TH1L

NELFCD
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 5L3X
Identifiers
Aliases	NELFCD , NELF-C, NELF-D, TH1, TH1L, HSPC130, negative elongation factor complex member C/D
External IDs	OMIM: 605297; MGI: 1926424; HomoloGene: 9496; GeneCards: NELFCD; OMA:NELFCD - orthologs
Gene location (Human) Chr. Chromosome 20 (human) [1] Band 20q13.32 Start 58,981,208 bp [1] End 58,995,133 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2|2 H4 Start 174,257,597 bp [2] End 174,269,295 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in anterior pituitary ventricular zone ganglionic eminence right lobe of thyroid gland left lobe of thyroid gland left ovary right hemisphere of cerebellum right ovary islet of Langerhans right uterine tube Top expressed in yolk sac ventricular zone otic vesicle superior cervical ganglion neural layer of retina genital tubercle epiblast otic placode saccule tail of embryo More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding RNA binding Cellular component membrane NELF complex nucleus nucleoplasm Biological process transcription elongation from RNA polymerase II promoter positive regulation of viral transcription negative regulation of transcription, DNA-templated regulation of transcription, DNA-templated transcription by RNA polymerase II transcription, DNA-templated negative regulation of transcription elongation from RNA polymerase II promoter Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 51497 57314 Ensembl ENSG00000101158 ENSMUSG00000016253 UniProt Q8IXH7 Q922L6 RefSeq (mRNA) NM_016397 NM_198976 NM_020580 RefSeq (protein) NP_945327 NP_065605 Location (UCSC) Chr 20: 58.98 – 59 Mb Chr 2: 174.26 – 174.27 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Negative elongation factor C/D is a protein that in humans is encoded by the TH1L gene. ^{[5] [6] [7]}

Function

The NELF^{[ clarification needed ]} complex of proteins interacts with the DSIF^{[ clarification needed ]} protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini. ^[7]

Interactions

TH1L has been shown to interact with:

• ARAF, ^{[8] [9]}
• Cofactor of BRCA1, ^{[10] [11]} and
• RDBP. ^{[10] [11]}

References

1. GRCh38: Ensembl release 89: ENSG00000101158 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000016253 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Bonthron DT, Hayward BE, Moran V, Strain L (Aug 2000). "Characterization of TH1 and CTSZ, two non-imprinted genes downstream of GNAS1 in chromosome 20q13". Human Genetics. 107 (2): 165–75. doi:10.1007/s004390000344. PMID   11030415. S2CID   25570066.
6. Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, Fu G, Shen Y, Fan HY, Lu G, Zhong M, Xu XR, Han ZG, Zhang JW, Tao J, Huang QH, Zhou J, Hu GX, Gu J, Chen SJ, Chen Z (Oct 2000). "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells". Genome Research. 10 (10): 1546–60. doi:10.1101/gr.140200. PMC   310934 . PMID   11042152.
7. "Entrez Gene: TH1L TH1-like (Drosophila)".
8. Yuryev A, Wennogle LP (Feb 2003). "Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis". Genomics. 81 (2): 112–25. doi:10.1016/s0888-7543(02)00008-3. PMID   12620389.
9. Yin XL, Chen S, Gu JX (Feb 2002). "Identification of TH1 as an interaction partner of A-Raf kinase". Molecular and Cellular Biochemistry. 231 (1–2): 69–74. doi:10.1023/A:1014437024129. PMID   11952167. S2CID   19362635.
10. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3: 89. doi:10.1038/msb4100134. PMC   1847948 . PMID   17353931.
11. Narita T, Yamaguchi Y, Yano K, Sugimoto S, Chanarat S, Wada T, Kim DK, Hasegawa J, Omori M, Inukai N, Endoh M, Yamada T, Handa H (Mar 2003). "Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex". Molecular and Cellular Biology. 23 (6): 1863–73. doi:10.1128/mcb.23.6.1863-1873.2003. PMC   149481 . PMID   12612062.

Further reading

• Stevens M, De Clercq E, Balzarini J (Sep 2006). "The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention". Medicinal Research Reviews. 26 (5): 595–625. doi:10.1002/med.20081. PMC   7168390 . PMID   16838299.
• Bonaldo MF, Lennon G, Soares MB (Sep 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi: 10.1101/gr.6.9.791 . PMID   8889548.
• Yamaguchi Y, Takagi T, Wada T, Yano K, Furuya A, Sugimoto S, Hasegawa J, Handa H (Apr 1999). "NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation". Cell. 97 (1): 41–51. doi: 10.1016/S0092-8674(00)80713-8 . PMID   10199401. S2CID   16855257.
• Ping YH, Rana TM (Apr 2001). "DSIF and NELF interact with RNA polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation". The Journal of Biological Chemistry. 276 (16): 12951–8. doi: 10.1074/jbc.M006130200 . PMID   11112772.
• Yin XL, Chen S, Gu JX (Feb 2002). "Identification of TH1 as an interaction partner of A-Raf kinase". Molecular and Cellular Biochemistry. 231 (1–2): 69–74. doi:10.1023/A:1014437024129. PMID   11952167. S2CID   19362635.
• Narita T, Yamaguchi Y, Yano K, Sugimoto S, Chanarat S, Wada T, Kim DK, Hasegawa J, Omori M, Inukai N, Endoh M, Yamada T, Handa H (Mar 2003). "Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex". Molecular and Cellular Biology. 23 (6): 1863–73. doi:10.1128/MCB.23.6.1863-1873.2003. PMC   149481 . PMID   12612062.
• Yuryev A, Wennogle LP (Feb 2003). "Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis". Genomics. 81 (2): 112–25. doi:10.1016/S0888-7543(02)00008-3. PMID   12620389.
• Liu W, Shen X, Yang Y, Yin X, Xie J, Yan J, Jiang J, Liu W, Wang H, Sun M, Zheng Y, Gu J (Mar 2004). "Trihydrophobin 1 is a new negative regulator of A-Raf kinase". The Journal of Biological Chemistry. 279 (11): 10167–75. doi: 10.1074/jbc.M307994200 . PMID   14684750.
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM (Jul 2004). "Functional proteomics mapping of a human signaling pathway". Genome Research. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC   442148 . PMID   15231748.
• Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (Sep 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl: 11858/00-001M-0000-0010-8592-0 . PMID   16169070. S2CID   8235923.
• Yang Y, Liu W, Zou W, Wang H, Zong H, Jiang J, Wang Y, Gu J (May 2007). "Ubiquitin-dependent proteolysis of trihydrophobin 1 (TH1) by the human papilloma virus E6-associated protein (E6-AP)". Journal of Cellular Biochemistry. 101 (1): 167–80. doi:10.1002/jcb.21164. PMID   17131388. S2CID   23675528.