Cowpea mosaic virus

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Cowpea mosaic virus
CowpeaMosaicVirus3D.png
Structure of the Cowpea Mosaic Virus based on PDB 2BFU
Virus classification Red Pencil Icon.png
(unranked): Virus
Realm: Riboviria
Kingdom: Orthornavirae
Phylum: Pisuviricota
Class: Pisoniviricetes
Order: Picornavirales
Family: Secoviridae
Genus: Comovirus
Species:
Cowpea mosaic virus
Synonyms

Cowpea mosaic virus, SB isolate

Cowpea mosaic virus (CPMV) is a non-enveloped plant virus of the comovirus group. Infection of a susceptible cowpea leaf causes a "mosaic" pattern in the leaf, and results in high virus yields (1-2 g/kg). Its genome consists of 2 molecules of positive-sense RNA (RNA-1 and RNA-2) which are separately encapsidated. Both RNA1 and RNA2 have a VPg (virus genome-linked protein) at the 5'end, and polyadenylation at the 3' end. Genomic RNA1 and RNA2 are expressed by a polyprotein processing strategy. RNA1 encodes helicase, VPg, protease and RdRp. RNA2 encodes movement protein and coat protein. The virus particles are 28 nm in diameter and contain 60 copies each of a Large (L) and Small (S) coat protein. The structure is well characterised to atomic resolution, and the viral particles are thermostable. The identification of the virus is attributed to Lister and Thresh in 1955, [1] but it is now known as a variant of the Sunn-hemp mosaic virus. [2]

CPMV displays a number of features that can be exploited for nanoscale biomaterial fabrication. Its genetic, biological and physical properties are well characterised, and it can be isolated readily from plants. There are many stable mutants already prepared that allow specific modification of the capsid surface. It is possible to attach a number of different chemicals to the virus surface [3] [4] and to construct multilayer arrays of such nanoparticles on solid surfaces. This gives the natural or genetically engineered nanoparticles a range of properties which could be useful in nanotechnological applications such as biosensors, catalysis and nanoelectronic devices.

One example use of CPMV particles is to amplify signals in microarray based sensors. In this application, the virus particles separate the fluorescent dyes used for signaling in order to prevent the formation of non-fluorescent dimers that act as quenchers. [5] Another example is the use of CPMV as a nanoscale breadboard for molecular electronics. [6]

CPMV particles have also shown potential for in-situ vaccination in cancer immunotherapy [7]

Related Research Articles

<i>Tobacco mosaic virus</i> Infects tomato family, beans, flowers...

Tobacco mosaic virus (TMV) is a positive-sense single-stranded RNA virus species in the genus Tobamovirus that infects a wide range of plants, especially tobacco and other members of the family Solanaceae. The infection causes characteristic patterns, such as "mosaic"-like mottling and discoloration on the leaves. TMV was the first virus to be discovered. Although it was known from the late 19th century that a non-bacterial infectious disease was damaging tobacco crops, it was not until 1930 that the infectious agent was determined to be a virus. It is the first pathogen identified as a virus. Virus was crystallised by W.M.Stanley.

Picornavirus Family of viruses

Picornaviruses are a group of related nonenveloped RNA viruses which infect vertebrates including fish, mammals, and birds. They are viruses that represent a large family of small, positive-sense, single-stranded RNA viruses with a 30 nm icosahedral capsid. The viruses in this family can cause a range of diseases including the common cold, poliomyelitis, meningitis, hepatitis, and paralysis.

Cauliflower mosaic virus (CaMV) is a member of the genus Caulimovirus, one of the six genera in the family Caulimoviridae, which are pararetroviruses that infect plants. Pararetroviruses replicate through reverse transcription just like retroviruses, but the viral particles contain DNA instead of RNA.

Plant virus Virus that affects plants

Plant viruses are viruses that affect plants. Like all other viruses, plant viruses are obligate intracellular parasites that do not have the molecular machinery to replicate without a host. Plant viruses can be pathogenic to higher plants.

<i>Tobamovirus</i> Genus of viruses

Tobamovirus is a genus of positive-strand RNA viruses in the family Virgaviridae. Many plants, including tobacco, potato, tomato, and squash, serve as natural hosts. Diseases associated with this genus include: necrotic lesions on leaves. The name Tobamovirus comes from the host and symptoms of the first virus discovered.

VPg is a protein that is covalently attached to the 5′ end of positive strand viral RNA and acts as a primer during RNA synthesis in a variety of virus families including Picornaviridae, Potyviridae and Caliciviridae. There are some studies showing that a possible VPg protein is also present in astroviridae, however, experimental evidence for this has not yet been provided and requires further study. The primer activity of VPg occurs when the protein becomes uridylated, providing a free hydroxyl that can be extended by the virally encoded RNA-dependent RNA polymerase. For some virus families, VPg also has a role in translation initiation by acting like a 5' mRNA cap.

Movement protein

Successful infection of a plant by a plant virus depends on its ability to move from the cell initially infected to neighbouring cells in order to spread infection. Unlike animal cells, plant cells have robust cell walls, which viruses cannot easily penetrate. A movement protein is a non-structural protein which is encoded by some plant viruses to allow their movement from one infected cell to neighbouring cells. Many, if not all, plant viruses encode a movement protein, and some express more than one. The movement protein of tobacco mosaic virus (TMV) has been most extensively studied. Plant viruses can also be transported over longer distances through the host plant in the vascular system via the phloem.

<i>Potyvirus</i> Genus of positive-strand RNA viruses in the family Potyviridae

Potyvirus is a genus of positive-strand RNA viruses in the family Potyviridae. Plants serve as natural hosts. The genus is named after member virus potato virus Y. Potyviruses account for about thirty percent of the currently known plant viruses. Like begomoviruses, members of this genus may cause significant losses in agricultural, pastoral, horticultural, and ornamental crops. More than 200 species of aphids spread potyviruses, and most are from the subfamily Aphidinae. The genus contains 190 species.

The murine leukemia viruses are retroviruses named for their ability to cause cancer in murine (mouse) hosts. Some MLVs may infect other vertebrates. MLVs include both exogenous and endogenous viruses. Replicating MLVs have a positive sense, single-stranded RNA (ssRNA) genome that replicates through a DNA intermediate via the process of reverse transcription.

<i>Cowpea chlorotic mottle virus</i> Species of virus

Cowpea chlorotic mottle virus, known by the abbreviation CCMV, is a virus that specifically infects the cowpea plant, or black-eyed pea. The leaves of infected plants develop yellow spots, hence the name "chlorotic". Similar to its "brother" virus, Cowpea mosaic virus (CPMV), CCMV is produced in high yield in plants. In the natural host, viral particles can be produced at 1–2 mg per gram of infected leaf tissue. Belonging to the bromovirus genus, cowpea chlorotic mottle virus (CCMV) is a small spherical plant virus. Other members of this genus include the brome mosaic virus (BMV) and the broad bean mottle virus (BBMV).

<i>Alfalfa mosaic virus</i> Species of virus

Alfalfa mosaic virus (AMV), also known as Lucerne mosaic virus or Potato calico virus, is a worldwide distributed phytopathogen that can lead to necrosis and yellow mosaics on a large variety of plant species, including commercially important crops. It is the only Alfamovirus of the family Bromoviridae. In 1931 Weimer J.L. was the first to report AMV in alfalfa. Transmission of the virus occurs mainly by some aphids, by seeds or by pollen to the seed.

Clover yellow mosaic virus (ClYMV) is a plant pathogenic virus in the genus Potexvirus and the virus family Alphaflexiviridae. Its flexous rod-shaped particles measure about 539 nm in length.

<i>Cucumber mosaic virus</i> Species of virus

Cucumber mosaic virus (CMV) is a plant pathogenic virus in the family Bromoviridae. This virus has a worldwide distribution and a very wide host range. In fact it has the reputation of having the widest host range of any known plant virus. It can be transmitted from plant to plant both mechanically by sap and by aphids in a stylet-borne fashion. It can also be transmitted in seeds and by the parasitic weeds, Cuscuta sp. (dodder).

Sunn-hemp mosaic virus (SHMV) is a pathogenic plant virus. It is known by many names, including bean strain of tobacco mosaic virus and Sunn-hemp rosette virus. SHMV is an intracellular parasite that infects plants. It can be seen only through an electron microscope. It is a positive-sense single-stranded RNA virus that causes physical characteristics of spotting and/or discoloration. The Sunn-hemp mosaic virus is listed as a species within the genus Tobamovirus and the virus family Virgaviridae by the International Committee on the Taxonomy of Viruses, who renamed it from "Sunnhemp" to "Sunn-hemp" in 1991.

White clover mosaic virus (WClMV) is a plant pathogenic virus in the genus Potexvirus and the family Alphaflexiviridae. WClMV is a filamentous, flexuous rod, 480 nm in length and 13 nm wide.

Soil-borne wheat mosaic virus is a rod-shaped plant pathogen that can cause severe stunting and mosaic in susceptible wheat, barley and rye cultivars. The disease has often been misdiagnosed as a nutritional problem, but this has actually allowed in part for the fortuitous visual selection by breeding programs of resistant genotypes. Soil-borne wheat mosaic virus is part of the genus Furovirus. Members of this genus are characterized by rigid rod-shaped particles and positive sense RNA genomes consisting of two molecules that are packaged into separate particles that code for either replication, mobility, structure or defense against the host. The virus is spread by a fungal-like protist, Polymyxa graminis, whose asexual secondary and sexual primary cycles help the virus spread. The disease produces secondary symptoms from the root cell infection. The disease is a serious contributor to loss in crop yield.

Virus Small non-cellular infectious agent that only replicates in cells

A virus is a submicroscopic infectious agent that replicates only inside the living cells of an organism. Viruses infect all life forms, from animals and plants to microorganisms, including bacteria and archaea. Since Dmitri Ivanovsky's 1892 article describing a non-bacterial pathogen infecting tobacco plants and the discovery of the tobacco mosaic virus by Martinus Beijerinck in 1898, more than 9,000 virus species have been described in detail of the millions of types of viruses in the environment. Viruses are found in almost every ecosystem on Earth and are the most numerous type of biological entity. The study of viruses is known as virology, a subspeciality of microbiology.

Nanodiamond Extremely small diamonds used for their thermal, mechanical and optoelectronic properties

Nanodiamonds, or diamond nanoparticles, are diamonds with a size below 1 micrometre. They can be produced by impact events such as an explosion or meteoritic impacts. Because of their inexpensive, large-scale synthesis, potential for surface functionalization, and high biocompatibility, nanodiamonds are widely investigated as a potential material in biological and electronic applications and quantum engineering.

CytoViva, Inc. is a scientific imaging and instrumentation company that develops and markets optical microscopy and hyperspectral imaging technology for nanomaterials, pathogen and general biology applications.

Virus nanotechnology is the use of viruses as a source of nanoparticles for biomedical purposes. Viruses are made up of a genome and a capsid; and some viruses are enveloped. Most virus capsids measure between 20-500 nm in diameter. Because of their nanometer size dimensions, viruses have been considered as naturally occurring nanoparticles. Virus nanoparticles have been subject to the nanoscience and nanoengineering disciplines. Viruses can be regarded as prefabricated nanoparticles. Many different viruses have been studied for various applications in nanotechnology: for example, mammalian viruses are being developed as vectors for gene delivery, and bacteriophages and plant viruses have been used in drug delivery and imaging applications as well as in vaccines and immunotherapy intervention.

References

  1. Lister, R. M.; Thresh, J. M. (1955). "A mosaic disease of leguminous plants caused by a strain of tobacco mosaic virus". Nature . 175 (4467): 1047–1048. Bibcode:1955Natur.175.1047L. doi:10.1038/1751047a0. PMID   14394105. S2CID   4197728.
  2. Varma, Anupam (1986). "Sunn-Hemp Mosaic Virus". In Van Regenmortel, M. H. V.; Fraenkel-Conrat, Heinz (eds.). The Plant Viruses: The Rod-Shaped Plant Viruses. 2. Plenum Press. pp. 249–266. doi:10.1007/978-1-4684-7026-0_13. ISBN   9781468470260.
  3. Q. Wang, T. Lin, L. Tang, J.E. Johnson, and M.G. Finn.Angew. Chem. Int. Ed., 41(3), 459 (2002)
  4. Q. Wang, T.R. Chan, R. Hilgraf, V.V. Fokin, K.B. Sharpless, and M.G. Finn. J. Am. Chem. Soc., 125, 3192 (2003).
  5. Fluorescent signal amplification of carbocyanine dyes using engineered viral nanoparticles. Carissa M. Soto, Amy Szuchmacher Blum, Nikolai Lebedev, Gary J. Vora, Carolyn E. Meador, Angela P. Won, Anju Chatterji, John E. Johnson, and Banahalli R. Ratna, Journal of the American Chemical Society, 128, 5184 (2006).
  6. An Engineered Virus as a Scaffold for Three-Dimensional Self-Assembly on the Nanoscale. Amy Szuchmacher Blum, Carissa M. Soto, Charmaine D. Wilson, Tina L. Brower, Steven K. Pollack, Terence L. Schull, Anju Chatterji, Tianwei Lin, John E. Johnson, Christian Amsinck, Paul Franzon, Ranganathan Shashidhar and Banahalli Ratna, Small, 7, 702 (2005).
  7. "Plant Virus Used as Cancer Immunotherapy Agent". 2015-12-23.