Vibrio natriegens

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Vibrio natriegens
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Bacteria
Phylum: Pseudomonadota
Class: Gammaproteobacteria
Order: Vibrionales
Family: Vibrionaceae
Genus: Vibrio
Species:
V. natriegens
Binomial name
Vibrio natriegens
Synonyms [1] [4]

Pseudomonas natriegens [1]
Beneckea natriegens

Vibrio natriegens is a Gram-negative marine bacterium. [3] [5] It was first isolated from salt marsh mud. It is a salt-loving organism (halophile) requiring about 2% NaCl for growth. It reacts well to the presence of sodium ions which appear to stimulate growth in Vibrio species, to stabilise the cell membrane, and to affect sodium-dependent transport and mobility. Under optimum conditions, and all nutrients provided, the doubling time of V. natriegens can be less than 10 minutes. V. natriegens is able to successfully live and rapidly divide in its coastal areas due its large range of metabolic fuel. Recent research has displayed that Vibrio natriegens has a flexible metabolism, which allows it to consume a large variety of carbon substrates, reduce nitrates, and even fix nitrogen from the atmosphere under nitrogen-limiting and anaerobic conditions. [6] In the laboratory, the growth medium can be easily changed, thus affecting the growth rate of a culture. [7] [8] V. natriegens is commonly found in estuarine mud.

Contents

Aquaculture and antibiotic resistance

Many strains of Vibrio, including natriegens, are pathogenic against farmed aquacultures such as the abalone and have recently resulted in destruction of farmed abalones when aquacultures get infected. [9]  In response, fishers have taken to inoculating tanks with large amounts of antibiotics, which has resulted in Vibrio natriegens developing a potent antibiotic resistance to many drugs. In a recent study, the AbY-1805 strain of Vibrio natriegens was shown to be completely resistant against 17 of the 32 tested antibiotics and at least partially resistant against 22 of the 32. [10]

Biochemical characteristics of V. natriegens

Colony, morphological, physiological, and biochemical characteristics of Vibrio natriegens are shown in the Table below. [5]

Test typeTestCharacteristics
Colony charactersSizeMedium
TypeRound
ColorWhitish
ShapeConvex
Morphological charactersShapeVibrio
Physiological charactersMotility+
Growth at 6.5% NaCl+
Biochemical charactersGram's staining
Oxidase+
Catalase+
Oxidative-FermentativeOxidative
Motility+
Methyl Red
Voges-Proskauer
Indole
H2S Production+
Urease+
Nitrate reductase+
β-Galactosidase+
Hydrolysis ofGelatin+
Aesculin+
Casein+
Tween 40+
Tween 60+
Tween 80+
Acid production from Glycerol +
Galactose +
D-Glucose +
D-Fructose V
D-Mannose V
Mannitol V
N-Acetylglucosamine +
Amygdalin
Maltose +
D-Melibiose
D-Trehalose
Glycogen +
D-Turanose +

Note: + = Positive, – =Negative, V =Variable (+/–)

Biotechnological uses

Owing to its rapid growth rate, ability to grow on inexpensive carbon sources, and capacity to secrete proteins into the growth media, efforts are underway to leverage this species as a host for molecular biology and biotechnology applications. [11] [12] Recently, V. natriegens crude extract has been shown by multiple research groups to be a promising platform for cell-free expression. [13] [14] [15] [16] Scientists are also hoping that Vibrio natriegens, with its incredible growth speed, will make microbial experiments in outer space, where time is an extremely valuable asset, much quicker. Interestingly, it has been shown that Vibrio natriegens, despite its incredibly quick doubling speed on Earth, might grow even faster in space.  A recent experiment displayed that after 24 hours of growth the Vibrio cells grown in zero gravity were 60 times denser than those grown in full gravity, possibly attributable to an extended exponential growth phase in low-gravity conditions. [17]

Related Research Articles

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A flagellum is a hairlike appendage that protrudes from certain plant and animal sperm cells, from fungal spores (zoospores), and from a wide range of microorganisms to provide motility. Many protists with flagella are known as flagellates.

<span class="mw-page-title-main">Biofilm</span> Aggregation of bacteria or cells on a surface

A biofilm is a syntrophic community of microorganisms in which cells stick to each other and often also to a surface. These adherent cells become embedded within a slimy extracellular matrix that is composed of extracellular polymeric substances (EPSs). The cells within the biofilm produce the EPS components, which are typically a polymeric combination of extracellular polysaccharides, proteins, lipids and DNA. Because they have a three-dimensional structure and represent a community lifestyle for microorganisms, they have been metaphorically described as "cities for microbes".

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<span class="mw-page-title-main">Genetic transformation</span> Genetic alteration of a cell by uptake of genetic material from the environment

In molecular biology and genetics, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s). For transformation to take place, the recipient bacterium must be in a state of competence, which might occur in nature as a time-limited response to environmental conditions such as starvation and cell density, and may also be induced in a laboratory.

<i>Bacillus subtilis</i> Catalase-positive bacterium

Bacillus subtilis, known also as the hay bacillus or grass bacillus, is a gram-positive, catalase-positive bacterium, found in soil and the gastrointestinal tract of ruminants, humans and marine sponges. As a member of the genus Bacillus, B. subtilis is rod-shaped, and can form a tough, protective endospore, allowing it to tolerate extreme environmental conditions. B. subtilis has historically been classified as an obligate aerobe, though evidence exists that it is a facultative anaerobe. B. subtilis is considered the best studied Gram-positive bacterium and a model organism to study bacterial chromosome replication and cell differentiation. It is one of the bacterial champions in secreted enzyme production and used on an industrial scale by biotechnology companies.

<i>Aliivibrio fischeri</i> Species of bacterium

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<i>Burkholderia</i> Genus of bacteria

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<i>Aeromonas hydrophila</i> Species of heterotrophic, Gram-negative, bacterium

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Vibrio alginolyticus is a Gram-negative marine bacterium. It is medically important since it causes otitis and wound infection. It is also present in the bodies of animals such as pufferfish, where it is responsible for the production of the potent neurotoxin, tetrodotoxin.

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References

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