Apifresh

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Apifresh [1] is a European project funded by the European Commission 7th Framework Program. [2] It started on 1 July 2010 and it will last three years. The project is developed by a Consortium set up by partners from different European Countries. It is formed by four Industrial Associations, three SMEs and three research centres.

Contents

Apifresh project has come out in several media:

Rubus flower Rubus flower.jpg
Rubus flower

The partners

The coordinator is the research centre Tecnologías Avanzadas Inspiralia. The Industrial Associations are: European Professional Beekeepers Association; Orszagos Magyar Meheszeti Egyesulet; Federecao Nacional dos Apicultores de Portugal and Centro Tecnológico Nacional de la Conserva y Alimentación. The three SMEs are: Campomiel from Spain; Balparmak from Turkey and Parco Tecnologico Padano from Italy. The research centres are Tecnologías Avanzadas Inspiralia (coordinator); Centro Agrícola Regional de Marchamalo, Universidad Complutense de Madrid and TÜBITAK MAM, Gida Enstitüsü.

Objectives

The objective of the Apifresh project is to provide European beekeepers with the scientific and technological aids necessary to improve the quality of European pollen and royal jelly and also to promote the regulatory means that will allow European bee products compete under fair conditions against lower quality or adulterated products.

Pollen samples Pollen samples.jpg
Pollen samples

These core objectives will be realised by means of the following achievements:

  1. The development of a quality standard aimed at protecting European pollen and royal jelly products against adulteration and contamination induced by environmental and bee-treatment toxic components [9] [10]
  2. The development of the knowledge and technological aids necessary to enforce compliance with the proposed standard, including:
    • Definition of the analytical methods necessary to determine sensory properties, bacterial load, water content, chemical composition, and presence of pesticides and heavy metals. [11] [ unreliable source? ] [12] [13] [14]
    • Determination of bee-product authenticity and unambiguous origin identification [15] [16] by means of PCR techniques
    • Determination of the health enhancing compounds present in bee products. [17] [ unreliable source? ] [18]
    • Development of a set of best practice guidelines for beekeep[ citation needed ]
    • Development of a low-cost computer vision based decision support system for the identification of pollen vegetal species, relative proportions and geographical origin. [19] [20] [21]
Kinds of pollen Kinds of pollen.jpg
Kinds of pollen

The development of best practice guidelines aimed at improving the quality of pollen and royal jelly products in all phases of the production chain: harvesting, collecting, storage, transportation and presentation at the point of sale.

Need

The competitiveness of the European beekeeping sector is progressively falling as a consequence of decreased bee populations and abrupt snatching of market share by imports. [22] These facts cause reduction of beekeepers’ production, implying underutilised resources, lower economies of scale, and higher production costs. [23] In addition, products manufactured in countries with much lower quality standards and/or adulterated with substitutes are gaining market share through unfair competition.

Lack of specific regulations with respect to bee products are another threat for the beekeeping market and public health safety. Currently, there are no accepted standards for certain products like pollen and royal jelly. Few countries have guidelines or standards for products other than honey. This means it is possible to find products in the market under these labels without any quality and authenticity control.

Project status

Apifresh is in the second year of development. Currently the project has collected more than 400 pollen and 20 royal jelly samples from ten European countries. These samples have been analyzed in the two laboratories participating in the project: Marchamalo (Spain) and Tubitak (Turkey) with a third reference laboratory in Germany. A set of guidelines have been produced for the beekeepers to ensure samples are collected according to a given protocol; ensuring the quality of harvesting, transportation and storage conditions. Also strict interlaboratory validation protocols have been defined; including: a validation protocol, a validation master plan and standard format for inter-laboratory reports.[ citation needed ]

A complete set of analytics has been specified in the protocol; including the following:[ citation needed ]

These analyses have been performed already in the set of available samples and the results from the 2011 sample collection campaign area available. Validation for the chemical analysis has been performed following Eurochem /Citac Guide GC 4 (QUAM:2000.1, second edition) while validation studies for microbiological analysis has been done based on ISO 19036.

In addition the first computer vision software module has been completed and is already available. The module is able to separate pollen samples by its color, categorize them according to an internal dictionary of pollen species and count the relative proportions of the different pollen types in the sample. All this is done automatically in a few seconds, using a low cost video camera and PC computer. No expertise in computer vision or Melissopalynology is required. Before, this same process required at least two hours work by a skilled operator separating pollen grains by hand. As reported in scientific publications, [24] the software achieves 94% accuracy in the identification of the pollen proportions; much higher than what is reasonably possible with a human operator. [25]

Related Research Articles

<span class="mw-page-title-main">Bee</span> Clade of insects

Bees are winged insects closely related to wasps and ants, known for their roles in pollination and, in the case of the best-known bee species, the western honey bee, for producing honey. Bees are a monophyletic lineage within the superfamily Apoidea. They are currently considered a clade, called Anthophila. There are over 20,000 known species of bees in seven recognized biological families. Some species – including honey bees, bumblebees, and stingless bees – live socially in colonies while most species (>90%) – including mason bees, carpenter bees, leafcutter bees, and sweat bees – are solitary.

<span class="mw-page-title-main">Honey</span> Sweet and viscous substance made by bees mostly using nectar from flowers

Honey is a sweet and viscous substance made by several species of bees, the best-known of which are honey bees. Honey is made and stored to nourish bee colonies. Bees produce honey by gathering and then refining the sugary secretions of plants or the secretions of other insects, like the honeydew of aphids. This refinement takes place both within individual bees, through regurgitation and enzymatic activity, and during storage in the hive, through water evaporation that concentrates the honey's sugars until it is thick and viscous.

<span class="mw-page-title-main">Beeswax</span> Natural wax produced by honey bees of the genus Apis

Beeswax is a natural wax produced by honey bees of the genus Apis. The wax is formed into scales by eight wax-producing glands in the abdominal segments of worker bees, which discard it in or at the hive. The hive workers collect and use it to form cells for honey storage and larval and pupal protection within the beehive. Chemically, beeswax consists mainly of esters of fatty acids and various long-chain alcohols.

<span class="mw-page-title-main">Honey bee</span> Colonial flying insect of genus Apis

A honey bee is a eusocial flying insect within the genus Apis of the bee clade, all native to mainland Afro-Eurasia. After bees spread naturally throughout Africa and Eurasia, humans became responsible for the current cosmopolitan distribution of honey bees, introducing multiple subspecies into South America, North America, and Australia.

<span class="mw-page-title-main">Forensic palynology</span> Forensic application of the study of particulate matter

Forensic palynology is a subdiscipline of palynology, that aims to prove or disprove a relationship among objects, people, and places that may pertain to both criminal and civil cases. Pollen can reveal where a person or object has been, because regions of the world, countries, and even different parts of a single garden will have a distinctive pollen assemblage. Pollen evidence can also reveal the season in which a particular object picked up the pollen. Recent research into forensic palynology has seen advancements in DNA barcoding from pollen, to the level of singular pollen molecules, allowing DNA profiles to be created from singular palynomorphs, streamlining the efficiency and accuracy of taxonomic identification.

<span class="mw-page-title-main">Beekeeper</span> Person who keeps honey bees

A beekeeper is a person who keeps honey bees, a profession known as beekeeping.

<span class="mw-page-title-main">Bee brood</span> Chamber of a beehive

In beekeeping, bee brood or brood refers to the eggs, larvae and pupae of honeybees. The brood of Western honey bees develops within a bee hive. In man-made, removable frame hives, such as Langstroth hives, each frame which is mainly occupied by brood is called a brood frame. Brood frames usually have some pollen and nectar or honey in the upper corners of the frame. The rest of the brood frame cells may be empty or occupied by brood in various developmental stages. During the brood raising season, the bees may reuse the cells from which brood has emerged for additional brood or convert it to honey or pollen storage. Bees show remarkable flexibility in adapting cells to a use best suited for the hive's survival.

<span class="mw-page-title-main">Queen bee</span> Egg-laying individual in a bee colony

A queen bee is typically an adult, mated female (gyne) that lives in a colony or hive of honey bees. With fully developed reproductive organs, the queen is usually the mother of most, if not all, of the bees in the beehive. Queens are developed from larvae selected by worker bees and specially fed in order to become sexually mature. There is normally only one adult, mated queen in a hive, in which case the bees will usually follow and fiercely protect her.

<span class="mw-page-title-main">Apitherapy</span> Pseudoscientific alternative medical therapy using bee products

Apitherapy is a branch of alternative medicine that uses honey bee products, including honey, pollen, propolis, royal jelly and bee venom. There has been no scientific or clinical evidence for the efficacy or safety of apitherapy treatments.

<span class="mw-page-title-main">Royal jelly</span> Secretion from the glands of nurse bees

Royal jelly is a honey bee secretion that is used in the nutrition of larvae and adult queens. It is secreted from the glands in the hypopharynx of nurse bees, and fed to all larvae in the colony, regardless of sex or caste.

<span class="mw-page-title-main">Bee pollen</span> Ball of pollen gathered by worker honeybees

Bee pollen, also known as bee bread and ambrosia, is a ball or pellet of field-gathered flower pollen packed by worker honeybees, and used as the primary food source for the hive. It consists of simple sugars, protein, minerals and vitamins, fatty acids, and a small percentage of other components. Bee pollen is stored in brood cells, mixed with saliva, and sealed with a drop of honey. Bee pollen is harvested as food for humans and marketed as having various, but yet unproven, health benefits.

Hive management in beekeeping refers to intervention techniques that a beekeeper may perform to ensure hive survival and to maximize hive production. Hive management techniques vary widely depending on the objectives.

Verification and validation are independent procedures that are used together for checking that a product, service, or system meets requirements and specifications and that it fulfills its intended purpose. These are critical components of a quality management system such as ISO 9000. The words "verification" and "validation" are sometimes preceded with "independent", indicating that the verification and validation is to be performed by a disinterested third party. "Independent verification and validation" can be abbreviated as "IV&V".

<span class="mw-page-title-main">Western honey bee</span> European honey bee

The western honey bee or European honey bee is the most common of the 7–12 species of honey bees worldwide. The genus name Apis is Latin for "bee", and mellifera is the Latin for "honey-bearing" or "honey carrying", referring to the species' production of honey.

<span class="mw-page-title-main">Beekeeping in the United States</span> Commercial beekeeping in the United States

Commercial Beekeeping in the United States dates back to the 1860s.

<span class="mw-page-title-main">Beekeeping in New Zealand</span>

Beekeeping in New Zealand is reported to have commenced in 1839 with the importing of two skep hives by Mary Bumby, a missionary. It has since become an established industry as well a hobby activity.

<span class="mw-page-title-main">Colony collapse disorder</span> Aspect of apiculture

Colony collapse disorder (CCD) is an abnormal phenomenon that occurs when the majority of worker bees in a honey bee colony disappear, leaving behind a queen, plenty of food, and a few nurse bees to care for the remaining immature bees. While such disappearances have occurred sporadically throughout the history of apiculture, and have been known by various names, the syndrome was renamed colony collapse disorder in early 2007 in conjunction with a drastic rise in reports of disappearances of western honey bee colonies in North America. Beekeepers in most European countries had observed a similar phenomenon since 1998, especially in Southern and Western Europe; the Northern Ireland Assembly received reports of a decline greater than 50%. The phenomenon became more global when it affected some Asian and African countries as well. From 1990 to 2021, the United Nation’s FAO calculated that the worldwide number of honeybee colonies increased 47%, reaching 102 million.

Beekeeping in the United Kingdom is the maintenance of bee colonies by humans within the United Kingdom. It is a significant commercial activity that provides those involved with honey, beeswax, royal jelly, queen bees, propolis, flower pollen and bee pollen. Honeybees also provide pollination services to orchards and a variety of seed crops.

<i>Melipona bicolor</i> Species of bee

Melipona bicolorLepeletier, 1836, commonly known as Guaraipo or Guarupu, is a eusocial bee found primarily in South America. It is an inhabitant of the Araucaria Forest and the Atlantic Rainforest, and is most commonly found from South to East Brazil, Bolivia, Argentina, and Paraguay. It prefers to nest close to the soil, in hollowed trunks or roots of trees. M. bicolor is a member of the tribe Meliponini, and is therefore a stingless bee. This species is unique among the stingless bees species because it is polygynous, which is rare for eusocial bees.

References

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  13. Almeida-Muradian, L.B.; Pamplona, Lucila C.; Coimbra, Sı́Lvia; Barth, Ortrud Monika (2005). "Chemical composition and botanical evaluation of dried bee pollen pellets". Journal of Food Composition and Analysis. 18: 105. doi:10.1016/j.jfca.2003.10.008.
  14. Pereira de Melo, Illana Louise; Muradian, Ligia Bicudo de Almeida (2010). "Stability of antioxidants vitamins in bee pollen samples". Química Nova. 33 (3): 514–8. doi: 10.1590/S0100-40422010000300004 .
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