Simian foamy virus

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Simian foamy virus
Virus classification
Group:
Group VI (ssRNA-RT)
Order:
Ortervirales
Family:
Retroviridae
Subfamily:
Spumaretrovirinae
Genus:
Spumavirus
Species:
Simian foamy virus

Simian foamy virus (SFV) is a species of the genus Spumavirus that belongs to the family of Retroviridae . It has been identified in a wide variety of primates, including prosimians, New World and Old World monkeys, as well as apes, and each species has been shown to harbor a unique (species-specific) strain of SFV, including African green monkeys, baboons, macaques, and chimpanzees. [1] As it is related to the more well-known retrovirus human immunodeficiency virus (HIV), its discovery in primates has led to some speculation that HIV may have been spread to the human species in Africa through contact with blood from apes, monkeys, and other primates, most likely through bushmeat-hunting practices.

Contents

Description

Although the simian foamy virus is endemic in African apes and monkeys, there are extremely high infection rates in captivity, ranging from 70% to 100% in adult animals. [1] As humans are in close proximity to infected individuals, people who have had contact with primates can become infected with SFV, making SFV a zoonotic virus. [2] Its ability to cross over to humans was proven in 2004 by a joint United States and Cameroonian team which found the retrovirus in gorillas, mandrills, and guenons; unexpectedly, they also found it in 10 of 1,100 local Cameroon residents. Of those found infected, the majority are males who had been bitten by a primate. While this only accounts for 1% of the population, this detail alarms some who fear the outbreak of another zoonotic epidemic. [3]

SFV causes cells to fuse with each other to form syncytia, whereby the cell becomes multi-nucleated and many vacuoles form, giving it a "foamy" appearance.

Structure

An electron micrograph of foamy virus particles in an unknown sample. Image of Spumavirus.jpg
An electron micrograph of foamy virus particles in an unknown sample.

The SFV is a spherical, enveloped virus that ranges from 80 to 100 nm in diameter. The cellular receptors have not been characterized, but it is hypothesized that it has a molecular structure with near ubiquitous prevalence, since a wide range of cells are permissible to infection. [4]

As a retrovirus, SFV poses the following structural characteristics:

Genome

As a retrovirus, the genomic material is monopartite, linear, positive-sense single-stranded RNA that forms a double stranded DNA intermediate through the use of the enzyme reverse transcriptase. The RNA strand is approximately 12kb's in length, with a 5'-cap and a 3'poly-A tail. The first full genome annotation of a proviral SFV isolated from cynomolgus macaque (Macaca fascicularis) had been performed in December 2016, where it revealed two regulatory sequences, tas and bet, in addition to the structural sequences of gag, pol and env. [6] There are two long terminal repeats (LTRs) of about 600 nucleotides long at the 5' and 3' ends that function as promoters, with an additional internal promoter (IP) located near the 3' end of env. [7] The LTRs contain the U3, R, and U5 regions that are characteristic of retroviruses. There is also a primer binding site (PBS) at the 5'end and a polypurine tract (PPT) at the 3'end. [8]

Whereas gag, pol, and env are conserved throughout retroviruses, the tas gene is unique and found only in Spumaviridae. It encodes for a trans-activator protein required for transcription from both the LTR promoter and the IP. The synthesized Tas protein, which was initially known as Bel-1, is a 36-kDa phosphoprotein which contains an acidic transcription activation domain at its C-terminus and a centrally located DNA binding domain. [7]

The Bet protein is required for viral replication, as it counteracts the innate antiretroviral activity of APOBEC3 family defense factors by obstructing their incorporation into virions. [9]

Replication cycle

Entry into cell

The virus attaches to host receptors through the SU glycoprotein, and the TM glycoprotein mediates fusion with the cell membrane. The entry receptor that triggers viral entry has not been identified, but the absence of heparan sulfate in one study resulted in a decrease of infection, acknowledging it as an attachment factor that assists in mediating the entry of the viral particle. [4] It is not clear if the fusion is pH-dependent or independent, although some evidence has been provided to indicate that SFV does enter cells through a pH-dependent step. [10] Once the virus has entered the interior of the cell, the retroviral core undergoes structural transformations through the activity of viral proteases. Studies have revealed that there are three internal protease-dependent cleavage sites that are critical for the virus to be infectious. One mutation within the gag gene had caused a structural change to the first cleavage site, preventing subsequent cleavage at the two other sites by the viral PR, reflecting its prominent role. [11] Once disassembled, the genetic material and enzymes are free within the cytoplasm to continue with the viral replication. Whereas most retroviruses deposit ssRNA(+) into the cell, SFV and other related species are different in that up to 20% of released viral particles already contains dsDNA genomes. This is due to a unique feature of spumaviruses in which the onset of reverse transcription of genomic RNA occurs before release rather than after entry of the new host cell like in other retroviruses. [8]

Replication and transcription

As both ssRNA(+) and dsDNA enter the cell, the remaining ssRNA is copied into dsDNA through reverse transcriptase. Nuclear entry of the viral dsDNA is covalently integrated into the cell's genome by the viral integrase, forming a provirus. The integrated provirus utilizes the promoter elements in the 5'LTR to drive transcription. This gives rise to the unspliced full length mRNA that will serve as genomic RNA to be packaged into virions, or used as a template for translation of gag. [8] The spliced mRNAs encode pol (PR, RT, RnaseH, IN) and env (SU, TM) that will be used to later assemble the viral particles.[ citation needed ]

The Tas trans-activator protein augments transcription directed by the LTR through cis-acting targets in the U3 domain of the LTR. [12] The presence of this protein is crucial, as in the absence of Tas, LTR-mediated transcription cannot be detected. Foamy viruses utilize multiple promoters.The IP is required for viral infectivity in tissue culture, as this promoter has a higher basal transcription level than the LTR promoter, and its use leads to transcripts encoding Tas and Bet. Once levels of Tas accumulate, it begins to make use of the LTR promoter, which binds Tas with lower affinity than the IP and leads to accumulation of gag, pol, and env transcripts. [7]

Assembly and release

The SFV capsid is assembled in the cytoplasm as a result of multimerization of Gag molecules, but unlike other related viruses, SFV Gag lacks an N-terminal myristylation signal and capsids are not targeted to the plasma membrane (PM). They require expression of the envelope protein for budding of intracellular capsids from the cell, suggesting a specific interaction between the Gag and Env proteins. Evidence for this interaction was discovered in 2001 when a deliberate mutation for a conserved arginine (Arg) residue at position 50 to alanine of the SFVcpz inhibited proper capsid assembly and abolished viral budding even in the presence of the envelope glycoproteins. [13] Analysis of the glycoproteins on the envelope of the viral particle indicate that it is localized to the endoplasmic reticulum (ER), and that once it buds from the organelle, the maturation process is finalized and can leave to infect additional cells. A dipeptide of two lysine residues (dilysine) was the identified motif that determined to be the specific molecule that mediated the signal, localizing viral particles in the ER. [14]

Modulation and interaction of host cell

There is little data on how SFV interacts with the host cell as the infection takes its course. The most obvious effect that can be observed is the formation of syncytia that results in multinucleated cells. While the details for how SFV can induce this change are not known, the related HIV does cause similar instances among CD4+ T cells. As the cell transcribes the integrated proviral genome, glycoproteins are produced and displayed at the surface of the cell. If enough proteins are at the surface with other CD4+ T cells nearby, the glycoproteins will attach and result in the fusion of several cells. [15]

Foamy degeneration, or vacuolization is another observable change within the cells, but it is unknown how SFV results in the formation of numerous cytoplasmic vacuoles. This is another characteristic of retroviruses, but there are no studies or explanations on why this occurs. [16]

Transmission and pathogenicity

The transmission of SFV is believed to spread through saliva, because large quantities of viral RNA, indicative of SFV gene expression and replication, are present in cells of the oral mucosa. [1] Aggressive behaviors such as bites, to nurturing ones such as a mother licking an infant all have the ability to spread the virus. [7] Studies of natural transmission suggest that infants of infected mothers are resistant to infection, presumably because of passive immunity from maternal antibodies, but infection becomes detectable by three years of age. [17] Little else is known about the prevalence and transmission patterns of SFV in wild-living primate populations.[ citation needed ]

The first case of a spumavirus being isolated from a primate was in 1955 (Rustigan et al., 1955) from the kidneys. [18] What is curious about the cytopathology of SFV is that while it results in rapid cell death for cells in vitro, it loses its highly cytopathic nature in vivo. [7] With little evidence to suggest that SFV infection causes illness, some scientists believe that it has a commensal relationship to simians. [19]

In one study to determine the effects of SFV(mac239) on rhesus macaques that were previously infected with another type of the virus, the experiment had provided evidence that previous infection can increase the risk viral loads reaching unsustainable levels, killing CD4+ T cells and ultimately resulting in the expiration of the doubly infected subjects. SFV/SIV models have since been proposed to replicate the relationship between SFV and HIV in humans, a potential health concern for officials. [20]

Tropism

SFV can infect a wide range of cells, with in vitro experiments confirming that fibroblasts, epithelial cells, and neural cells all showed extensive cytopathology that is characteristic of foamy virus infection. The cytopathic effects in B lymphoid cells and macrophages was reduced, where reverse transcriptase values were lower when compared to fibroblasts and epithelial cells. Cells that expressed no signs of cytopathy from SFV were the Jurkat and Hut-78 T-cell lines. [21]

Cospeciation of SFV and primates

Phylogenic Tree reveals distant relationship of spumaviruses to other retroviridae. Phylogeny of Retroviruses.svg
Phylogenic Tree reveals distant relationship of spumaviruses to other retroviridae.

The phylogenetic tree analysis of SFV polymerase and mitochondrial cytochrome oxidase subunit II (COII has been shown as a powerful marker used for primate phylogeny) from African and Asian monkeys and apes provides very similar branching order and divergence times among the two trees, supporting the cospeciation. Also, the substitution rate in the SFV gene was found to be extremely slow, i.e. the SFV has evolved at a very low rate (1.7×10−8 substitutions per site per year). These results suggest SFV has been cospeciated with Old World primates for about 30 million years, making them the oldest known vertebrate RNA viruses. [19]

The SFV sequence examination of species and subspecies within each clade of the phylogenetic tree of the primates indicated cospeciation of SFV and the primate hosts, as well. A strong linear relationship was found between the branch lengths for the host and SFV gene trees, which indicated synchronous genetic divergence in both data sets. [19]

By using the molecular clock, it was observed that the substitution rates for the host and SFV genes were very similar. The substitution rates for host COII gene and the SFV gene were found out to be (1.16±0.35)×10−8 and (1.7±0.45)×10−8 respectively. This is the slowest rate of substitution observed for RNA viruses and is closer to that of DNA viruses and endogenous retroviruses. This rate is quite different from that of exogenous RNA viruses such as HIV and influenza A virus (10−3 to 10−4 substitutions per site per year). [19]

Prevalence

Researchers in Cameroon, the Democratic Republic of the Congo, France, Gabon, Germany, Japan, Rwanda, the United Kingdom, and the United States have found that simian foamy virus is widespread among wild chimpanzees throughout equatorial Africa. [1]

Humans exposed to wild primates, including chimpanzees, can acquire SFV infections. [2] [22] Since the long-term consequences of these cross-species infections are not known, it is important to determine to what extent wild primates are infected with simian foamy viruses. In this study, researchers tested this question for wild chimpanzees by using novel noninvasive methods. Analyzing over 700 fecal samples from 25 chimpanzee communities across sub-Saharan Africa, the researchers obtained viral sequences from a large proportion of these communities, showing a range of infection rates from 44% to 100%.[ citation needed ]

Major disease outbreaks have originated from cross-species transmission of infectious agents between primates and humans, making it important to learn more about how these cross-species transfers occur. The high SFV infection rates of chimpanzees provide an opportunity to monitor where humans are exposed to these viruses. Identifying the locations may help determine where the highest rates of human–chimpanzee interactions occur. This may predict what other pathogens may jump the species barrier next.[ citation needed ]

Related Research Articles

<span class="mw-page-title-main">Retrovirus</span> Family of viruses

A retrovirus is a type of virus that inserts a DNA copy of its RNA genome into the DNA of a host cell that it invades, thus changing the genome of that cell. After invading a host cell's cytoplasm, the virus uses its own reverse transcriptase enzyme to produce DNA from its RNA genome, the reverse of the usual pattern, thus retro (backwards). The new DNA is then incorporated into the host cell genome by an integrase enzyme, at which point the retroviral DNA is referred to as a provirus. The host cell then treats the viral DNA as part of its own genome, transcribing and translating the viral genes along with the cell's own genes, producing the proteins required to assemble new copies of the virus. Many retroviruses cause serious diseases in humans, other mammals, and birds.

<i>Simian immunodeficiency virus</i> Species of retrovirus

Simian immunodeficiency virus (SIV) is a species of retrovirus that cause persistent infections in at least 45 species of non-human primates. Based on analysis of strains found in four species of monkeys from Bioko Island, which was isolated from the mainland by rising sea levels about 11,000 years ago, it has been concluded that SIV has been present in monkeys and apes for at least 32,000 years, and probably much longer.

<i>Gammaretrovirus</i> Genus of viruses

Gammaretrovirus is a genus in the Retroviridae family. Example species are the murine leukemia virus and the feline leukemia virus. They cause various sarcomas, leukemias and immune deficiencies in mammals, reptiles and birds.

<span class="mw-page-title-main">Endogenous retrovirus</span> Inherited retrovirus encoded in an organisms genome

Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome.

<i>Jaagsiekte sheep retrovirus</i> Species of virus

Jaagsiekte sheep retrovirus (JSRV) is a betaretrovirus which is the causative agent of a contagious lung cancer in sheep, called ovine pulmonary adenocarcinoma.

Rous sarcoma virus (RSV) is a retrovirus and is the first oncovirus to have been described. It causes sarcoma in chickens.

The murine leukemia viruses are retroviruses named for their ability to cause cancer in murine (mouse) hosts. Some MLVs may infect other vertebrates. MLVs include both exogenous and endogenous viruses. Replicating MLVs have a positive sense, single-stranded RNA (ssRNA) genome that replicates through a DNA intermediate via the process of reverse transcription.

Human foamy virus (HFV) is a retrovirus and specifically belongs to the genus Spumavirus. The spumaviruses are complex and significantly different from the other six genera of retroviruses in several ways. The foamy viruses derive their name from the characteristic ‘foamy’ appearance of the cytopathic effect (CPE) induced in the cells. Foamy virus in humans occurs only as a result of zoonotic infection.

Group-specific antigen, or gag, is the polyprotein that contains the core structural proteins of an Ortervirus. It was named as such because scientists used to believe it was antigenic. Now it is known that it makes up the inner shell, not the envelope exposed outside. It makes up all the structural units of viral conformation and provides supportive framework for mature virion.

Env is a viral gene that encodes the protein forming the viral envelope. The expression of the env gene enables retroviruses to target and attach to specific cell types, and to infiltrate the target cell membrane.

Visna-maedi virus from the genus Lentivirus and subfamily Orthoretrovirinae, is a retrovirus that causes encephalitis and chronic pneumonitis in sheep. It is known as visna when found in the brain, and maedi when infecting the lungs. Lifelong, persistent infections in sheep occur in the lungs, lymph nodes, spleen, joints, central nervous system, and mammary glands; The condition is sometimes known as ovine progressive pneumonia (OPP), particularly in the United States, or Montana sheep disease. White blood cells of the monocyte/macrophage lineage are the main target of the virus.

<span class="mw-page-title-main">Syncytin-1</span> Protein-coding gene in the species Homo sapiens

Syncytin-1 also known as enverin is a protein found in humans and other primates that is encoded by the ERVW-1 gene. Syncytin-1 is a cell-cell fusion protein whose function is best characterized in placental development. The placenta in turn aids in embryo attachment to the uterus and establishment of a nutrient supply.

Avian sarcoma leukosis virus (ASLV) is an endogenous retrovirus that infects and can lead to cancer in chickens; experimentally it can infect other species of birds and mammals. ASLV replicates in chicken embryo fibroblasts, the cells that contribute to the formation of connective tissues. Different forms of the disease exist, including lymphoblastic, erythroblastic, and osteopetrotic.

Bovine immunodeficiency virus (BIV) is a retrovirus belonging to the genus Lentivirus. It is similar to the human immunodeficiency virus (HIV) and infects cattle. The cells primarily infected are lymphocytes and monocytes/macrophages.

Mason-Pfizer monkey virus (M-PMV), formerly Simian retrovirus (SRV), is a species of retroviruses that usually infect and cause a fatal immune deficiency in Asian macaques. The ssRNA virus appears sporadically in mammary carcinoma of captive macaques at breeding facilities which expected as the natural host, but the prevalence of this virus in feral macaques remains unknown. M-PMV was transmitted naturally by virus-containing body fluids, via biting, scratching, grooming, and fighting. Cross contaminated instruments or equipment (fomite) can also spread this virus among animals.

Human Endogenous Retrovirus-W (HERV-W) is a family of Human Endogenous Retroviruses, or HERVs.

Bovine foamy virus (BFV) is a ss(+)RNA retrovirus that belongs to the genus spumaviridae. Spumaviruses differ from the other six members of family retroviridae, both structurally and in pathogenic nature. Spumaviruses derive their name from spuma the latin for "foam". The 'foam' aspect of 'foamy virus' comes from syncytium formation and the rapid vacuolization of infected cells, creating a 'foamy' appearance.

Feline foamy virus or Feline syncytial virus is a retrovirus and belongs to the family Retroviridae and the subfamily Spumaretrovirinae. It shares the genus Felispumavirus with only Puma feline foamy virus. There has been controversy on whether FeFV is nonpathogenic as the virus is generally asymptomatic in affected cats and does not cause disease. However, some changes in kidney and lung tissue have been observed over time in cats affected with FeFV, which may or may not be directly affiliated. This virus is fairly common and infection rates gradually increase with a cat's age. Study results from antibody examinations and PCR analysis have shown that over 70% of felines over 9 years old were seropositive for Feline foamy virus. Viral infections are similar between male and female domesticated cats whereas in the wild, more feral females cats are affected with FeFV.

Equine foamy virus (EFV), also called foamy virus (FV), is virus in the genus Equispumavirus. It shares similarities, with respect to replication, with lentiviruses. EFV, along with other FVs are from the family Retroviridae and subfamily Spumaretrovirinae. Spumarivuses, such as EFV, are complicated retroviruses that have been characterized in many animals including nonhuman primates, cattle, cats. Additionally, these viruses have been identified in animals that most often carry lentiviruses.

<span class="mw-page-title-main">Enzootic nasal tumor virus</span> Species of virus

The enzootic nasal tumor virus of the betaretrovirus genus is a carcinogenic retrovirus that causes enzootic nasal adenocarcinoma in sheep and goats. Strain ENTV-1 is found in sheep and strain ENTV-2 is found in goats. The virus causes tumor growth in the upper nasal cavity and is closely related to JSRV which also causes respiratory tumors in ovine. The disease, enzootic nasal adenocarcinoma is common in North America and is found in sheep and goats on every continent except New Zealand and Australia. There are more than 27 betaretroviruses similar to ENVT and JSRV in the ovine genome. In the future, research on ENTV may become important in studying viruses that cause human lung cancer.

References

  1. 1 2 3 4 Liu W, Worobey M, Li Y, Keele BF, Bibollet-Ruche F, Guo Y, et al. (July 2008). "Molecular ecology and natural history of simian foamy virus infection in wild-living chimpanzees". PLOS Pathogens. 4 (7): e1000097. doi: 10.1371/journal.ppat.1000097 . PMC   2435277 . PMID   18604273.
  2. 1 2 Wolfe ND, Switzer WM, Carr JK, Bhullar VB, Shanmugam V, Tamoufe U, et al. (March 2004). "Naturally acquired simian retrovirus infections in central African hunters". Lancet. 363 (9413): 932–937. doi:10.1016/S0140-6736(04)15787-5. PMID   15043960. S2CID   17264100.
  3. ↑ Specter M (20 December 2010). "The Doomsday Strain". Letter from Cameroon. New Yorker.
  4. 1 2 Plochmann K, Horn A, Gschmack E, Armbruster N, Krieg J, Wiktorowicz T, et al. (September 2012). "Heparan sulfate is an attachment factor for foamy virus entry". Journal of Virology. 86 (18): 10028–10035. doi:10.1128/JVI.00051-12. PMC   3446549 . PMID   22787203.
  5. ↑ Coffin J (1992). Structure and Classification of Retroviruses. New York: Plenum Press. p. 20. ISBN   978-0-306-44074-8.
  6. ↑ Sakai K, Ami Y, Suzaki Y, Matano T (December 2016). "First Complete Genome Sequence of a Simian Foamy Virus Isolate from a Cynomolgus Macaque". Genome Announcements. 4 (6): e01332–16. doi:10.1128/genomeA.01332-16. PMC   5137406 . PMID   27908992.
  7. 1 2 3 4 5 Linial ML (March 1999). "Foamy viruses are unconventional retroviruses". Journal of Virology. 73 (3): 1747–1755. doi:10.1128/JVI.73.3.1747-1755.1999. PMC   104413 . PMID   9971751.
  8. 1 2 3 "Spumavirus". viralzone.expasy.org. SIB Swiss Institute of Bioinformatics. Retrieved 2017-11-02.
  9. ↑ "bet - Protein Bet - Simian foamy virus (isolate chimpanzee) (SFVcpz) - bet gene & protein". www.uniprot.org. Retrieved 2017-11-03.
  10. ↑ Picard-Maureau M, Jarmy G, Berg A, Rethwilm A, Lindemann D (April 2003). "Foamy virus envelope glycoprotein-mediated entry involves a pH-dependent fusion process". Journal of Virology. 77 (8): 4722–4730. doi:10.1128/JVI.77.8.4722-4730.2003. PMC   152125 . PMID   12663779.
  11. ↑ Lehmann-Che J, Giron ML, Delelis O, Löchelt M, Bittoun P, Tobaly-Tapiero J, et al. (July 2005). "Protease-dependent uncoating of a complex retrovirus". Journal of Virology. 79 (14): 9244–9253. doi:10.1128/JVI.79.14.9244-9253.2005. PMC   1168774 . PMID   15994819.
  12. ↑ Campbell M, Renshaw-Gegg L, Renne R, Luciw PA (August 1994). "Characterization of the internal promoter of simian foamy viruses". Journal of Virology. 68 (8): 4811–4820. doi:10.1128/JVI.68.8.4811-4820.1994. PMC   236420 . PMID   8035481.
  13. ↑ Eastman SW, Linial ML (August 2001). "Identification of a conserved residue of foamy virus Gag required for intracellular capsid assembly". Journal of Virology. 75 (15): 6857–6864. doi:10.1128/JVI.75.15.6857-6864.2001. PMC   114413 . PMID   11435565.
  14. ↑ Goepfert PA, Shaw KL, Ritter GD, Mulligan MJ (January 1997). "A sorting motif localizes the foamy virus glycoprotein to the endoplasmic reticulum". Journal of Virology. 71 (1): 778–784. doi:10.1128/JVI.71.1.778-784.1997. PMC   191117 . PMID   8985416.
  15. ↑ Huerta L, López-Balderas N, Rivera-Toledo E, Sandoval G, Gómez-Icazbalceta G, Villarreal C, et al. (August 2009). "HIV-envelope-dependent cell-cell fusion: quantitative studies". TheScientificWorldJournal. 9: 746–763. doi: 10.1100/tsw.2009.90 . PMC   5823155 . PMID   19705036.
  16. ↑ "Cytopathic Effects of Viruses Protocols". 2012-06-02. Archived from the original on 2012-06-02. Retrieved 2017-11-03.{{cite web}}: CS1 maint: bot: original URL status unknown (link)
  17. ↑ Stenbak CR, Craig KL, Ivanov SB, Wang X, Soliven KC, Jackson DL, et al. (January 2014). "New World simian foamy virus infections in vivo and in vitro". Journal of Virology. 88 (2): 982–991. doi:10.1128/JVI.03154-13. PMC   3911628 . PMID   24198412.
  18. ↑ Loh PC (1993). "Spumaviruses". The Retroviridae. The Viruses. Springer, Boston, MA. pp. 361–397. doi:10.1007/978-1-4899-1627-3_6. ISBN   9781489916297.
  19. 1 2 3 4 Switzer WM, Salemi M, Shanmugam V, Gao F, Cong ME, Kuiken C, et al. (March 2005). "Ancient co-speciation of simian foamy viruses and primates". Nature. 434 (7031): 376–380. Bibcode:2005Natur.434..376S. doi:10.1038/nature03341. PMID   15772660. S2CID   4326578.
  20. ↑ Choudhary A, Galvin TA, Williams DK, Beren J, Bryant MA, Khan AS (June 2013). "Influence of naturally occurring simian foamy viruses (SFVs) on SIV disease progression in the rhesus macaque (Macaca mulatta) model". Viruses. 5 (6): 1414–1430. doi: 10.3390/v5061414 . PMC   3717714 . PMID   23744104.
  21. ↑ Mergia A, Leung NJ, Blackwell J (January 1996). "Cell tropism of the simian foamy virus type 1 (SFV-1)". Journal of Medical Primatology. 25 (1): 2–7. doi:10.1111/j.1600-0684.1996.tb00185.x. PMID   8740945. S2CID   35148544.
  22. ↑ Switzer WM, Bhullar V, Shanmugam V, Cong ME, Parekh B, Lerche NW, et al. (March 2004). "Frequent simian foamy virus infection in persons occupationally exposed to nonhuman primates". Journal of Virology. 78 (6): 2780–2789. doi:10.1128/JVI.78.6.2780-2789.2004. PMC   353775 . PMID   14990698.
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