Worker bee

Last updated
Worker bees (with queen) Todd Huffman - Lattice (by).jpg
Worker bees (with queen)

A worker bee is any female bee that lacks the reproductive capacity of the colony's queen bee and carries out the majority of tasks needed for the functioning of the hive. While worker bees are present in all eusocial bee species, the term is rarely used (outside of scientific literature) for bees other than honey bees, particularly the European honey bee (Apis mellifera). Worker bees of this variety are responsible for approximately 80% of the world's crop pollination services. [1]

Contents

Worker bees are the caste of bee that perform most of the fundamental tasks of the hive, and they are by far the most numerous type of bee. [2] They are much smaller than drones or queen bees, with bodies specialized for nectar and pollen collection. They perform different tasks around the hive progressively over their lifespans in a predictable order based on their age. [3]

Worker bees gather pollen in the pollen baskets on their back legs and carry it back to the hive where it is used as food for the developing brood. Pollen carried on their bodies may be transferred to another flower, where a small portion can rub off on the pistil, resulting in cross pollination. Nectar is sucked up through the proboscis, mixed with enzymes in the stomach, and carried back to the hive, where it is stored in wax cells and evaporated into honey. [4]

Life cycle

Honey bee workers maintain the hive temperature in the critical brood area where new bees are hatched and raised. Workers must maintain the hive's brood chamber within a range of 34-36 °C (93.2-96.8 °F). If the chamber becomes too hot, the workers collect water or diluted nectar and deposit it around the hive, then fan the air with their wings to generate cooling by evaporation. If the chamber becomes too cold, worker bees can increase the temperature of their thoracic muscles using isometric contractions, pressing their warmed muscles against caps or walls of brood cells. [5] In the wintertime, worker bees can cluster together to generate body heat to keep the brood area warm as external temperature decreases. [6]

The life span of a worker bee fluctuates between the summer and winter months. In the summertime, worker bees typically only live two to six weeks compared to wintertime when workers can live up to 20 weeks. The reason for this difference lies in interior physiological processes that worker bees experience, and external factors, such as bees not leaving the relative safety of the hive during the colder winter months. Worker bees are exposed to a lot more risks during the summer months when they leave the hive to forage, and therefore have a shorter relative lifespan. [7]

Honey bees begin as an egg laid by the queen in the brood nest, located near the center of the hive. Worker eggs are laid in smaller cells compared to drone eggs, and will hatch after three days into a larva. Nurse bees feed it royal jelly for three days, [8] followed by pollen and honey for about two more days until the cell is capped by worker bees. The larva spins itself into a cocoon and becomes an inactive pupa. During this 10-day stage, the bee begins to develop features such as eyes, wings, legs and other features that adult bees possess. After this 21 day developmental period, the adult bee will chew through its wax cap and cocoon and emerge into the hive as a fully grown bee, immediately beginning its roles in the hive. [9]

TypeEggLarvaCell cappedPupaAverage Developmental Period

(Days until emergence)

Start of FertilityBody LengthHatching Weight
Workerup to Day 3up to Day 9Day 9Day 10 until emergence (Day 11 or 12 last moult)21 days

(range: 18–22 days)

N/A12–15 mmnearly 100 mg

Progression of tasks

Through the lifecycle of a worker bee, she will take on many different roles within the hive, depending on how old she is and how long she has been working in the hive. The exact number of days she spends at each task depends on the requirements of the hive, however there is an estimated number of days each worker bee will spend at each task. [10]

Cell cleaning (days 1–2)

Brood cells must be cleaned before the next use. Worker bees in the cleaning phase perform this cleaning. Cells are inspected by the queen and if unsatisfactory, they will not be used. If the cells are not clean, the worker bee must repeat the cleaning process.

Nurse bees (days 3–12)

Nurse bees feed the worker larvae worker jelly, which is secreted from glands that produce royal jelly. On days 6-12, nurse bees feed royal jelly, rich in vitamins, to the queen larva and drones. Drones receive worker jelly for 1 to 3 days until they are started on a diet of honey.

Queen attendants (days 7-11)

Queen attendants take care of the queen by feeding and grooming her. After coming into contact with the queen, the attendants spread queen mandibular pheromone (QMP) throughout the hive, which is a signal to the rest of the bees that the hive still has a viable queen.

Wax production (days 13–18)

Wax bees build cells from wax, repair old cells, and store nectar and pollen brought in by other workers. Early in the worker's career, she exudes wax from the space between her abdominal segments. Four sets of wax glands, situated inside the last four ventral segments of the abdomen, produce wax for comb construction.

Foraging bees (days 21–42)

The forager and scout bees travel up to 3 kilometres (1.9 mi) to a nectar source, pollen source, or to collect propolis or water. Worker bees journey away from the hive around 10 times each day, with each trip lasting an hour. [11]

Worker activities in the hive

Honey production

Worker bees collect nectar from flowers using their tubular mouth parts, and store it in their honey stomach. Enzymes proceed to break down the nectar into simple sugars. Back at the hive, the nectar is distributed to other worker bees who either distribute it to young bees or store it in honeycomb cells. Then, honey is produced by being further dehydrated via fanning then sealed with a wax cap, which prevents absorption of moisture from the air. [12]

Drone feeding

Drones do not feed themselves when they are young; they are fed by workers and then when the drone bees get older they feed themselves from the honey supply. [13]

Honeycomb building

Worker bees have eight pairs of wax glands under their abdomen from which they can create honeycomb. By chewing the wax, she mixes in her saliva, which adjusts the malleability of the wax and enables her to create each individual honeycomb cell. This process is repeated thousands of times to create comb in the hive, which will be used for honey production and brood space. [14]

Pollen packing

Pollen brought into the hive for feeding the brood is also stored. It must be packed firmly into comb cells and mixed with a small amount of honey so that it will not spoil. Unlike honey, which does not support bacterial life, stored pollen will become rancid without proper care. It has to be kept in honey cells. [15]

Propolizing

The walls of the hive are covered with a thin coating of propolis, a resinous substance obtained from plants. When workers add enzymes to the propolis, the combination has antibacterial and antifungal properties. Propolis is placed at the entrance of hives to aid in ventilation.

Some bees add excess mud to the mixture, making it geopropolis, such as in the bee Melipona scutellaris. [16] Geopropolis displays antimicrobial and antiproliferative activity and has been proven to be a source of antibiofilm agents. It also presents selectivity against human cancer cell lines at low concentrations compared to normal cells. [17]

Mortuary bees

Dead bees and failed larvae must be removed from the hive to prevent disease and allow cells to be reused. They will be carried some distance from the hive by mortuary bees. [18]

Fanning bees

Worker bees fan the hive, cooling it with evaporated water. They direct airflow into the hive or out of the hive depending on need. [19]

Water carriers

When the hive is in danger of overheating, these bees will obtain water, usually from within a short distance from the hive and bring it back to spread on the backs of fanning bees. [20]

Guard bees

Guard bees will stand at the front of the hive entrance, defending it from any invaders such as wasps. The number of guards varies from season to season and from species to species. Entrance size and daily traffic also play an integral role in the number of guard bees present. Guard bees of the species Tetragonisca angustula and Schwarziana quadripunctata are examples of eusocial bees that have been observed hovering at their nest entrances, providing more protection against intruders. [21] [22]

Genetic characteristics

In most common bee species, worker bees are infertile due to enforced altruistic kin selection, [23] and thus never reproduce. Workers are nevertheless considered female for anatomical and genetic reasons. Genetically, a worker bee does not differ from a queen bee and can even become a laying worker bee, but in most species will produce only male (drone) offspring. Whether a larva becomes a worker or a queen depends on the kind of food it is given after the first three days of its larval form.

Gut bacteria

The workers perform different behavioral tasks in the colony that cause them to be exposed to different local environments. The worker gut microbial community composition is found to be associated with the behavioral tasks they perform, therefore also with the local environment they are exposed to [24] and the environmental landscape is shown to affect the gut microbial community (gut microbiota composition) of honey bees. [25]

Stinger

The stinger of a black honeybee torn off its body and attached to a protecting dress Bee-sting-abeille-dard-2.jpg
The stinger of a black honeybee torn off its body and attached to a protecting dress

The worker bee's stinger is a complex organ that allows a bee to defend itself and the hive from most mammals. [26] Bee stings against mammals and birds typically leave the stinger embedded in the victim due to the structure of flesh and the stinger's barbs. In this case, the venom bulb stays with the stinger and continues to pump. The bee will die after losing its stinger, as the removal of the stinger and the venom bulb damages or removes other internal organs as well. [27]

The barbs on the stinger will not catch on most animals besides mammals and birds, which means that such animals can be stung repeatedly by the same bee.

Other social bees

There are many types of eusocial bees, including bumble bees, stingless bees, some orchid bees, and many species of sweat bees, native to all continents except for Antarctica, that have workers. Workers in these other bee lineages do not show significant morphological differences from queens, other than coloration or a smaller average body size, though they are often quite different in their behavior from queens, and may or may not lay eggs. See the respective articles for these lineages for details. [28]

Related Research Articles

<span class="mw-page-title-main">Bee</span> Clade of insects

Bees are winged insects closely related to wasps and ants, known for their roles in pollination and, in the case of the best-known bee species, the western honey bee, for producing honey. Bees are a monophyletic lineage within the superfamily Apoidea. They are currently considered a clade, called Anthophila. There are over 20,000 known species of bees in seven recognized biological families. Some species – including honey bees, bumblebees, and stingless bees – live socially in colonies while most species (>90%) – including mason bees, carpenter bees, leafcutter bees, and sweat bees – are solitary.

<span class="mw-page-title-main">Honey bee</span> Colonial flying insect of genus Apis

A honey bee is a eusocial flying insect within the genus Apis of the bee clade, all native to mainland Afro-Eurasia. After bees spread naturally throughout Africa and Eurasia, humans became responsible for the current cosmopolitan distribution of honey bees, introducing multiple subspecies into South America, North America, and Australia.

Beekeeping is the maintenance of bee colonies, commonly in man-made beehives. Honey bees in the genus Apis are the most commonly kept species but other honey producing bees such as Melipona stingless bees are also kept. Beekeepers keep bees to collect honey and other products of the hive: beeswax, propolis, bee pollen, and royal jelly. Other sources of beekeeping income include pollination of crops, raising queens, and production of package bees for sale. Bee hives are kept in an apiary or "bee yard".

<span class="mw-page-title-main">Bee brood</span> Chamber of a beehive

In beekeeping, bee brood or brood refers to the eggs, larvae and pupae of honeybees. The brood of Western honey bees develops within a bee hive. In man-made, removable frame hives, such as Langstroth hives, each frame which is mainly occupied by brood is called a brood frame. Brood frames usually have some pollen and nectar or honey in the upper corners of the frame. The rest of the brood frame cells may be empty or occupied by brood in various developmental stages. During the brood raising season, the bees may reuse the cells from which brood has emerged for additional brood or convert it to honey or pollen storage. Bees show remarkable flexibility in adapting cells to a use best suited for the hive's survival.

<span class="mw-page-title-main">Queen bee</span> Egg-laying individual in a bee colony

A queen bee is typically an adult, mated female (gyne) that lives in a colony or hive of honey bees. With fully developed reproductive organs, the queen is usually the mother of most, if not all, of the bees in the beehive. Queens are developed from larvae selected by worker bees and specially fed in order to become sexually mature. There is normally only one adult, mated queen in a hive, in which case the bees will usually follow and fiercely protect her.

<span class="mw-page-title-main">Bee pollen</span> Ball of pollen gathered by worker honeybees

Bee pollen, also known as bee bread and ambrosia, is a ball or pellet of field-gathered flower pollen packed by worker honeybees, and used as the primary food source for the hive. It consists of simple sugars, protein, minerals and vitamins, fatty acids, and a small percentage of other components. Bee pollen is stored in brood cells, mixed with saliva, and sealed with a drop of honey. Bee pollen is harvested as food for humans and marketed as having various, but yet unproven, health benefits.

Hive management in beekeeping refers to intervention techniques that a beekeeper may perform to ensure hive survival and to maximize hive production. Hive management techniques vary widely depending on the objectives.

This page is a glossary of beekeeping.

<span class="mw-page-title-main">Stingless bee</span> Bee tribe, reduced stingers, strong bites

Stingless bees (SB), sometimes called stingless honey bees or simply meliponines, are a large group of bees (from about 462 to 552 described species), comprising the tribe Meliponini (or subtribe Meliponina according to other authors). They belong in the family Apidae (subfamily Apinae), and are closely related to common honey bees (HB, tribe Apini), orchid bees (tribe Euglossini), and bumblebees (tribe Bombini). These four bee tribes belong to the corbiculate bees monophyletic group. Meliponines have stingers, but they are highly reduced and cannot be used for defense, though these bees exhibit other defensive behaviors and mechanisms. Meliponines are not the only type of bee incapable of stinging: all male bees and many female bees of several other families, such as Andrenidae and Megachilidae (tribe Dioxyini), also cannot sting.

<i>Apis andreniformis</i> Species of bee

Apis andreniformis, or the black dwarf honey bee, is a relatively rare species of honey bee whose native habitat is the tropical and subtropical regions of Southeast Asia.

<span class="mw-page-title-main">Western honey bee</span> European honey bee

The western honey bee or European honey bee is the most common of the 7–12 species of honey bees worldwide. The genus name Apis is Latin for "bee", and mellifera is the Latin for "honey-bearing" or "honey carrying", referring to the species' production of honey.

<i>Apis cerana</i> Species of insect

Apis cerana, the eastern honey bee, Asiatic honey bee or Asian honey bee, is a species of honey bee native to South, Southeast and East Asia. This species is the sister species of Apis koschevnikovi and both are in the same subgenus as the western (European) honey bee, Apis mellifera. A. cerana is known to live sympatrically along with Apis koschevnikovi within the same geographic location. Apis cerana colonies are known for building nests consisting of multiple combs in cavities containing a small entrance, presumably for defense against invasion by individuals of another nest. The diet of this honey bee species consists mostly of pollen and nectar, or honey. Moreover, Apis cerana is known for its highly social behavior, reflective of its classification as a type of honey bee.

<i>Tetragonula carbonaria</i> Species of bee

Tetragonula carbonaria is a stingless bee, endemic to the north-east coast of Australia. Its common name is sugarbag bee. They are also occasionally referred to as bush bees. The bee is known to pollinate orchid species, such as Dendrobium lichenastrum, D. toressae, and D. speciosum. It has been identified as an insect that collects pollen from the cycad Cycas media. They are also known for their small body size, reduced wing venation, and highly developed social structure comparable to honey bees.

<i>Tetragonisca angustula</i> Species of bee

Tetragonisca angustula is a small eusocial stingless bee found in México, Central and South America. It is known by a variety of names in different regions. A subspecies, Tetragonisca angustula fiebrigi, occupies different areas in South America and has a slightly different coloration.

<i>Melipona bicolor</i> Species of bee

Melipona bicolorLepeletier, 1836, commonly known as Guaraipo or Guarupu, is a eusocial bee found primarily in South America. It is an inhabitant of the Araucaria Forest and the Atlantic Rainforest, and is most commonly found from South to East Brazil, Bolivia, Argentina, and Paraguay. It prefers to nest close to the soil, in hollowed trunks or roots of trees. M. bicolor is a member of the tribe Meliponini, and is therefore a stingless bee. This species is unique among the stingless bees species because it is polygynous, which is rare for eusocial bees.

<i>Plebeia remota</i> Species of bee

Plebeia remota is a species of stingless bee that is in the family Apidae and tribe Meliponini. Bees of the species are normally found in a few states in southern Brazil and their nests can be found in tree cavities. Depending on the region, P. remota may have a different morphology and exhibit different behaviors. The bee's diet consists of nectar and pollen that are collected intensely from a few sources. Researchers have conducted a multitude of studies analyzing the changes that occur in the colony during reproductive diapause and what happens during the provisioning and oviposition process or POP.

<i>Melipona beecheii</i> Species of bee

Melipona beecheii is a species of eusocial stingless bee. It is native to Central America from the Yucatán Peninsula in the north to Costa Rica in the south. M. beecheii was cultivated in the Yucatán Peninsula starting in the pre-Columbian era by the ancient Maya civilization. The Mayan name for M. beecheii is xunan kab, which translates roughly to "regal lady bee". M. beecheii serves as the subject of various Mayan religious ceremonies.

<i>Scaptotrigona postica</i> Species of bee

Scaptotrigona postica is a species of stingless bee that lives mainly in Brazil. It is a eusocial bee in the tribe Meliponini. S. postica is one of 25 species in the genus Scaptotrigona and is a critical pollinator of the tropical rain forests of Brazil. They construct their nests in hollowed sections of tree trunks, allowing for effective guarding at the nest entrance. This species shows colony structure similar to most members of the Meliponini tribe with three roles within the colony: queen, worker, and male. S. postica individuals have different forms of communication from cuticular hydrocarbons to pheromones and scent trails. Communication is especially useful during worker foraging for nectar and pollen through the Brazilian tropical rain forests. S. postica is a very important pollinator of the Brazilian tropical rain forests and is widely appreciated for its honey. Stingless bees account for approximately 30% of all pollination of the Brazilian Caatinga and Pantanal ecosystems and up to 90% of the pollination for many species of the Brazilian Atlantic Forest and the Amazon.

<i>Melipona quadrifasciata</i> Species of bee

Melipona quadrifasciata is a species of eusocial, stingless bee of the order Hymenoptera. It is native to the southeastern coastal states of Brazil, where it is more commonly known as mandaçaia, which means "beautiful guard," as there is always a bee at the narrow entrance of the nest. M. quadrifasciata constructs mud hives in the hollows of trees to create thin passages that only allow one bee to pass at a time. Because they are stingless bees, M. quadrifasciata is often used as pollinators in greenhouses, outperforming honey bees in efficiency and leading to overall larger yields of fruits that were heavier, larger, and contained more seeds.

<i>Melipona scutellaris</i> Species of bee

Melipona scutellaris is a eusocial stingless bee species of the order Hymenoptera and the genus Melipona. It is considered to be the reared Melipona species with the largest distribution in the North and Northeast regions of Brazil, with records from Rio Grande do Norte down to Bahia. Its common name, Uruçu, comes from the Tupi "eiru su", which in this indigenous language means "big bee". Their honey is highly desirable and the materials they create for nests have been proven to be a promising source of antibiofilm agents and to present selectivity against human cancer cell lines at low concentrations compared to normal cells.

References

  1. Breeze, T. D.; Bailey, A. P.; Balcombe, K. G.; Potts, S. G. (2011-08-01). "Pollination services in the UK: How important are honeybees?". Agriculture, Ecosystems & Environment. 142 (3): 137–143. Bibcode:2011AgEE..142..137B. doi:10.1016/j.agee.2011.03.020. ISSN   0167-8809. S2CID   83628314.
  2. "About Honey Bees | Types, races, and anatomy of honey bees". About Honey Bees | Types, races, and anatomy of honey bees. Retrieved 2024-03-25.
  3. "ENY-166/IN1102: The Social Organization of Honey Bees". Ask IFAS - Powered by EDIS. Retrieved 2024-03-25.
  4. "Bee Life Stages". 2006-12-31. Archived from the original on 2006-12-31. Retrieved 2023-11-28.
  5. Li, Zhiyong; Huang, Zachary Y.; Sharma, Dhruv B.; Xue, Yunbo; Wang, Zhi; Ren, Bingzhong (2016-02-16). "Drone and Worker Brood Microclimates Are Regulated Differentially in Honey Bees, Apis mellifera". PLOS ONE. 11 (2): e0148740. Bibcode:2016PLoSO..1148740L. doi: 10.1371/journal.pone.0148740 . ISSN   1932-6203. PMC   4755576 . PMID   26882104.
  6. "Honey Bee Management Throughout the Seasons". extension.psu.edu. Retrieved 2024-03-06.
  7. Prado, Alberto; Requier, Fabrice; Crauser, Didier; Le Conte, Yves; Bretagnolle, Vincent; Alaux, Cédric (November 2020). "Honeybee lifespan: the critical role of pre-foraging stage". Royal Society Open Science. 7 (11): 200998. Bibcode:2020RSOS....700998P. doi:10.1098/rsos.200998. ISSN   2054-5703. PMC   7735337 . PMID   33391795.
  8. "Royal Jelly", LiverTox: Clinical and Research Information on Drug-Induced Liver Injury, Bethesda (MD): National Institute of Diabetes and Digestive and Kidney Diseases, 2012, PMID   35593875 , retrieved 2024-03-08
  9. "Stages Of Bee Growth – Honey Bee Research Centre". hbrc.ca. Retrieved 2024-03-08.
  10. "Honeybees all have different jobs to accomplish—Here's how they decide who is doing what". Animals. 2019-03-22. Retrieved 2024-03-25.
  11. "Bee Life Stages". 2006-12-31. Archived from the original on 2006-12-31. Retrieved 2023-11-28.
  12. "How Bees Make Honey: Producing honey is a strenuous team effort for bees – CALS News". news.cals.wisc.edu. Retrieved 2024-03-24.
  13. Free, J. B. (1957-01-01). "The food of adult drone honeybees (Apis mellifera)". The British Journal of Animal Behaviour. 5 (1): 7–11. doi:10.1016/S0950-5601(57)80038-0. ISSN   0950-5601.
  14. "The Secrets of Honeycomb - PerfectBee". www.perfectbee.com. Retrieved 2024-03-25.
  15. "The Role of the Worker Bee - PerfectBee". www.perfectbee.com. Retrieved 2024-03-25.
  16. Adriana, Pianaro (2007). "Chemical Changes Associated with the Invasion of a Melipona scutellaris Colony by Melipona rufiventris Workers". Journal of Chemical Ecology. 33 (5): 971–984. Bibcode:2007JCEco..33..971P. doi:10.1007/s10886-007-9274-5. PMID   17404819. S2CID   32195400.
  17. Cunha, Marcos Guilherme da; Franchin, Marcelo; Galvão, LíviaCâmaradeCarvalho; Ruiz, AnaLúciaTascaGóis de; Carvalho, João Ernesto de; Ikegaki, Masarahu; Alencar, Severino Matias de; Koo, Hyun; Rosalen, Pedro Luiz (2013-01-28). "Antimicrobial and antiproliferative activities of stingless bee Melipona scutellaris geopropolis". BMC Complementary and Alternative Medicine. 13 (1): 23. doi: 10.1186/1472-6882-13-23 . ISSN   1472-6882. PMC   3568042 . PMID   23356696.
  18. "Worker Bees - An Overview". AGrowTronics - IIoT For Growing. Retrieved 2024-03-25.
  19. Alvéole (2021-09-09). "Roles of a worker bee". Alvéole. Retrieved 2024-03-25.
  20. "The Role of the Worker Bee - PerfectBee". www.perfectbee.com. Retrieved 2024-03-25.
  21. Segers, Francisca (17 January 2015). "Soldier production in a stingless bee depends on rearing location and nurse behavior". Behavioral Ecology and Sociobiology. 69 (4): 613–623. doi:10.1007/s00265-015-1872-6. S2CID   18594915.
  22. Couvillon, M.J.; Wenseleers, T.; Imperatriz-Fonseca, L.; Nogueira-Neto, P.; Ratnieks, F.L.W. (2007). "Comparative Study in Stingless Bees (Meliponini) Demonstrates that Nest Entrance Size Predicts Traffic and Defensivity" (PDF). Journal of Evolutionary Biology. 21 (1): 194–201. doi: 10.1111/j.1420-9101.2007.01457.x . PMID   18021200.
  23. Ratnieks, F. L. W.; Helantera, H. (2009). "The evolution of extreme altruism and inequality in insect societies". Philosophical Transactions of the Royal Society B: Biological Sciences. 364 (1533): 3169–79. doi:10.1098/rstb.2009.0129. PMC   2781879 . PMID   19805425.
  24. Julia C. Jones, C. Fruciano, J. Marchant, F. Hildebrand, S. Forslund, P. Bork, P. Engel, W. O. H. Hughes (May 2018). "The gut microbiome is associated with behavioural task in honey bees". Insectes Sociaux. 65 (3): 419–429. doi:10.1007/s00040-018-0624-9. PMC   6061168 . PMID   30100619.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  25. Julia C. Jones, C. Fruciano, F. Hildebrand, H. Al Toufalilia, N.J. Balfour, P. Bork, P. Engel, F.L.W. Ratnieks, W.O.H. Hughes (Nov 2017). "Gut microbiota composition is associated with environmental landscape in honey bees". Ecology and Evolution. 8 (1): 441–451. doi:10.1002/ece3.3597. PMC   5756847 . PMID   29321884.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  26. Ramirez-Esquivel, Fiorella; Ravi, Sridhar (Jun 23, 2023). "Functional anatomy of the worker honeybee stinger (Apis mellifera)". 50 iScience. 26 (7). Bibcode:2023iSci...26j7103R. doi:10.1016/j.isci.2023.107103. PMC   10359947 . PMID   37485367.
  27. "Why do honeybees die when they sting?". PBS NewsHour. 2014-10-01. Retrieved 2024-03-25.
  28. Danforth, Bryan N. (2002-01-08). "Evolution of sociality in a primitively eusocial lineage of bees". Proceedings of the National Academy of Sciences. 99 (1): 286–290. Bibcode:2002PNAS...99..286D. doi: 10.1073/pnas.012387999 . ISSN   0027-8424. PMC   117553 . PMID   11782550.