CCDC82

Last updated
CCDC82
Identifiers
Aliases CCDC82 , HSPC048, coiled-coil domain containing 82
External IDs MGI: 1913646; HomoloGene: 11678; GeneCards: CCDC82; OMA:CCDC82 - orthologs
Orthologs
SpeciesHumanMouse
Entrez

79780

66396

Ensembl

ENSG00000149231

ENSMUSG00000079084

UniProt

Q8N4S0

Q6PG04

RefSeq (mRNA)

NM_014148
NM_024725
NM_001318736
NM_001318737
NM_001363594

Contents

NM_025534

RefSeq (protein)

NP_001305665
NP_001305666
NP_079001
NP_001350523

NP_079810

Location (UCSC) Chr 11: 96.35 – 96.39 Mb Chr 9: 13.25 – 13.29 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Coiled-Coil Domain Containing protein 82 (CCDC82) is a protein that in humans, is encoded for by the gene of the same name, CCDC82. The CCDC82 gene is expressed in nearly all of human tissues at somewhat low rates. As of today, there are no patents involving CCDC82 and the function remains unknown.

Gene

CCDC82 is located on chromosome 11 at 11q21.5. [5] It contains two domains of unknown function, DUF4196 and DUF4211. [6] The DNA sequence is 37,155 base pairs long [7] and contains 7 exons. [8]

Homology

CCDC82 is present in many orthologs. It is conserved throughout other mammals, reptiles, birds and bony fish. It is not found in invertebrates, bacteria or fungi. There are no paralogs. [9]

Unrooted phylogenetic tree CCDC82 Unrooted Phylogenetic Tree.png
Unrooted phylogenetic tree
Scientific nameCommon nameDate of divergenceAccession numberLengthPercent identityPercent similarity
Pan troglodytes Chimpanzee 6.3 MyaXP_001147806.1544 aa60.899
Gorilla gorilla gorilla Gorilla 8.8 MyaXP_004052053.1521 aa56.291
Pongo pygmaeus Orangutan 15.7 MyaNP_003253075.1343 aa95.398
Nomascus leucogenys Crested gibbon20.4 MyaXP_003253075.1554 aa57.894
Papio anubis Olive baboon29 MyaXP_003910631.1542 aa5286
Callithrix jacchus Marmoset42.6 MyaXP_002754732.1526 aa51.286
Mus musculus Mouse92.3 MyaNP_079810.2518 aa39.774
Rattus norvegicus Brown rat92.3 MyaNP_001007661.1516 aa37.471
Canis lupus familiaris Dog94.2 MyaXP_542232.2520 aa4679
Bos taurus Cow94.2 MyaNP_001039559.2522 aa42.474
Ailuropoda melanoleuca Giant panda94.2 MyaXP_002925755.1528 aa45.480
Loxodonta africana African bush elephant98.7 MyaXP_003415705.1521 aa40.475
Sarcophilus harrisii Tasmanian devil162.2 MyaXP_003764344.1518 aa36.970
Monodelphis domestica Gray short-tailed opossum162.2 MyaXP_001363143.1516 aa36.772
Ornithorhynchus anatinus Platypus167.4 MyaXP_001511067.1505 aa26.670
Gallus gallus Chicken296 MyaXP_423807.3460 aa24.356
Meleagris gallopavo Wild turkey296 MyaXP_003203546.1462 aa21.570
Taeniopygia guttata Zebra finch296 MyaXP_002198267.1575 aa2061
Anolis carolinensis Carolina anole296 MyaXP_003219357.1603 aa19.949
Xenopus tropicalis Western clawed frog371.2 MyaXP_002935613.1462 aa2173

mRNA

Promoter

The predicted promoter for CCDC82 is located on the minus strand and spans from base pairs 96,122,963 to 96,123,587. It is 625 base pairs long. [10]

Transcription factors

The transcription factors listed below are for the predicted promoter sequence and are located on the minus strand. [11]

Detailed Family InformationSpanScore
Alternative splicing variant of FOXP148-641.00
Homeodomain transcription factor Otx234-50.992
Hypoxia-response Elements111-127.985
Homeobox A10/HOX 1.852-68.957
SRY box 980-104.947
Mesoderm posterior 1 and 242-62.937
c-Myc/Max heterodimer44-60.929
SAM pointed domain containing ets transcription factor27-47.923
cAMP-responsive element binding protein134-154.917
PR domain zinc finger protein 14138-152.912

Protein

The protein it encodes for is 344 amino acids in length. The protein itself is very acidic and is very rich in aspartic acid and glutamic acid. It is also very deficient in alanine, containing only two alanines in the entire sequence. The alanines are located adjacent to each other, amino acid number 233 and 234. Alanine 233 is highly conserved throughout the orthologs. The molecular weight is 40.0 kdal and the isoelectric point is 4.383 [12]

Expression

This image shows the level of expression of CCDC82 in different tissues throughout the body. CCDC82ExpressionData.png
This image shows the level of expression of CCDC82 in different tissues throughout the body.

CCDC82 is found in nearly all tissues in the human body, however it is present in higher quantities in the skeletal muscles, adrenal cortex, and the trigeminal ganglion. [13] [14]

Post translational modifications

CCDC82 has several predicted phosphorylation sites. [15] There are 32 predicted serine phosphorylation sites, 5 threonine, and 3 tyrosine. [16]

Interactions

CCDC82 is known to interact with two proteins. It indirectly interacts with VHL, a gene that encodes for a tumor suppressor and ubiquitin protein ligase. It also interacts with EWSR1, which functions as a transcriptional repressor. [17]

Clinical significance

CCDC82 is a circulat-responsive gene. [18] Circulat is a product designed to restore systemic vascular health. It is a plant based product and taken by patients who suffer from diabetes or circulatory problems. [19]

Possible function

Based on the information that CCDC82 is affected by the Circulat product it could be hypothesized that CCDC82 is involved in circulatory function. However, this is purely speculation.

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000149231 – Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000079084 – Ensembl, May 2017
  3. ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ↑ "CCDC82 Genomic Views". GeneCards. Weizmann Institute of Science.
  6. ↑ "CCDC82 Protein Domains and Families". GeneCards. Weizmann Institute of Science.
  7. ↑ "Homo sapiens chromosome 11, GRCh37.p10 Primary Assembly". NCBI. 13 August 2013.
  8. ↑ "Prediction of several variants of multiple genes". Softberry. Archived from the original on 2013-10-29. Retrieved 2013-05-14.
  9. ↑ "CCDC82 BLAST". BLAST. NCBI.
  10. ↑ "Genome Browser". ElDorado. Genomatix.[ permanent dead link ]
  11. ↑ "Transcription Factor Binding Sites". ElDorado. Genomatix.[ permanent dead link ]
  12. ↑ "SAPS". Biology Workbench. SDSU.[ permanent dead link ]
  13. ↑ "Large-scale analysis of the human transcriptome (HG-U133A)". GeoProfiles. NCBI.
  14. ↑ Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD, Chen JL (August 2000). "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning". Proc. Natl. Acad. Sci. U.S.A. 97 (17): 9543–8. Bibcode:2000PNAS...97.9543H. doi: 10.1073/pnas.160270997 . PMC   16901 . PMID   10931946.
  15. ↑ Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (November 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi: 10.1016/j.cell.2006.09.026 . PMID   17081983. S2CID   7827573.
  16. ↑ "CCDC82". NetPhos 2.0. Center for Biological Analysis. Archived from the original on 2013-10-16. Retrieved 2013-05-14.
  17. ↑ "CCDC82". Gene Cards. Weizmann Institute of Science. Retrieved 2 May 2013.
  18. ↑ Antoshechkin A, Olalde J, Magarici M, Muhammad A, Salom A, Suarez J, Amendola F (August 2007). "Analysis of effects of the herbal preparation Circulat on gene expression levels in cultured human fibroblasts". Phytother Res. 21 (8): 777–89. doi:10.1002/ptr.2174. PMID   17514633. S2CID   29862628.
  19. ↑ "About Circulat". Circulat. Circulat Biotech LLC. Archived from the original on 2013-08-31.
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