Chilomastix | |
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Electron micrograph of Chilomastix cuspidata . The "9+2" axoneme and the enclosing membrane can be seen, the flagellum has two vanes. | |
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Genus: | Chilomastix Aléxéieff, 1910 |
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Chilomastix is a genus of pyriform excavates within the family Retortamonadidae [1] All species within this genus are flagellated, structured with three flagella pointing anteriorly and a fourth contained within the feeding groove. [1] Chilomastix also lacks Golgi apparatus and mitochondria but does possess a single nucleus. [1] The genus parasitizes a wide range of vertebrate hosts, but is known to be typically non-pathogenic, and is therefore classified as harmless. [2] [3] The life cycle of Chilomastix lacks an intermediate host or vector. [4] Chilomastix has a resistant cyst stage responsible for transmission and a trophozoite stage, which is recognized as the feeding stage. Chilomastix mesnili is one of the more studied species in this genus due to the fact it is a human parasite. Therefore, much of the information on this genus is based on what is known about this one species.
The nomenclature of this genus has been under much dispute throughout history, switching between many different taxonomic names. The first known report of this genus was in 1854 when patients in Paris showed infection by a parasitic flagellate. [5] The human parasite was classified under the name of Cercomonas hominis var. 1 by Davaine (1854). [6] In 1910, it was reclassified as its own species and named Macrostoma mesnili by Charles Morley Wenyon, an English protozoologist. [5] It was again renamed to Chilomastix davainei (syn. Chilomastix mesnili) in 1920 by Kofoid. [5] The origin of the name Chilomastix however, was before 1920. The initial proposal of Chilomastix being its own genus was urged by Alexeieffe in 1912 who was the first to thoroughly describe a species belonging to this genus. [5] Although he initially called it Tetramitus caulleryi in 1910, he then referred it is as its own genus, Chilomastix in 1912. [5]
Chilomastix has a worldwide distribution; Chilomastix mesnili is considered a cosmopolitan species, having been found in marine, freshwater and brackish waters. [7] This genus, however, is more prevalent in warmer climates. [3]
The cyst stage of the organism if often found in feces where it can thrive away from a host, until it is ingested. [4] The trophozoite stage resides in the intestine of its host where it feeds on intestinal bacteria. [4]
Species within this genus are known to parasitize a wide variety of mammalian hosts including humans, monkeys, chimpanzees, apes, and pigs. [8] Bird hosts, as seen in ostriches, rheas, chickens and geese, as well as invertebrate hosts such as insects have also been documented. [9] [10]
The life cycle is direct, requiring no intermediate host or vector. [4] Chilomastix exists as a cyst stage that is responsible for transmission and a trophozoite stage which is also known as the feeding stage. Transmission occurs via the fecal-oral route when water contaminated with feces that contain Chilomastix cysts is ingested. [4] The uptake of cysts by the host leads to the excystation of one trophozoite per cyst, which then multiply via binary fission and reside within the intestine of the host. [4] Trophozoites feed on the host's intestinal contents such as bacteria through endocytosis. [11] Once the intestinal contents begin to dry out, the trophozoites then release a cyst wall through the process of encystation. Cysts are resistant and do not require feeding to survive, thus once the food source begins to run out, stimulation of encystation occurs. Both trophozoites and cysts are passed in the feces but only cysts can survive outside of the host and are therefore the stage of infection. [4] When exposed to the external environment, the trophozoites disintegrate while the cysts remain living in bodies of water until they are again taken up by the next host, continuing the cycle of transmission. [4]
Within the intestine of the host, Chilomastix trophozoites feed via endocytosis. [12] This brings the particles into the cell and stimulates the formation of a food vacuole. Chilomastix often feeds on bacteria living inside the gut of the host. [1] The cytosomal flagellum aids in bringing intestinal bacteria closer towards the cell, allowing the membrane to wrap around the food particle and pinch off to form a food vacuole within the cell body. [1] [12] The cyst stage is non-feeding.
Chilomastix species are generally regarded as harmless gut commensals and are non-pathogenic. [3] They are typically asymptomatic as severity of infection is usually no higher than seen in Chilomastix mesnili and C. gallinarum, known to cause watery diarrhea. [3] [13]
Chilomastix species are often transmitted along with other intestinal parasites, many of which are pathogenic and do cause diseases such as Giardia lamblia and Balantidium coli. [14] This commonly causes confusion during diagnosis. Diagnosis is made upon finding one or both cyst and trophozoite forms in feces samples of an infected patient.
Due to the non-pathogenic nature of this taxon, no treatments are required except to relieve the discomfort of the diarrhea in extreme cases. [3] If the infected patient is showing other symptoms of disease, it is most likely due to the presence of another parasitic pathogen and further testing should be done.
Chilomastix cells are not bilaterally symmetrical and lack mitochondria, an axostyle, Golgi apparatus, and an undulating membrane. [15]
Trophozoites of Chilomastix have been described as pyriform, lemon-shaped or pear-shaped in various species with a rounded anterior and an elongated posterior end that comes to a point. [1] [2] Four flagella are present in all species; three flagella extend anteriorly and move freely, while the fourth flagellum is located within the feeding groove that acts as the cell mouth. [1] This fourth, posteriorly orientated flagellum is vaned, due to the presence of two wing-like structures that extend from it. [11] The feeding groove and fourth flagellum are positioned in the anterior region of the body and work together, involved in the function of endocytosis, enabling the movement of food particles towards the feeding groove. [1] The flagella possess the 9+2 structure, common in flagellated eukaryotes. A single distinct nucleus is in the very anterior region of the body near the cytoplasm. [2]
Cysts are lemon or pear shaped, typically rounder and smaller than the trophozoite. [1] [4] One end of the cyst cell is rounded while the other end has a slight, blunt protuberance. [1] The cyst wall is of even thickness except in the region of the protuberance where it is even thicker. [1] Organelles found in the trophozoite, including the nucleus and cytostome, remain in the cyst stage and are usually viewable when stained under the microscope. [15] While the vaned flagellum is present in the cyst stage, the three free anterior flagella are not, thus making the cyst non-motile. [4]
Giardia duodenalis, also known as Giardia intestinalis and Giardia lamblia, is a flagellated parasitic protozoan microorganism of the genus Giardia that colonizes the small intestine, causing a diarrheal condition known as giardiasis. The parasite attaches to the intestinal epithelium by an adhesive disc or sucker, and reproduces via binary fission. Giardiasis does not spread to other parts of the gastrointestinal tract, but remains confined to the lumen of the small intestine. The microorganism has an outer membrane that makes it possible to survive even when outside of its host, and which can render it tolerant to certain disinfectants. Giardia trophozoites are anaerobic, and absorb their nutrients from the intestinal lumen. If the organism is stained, its characteristic pattern resembles the familiar "smiley face" symbol.
The opalines are a small group of peculiar heterokonts, currently assigned to the family Opalinidae, in the order Slopalinida. Their name is derived from the opalescent appearance of these microscopic organisms when illuminated with full sunlight. Most opalines live in the large intestine and cloaca of anurans, though they are sometimes found in fish, reptiles, molluscs and insects; whether they are parasitic is not certain. The unusual features of the opalines, first observed by Antonie van Leeuwenhoek in 1683, has led to much debate regarding their phylogenetic position among the protists.
Giardiasis is a parasitic disease caused by Giardia duodenalis. Infected individuals who experience symptoms may have diarrhoea, abdominal pain, and weight loss. Less common symptoms include vomiting and blood in the stool. Symptoms usually begin one to three weeks after exposure and, without treatment, may last two to six weeks or longer.
Naegleria is a genus consisting of 47 described species of protozoa often found in warm aquatic environments as well as soil habitats worldwide. It has three life cycle forms: the amoeboid stage, the cyst stage, and the flagellated stage, and has been routinely studied for its ease in change from amoeboid to flagellated stages. The Naegleria genera became famous when Naegleria fowleri, the causative agent of the usually fatal human and animal disease primary amoebic meningoencephalitis (PAM), was discovered in 1965. Most species in the genus, however, are incapable of causing disease.
Balantidium coli is a parasitic species of ciliate alveolates that causes the disease balantidiasis. It is the only member of the ciliate phylum known to be pathogenic to humans.
A trophozoite is the activated, feeding stage in the life cycle of certain protozoa such as malaria-causing Plasmodium falciparum and those of the Giardia group. The complementary form of the trophozoite state is the thick-walled cyst form. They are often different from the cyst stage, which is a protective, dormant form of the protozoa. Trophozoites are often found in the host's body fluids and tissues and in many cases, they are the form of the protozoan that causes disease in the host. In the protozoan, Entamoeba histolytica it invades the intestinal mucosa of its host, causing dysentery, which aid in the trophozoites traveling to the liver and leading to the production of hepatic abscesses.
Retortamonas is a genus of flagellated excavates. It is one of only two genera belonging to the family Retortamonadidae along with the genus Chilomastix. The genus parasitizes a large range of hosts including humans. Species within this genus are considered harmless commensals which reside in the intestine of their host. The wide host diversity is a useful factor given that species are distinguished based on their host rather than morphology. This is because all species share similar morphology, which would present challenges when trying to make classifications based on structural anatomy. Although Retortamonas currently includes over 25 known species, it is possible that some defined species are synonymous, given that such overlapping species have been discovered in the past. Further efforts into learning about this genus must be done such as cross-transmission testing as well as biochemical and genetic studies. One of the most well-known species within this genus is Retortamonas intestinalis, a human parasite that lives in the large intestine of humans.
Balantidiasis is a protozoan infection caused by infection with Balantidium coli.
A microbial cyst is a resting or dormant stage of a microorganism, that can be thought of as a state of suspended animation in which the metabolic processes of the cell are slowed and the cell ceases all activities like feeding and locomotion. Many groups of single-celled, microscopic organisms, or microbes, possess the ability to enter this dormant state.
Chilomastix mesnili is a non-pathogenic member of primate gastrointestinal microflora, commonly associated with but not causing parasitic infections. It is found in about 3.5% of the population in the United States. In addition to humans, Chilomastix is found in chimpanzees, orangutans, monkeys, and pigs. It lives in the cecum and colon. C. mesnili has a similar life style to Giardia lamblia.
Protozoan infections are parasitic diseases caused by organisms formerly classified in the kingdom Protozoa. These organisms are now classified in the supergroups Excavata, Amoebozoa, Harosa, and Archaeplastida. They are usually contracted by either an insect vector or by contact with an infected substance or surface.
Amoebiasis, or amoebic dysentery, is an infection of the intestines caused by a parasitic amoeba Entamoeba histolytica. Amoebiasis can be present with no, mild, or severe symptoms. Symptoms may include lethargy, loss of weight, colonic ulcerations, abdominal pain, diarrhea, or bloody diarrhea. Complications can include inflammation and ulceration of the colon with tissue death or perforation, which may result in peritonitis. Anemia may develop due to prolonged gastric bleeding.
Colpodella is a genus of alveolates comprising 5 species, and two further possible species: They share all the synapomorphies of apicomplexans, but are free-living, rather than parasitic. Many members of this genus were previously assigned to a different genus - Spiromonas.
Proteromonas is a genus of single-celled biflagellated microbial eukaryotes belonging to the Superphylum Stramenopiles which are characterized by the presence of tripartite, hair-like structures on the anteriorly-directed larger of the two flagella. Proteromonas on the other hand are notable by having tripartite hairs called somatonemes not on the flagella but on the posterior of the cell. Proteromonas are closely related to Karotomorpha and Blastocystis, which belong to the Opalines group.
Willaertia /ˈwɪləɹʃə/ is a genus of non-pathogenic, free-living, thermophilic amoebae in the family Vahlkampfiidae.
Katabia is a genus of soil-dwelling heterotrophic flagellate cercozoans containing the single species Katabia gromovi, and the only member of family Katabiidae.
Dientamoeba fragilis is a species of single-celled excavates found in the gastrointestinal tract of some humans, pigs and gorillas. It causes gastrointestinal upset in some people, but not in others. It is an important cause of traveller's diarrhoea, chronic diarrhoea, fatigue and, in children, failure to thrive. Despite this, its role as a "commensal, pathobiont, or pathogen" is still debated. D. fragilis is one of the smaller parasites that are able to live in the human intestine. Dientamoeba fragilis cells are able to survive and move in fresh feces but are sensitive to aerobic environments. They dissociate when in contact or placed in saline, tap water or distilled water.
Entamoeba invadens is an amoebozoa parasite of reptiles, within the genus Entamoeba. It is closely related to the human parasite Entamoeba histolytica, causing similar invasive disease in reptiles, in addition to a similar morphology and lifecycle.
Cochlosoma is a genus of flagellated protozoa in the order Trichomonadida created by A. Kotlán (1923). Some of their typical features include a prominent adhesive disc, axostyle, costa, and six flagella – one of which is attached to an undulating membrane that runs laterally along the body.
Monocercomonas is a Parabasalian genus belonging to the order Trichomonadida. It presents four flagella, three forward-facing and one trailing, without the presence of a costa or any kind of undulating membrane. Monocercomonas is found in animal guts. and is susceptible to cause Monocercomoniasis in reptiles