Stygiellidae

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Stygiellidae
Stygiella incarcerata protargol-stained 2015.jpg
Stygiella incarcerata under light microscopy stained with protargol
Velundella nauta DIC 2015.jpg
Velundella nauta under DIC microscopy
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Class: Jakobea
Order: Jakobida
Suborder: Andalucina
Family: Stygiellidae
Pánek, Táborský & Čepička 2015 [1]
Type genus
Stygiella
Pánek, Táborský & Čepička 2015
Genera [1]
Diversity
6 species
Stygiellidae distribution map 2015.jpg
Stygiellidae distribution map. [1]

Stygiellidae is a family of free-living marine flagellates belonging to the order Jakobida, a deep-branching lineage within the eukaryotic supergroup Discoba. They are unicellular organisms that commonly inhabit anoxic, sulfide-rich and ammonium-rich marine habitats worldwide. [1]

Cell morphology and behavior

Members of Stygiellidae are genetically diverse but morphologically similar anaerobic jakobids. The unifying aspect of their appearance is their cristae-lacking mitochondria due to the secondary loss of aerobic metabolism. They resemble jakobid cells, and form two morphotypes: grooved cells, attached to the substrate, with a conspicuous groove; and swimming cells, with a less distinct, narrower groove. Both morphotypes move in a spiral motion, although the swimming cells are faster. It is difficult to distinguish morphological aspects between species due to the variability among cells within the same strain and the similarity between grooved and swimming cells. [1]

Ecology and distribution

Stygiellidae are bacterivorous nanoflagellates almost exclusively found in marine, oxygen-poor (anoxic or microoxic) environments, often in presence of sulfides, methane or ammonium. However, some environmental sequences of Stygiellidae have been reported in oxic waters in Saanich Inlet, without sulfide or ammonium. They are also detected in other non-marine saline environments such as brackish waters and inland salt springs, but never in freshwater habitats. [1]

Evolution and classification

Evolution of the anaerobic metabolism

The anaerobic Stygiellidae is one of two clades of the suborder Andalucina, the other clade being the aerobic Andaluciidae. Andalucina, along with Histionina and Ophirinina, are the two lineages of Jakobida, an important group in the supergroup Discoba. [1] [2]

Jakobida

Stygiellidae, unlike the rest of jakobids, have evolved to adopt an obligatory anaerobic lifestyle, and possess mitochondrion-related organelles (MROs) that lack cristae. They appear to lack all components of the electron transport chain and the TCA cycle, except for two complex I subunits, and no mitochondrial genome has been found. Instead, the MROs possess components of the pyruvate metabolism similar to the hydrogenosomes seen in Metamonada, another basal anaerobic group of eukaryotes. Overall, Stygiellidae appear to have retained a wider group of genes for the mitochondrial aminoacid metabolism than metamonads. [3]

Internal phylogeny

Stygiellidae is a monophyletic group containing all jakobids detected in anoxic habitats. It splits into six well-supported clades: Stygiella , Velundella and four environmental clades (named EC I–IV), all of which appear strongly monophyletic. [1]

Classification

The family contains 6 species distributed in 2 genera. [1]

Related Research Articles

<span class="mw-page-title-main">Percolozoa</span> Phylum of Excavata

The Percolozoa are a group of colourless, non-photosynthetic Excavata, including many that can transform between amoeboid, flagellate, and cyst stages.

<span class="mw-page-title-main">Excavata</span> Supergroup of unicellular organisms belonging to the domain Eukaryota

Excavata is an extensive and diverse but paraphyletic group of unicellular Eukaryota. The group was first suggested by Simpson and Patterson in 1999 and the name latinized and assigned a rank by Thomas Cavalier-Smith in 2002. It contains a variety of free-living and symbiotic protists, and includes some important parasites of humans such as Giardia and Trichomonas. Excavates were formerly considered to be included in the now obsolete Protista kingdom. They were distinguished from other lineages based on electron-microscopic information about how the cells are arranged. They are considered to be a basal flagellate lineage.

<span class="mw-page-title-main">Metamonad</span> Phylum of excavate protists

The metamonads are a large group of flagellate amitochondriate microscopic eukaryotes. Their composition is not entirely settled, but they include the retortamonads, diplomonads, and possibly the parabasalids and oxymonads as well. These four groups are all anaerobic, occurring mostly as symbiotes or parasites of animals, as is the case with Giardia lamblia which causes diarrhea in mammals.

The Oxymonads are a group of flagellated protists found exclusively in the intestines of animals, mostly termites and other wood-eating insects. Along with the similar parabasalid flagellates, they harbor the symbiotic bacteria that are responsible for breaking down cellulose. There is no evidence for presence of mitochondria in oxymonads and 3 species have been shown to completely lack any molecular markers of mitochondria.

A mitosome is a mitochondrion-related organelle (MRO) found in a variety of parasitic unicellular eukaryotes, such as members of the supergroup Excavata. The mitosome was first discovered in 1999 in Entamoeba histolytica, an intestinal parasite of humans, and mitosomes have also been identified in several species of Microsporidia and in Giardia intestinalis.

<i>Breviata</i> Genus of flagellated amoebae

Breviata anathema is a single-celled flagellate amoeboid eukaryote, previously studied under the name Mastigamoeba invertens. The cell lacks mitochondria, much like the pelobionts to which the species was previously assigned, but has remnant mitochondrial genes, and possesses an organelle believed to be a modified anaerobic mitochondrion, similar to the mitosomes and hydrogenosomes found in other eukaryotes that live in low-oxygen environments.

Trimastix is a genus of excavate protists, the sole occupant of the order Trimastigida. Trimastix are bacterivorous, free living and anaerobic. It was first observed in 1881 by William Kent. There are few known species, and the genus's role in the ecosystem is largely unknown. However, it is known that they generally live in marine environments within the tissues of decaying organisms to maintain an anoxic environment. Much interest in this group is related to its close association with other members of Preaxostyla. These organisms do not have classical mitochondria, and as such, much of the research involving these microbes is aimed at investigating the evolution of mitochondria.

<i>Malawimonas</i> Genus of micro-organisms

Malawimonas is genus of unicellular, heterotrophic flagellates with uncertain phylogenetic affinities. They have variably being assigned to Excavata and Loukozoa. Recent studies suggest they may be closely related to the Podiata.

<i>Stygiella incarcerata</i> Species of marine flagellate

Stygiella incarcerata is a species of Excavata.

<span class="mw-page-title-main">Jakobid</span>

Jakobids are an order of free-living, heterotrophic, flagellar eukaryotes in the supergroup Excavata. They are small, and can be found in aerobic and anaerobic environments. The order Jakobida, believed to be monophyletic, consists of only twenty species at present, and was classified as a group in 1993. There is ongoing research into the mitochondrial genomes of jakobids, which are unusually large and bacteria-like, evidence that jakobids may be important to the evolutionary history of eukaryotes.

Gruberellidae is a family of Heterolobosea, Its nucleolus fragments during mitosis, can be uni or multinucleated, has flagellated forms in genera Stachyamoeba. Gruberella, Stachyamoeba.

<i>Mastigamoeba</i> Genus of flagellar amoeboids

Mastigamoeba is a genus of pelobionts, and treated by some as members of the Archamoebae group of protists. Mastigamoeba are characterized as anaerobic, amitochondriate organisms that are polymorphic. Their dominant life cycle stage is as an amoeboid flagellate. Species are typically free living, though endobiotic species have been described.

Psalteriomonas is a genus of excavates in the group of Heterolobosea. The genus was first discovered and named in 1990. It contains amoeboflagellate cells that live in freshwater anaerobic sediments all over the world. The microtubule-organizing ribbon and the associated microfibrillar bundles of the mastigote system is the predominant feature in Psalteriomonas. This harp-shaped complex gives rise to the name of this genus. Psalteriomonasforms an endosymbiotic relationship with methanogenic bacteria, especially with Methanobacterium formicicum There are currently three species in this genus: P. lanterna, P. vulgaris, and P. magna.

<i>Jakoba</i> Genus of Eukaryotic Organisms

Jakoba is a genus in the taxon Excavata, and currently has a single described species, Jakoba libera described by Patterson in 1990, and named in honour of Dutch botanist Jakoba Ruinen.

Andalucia is a genus of jakobids, currently containing the sole species A. godoyi.

Stygiella /ˌstɪ.d͡ʒiˈɛ.lə/ is a genus of free-living marine flagellates belonging to the family Stygiellidae in the jakobids (excavata).

Anaeramoeba is a genus of anaerobic protists of uncertain phylogenetic position, first described in 2016.

<span class="mw-page-title-main">Malawimonad</span> Order of flagellates

Malawimonads are a small group of microorganisms with a basal position in the evolutionary tree of eukaryotes, containing only three recognized species. They're considered part of a paraphyletic group known as "Excavata".

Paratrimastix is a genus of free-living freshwater anaerobic excavate protists from the group Metamonada, that was segregated from the genus Trimastix in 2015. The best studied species is Paratrimastix pyriformis.

<i>Paratrimastix pyriformis</i> Species of protists

Paratrimastix pyriformis is a species of free-living anaerobic freshwater bacteriovorous flagellated protists formerly known as Trimastix pyriformis and Tetramitus pyriformis.

References

  1. 1 2 3 4 5 6 7 8 9 Pánek T, Táborský P, Pachiadaki MG, Hroudová M, Vlček Č, Edgcomb VP, Čepička I (2015). "Combined Culture-Based and Culture-Independent Approaches Provide Insights into Diversity of Jakobids, an Extremely Plesiomorphic Eukaryotic Lineage". Frontiers in Microbiology. 6: 1288. doi: 10.3389/fmicb.2015.01288 . PMC   4649034 . PMID   26635756.
  2. Yabuki A, Gyaltshen Y, Heiss AA, Fujikura K, Kim E (2018). "Ophirina amphinema n. gen., n. sp., a New Deeply Branching Discobid with Phylogenetic Affinity to Jakobids". Sci Rep. 8 (1): 16219. Bibcode:2018NatSR...816219Y. doi:10.1038/s41598-018-34504-6. PMC   6212452 . PMID   30385814.
  3. Leger MM, Kolísko M, Stairs CW, Simpson AGB (2019). "Mitochondrion-Related Organelles in Free-Living Protists". In Tachezy J (ed.). Hydrogenosomes and Mitosomes: Mitochondria of Anaerobic Eukaryotes. Microbiology Monographs. Vol. 9. Springer, Cham. pp. 287–308. doi:10.1007/978-3-030-17941-0_12. hdl:10261/208216. ISBN   978-3-030-17941-0. S2CID   202018954.
  4. Lara E, Chatzinotas A, Simpson AGB (2006). "Andalucia (n. gen.)—the Deepest Branch Within Jakobids (Jakobida; Excavata), Based on Morphological and Molecular Study of a New Flagellate from Soil". Journal of Eukaryotic Microbiology. 53 (2): 112–120. doi:10.1111/j.1550-7408.2005.00081.x. PMID   16579813. S2CID   19092265.
  5. Bernard C, Simpson AGB, Patterson DJ (2000). "Some free-living flagellates (protista) from anoxic habitats". Ophelia. 52 (2): 113–142. doi:10.1080/00785236.1999.10409422. S2CID   84902557.