Spirit blue

Spirit blue
Names
	IUPAC name 4-[(4-anilinophenyl)-(4-phenyliminocyclohexa-2,5-dien-1-ylidene)methyl]-N-phenylaniline hydrochloride
	Other names Opal blue SS, HSDB 466, Solvent Blue 23 monohydrochloride
Identifiers
CAS Number	2152-64-9 ;
3D model (JSmol)	Interactive image ;
ChEBI	CHEBI:86483 ;
ChemSpider	15674 ;
ECHA InfoCard	100.016.766
EC Number	218-441-4;
PubChem CID	16534 ;
UNII	690DO205AX ;
CompTox Dashboard (EPA)	DTXSID3027457 ;
	InChI InChI=1S/C37H29N3.ClH/c1-4-10-31(11-5-1)38-34-22-16-28(17-23-34)37(29-18-24-35(25-19-29)39-32-12-6-2-7-13-32)30-20-26-36(27-21-30)40-33-14-8-3-9-15-33;/h1-27,38-39H;1H Key: JMCKWTQLJNQCTD-UHFFFAOYSA-N;
	SMILES C1=CC=C(C=C1)NC2=CC=C(C=C2)C(=C3C=CC(=NC4=CC=CC=C4)C=C3)C5=CC=C(C=C5)NC6=CC=CC=C6.Cl;
Properties
Chemical formula	C37H30ClN3
Molar mass	552.12 g·mol−1
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). Infobox references

Last updated August 29, 2025

Spirit blue or Opal blue SS is a dye with formula C₃₇H₃₀ClN₃.^[1] It is used as an indicator in agar for detection of lipase activity in bacteria.^[2]

References

↑ "Opal Blue SS". National Center for Biotechnology Information. Archived from the original on 21 December 2016. Retrieved 21 December 2016.
↑ Starr, Mortimer P. (April 4, 1941). "Spirit Blue Agar: A Medium for the Detection of Lipolytic Microorganisms" . Science. 93 (2414): 333–334. Bibcode:1941Sci....93..333S. doi:10.1126/science.93.2414.333. PMID 17748334 – via CrossRef.

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[pubchem-1] "Opal Blue SS". National Center for Biotechnology Information. Archived from the original on 21 December 2016. Retrieved 21 December 2016.

[2] Starr, Mortimer P. (April 4, 1941). "Spirit Blue Agar: A Medium for the Detection of Lipolytic Microorganisms" . Science. 93 (2414): 333–334. Bibcode:1941Sci....93..333S. doi:10.1126/science.93.2414.333. PMID 17748334 – via CrossRef.

[1]

[2]

Names

IUPAC name 4-[(4-anilinophenyl)-(4-phenyliminocyclohexa-2,5-dien-1-ylidene)methyl]-N-phenylaniline hydrochloride
Other names Opal blue SS, HSDB 466, Solvent Blue 23 monohydrochloride
Identifiers
CAS Number	2152-64-9 ^Y
3D model (JSmol)	Interactive image
ChEBI	CHEBI:86483 ^Y
ChemSpider	15674 ^Y
ECHA InfoCard	100.016.766
EC Number	218-441-4
PubChem CID	16534
UNII	690DO205AX ^Y
CompTox Dashboard (EPA)	DTXSID3027457
InChI InChI=1S/C37H29N3.ClH/c1-4-10-31(11-5-1)38-34-22-16-28(17-23-34)37(29-18-24-35(25-19-29)39-32-12-6-2-7-13-32)30-20-26-36(27-21-30)40-33-14-8-3-9-15-33;/h1-27,38-39H;1H Key: JMCKWTQLJNQCTD-UHFFFAOYSA-N
SMILES C1=CC=C(C=C1)NC2=CC=C(C=C2)C(=C3C=CC(=NC4=CC=CC=C4)C=C3)C5=CC=C(C=C5)NC6=CC=CC=C6.Cl
Properties
Chemical formula	C₃₇H₃₀ClN₃
Molar mass	552.12 g·mol⁻¹
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). Infobox references