CSTF3

CSTF3
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	2OND, 2OOE
Identifiers
Aliases	CSTF3 , CSTF-77, cleavage stimulation factor subunit 3
External IDs	OMIM: 600367 MGI: 1351825 HomoloGene: 1014 GeneCards: CSTF3
Gene location (Human)
Chr.	Chromosome 11 (human)
End	33,162,371 bp
Gene location (Mouse)
Chr.	Chromosome 2 (mouse)
End	104,495,774 bp
RNA expression pattern
	Top expressed in
	tendon; ; prosencephalon; ; anterior pituitary; ; pituitary gland; ; sural nerve; ; lung; ; stomach; ; kidney;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	GO:0001948 protein binding ; mRNA binding ; RNA binding ;
Cellular component	nucleus ; nucleoplasm ;
Biological process	mRNA splicing, via spliceosome ; termination of RNA polymerase II transcription ; mRNA polyadenylation ; mRNA cleavage ; RNA processing ; mRNA processing ; mRNA 3'-end processing ; RNA 3'-end processing ;
	Sources:Amigo / QuickGO
Orthologs
	1479
	228410
	ENSG00000176102
	ENSMUSG00000027176
	Q12996
	Q99LI7
	NM_001033505 ; NM_001033506 ; NM_001326
	NM_001037326 ; NM_145529 ; NM_177253
	NP_001028677 ; NP_001028678 ; NP_001317
	NP_001032403 ; NP_663504 ; NP_796227
	Wikidata
View/Edit Human	View/Edit Mouse

Last updated March 28, 2022

Cleavage stimulation factor 77 kDa subunit is a protein that in humans is encoded by the CSTF3 gene.^[5]^[6]

The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms.^[6]

Interactions

CSTF3 has been shown to interact with CSTF2.^[7]

Related Research Articles

Cleavage and polyadenylation specificity factor (CPSF) is involved in the cleavage of the 3' signaling region from a newly synthesized pre-messenger RNA (pre-mRNA) molecule in the process of gene transcription. It is the first protein to bind to the signaling region near the cleavage site of the pre-mRNA, to which the poly(A) tail will be added by polynucleotide adenylyltransferase. The upstream signaling region has the canonical nucleotide sequence AAUAAA, which is highly conserved across the vast majority of pre-mRNAs. A second downstream signaling region, located on the portion of the pre-mRNA that is cleaved before polyadenylation, consists of a GU-rich region required for efficient processing.

Eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of transportable complementary RNA replica. Gene transcription occurs in both eukaryotic and prokaryotic cells. Unlike prokaryotic RNA polymerase that initiates the transcription of all different types of RNA, RNA polymerase in eukaryotes comes in three variations, each translating a different type of gene. A eukaryotic cell has a nucleus that separates the processes of transcription and translation. Eukaryotic transcription occurs within the nucleus where DNA is packaged into nucleosomes and higher order chromatin structures. The complexity of the eukaryotic genome necessitates a great variety and complexity of gene expression control.

5'-3' Exoribonuclease 2 (XRN2) also known as Dhm1-like protein is an exoribonuclease enzyme that in humans is encoded by the XRN2 gene.

DNA-directed RNA polymerase II subunit RPB4 is an enzyme that in humans is encoded by the POLR2D gene.

Splicing factor U2AF 35 kDa subunit is a protein that in humans is encoded by the U2AF1 gene.

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene.

Cleavage and polyadenylation specificity factor subunit 5 (CPSF5) is an enzyme that in humans is encoded by the NUDT21 gene. It belongs to the Nudix family of hydrolases.

Cleavage and polyadenylation specificity factor subunit 2 is a protein that in humans is encoded by the CPSF2 gene.

Cleavage and polyadenylation specificity factor subunit 1 is a protein that in humans is encoded by the CPSF1 gene.

DNA polymerase delta subunit 2 is an enzyme that in humans is encoded by the POLD2 gene. It is a component of the DNA polymerase delta complex.

Poly(A) polymerase alpha is an enzyme that in humans is encoded by the PAPOLA gene.

Symplekin is a protein that in humans is encoded by the SYMPK gene.

Cleavage stimulation factor 50 kDa subunit is a protein that in humans is encoded by the CSTF1 gene.

Cleavage stimulation factor 64 kDa subunit, tau variant is a protein that in humans is encoded by the CSTF2T gene.

Cleavage and polyadenylation specificity factor subunit 3 is a protein that in humans is encoded by the CPSF3 gene.

Cleavage and polyadenylation specificity factor subunit 4 is a protein that in humans is encoded by the CPSF4 gene.

Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene.

Integrator complex subunit 11 is a protein that in humans is encoded by the CPSF3L gene.

Transcription initiation factor TFIID subunit 9B is a protein that in humans is encoded by the TAF9B gene.

Cleavage and polyadenylation specificity factor subunit 7 is a protein that in humans is encoded by the CPSF7 gene.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000176102 - Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000027176 - Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Takagaki Y, Manley JL (December 1994). "A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein". Nature. 372 (6505): 471–4. Bibcode:1994Natur.372..471T. doi:10.1038/372471a0. PMID 7984242. S2CID 4329672.
1 2 "Entrez Gene: CSTF3 cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa".
↑ Takagaki, Y; Manley J L (March 2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component". Mol. Cell. Biol. 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. ISSN 0270-7306. PMC 85326 . PMID 10669729.

External links

Human CSTF3 genome location and CSTF3 gene details page in the UCSC Genome Browser .

Takagaki Y, Manley JL (1992). "A human polyadenylation factor is a G protein beta-subunit homologue". J. Biol. Chem. 267 (33): 23471–4. doi: 10.1016/S0021-9258(18)35861-7 . PMID 1358884.
Takagaki Y, MacDonald CC, Shenk T, Manley JL (1992). "The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs". Proc. Natl. Acad. Sci. U.S.A. 89 (4): 1403–7. Bibcode:1992PNAS...89.1403T. doi: 10.1073/pnas.89.4.1403 . PMC 48459 . PMID 1741396.
Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes Dev. 9 (21): 2672–83. doi: 10.1101/gad.9.21.2672 . PMID 7590244.
Takagaki Y, Manley JL (2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component". Mol. Cell. Biol. 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. PMC 85326 . PMID 10669729.
Emili A, Schieltz DM, Yates JR, Hartwell LH (2001). "Dynamic interaction of DNA damage checkpoint protein Rad53 with chromatin assembly factor Asf1". Mol. Cell. 7 (1): 13–20. doi: 10.1016/S1097-2765(01)00150-2 . PMID 11172707.
Benoit B, Juge F, Iral F, et al. (2002). "Chimeric human CstF-77/Drosophila Suppressor of forked proteins rescue suppressor of forked mutant lethality and mRNA 3' end processing in Drosophila". Proc. Natl. Acad. Sci. U.S.A. 99 (16): 10593–8. Bibcode:2002PNAS...9910593B. doi: 10.1073/pnas.162191899 . PMC 124984 . PMID 12149458.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC 139241 . PMID 12477932.
Kaufmann I, Martin G, Friedlein A, et al. (2005). "Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase". EMBO J. 23 (3): 616–26. doi:10.1038/sj.emboj.7600070. PMC 1271804 . PMID 14749727.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928 . PMID 15489334.
Kim J, Bhinge AA, Morgan XC, Iyer VR (2005). "Mapping DNA-protein interactions in large genomes by sequence tag analysis of genomic enrichment". Nat. Methods. 2 (1): 47–53. doi:10.1038/nmeth726. PMID 15782160. S2CID 6135437.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Taylor TD, Noguchi H, Totoki Y, et al. (2006). "Human chromosome 11 DNA sequence and analysis including novel gene identification". Nature. 440 (7083): 497–500. Bibcode:2006Natur.440..497T. doi: 10.1038/nature04632 . PMID 16554811.
Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi: 10.1016/j.cell.2006.09.026 . PMID 17081983. S2CID 7827573.
Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948 . PMID 17353931.

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[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000176102 - Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000027176 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid7984242-5] Takagaki Y, Manley JL (December 1994). "A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein". Nature. 372 (6505): 471–4. Bibcode:1994Natur.372..471T. doi:10.1038/372471a0. PMID 7984242. S2CID 4329672.

[entrez-6] 1 2 "Entrez Gene: CSTF3 cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa".

[pmid10669729-7] Takagaki, Y; Manley J L (March 2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component". Mol. Cell. Biol. 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. ISSN 0270-7306. PMC 85326 . PMID 10669729.

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CSTF3

Contents

Interactions

Related Research Articles

References

External links

Further reading