An Andersen sampler or Andersen impactor is a cascade impactor used to determine the amount of viable pathogens in a given area, in particular bacteria and fungi. Unlike real-time electronic particle counters, the Andersen sampler imparts pathogens on petri dishes, which require incubation. Thus, calculation of the contaminated air requires working backwards from the resulting pathogen growth in each dish.
Andersen samplers have been used to assess the nature of pathogenic aerosols in various scientific papers. It can also be used to study inert aerosols, when each stage of the sampler is used to model respiratory particle deposition.
Andersen's paper from 1958 describes a six-stage Andersen sampler that counts "viable airborne particles". In each stage, air goes through specifically sized holes, past a petri dish that aerosols impact, before subsequently moving through smaller holes towards the other petri dishes. Each stage gradually increases the velocity of the air; the sampler relies on inertia for particles to leave the air stream. [1]
Calculation of the number of viable particles is normally done by counting colonies via a microscope, called the "microscope method" in Andersen's paper. For stages 3 through 6, Andersen provides an alternate means of calculating viable particles via the "positive hole method". The method involves counting the number of colonies from a macroscopic point of view; each visible colony corresponds to a hole in each stage of the Andersen sampler. A conversion table is then used to quickly calculate the number of viable particles. [1]
Andersen samplers have been used in various places and industries, including (but not limited to), compost facilities, to help mitigate the health impacts of fungal spores, [2] agriculture, to track bioaerosols more generally from livestock, [3] textiles, [4] apartment buildings, [5] wastewater facilities, [6] and even the aerosolization of anthrax following attacks in 2001. [7] Samplers have also been used to study inert lead dust, with each stage designed to simulate the deposition characteristics of the respiratory tract. [8]
Information on the development of the Andersen sampler was initially classified by the US army, but is declassified as of January 24, 1958. [9] A noted early use of the Andersen sampler was the tracking of disease in the states of Oregon and California. [10]
Continued development of the Andersen sampler was then the focus of Andersen 2000, Inc., originally CMC Industries, which had renamed itself following the acquisition of the relevant patents. As of November 1970, the company had released four models of the Andersen sampler, each targeted towards different industries, with one being handheld. However, the stack sampler continued to be the company's most popular model. [11]
An assessment of the Andersen Mark-II cascade impactor was published in 1988. [12]
In 2012, a comparison was made between the "culturable particles" (CP) method and the "culturable organisms" (CO) method with the help of an Aerodynamic Particle Sizer (APS). The distributions between the CP and CO method were different enough for the null hypothesis to be rejected; the authors suggest that this may be due to the higher resolution particle count afforded by the APS, the Andersen sampler's wall losses, or the unintended collection of smaller particles in the earlier stages. [13]
In 2013, a group of researchers improved the collection efficiency of the Andersen sampler by adding mineral oil to the petri dish agar. Results were supported through the use of an optical particle counter. [14]
An aerosol is a suspension of fine solid particles or liquid droplets in air or another gas. Aerosols can be generated from natural or human causes. The term aerosol commonly refers to the mixture of particulates in air, and not to the particulate matter alone. Examples of natural aerosols are fog, mist or dust. Examples of human caused aerosols include particulate air pollutants, mist from the discharge at hydroelectric dams, irrigation mist, perfume from atomizers, smoke, dust, sprayed pesticides, and medical treatments for respiratory illnesses.
Aerobiology is a branch of biology that studies the passive transport of organic particles, such as bacteria, fungal spores, very small insects, pollen grains and viruses. Aerobiologists have traditionally been involved in the measurement and reporting of airborne pollen and fungal spores as a service to those with allergies. However, aerobiology is a varied field, relating to environmental science, plant science, meteorology, phenology, and climate change.
In medicine, public health, and biology, transmission is the passing of a pathogen causing communicable disease from an infected host individual or group to a particular individual or group, regardless of whether the other individual was previously infected. The term strictly refers to the transmission of microorganisms directly from one individual to another by one or more of the following means:
A surgical mask, also known by other names such as a medical face mask or procedure mask, is a personal protective equipment used by healthcare professionals that serves as a mechanical barrier that interferes with direct airflow in and out of respiratory orifices. This helps reduce airborne transmission of pathogens and other aerosolized contaminants between the wearer and nearby people via respiratory droplets ejected when sneezing, coughing, forceful expiration or unintentionally spitting when talking, etc. Surgical masks may be labeled as surgical, isolation, dental or medical procedure masks.
Aeroplankton are tiny lifeforms that float and drift in the air, carried by wind. Most of the living things that make up aeroplankton are very small to microscopic in size, and many can be difficult to identify because of their tiny size. Scientists collect them for study in traps and sweep nets from aircraft, kites or balloons. The study of the dispersion of these particles is called aerobiology.
Aerosolization is the process or act of converting some physical substance into the form of particles small and light enough to be carried on the air i.e. into an aerosol. Aerosolization refers to a process of intentionally oxidatively converting and suspending particles or a composition in a moving stream of air for the purpose of delivering the oxidized particles or composition to a particular location.
Bioaerosols are a subcategory of particles released from terrestrial and marine ecosystems into the atmosphere. They consist of both living and non-living components, such as fungi, pollen, bacteria and viruses. Common sources of bioaerosols include soil, water, and sewage.
Airborne transmission or aerosol transmission is transmission of an infectious disease through small particles suspended in the air. Infectious diseases capable of airborne transmission include many of considerable importance both in human and veterinary medicine. The relevant infectious agent may be viruses, bacteria, or fungi, and they may be spread through breathing, talking, coughing, sneezing, raising of dust, spraying of liquids, flushing toilets, or any activities which generate aerosol particles or droplets.
Indoor bioaerosol is bioaerosol in an indoor environment. Bioaerosols are natural or artificial particles of biological origin suspended in the air. These particles are also referred to as organic dust. Bioaerosols may consist of bacteria, fungi, viruses, microbial toxins, pollen, plant fibers, etc. Size of bioaerosol particles varies from below 1 μm to 100 μm in aerodynamic diameter; viable bioaerosol particles can be suspended in air as single cells or aggregates of microorganism as small as 1–10 μm in size. Since bioaerosols are potentially related to various human health effects and the indoor environment provides a unique exposure situation, concerns about indoor bioaerosols have increased over the last decade.
Aerosol mass spectrometry is the application of mass spectrometry to the analysis of the composition of aerosol particles. Aerosol particles are defined as solid and liquid particles suspended in a gas (air), with size range of 3 nm to 100 μm in diameter and are produced from natural and anthropogenic sources, through a variety of different processes that include wind-blown suspension and combustion of fossil fuels and biomass. Analysis of these particles is important owing to their major impacts on global climate change, visibility, regional air pollution and human health. Aerosols are very complex in structure, can contain thousands of different chemical compounds within a single particle, and need to be analysed for both size and chemical composition, in real-time or off-line applications.
A respiratory droplet is a small aqueous droplet produced by exhalation, consisting of saliva or mucus and other matter derived from respiratory tract surfaces. Respiratory droplets are produced naturally as a result of breathing, speaking, sneezing, coughing, or vomiting, so they are always present in our breath, but speaking and coughing increase their number.
Dustiness may be defined as the propensity of a finely divided solid to form an airborne dust (aerosol) from a mechanical or aerodynamic stimulus. Dustiness can be influenced by particle morphology (shape), size, and inter-particle forces. Dustiness increases the risk of inhalation exposure.
A cascade impactor measures the reach range of a particulate substance as it moves through an opening with the use of aerosol. Cascade impactors are strictly measurement-related devices. In addition to measuring the range of substances moved through an opening by aerosol, the impactor can also be used to determine the particle size of the distributed substance. A cascade impactor collects its samples in a graduated manner. This allows the user to identify the sizes of the substance particles as the particles are distributed from the propellant aerosol source. When the aerosol substance is distributed into the cascade impactor, the substance enters a series of discs designed to collect solids and different particulate matter. The substance is thus collected as it passes through the disc series. Each disc is set in sequence with both the prior and the previous disc. The size of the discs is graduated as well, to properly determine the size of the particulate matter at each stage of the impactor.
A toilet plume is the cloud like dispersal of potentially infectious microscopic sewage particles & water vapor as a result of flushing a toilet. Day to day use of a toilet by healthy individuals is considered to be of a lower health risk. However this dynamic completely changes if an individual is fighting an illness and currently shedding out large quantities of an infectious virulent pathogen in their urine, feces or vomitus. There is evidence that specific pathogens such as norovirus or SARS coronavirus could potentially be spread by toilet aerosols, but as of 2015 no direct experimental studies had refuted actual disease transmission from toilet aerosols. It has been hypothesized that dispersal of pathogens may be reduced by closing the toilet lid before flushing, and by using toilets with lower flush energy. 2024 Science empirically built on to this theory, by illustrating that the viruses that toilet plume contains still spreads out the gaps in the seat onto the walls and concentrating on the surrounding floors.
Kimberly A. Prather is an American atmospheric chemist. She is a distinguished chair in atmospheric chemistry and a distinguished professor at the Scripps Institution of Oceanography and department of chemistry and biochemistry at UC San Diego. Her work focuses on how humans are influencing the atmosphere and climate. In 2019, she was elected a member of the National Academy of Engineering for technologies that transformed understanding of aerosols and their impacts on air quality, climate, and human health. In 2020, she was elected as a member of the National Academy of Sciences. She is also an elected Fellow of the American Philosophical Society, American Geophysical Union, the American Association for the Advancement of Science, American Philosophical Society, and the American Academy of Arts and Sciences.
The North Atlantic Aerosols and Marine Ecosystems Study (NAAMES) was a five-year scientific research program that investigated aspects of phytoplankton dynamics in ocean ecosystems, and how such dynamics influence atmospheric aerosols, clouds, and climate. The study focused on the sub-arctic region of the North Atlantic Ocean, which is the site of one of Earth's largest recurring phytoplankton blooms. The long history of research in this location, as well as relative ease of accessibility, made the North Atlantic an ideal location to test prevailing scientific hypotheses in an effort to better understand the role of phytoplankton aerosol emissions on Earth's energy budget.
A dental aerosol is an aerosol that is produced from dental instrument, dental handpieces, three-way syringes, and other high-speed instruments. These aerosols may remain suspended in the clinical environment. Dental aerosols can pose risks to the clinician, staff, and other patients. The heavier particles contained within the aerosols are likely to remain suspended in the air for relatively short period and settle quickly onto surfaces, however, the lighter particles may remain suspended for longer periods and may travel some distance from the source. These smaller particles are capable of becoming deposited in the lungs when inhaled and provide a route of diseases transmission. Different dental instruments produce varying quantities of aerosol, and therefore are likely to pose differing risks of dispersing microbes from the mouth. Air turbine dental handpieces generally produce more aerosol, with electric micromotor handpieces producing less, although this depends on the configuration of water coolant used by the handpiece.
Source control is a strategy for reducing disease transmission by blocking respiratory secretions produced through breathing, speaking, coughing, sneezing or singing. Multiple source control techniques can be used in hospitals, but for the general public wearing personal protective equipment during epidemics or pandemics, respirators provide the greatest source control, followed by surgical masks, with cloth face masks recommended for use by the public only when there are shortages of both respirators and surgical masks.
The transmission of COVID-19 is the passing of coronavirus disease 2019 from person to person. COVID-19 is mainly transmitted when people breathe in air contaminated by droplets/aerosols and small airborne particles containing the virus. Infected people exhale those particles as they breathe, talk, cough, sneeze, or sing. Transmission is more likely the closer people are. However, infection can occur over longer distances, particularly indoors.
Workplace exposure monitoring is the monitoring of substances in a workplace that are chemical or biological hazards. It is performed in the context of workplace exposure assessment and risk assessment. Exposure monitoring analyzes hazardous substances in the air or on surfaces of a workplace, and is complementary to biomonitoring, which instead analyzes toxicants or their effects within workers.