CLSPN

CLSPN
Identifiers
Aliases	CLSPN , claspin
External IDs	OMIM: 605434; MGI: 2445153; HomoloGene: 11138; GeneCards: CLSPN; OMA:CLSPN - orthologs
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1p34.3 Start 35,720,218 bp [1] End 35,769,978 bp [1]
Gene location (Mouse) Chr. Chromosome 4 (mouse) [2] Band 4|4 D2.2 Start 126,450,728 bp [2] End 126,487,696 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in testicle gonad ganglionic eminence ventricular zone bone marrow cells stromal cell of endometrium pancreatic ductal cell appendix buccal mucosa cell right testis Top expressed in tail of embryo hand zygote secondary oocyte genital tubercle primary oocyte otic vesicle ventricular zone maxillary prominence epiblast More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding protein binding anaphase-promoting complex binding Cellular component nucleus nucleoplasm Golgi apparatus Biological process DNA replication cell cycle DNA damage checkpoint signaling mitotic DNA replication checkpoint signaling peptidyl-serine phosphorylation activation of protein kinase activity cellular response to DNA damage stimulus DNA repair protein deubiquitination mitotic G2 DNA damage checkpoint signaling Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 63967 269582 Ensembl ENSG00000092853 ENSMUSG00000042489 UniProt Q9HAW4 Q80YR7 RefSeq (mRNA) NM_001190481 NM_022111 NM_001330490 NM_175554 RefSeq (protein) NP_001177410 NP_001317419 NP_071394 NP_780763 NP_001392727 NP_001392728 NP_001392729 NP_001392730 Location (UCSC) Chr 1: 35.72 – 35.77 Mb Chr 4: 126.45 – 126.49 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Claspin is a protein that in humans is encoded by the CLSPN gene. ^{[5] [6] [7]}

Function

Xenopus claspin is an essential upstream regulator of checkpoint kinase 1 and triggers a checkpoint arrest of the cell cycle in the presence of DNA templates in Xenopus egg extracts. The human gene appears to be the homolog Xenopus claspin and its function has not been determined. ^[7]

Interactions

CLSPN has been shown to interact with:

• BRCA1, ^[8]
• CDC45, ^[9]
• CHEK1, ^[6]
• POLE, ^[9]
• RAD17, ^[9] and
• USP7. ^[10]

References

1. GRCh38: Ensembl release 89: ENSG00000092853 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000042489 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Kumagai A, Dunphy WG (Nov 2000). "Claspin, a novel protein required for the activation of Chk1 during a DNA replication checkpoint response in Xenopus egg extracts". Mol Cell. 6 (4): 839–49. doi: 10.1016/S1097-2765(05)00092-4 . PMID   11090622.
6. Chini CC, Chen J (Aug 2003). "Human claspin is required for replication checkpoint control". J Biol Chem. 278 (32): 30057–62. doi: 10.1074/jbc.M301136200 . PMID   12766152.
7. "Entrez Gene: CLSPN claspin homolog (Xenopus laevis)".
8. Lin SY, Li K, Stewart GS, Elledge SJ (2004). "Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation". Proc. Natl. Acad. Sci. U.S.A. 101 (17): 6484–9. Bibcode:2004PNAS..101.6484L. doi: 10.1073/pnas.0401847101 . PMC   404071 . PMID   15096610.
9. Serçin O, Kemp MG (May 2011). "Characterization of functional domains in human Claspin". Cell Cycle. 10 (10): 1599–606. doi:10.4161/cc.10.10.15562. PMC   3127160 . PMID   21478680.
10. Faustrup H, Bekker-Jensen S, Bartek J, Lukas J, Mailand N (2009). "USP7 counteracts SCFbetaTrCP- but not APCCdh1-mediated proteolysis of Claspin". J. Cell Biol. 184 (1): 13–9. doi:10.1083/jcb.200807137. PMC   2615094 . PMID   19124652.

External links

• Human CLSPN genome location and CLSPN gene details page in the UCSC Genome Browser .

Further reading

• Chini CC, Chen J (2005). "Claspin, a regulator of Chk1 in DNA replication stress pathway". DNA Repair (Amst.). 3 (8–9): 1033–7. doi:10.1016/j.dnarep.2004.03.001. PMID   15279790.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Lin SY, Li K, Stewart GS, Elledge SJ (2004). "Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation". Proc. Natl. Acad. Sci. U.S.A. 101 (17): 6484–9. Bibcode:2004PNAS..101.6484L. doi: 10.1073/pnas.0401847101 . PMC   404071 . PMID   15096610.
• Sørensen CS, Syljuåsen RG, Lukas J, Bartek J (2006). "ATR, Claspin and the Rad9-Rad1-Hus1 complex regulate Chk1 and Cdc25A in the absence of DNA damage". Cell Cycle. 3 (7): 941–5. doi: 10.4161/cc.3.7.972 . PMID   15190204.
• Sar F, Lindsey-Boltz LA, Subramanian D, et al. (2004). "Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures". J. Biol. Chem. 279 (38): 39289–95. doi: 10.1074/jbc.M405793200 . PMID   15226314.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Clarke CA, Clarke PR (2005). "DNA-dependent phosphorylation of Chk1 and Claspin in a human cell-free system". Biochem. J. 388 (Pt 2): 705–12. doi:10.1042/BJ20041966. PMC   1138979 . PMID   15707391.
• Clarke CA, Bennett LN, Clarke PR (2005). "Cleavage of claspin by caspase-7 during apoptosis inhibits the Chk1 pathway". J. Biol. Chem. 280 (42): 35337–45. doi: 10.1074/jbc.M506460200 . PMID   16123041.
• Chini CC, Wood J, Chen J (2006). "Chk1 is required to maintain claspin stability". Oncogene. 25 (30): 4165–71. doi:10.1038/sj.onc.1209447. PMID   16501606. S2CID   21444980.
• Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315–21. Bibcode:2006Natur.441..315G. doi: 10.1038/nature04727 . PMID   16710414.
• Bennett LN, Clarke PR (2006). "Regulation of Claspin degradation by the ubiquitin-proteosome pathway during the cell cycle and in response to ATR-dependent checkpoint activation". FEBS Lett. 580 (17): 4176–81. doi:10.1016/j.febslet.2006.06.071. PMID   16828751. S2CID   42664720.
• Liu S, Bekker-Jensen S, Mailand N, et al. (2006). "Claspin operates downstream of TopBP1 to direct ATR signaling towards Chk1 activation". Mol. Cell. Biol. 26 (16): 6056–64. doi:10.1128/MCB.00492-06. PMC   1592810 . PMID   16880517.
• Mailand N, Bekker-Jensen S, Bartek J, Lukas J (2006). "Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress". Mol. Cell. 23 (3): 307–18. doi: 10.1016/j.molcel.2006.06.016 . PMID   16885021.
• Peschiaroli A, Dorrello NV, Guardavaccaro D, et al. (2006). "SCFbetaTrCP-mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response". Mol. Cell. 23 (3): 319–29. doi: 10.1016/j.molcel.2006.06.013 . PMID   16885022.
• Mamely I, van Vugt MA, Smits VA, et al. (2006). "Polo-like kinase-1 controls proteasome-dependent degradation of Claspin during checkpoint recovery". Curr. Biol. 16 (19): 1950–5. Bibcode:2006CBio...16.1950M. doi: 10.1016/j.cub.2006.08.026 . PMID   16934469.
• Rodríguez-Bravo V, Guaita-Esteruelas S, Florensa R, et al. (2007). "Chk1- and claspin-dependent but ATR/ATM- and Rad17-independent DNA replication checkpoint response in HeLa cells". Cancer Res. 66 (17): 8672–9. doi: 10.1158/0008-5472.CAN-05-4443 . PMID   16951182.
• Chini CC, Chen J (2006). "Repeated phosphopeptide motifs in human Claspin are phosphorylated by Chk1 and mediate Claspin function". J. Biol. Chem. 281 (44): 33276–82. doi: 10.1074/jbc.M604373200 . PMID   16963448.
• Sercin O, Kemp MG (2011). "Characterization of functional domains in human Claspin". Cell Cycle. 10 (10): 1599–606. doi:10.4161/cc.10.10.15562. PMC   3127160 . PMID   21478680.