SF3B2

SF3B2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2DO5
Identifiers
Aliases	SF3B2 , Cus1, SAP145, SF3B145, SF3b1, SF3b150, splicing factor 3b subunit 2, CFM
External IDs	OMIM: 605591; MGI: 2441856; HomoloGene: 6678; GeneCards: SF3B2; OMA:SF3B2 - orthologs
Gene location (Human) Chr. Chromosome 11 (human) [1] Band 11q13.1 Start 66,050,729 bp [1] End 66,069,308 bp [1]
Gene location (Mouse) Chr. Chromosome 19 (mouse) [2] Band 19|19 A Start 5,323,960 bp [2] End 5,345,483 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ventricular zone left testis right testis anterior pituitary right uterine tube ganglionic eminence right lobe of thyroid gland left lobe of thyroid gland right hemisphere of cerebellum body of uterus Top expressed in Rostral migratory stream external carotid artery internal carotid artery supraoptic nucleus condyle conjunctival fornix fossa substantia nigra motor neuron primitive streak More reference expression data BioGPS n/a
Gene ontology Molecular function protein binding RNA binding Cellular component catalytic step 2 spliceosome spliceosomal complex U2-type spliceosomal complex U12-type spliceosomal complex precatalytic spliceosome nucleus U2 snRNP nucleoplasm nuclear speck U2-type precatalytic spliceosome Biological process mRNA splicing, via spliceosome mRNA processing viral process RNA splicing Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10992 319322 Ensembl ENSG00000087365 ENSMUSG00000024853 UniProt Q13435 Q3UJB0 RefSeq (mRNA) NM_006842 NM_030109 NM_001362454 RefSeq (protein) NP_006833 NP_084385 NP_001349383 Location (UCSC) Chr 11: 66.05 – 66.07 Mb Chr 19: 5.32 – 5.35 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Splicing factor 3B subunit 2 is a protein that in humans is encoded by the SF3B2 gene. ^{[5] [6]}

Function

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. ^[6]

Interactions

SF3B2 has been shown to interact with SF3B4, ^{[7] [8]} RBM7, ^[9] SF3B1 ^{[10] [11]} and CDC5L. ^[12]

References

1. GRCh38: Ensembl release 89: ENSG00000087365 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000024853 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Gozani O, Feld R, Reed R (Jan 1996). "Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A". Genes & Development. 10 (2): 233–43. doi: 10.1101/gad.10.2.233 . PMID   8566756.
6. "Entrez Gene: SF3B2 splicing factor 3b, subunit 2, 145kDa".
7. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
8. Champion-Arnaud P, Reed R (Aug 1994). "The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site". Genes & Development. 8 (16): 1974–83. doi: 10.1101/gad.8.16.1974 . PMID   7958871.
9. Guo TB, Boros LG, Chan KC, Hikim AP, Hudson AP, Swerdloff RS, Mitchell AP, Salameh WA (2003). "Spermatogenetic expression of RNA-binding motif protein 7, a protein that interacts with splicing factors". Journal of Andrology. 24 (2): 204–14. doi:10.1002/j.1939-4640.2003.tb02664.x. PMID   12634307.
10. Das BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R (Oct 1999). "Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155". Molecular and Cellular Biology. 19 (10): 6796–802. doi:10.1128/mcb.19.10.6796. PMC   84676 . PMID   10490618.
11. Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R (Sep 2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". The EMBO Journal. 21 (18): 4978–88. doi:10.1093/emboj/cdf480. PMC   126279 . PMID   12234937.
12. Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (Dec 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". The EMBO Journal. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC   305846 . PMID   11101529.

Further reading

• Agell N, Aligué R, Alemany V, Castro A, Jaime M, Pujol MJ, Rius E, Serratosa J, Taulés M, Bachs O (1998). "New nuclear functions for calmodulin". Cell Calcium. 23 (2–3): 115–21. doi:10.1016/S0143-4160(98)90109-9. PMID   9601606.
• Champion-Arnaud P, Reed R (Aug 1994). "The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site". Genes & Development. 8 (16): 1974–83. doi: 10.1101/gad.8.16.1974 . PMID   7958871.
• Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M (Sep 1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nature Genetics. 20 (1): 46–50. doi:10.1038/1700. PMID   9731529. S2CID   585778.
• Das BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R (Oct 1999). "Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155". Molecular and Cellular Biology. 19 (10): 6796–802. doi:10.1128/mcb.19.10.6796. PMC   84676 . PMID   10490618.
• Das R, Zhou Z, Reed R (May 2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E". Molecular Cell. 5 (5): 779–87. doi: 10.1016/S1097-2765(00)80318-4 . PMID   10882114.
• Bryant HE, Wadd SE, Lamond AI, Silverstein SJ, Clements JB (May 2001). "Herpes simplex virus IE63 (ICP27) protein interacts with spliceosome-associated protein 145 and inhibits splicing prior to the first catalytic step". Journal of Virology. 75 (9): 4376–85. doi:10.1128/JVI.75.9.4376-4385.2001. PMC   114182 . PMID   11287586.
• Brand M, Moggs JG, Oulad-Abdelghani M, Lejeune F, Dilworth FJ, Stevenin J, Almouzni G, Tora L (Jun 2001). "UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation". The EMBO Journal. 20 (12): 3187–96. doi:10.1093/emboj/20.12.3187. PMC   150203 . PMID   11406595.
• Will CL, Schneider C, MacMillan AM, Katopodis NF, Neubauer G, Wilm M, Lührmann R, Query CC (Aug 2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site". The EMBO Journal. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMC   125580 . PMID   11500380.
• Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ (Apr 2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC   1370266 . PMID   11991638.
• Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R (Sep 2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". The EMBO Journal. 21 (18): 4978–88. doi:10.1093/emboj/cdf480. PMC   126279 . PMID   12234937.
• Warner DR, Roberts EA, Greene RM, Pisano MM (Dec 2003). "Identification of novel Smad binding proteins". Biochemical and Biophysical Research Communications. 312 (4): 1185–90. doi:10.1016/j.bbrc.2003.11.049. PMID   14651998.
• Will CL, Schneider C, Hossbach M, Urlaub H, Rauhut R, Elbashir S, Tuschl T, Lührmann R (Jun 2004). "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome". RNA. 10 (6): 929–41. doi:10.1261/rna.7320604. PMC   1370585 . PMID   15146077.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi: 10.1073/pnas.0404720101 . PMC   514446 . PMID   15302935.
• Lin KT, Lu RM, Tarn WY (Oct 2004). "The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo". Molecular and Cellular Biology. 24 (20): 9176–85. doi:10.1128/MCB.24.20.9176-9185.2004. PMC   517884 . PMID   15456888.
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (Jan 2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID   15635413. S2CID   4344740.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Ardon O, Zimmerman ES, Andersen JL, DeHart JL, Blackett J, Planelles V (Apr 2006). "Induction of G2 arrest and binding to cyclophilin A are independent phenotypes of human immunodeficiency virus type 1 Vpr". Journal of Virology. 80 (8): 3694–700. doi:10.1128/JVI.80.8.3694-3700.2006. PMC   1440437 . PMID   16571786.