CDC5L

CDC5L
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2DIM, 2DIN
Identifiers
Aliases	CDC5L , CDC5, CDC5-LIKE, CEF1, PCDC5RP, dJ319D22.1, cell division cycle 5 like
External IDs	OMIM: 602868 MGI: 1918952 HomoloGene: 13291 GeneCards: CDC5L
Gene location (Human) Chr. Chromosome 6 (human) [1] Band 6p21.1 Start 44,387,706 bp [1] End 44,450,425 bp [1]
Gene location (Mouse) Chr. Chromosome 17 (mouse) [2] Band 17|17 B3 Start 45,702,810 bp [2] End 45,744,663 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sperm tibia internal globus pallidus secondary oocyte amniotic fluid biceps brachii Brodmann area 23 palpebral conjunctiva middle temporal gyrus visceral pleura Top expressed in maxillary prominence primitive streak morula renal corpuscle blastocyst medullary collecting duct medial ganglionic eminence hand facial motor nucleus dermis More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding protein phosphatase 1 binding leucine zipper domain binding WD40-repeat domain binding protein binding transcription corepressor binding protein kinase binding sequence-specific DNA binding DNA-binding transcription factor activity, RNA polymerase II-specific RNA binding RNA polymerase II transcription regulatory region sequence-specific DNA binding DNA-binding transcription activator activity, RNA polymerase II-specific Cellular component cytoplasm nuclear speck catalytic step 2 spliceosome membrane Prp19 complex nucleoplasm protein-DNA complex perinuclear region of cytoplasm DNA replication factor A complex spliceosomal complex nucleus U2-type catalytic step 2 spliceosome Biological process mRNA splicing, via spliceosome cellular response to nerve growth factor stimulus regulation of transcription, DNA-templated mRNA processing cellular response to fibroblast growth factor stimulus transcription, DNA-templated cellular response to interleukin-2 cellular response to prolactin cellular response to DNA damage stimulus cellular response to wortmannin mitotic cell cycle cell cycle DNA repair cell differentiation RNA splicing regulation of transcription by RNA polymerase II transcription by RNA polymerase II positive regulation of transcription by RNA polymerase II Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 988 71702 Ensembl ENSG00000096401 ENSMUSG00000023932 UniProt Q99459 Q6A068 RefSeq (mRNA) NM_001253 NM_152810 RefSeq (protein) NP_001244 NP_690023 Location (UCSC) Chr 6: 44.39 – 44.45 Mb Chr 17: 45.7 – 45.74 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Cell division cycle 5-like protein is a protein that in humans is encoded by the CDC5L gene. ^{[5] [6] [7]}

Function

The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G₂/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G₂/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. ^[7]

Interactions

CDC5L has been shown to interact with:

• ASF/SF2, ^[8]
• BZW1, ^[8]
• CWC15, ^[8]
• DNA-PKcs, ^[8]
• DYNC1H1, ^[8]
• GCN1L1, ^[8]
• HSPA8, ^[8]
• ILF2, ^[8]
• PLRG1, ^{[8] [9] [10] [11]}
• PPM1D, ^[8]
• PPP1CA, ^[8]
• PRPF19, ^[8]
• RBMX ^[8] and
• RPL12, ^[8]
• RPL13, ^[8]
• RPS16, ^[8]
• RPS25, ^[8]
• SF3A1, ^[8]
• SF3B1, ^[8]
• SF3B2, ^[8]
• SF3B4, ^[8]
• SFPQ, ^[8]
• SFRS2, ^[8]
• SNRPA1, ^[8]
• SNRPD3, ^[8]
• SRRM1, ^[8]
• Small nuclear ribonucleoprotein D1, ^[8]
• Small nuclear ribonucleoprotein D2, ^[8]
• Small nuclear ribonucleoprotein polypeptide A, ^[8]
• TOP2A, ^[8] and
• TTF2. ^[12]

See also

• G2/M checkpoint

References

1. GRCh38: Ensembl release 89: ENSG00000096401 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000023932 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Groenen PM, Vanderlinden G, Devriendt K, Fryns JP, Van de Ven WJ (Apr 1998). "Rearrangement of the human CDC5L gene by a t(6;19)(p21;q13.1) in a patient with multicystic renal dysplasia". Genomics. 49 (2): 218–29. doi:10.1006/geno.1998.5254. PMID   9598309.
6. Bernstein HS, Coughlin SR (Feb 1997). "Pombe Cdc5-related protein. A putative human transcription factor implicated in mitogen-activated signaling". The Journal of Biological Chemistry. 272 (9): 5833–7. doi: 10.1074/jbc.272.9.5833 . PMID   9038199.
7. "Entrez Gene: CDC5L CDC5 cell division cycle 5-like (S. pombe)".
8. Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (Dec 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". The EMBO Journal. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC   305846 . PMID   11101529.
9. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3: 89. doi:10.1038/msb4100134. PMC   1847948 . PMID   17353931.
10. Ajuh P, Lamond AI (Nov 2003). "Identification of peptide inhibitors of pre-mRNA splicing derived from the essential interaction domains of CDC5L and PLRG1". Nucleic Acids Research. 31 (21): 6104–16. doi:10.1093/nar/gkg817. PMC   275459 . PMID   14576297.
11. Ajuh P, Sleeman J, Chusainow J, Lamond AI (Nov 2001). "A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing". The Journal of Biological Chemistry. 276 (45): 42370–81. doi: 10.1074/jbc.M105453200 . PMID   11544257.
12. Leonard D, Ajuh P, Lamond AI, Legerski RJ (Sep 2003). "hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing". Biochemical and Biophysical Research Communications. 308 (4): 793–801. CiteSeerX   10.1.1.539.8359 . doi:10.1016/s0006-291x(03)01486-4. PMID   12927788.

Further reading

• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (Jun 2002). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Research. 9 (3): 99–106. CiteSeerX   10.1.1.500.923 . doi:10.1093/dnares/9.3.99. PMID   12168954.
• Heaney JP, Jacobson A (Aug 1975). "Simplified control of upper abdominal hemorrhage from the vena cava". Surgery. 78 (2): 138–41. PMID   1154257.
• Hirayama T, Shinozaki K (Nov 1996). "A cdc5+ homolog of a higher plant, Arabidopsis thaliana". Proceedings of the National Academy of Sciences of the United States of America. 93 (23): 13371–6. doi: 10.1073/pnas.93.23.13371 . PMC   24100 . PMID   8917598.
• Ishikawa K, Nagase T, Nakajima D, Seki N, Ohira M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Oct 1997). "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 4 (5): 307–13. doi: 10.1093/dnares/4.5.307 . PMID   9455477.
• Bernstein HS, Coughlin SR (Feb 1998). "A mammalian homolog of fission yeast Cdc5 regulates G2 progression and mitotic entry". The Journal of Biological Chemistry. 273 (8): 4666–71. doi: 10.1074/jbc.273.8.4666 . PMID   9468527.
• Ohi R, Feoktistova A, McCann S, Valentine V, Look AT, Lipsick JS, Gould KL (Jul 1998). "Myb-related Schizosaccharomyces pombe cdc5p is structurally and functionally conserved in eukaryotes". Molecular and Cellular Biology. 18 (7): 4097–108. doi:10.1128/mcb.18.7.4097. PMC   108994 . PMID   9632794.
• Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M (Sep 1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nature Genetics. 20 (1): 46–50. doi:10.1038/1700. PMID   9731529. S2CID   585778.
• Burns CG, Ohi R, Krainer AR, Gould KL (Nov 1999). "Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing". Proceedings of the National Academy of Sciences of the United States of America. 96 (24): 13789–94. Bibcode:1999PNAS...9613789B. doi: 10.1073/pnas.96.24.13789 . PMC   24143 . PMID   10570151.
• Boudrez A, Beullens M, Groenen P, Van Eynde A, Vulsteke V, Jagiello I, Murray M, Krainer AR, Stalmans W, Bollen M (Aug 2000). "NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry". The Journal of Biological Chemistry. 275 (33): 25411–7. doi: 10.1074/jbc.M001676200 . PMID   10827081.
• Lei XH, Shen X, Xu XQ, Bernstein HS (Dec 2000). "Human Cdc5, a regulator of mitotic entry, can act as a site-specific DNA binding protein". Journal of Cell Science. 113. 113 (24): 4523–31. doi:10.1242/jcs.113.24.4523. PMID   11082045.
• Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (Dec 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". The EMBO Journal. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC   305846 . PMID   11101529.
• Harrington JJ, Sherf B, Rundlett S, Jackson PD, Perry R, Cain S, Leventhal C, Thornton M, Ramachandran R, Whittington J, Lerner L, Costanzo D, McElligott K, Boozer S, Mays R, Smith E, Veloso N, Klika A, Hess J, Cothren K, Lo K, Offenbacher J, Danzig J, Ducar M (May 2001). "Creation of genome-wide protein expression libraries using random activation of gene expression". Nature Biotechnology. 19 (5): 440–5. doi:10.1038/88107. PMID   11329013. S2CID   25064683.
• Ajuh P, Sleeman J, Chusainow J, Lamond AI (Nov 2001). "A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing". The Journal of Biological Chemistry. 276 (45): 42370–81. doi: 10.1074/jbc.M105453200 . PMID   11544257.
• Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ (Apr 2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC   1370266 . PMID   11991638.
• Leonard D, Ajuh P, Lamond AI, Legerski RJ (Sep 2003). "hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing". Biochemical and Biophysical Research Communications. 308 (4): 793–801. CiteSeerX   10.1.1.539.8359 . doi:10.1016/S0006-291X(03)01486-4. PMID   12927788.
• Liu L, Gräub R, Hlaing M, Epting CL, Turck CW, Xu XQ, Zhang L, Bernstein HS (2004). "Distinct domains of human CDC5 direct its nuclear import and association with the spliceosome". Cell Biochemistry and Biophysics . 39 (2): 119–32. doi:10.1385/CBB:39:2:119. PMID   14515018. S2CID   22472390.

External links

• Human CDC5L genome location and CDC5L gene details page in the UCSC Genome Browser .