DLGAP1

DLGAP1
Identifiers
Aliases	DLGAP1 , DAP-1, DAP-1-ALPHA, DAP-1-BETA, DAP1, DLGAP1A, DLGAP1B, GKAP, SAPAP1, hGKAP, discs large homolog associated protein 1, DLG associated protein 1
External IDs	OMIM: 605445; MGI: 1346065; HomoloGene: 31258; GeneCards: DLGAP1; OMA:DLGAP1 - orthologs
Gene location (Human) Chr. Chromosome 18 (human) [1] Band 18p11.31 Start 3,496,032 bp [1] End 4,455,307 bp [1]
Gene location (Mouse) Chr. Chromosome 17 (mouse) [2] Band 17 E1.3|17 40.85 cM Start 70,276,068 bp [2] End 71,128,408 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Brodmann area 23 endothelial cell primary visual cortex prefrontal cortex pons dorsolateral prefrontal cortex Brodmann area 9 parietal lobe superior frontal gyrus right frontal lobe Top expressed in piriform cortex subiculum primary motor cortex cingulate gyrus subdivision of hippocampus Region I of hippocampus proper lateral septal nucleus cerebellar vermis lobe of cerebellum anterior amygdaloid area More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding protein domain specific binding protein-containing complex binding Cellular component cell junction postsynaptic membrane postsynaptic density membrane plasma membrane synapse cellular component Biological process signaling chemical synaptic transmission Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9229 224997 Ensembl ENSG00000170579 ENSMUSG00000003279 UniProt O14490 Q9D415 RefSeq (mRNA) NM_001003809 NM_001242761 NM_001242762 NM_001242763 NM_001242764 NM_001242765 NM_001242766 NM_001308390 NM_004746 NM_001128180 NM_001128181 NM_027712 NM_177639 NM_001347411 NM_001347412 NM_001347413 NM_001347414 NM_001360665 RefSeq (protein) NP_001003809 NP_001229690 NP_001229691 NP_001229692 NP_001229693 NP_001229694 NP_001229695 NP_001295319 NP_004737 NP_001121652 NP_001121653 NP_001334340 NP_001334341 NP_001334342 NP_001334343 NP_081988 NP_808307 NP_001347594 Location (UCSC) Chr 18: 3.5 – 4.46 Mb Chr 17: 70.28 – 71.13 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Disks large-associated protein 1 (DAP-1), also known as guanylate kinase-associated protein (GKAP), is a protein that in humans is encoded by the DLGAP1 gene. DAP-1 is known to be highly enriched in synaptosomal preparations of the brain, and present in the post-synaptic density. ^[5]

Function

This gene encodes the protein called guanylate kinase-associated protein (GKAP). GKAP binds to the SHANK2 and PSD-95 proteins, facilitating the assembly of the post-synaptic density of neurons. ^[6] Dlgap1 has five 14-amino-acid repeats and three Pro-rich portions.

Interactions

DLGAP1 has been shown to interact with:

• DLG1 ^{[7] [8] [9] [10]}
• DLG4 ^{[7] [8] [9] [10] [11] [12]}
• DYNLL1 ^[11]
• DYNLL2 ^[11]
• SHANK2 ^{[11] [12]}

The interaction with PSD95 and S-SCAM is mediated by the GUK domain ^[13] and it has been hypothesized that this might mean it can also interact with other GUK containing proteins.

References

1. GRCh38: Ensembl release 89: ENSG00000170579 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000003279 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: DLGAP1 discs, large (Drosophila) homolog-associated protein 1".
6. Hines RM, El-Husseini A (2006). "Mechanisms that regulate neuronal protein clustering at the synapse". In El-Husseini A, Dityatev A (eds.). Molecular mechanisms of synaptogenesis. Berlin: Springer. pp. 72–75. ISBN   978-0-387-32560-6.
7. Takeuchi M, Hata Y, Hirao K, Toyoda A, Irie M, Takai Y (May 1997). "SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density". J. Biol. Chem. 272 (18): 11943–51. doi: 10.1074/jbc.272.18.11943 . PMID   9115257.
8. Satoh K, Yanai H, Senda T, Kohu K, Nakamura T, Okumura N, Matsumine A, Kobayashi S, Toyoshima K, Akiyama T (June 1997). "DAP-1, a novel protein that interacts with the guanylate kinase-like domains of hDLG and PSD-95". Genes Cells. 2 (6): 415–24. doi: 10.1046/j.1365-2443.1997.1310329.x . PMID   9286858.
9. Wu H, Reissner C, Kuhlendahl S, Coblentz B, Reuver S, Kindler S, Gundelfinger ED, Garner CC (November 2000). "Intramolecular interactions regulate SAP97 binding to GKAP". EMBO J. 19 (21): 5740–51. doi:10.1093/emboj/19.21.5740. PMC   305801 . PMID   11060025.
10. Kim E, Naisbitt S, Hsueh YP, Rao A, Rothschild A, Craig AM, Sheng M (February 1997). "GKAP, a novel synaptic protein that interacts with the guanylate kinase-like domain of the PSD-95/SAP90 family of channel clustering molecules". J. Cell Biol. 136 (3): 669–78. doi:10.1083/jcb.136.3.669. PMC   2134290 . PMID   9024696.
11. Naisbitt S, Valtschanoff J, Allison DW, Sala C, Kim E, Craig AM, Weinberg RJ, Sheng M (June 2000). "Interaction of the postsynaptic density-95/guanylate kinase domain-associated protein complex with a light chain of myosin-V and dynein". J. Neurosci. 20 (12): 4524–34. doi: 10.1523/JNEUROSCI.20-12-04524.2000 . PMC   6772433 . PMID   10844022.
12. Boeckers TM, Winter C, Smalla KH, Kreutz MR, Bockmann J, Seidenbecher C, Garner CC, Gundelfinger ED (October 1999). "Proline-rich synapse-associated proteins ProSAP1 and ProSAP2 interact with synaptic proteins of the SAPAP/GKAP family". Biochem. Biophys. Res. Commun. 264 (1): 247–52. Bibcode:1999BBRC..264..247B. doi:10.1006/bbrc.1999.1489. PMID   10527873.
13. Hirao K, Hata Y, Ide N, Takeuchi M, Irie M, Yao I, et al. (1998). "A novel multiple PDZ domain-containing molecule interacting with N-methyl-D-aspartate receptors and neuronal cell adhesion proteins". J Biol Chem. 273 (33): 21105–10. doi: 10.1074/jbc.273.33.21105 . PMID   9694864.

Further reading

• Kim E, Naisbitt S, Hsueh YP, et al. (1997). "GKAP, a novel synaptic protein that interacts with the guanylate kinase-like domain of the PSD-95/SAP90 family of channel clustering molecules". J. Cell Biol. 136 (3): 669–78. doi:10.1083/jcb.136.3.669. PMC   2134290 . PMID   9024696.
• Takeuchi M, Hata Y, Hirao K, et al. (1997). "SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density". J. Biol. Chem. 272 (18): 11943–51. doi: 10.1074/jbc.272.18.11943 . PMID   9115257.
• Naisbitt S, Kim E, Weinberg RJ, et al. (1997). "Characterization of guanylate kinase-associated protein, a postsynaptic density protein at excitatory synapses that interacts directly with postsynaptic density-95/synapse-associated protein 90". J. Neurosci. 17 (15): 5687–96. doi: 10.1523/JNEUROSCI.17-15-05687.1997 . PMC   6573205 . PMID   9221768.
• Satoh K, Yanai H, Senda T, et al. (1997). "DAP-1, a novel protein that interacts with the guanylate kinase-like domains of hDLG and PSD-95". Genes Cells. 2 (6): 415–24. doi: 10.1046/j.1365-2443.1997.1310329.x . PMID   9286858.
• Hirao K, Hata Y, Ide N, et al. (1998). "A novel multiple PDZ domain-containing molecule interacting with N-methyl-D-aspartate receptors and neuronal cell adhesion proteins". J. Biol. Chem. 273 (33): 21105–10. doi: 10.1074/jbc.273.33.21105 . PMID   9694864.
• Deguchi M, Hata Y, Takeuchi M, et al. (1998). "BEGAIN (brain-enriched guanylate kinase-associated protein), a novel neuronal PSD-95/SAP90-binding protein". J. Biol. Chem. 273 (41): 26269–72. doi: 10.1074/jbc.273.41.26269 . PMID   9756850.
• Kawabe H, Hata Y, Takeuchi M, et al. (1999). "nArgBP2, a novel neural member of ponsin/ArgBP2/vinexin family that interacts with synapse-associated protein 90/postsynaptic density-95-associated protein (SAPAP)". J. Biol. Chem. 274 (43): 30914–8. doi: 10.1074/jbc.274.43.30914 . PMID   10521485.
• Boeckers TM, Winter C, Smalla KH, et al. (1999). "Proline-rich synapse-associated proteins ProSAP1 and ProSAP2 interact with synaptic proteins of the SAPAP/GKAP family". Biochem. Biophys. Res. Commun. 264 (1): 247–52. Bibcode:1999BBRC..264..247B. doi:10.1006/bbrc.1999.1489. PMID   10527873.
• Naisbitt S, Valtschanoff J, Allison DW, et al. (2000). "Interaction of the postsynaptic density-95/guanylate kinase domain-associated protein complex with a light chain of myosin-V and dynein". J. Neurosci. 20 (12): 4524–34. doi: 10.1523/JNEUROSCI.20-12-04524.2000 . PMC   6772433 . PMID   10844022.
• Wu H, Reissner C, Kuhlendahl S, et al. (2000). "Intramolecular interactions regulate SAP97 binding to GKAP". EMBO J. 19 (21): 5740–51. doi:10.1093/emboj/19.21.5740. PMC   305801 . PMID   11060025.
• Haraguchi K, Satoh K, Yanai H, et al. (2001). "The hDLG-associated protein DAP interacts with dynein light chain and neuronal nitric oxide synthase". Genes Cells. 5 (11): 905–911. doi: 10.1046/j.1365-2443.2000.00374.x . PMID   11122378.
• Lo KW, Naisbitt S, Fan JS, et al. (2001). "The 8-kDa dynein light chain binds to its targets via a conserved (K/R)XTQT motif". J. Biol. Chem. 276 (17): 14059–66. doi: 10.1074/jbc.M010320200 . PMID   11148209.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Im YJ, Lee JH, Park SH, et al. (2004). "Crystal structure of the Shank PDZ-ligand complex reveals a class I PDZ interaction and a novel PDZ-PDZ dimerization". J. Biol. Chem. 278 (48): 48099–104. doi: 10.1074/jbc.M306919200 . PMID   12954649.
• Ballif BA, Villén J, Beausoleil SA, et al. (2005). "Phosphoproteomic analysis of the developing mouse brain". Mol. Cell. Proteomics. 3 (11): 1093–101. doi: 10.1074/mcp.M400085-MCP200 . PMID   15345747.
• Suzuki T, Li W, Zhang JP, et al. (2005). "A novel scaffold protein, TANC, possibly a rat homolog of Drosophila rolling pebbles (rols), forms a multiprotein complex with various postsynaptic density proteins". Eur. J. Neurosci. 21 (2): 339–50. doi:10.1111/j.1460-9568.2005.03856.x. PMID   15673434. S2CID   28773407.
• Sabio G; Arthur JS; Kuma Y; et al. (2005). "p38gamma regulates the localisation of SAP97 in the cytoskeleton by modulating its interaction with GKAP". EMBO J. 24 (6): 1134–45. doi:10.1038/sj.emboj.7600578. PMC   556394 . PMID   15729360.