891 . PMID   29212245.
  19. ↑ Yang YL, Zhang Y, Li DD, Zhang FL, Liu HY, Liao XH, et al. (August 2019). "RNF144A functions as a tumor suppressor in breast cancer through ubiquitin ligase activity-dependent regulation of stability and oncogenic functions of HSPA2". Cell Death and Differentiation. 27 (3): 1105–1118. doi:10.1038/s41418-019-0400-z. PMC   7205990 . PMID   31406303.
  20. ↑ Jin X, Kim LJ, Wu Q, Wallace LC, Prager BC, Sanvoranart T, et al. (November 2017). "Targeting glioma stem cells through combined BMI1 and EZH2 inhibition". Nature Medicine. 23 (11): 1352–1361. doi:10.1038/nm.4415. PMC   5679732 . PMID   29035367.
  21. ↑ Afzali B, Kim S, West E, Nova-Lamperti E, Cheru N, Nagashima H, et al. (2019-09-26). "RNF144A shapes the hierarchy of cytokine signaling to provide protective immunity against influenza". doi: 10.1101/782680 .{{cite journal}}: Cite journal requires |journal= (help)
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