SPEN

For other uses, see Spen (disambiguation).

SPEN
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1OW1, 2RT5, 4P6Q
Identifiers
Aliases	SPEN , HIAA0929, MINT, RBM15C, SHARP, spen family transcriptional repressor, RATARS
External IDs	OMIM: 613484 MGI: 1891706 HomoloGene: 124461 GeneCards: SPEN
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1p36.21-p36.13 Start 15,836,095 bp [1] End 15,940,456 bp [1]
Gene location (Mouse) Chr. Chromosome 4 (mouse) [2] Band 4|4 D3 Start 141,195,201 bp [2] End 141,265,908 bp [2]
RNA expression pattern Bgee Top expressed in vagina popliteal artery cerebellar hemisphere prosencephalon corpus callosum More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding GO:0001948 protein binding nucleic acid binding RNA binding GO:0001106 transcription corepressor activity Cellular component transcription repressor complex extracellular exosome nucleus nucleoplasm Biological process Notch signaling pathway positive regulation of neurogenesis mRNA splicing, via spliceosome GO:0022415 viral process negative regulation of transcription, DNA-templated regulation of transcription, DNA-templated negative regulation of transcription by RNA polymerase II transcription, DNA-templated Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	23013	56381
Ensembl	ENSG00000065526	ENSMUSG00000040761
UniProt	Q96T58	Q62504
RefSeq (mRNA)	NM_015001	NM_019763 NM_001347235
RefSeq (protein)	NP_055816	NP_001334164 NP_062737
Location (UCSC)	Chr 1: 15.84 – 15.94 Mb	Chr 4: 141.2 – 141.27 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Msx2-interacting protein is a protein that in humans is encoded by the SPEN gene. ^{[5] [6] [7]}

This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. ^[7]

Interactions

SPEN has been shown to interact with HDAC1, ^[6] SRA1 ^[6] and Nuclear receptor co-repressor 2. ^[6]

References

1. GRCh38: Ensembl release 89: ENSG00000065526 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000040761 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Newberry EP, Latifi T, Towler DA (Sep 1999). "The RRM domain of MINT, a novel Msx2 binding protein, recognizes and regulates the rat osteocalcin promoter". Biochemistry. 38 (33): 10678–90. doi:10.1021/bi990967j. PMID   10451362.
6. Shi Y, Downes M, Xie W, Kao HY, Ordentlich P, Tsai CC, Hon M, Evans RM (May 2001). "Sharp, an inducible cofactor that integrates nuclear receptor repression and activation". Genes Dev. 15 (9): 1140–51. doi:10.1101/gad.871201. PMC   312688 . PMID   11331609.
7. "Entrez Gene: SPEN spen homolog, transcriptional regulator (Drosophila)".

Further reading

• Ternovskaia LN, Prokhorova LG, Moroz VM (1977). "[Antagonistic interrelationships between the staphylococci and Sarcina isolated from the upper respiratory tracts of virtually healthy persons]". Antibiotiki. 21 (12): 1072–5. PMID   1020939.
• Nagase T, Ishikawa K, Suyama M, et al. (1999). "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 6 (1): 63–70. doi: 10.1093/dnares/6.1.63 . PMID   10231032.
• Shi Y, Hon M, Evans RM (2002). "The peroxisome proliferator-activated receptor delta, an integrator of transcriptional repression and nuclear receptor signaling". Proc. Natl. Acad. Sci. U.S.A. 99 (5): 2613–8. Bibcode:2002PNAS...99.2613S. doi: 10.1073/pnas.052707099 . PMC   122396 . PMID   11867749.
• Oswald F, Kostezka U, Astrahantseff K, et al. (2002). "SHARP is a novel component of the Notch/RBP-Jkappa signalling pathway". EMBO J. 21 (20): 5417–26. doi:10.1093/emboj/cdf549. PMC   129081 . PMID   12374742.
• Ariyoshi M, Schwabe JW (2003). "A conserved structural motif reveals the essential transcriptional repression function of Spen proteins and their role in developmental signaling". Genes Dev. 17 (15): 1909–20. doi:10.1101/gad.266203. PMC   196244 . PMID   12897056.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Dotzlaw H, Papaioannou M, Moehren U, et al. (2004). "Agonist-antagonist induced coactivator and corepressor interplay on the human androgen receptor". Mol. Cell. Endocrinol. 213 (1): 79–85. doi:10.1016/j.mce.2003.10.036. PMID   15062576. S2CID   46437905.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi: 10.1073/pnas.0404720101 . PMC   514446 . PMID   15302935.
• Vadlamudi RK, Manavathi B, Singh RR, et al. (2005). "An essential role of Pak1 phosphorylation of SHARP in Notch signaling". Oncogene. 24 (28): 4591–6. doi: 10.1038/sj.onc.1208672 . PMID   15824732.
• Li J, Wang J, Yang X, et al. (2007). "The Spen homolog Msx2-interacting nuclear target protein interacts with the E2 ubiquitin-conjugating enzyme UbcH8". Mol. Cell. Biochem. 288 (1–2): 151–7. doi:10.1007/s11010-006-9131-9. PMID   16583136. S2CID   24935755.
• Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315–21. Bibcode:2006Natur.441..315G. doi: 10.1038/nature04727 . PMID   16710414.
• Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi: 10.1016/j.cell.2006.03.032 . PMID   16713569. S2CID   13709685.
• Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID   16964243. S2CID   14294292.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi: 10.1016/j.cell.2006.09.026 . PMID   17081983. S2CID   7827573.
• Feng Y, Bommer GT, Zhai Y, et al. (2007). "Drosophila split ends homologue SHARP functions as a positive regulator of Wnt/beta-catenin/T-cell factor signaling in neoplastic transformation". Cancer Res. 67 (2): 482–91. doi: 10.1158/0008-5472.CAN-06-2314 . PMID   17234755.