EIF2C2

AGO2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3LUC, 3LUD, 3LUG, 3LUH, 3LUJ, 3LUK, 3QX8, 3QX9, 4F3T, 4OLA, 4OLB, 4W5N, 4W5O, 4W5Q, 4W5R, 4W5T, 4Z4C, 4Z4D, 4Z4E, 4Z4F, 4Z4G, 4Z4H, 4Z4I, 5JS1, 5JS2
Identifiers
Aliases	AGO2 , EIF2C2, Q10, argonaute 2, RISC catalytic component, PPD, CASC7, LINC00980, argonaute RISC catalytic component 2, LESKRES
External IDs	OMIM: 606229; MGI: 2446632; HomoloGene: 81825; GeneCards: AGO2; OMA:AGO2 - orthologs
Gene location (Human) Chr. Chromosome 8 (human) [1] Band 8q24.3 Start 140,520,156 bp [1] End 140,635,633 bp [1]
Gene location (Mouse) Chr. Chromosome 15 (mouse) [2] Band 15|15 D3 Start 72,967,693 bp [2] End 73,056,784 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in epithelium of colon cartilage tissue tail of epididymis superficial temporal artery trabecular bone corpus epididymis mucosa of paranasal sinus lower lobe of lung tendon Achilles tendon Top expressed in ascending aorta hand aortic valve human fetus supraoptic nucleus ventromedial nucleus anterior amygdaloid area suprachiasmatic nucleus foot paraventricular nucleus of hypothalamus More reference expression data BioGPS n/a
Gene ontology Molecular function protein binding protein C-terminus binding metal ion binding nuclease activity endoribonuclease activity, cleaving miRNA-paired mRNA hydrolase activity translation initiation factor activity endonuclease activity endoribonuclease activity, cleaving siRNA-paired mRNA single-stranded RNA binding double-stranded RNA binding RNA polymerase II complex binding nucleic acid binding mRNA binding miRNA binding endoribonuclease activity RNA 7-methylguanosine cap binding siRNA binding mRNA cap binding RNA binding endoribonuclease activity, producing 5'-phosphomonoesters Cellular component cytoplasm polysome membrane P-body dendrite RISC-loading complex RISC complex cytosol nucleus nucleoplasm mRNA cap binding complex cell junction extracellular exosome ribonucleoprotein complex Biological process mRNA destabilization-mediated gene silencing by miRNA RNA secondary structure unwinding transcription, DNA-templated regulation of translation RNA interference protein biosynthesis positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay positive regulation of gene expression production of miRNAs involved in gene silencing by miRNA mRNA destabilization-mediated gene silencing by siRNA positive regulation of nuclear-transcribed mRNA poly(A) tail shortening pre-miRNA processing regulation of transcription, DNA-templated positive regulation of transcription by RNA polymerase II post-embryonic development RNA phosphodiester bond hydrolysis, endonucleolytic translational initiation Wnt signaling pathway, calcium modulating pathway miRNA-mediated gene silencing by inhibition of translation negative regulation of translational initiation miRNA metabolic process production of siRNA involved in RNA interference gene silencing regulation of gene silencing by miRNA negative regulation of gene expression positive regulation of angiogenesis positive regulation of trophoblast cell migration negative regulation of amyloid precursor protein biosynthetic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 27161 239528 Ensembl ENSG00000123908 ENSMUSG00000036698 UniProt Q9UKV8 Q8CJG0 RefSeq (mRNA) NM_001164623 NM_012154 NM_153178 RefSeq (protein) NP_001158095 NP_036286 NP_694818 Location (UCSC) Chr 8: 140.52 – 140.64 Mb Chr 15: 72.97 – 73.06 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Protein argonaute-2 is a protein that in humans is encoded by the EIF2C2 gene. ^{[5] [6] [7]}

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with Dicer1 and play a role in short-interfering-RNA-mediated gene silencing. ^[7]

Interactions

EIF2C2 has been shown to interact with

• DDX20, ^{[8] [9]}
• DICER1, ^{[9] [10]}
• FMRP, FXR1P, FXR2P, ^[11] and
• TNRC6B. ^[12]

References

1. GRCh38: Ensembl release 89: ENSG00000123908 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000036698 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Koesters R, Adams V, Betts D, Moos R, Schmid M, Siermann A, Hassam S, Weitz S, Lichter P, Heitz PU, von Knebel Doeberitz M, Briner J (Dec 1999). "Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression". Genomics. 61 (2): 210–8. doi:10.1006/geno.1999.5951. PMID   10534406. S2CID   24461432.
6. Sasaki T, Shiohama A, Minoshima S, Shimizu N (Aug 2003). "Identification of eight members of the Argonaute family in the human genome small star, filled". Genomics. 82 (3): 323–30. doi:10.1016/S0888-7543(03)00129-0. PMID   12906857.
7. "Entrez Gene: EIF2C2 eukaryotic translation initiation factor 2C, 2".
8. Nelson PT, Hatzigeorgiou AG, Mourelatos Z (March 2004). "miRNP:mRNA association in polyribosomes in a human neuronal cell line". RNA. 10 (3): 387–94. doi:10.1261/rna.5181104. PMC   1370934 . PMID   14970384.
9. Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, Abel L, Rappsilber J, Mann M, Dreyfuss G (March 2002). "miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs". Genes Dev. 16 (6): 720–8. doi:10.1101/gad.974702. PMC   155365 . PMID   11914277.
10. Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K (January 2003). "Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors". Curr. Biol. 13 (1): 41–6. Bibcode:2003CBio...13...41D. doi: 10.1016/S0960-9822(02)01394-5 . PMID   12526743. S2CID   14650075.
11. Jin P, Zarnescu DC, Ceman S, Nakamoto M, Mowrey J, Jongens TA, Nelson DL, Moses K, Warren ST (February 2004). "Biochemical and genetic interaction between the fragile X mental retardation protein and the microRNA pathway". Nat. Neurosci. 7 (2): 113–7. doi:10.1038/nn1174. PMID   14703574. S2CID   1146182.
12. Till S, Lejeune E, Thermann R, Bortfeld M, Hothorn M, Enderle D, Heinrich C, Hentze MW, Ladurner AG (October 2007). "A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain". Nat. Struct. Mol. Biol. 14 (10): 897–903. doi:10.1038/nsmb1302. PMID   17891150. S2CID   8136380.

Further reading

• Carmell MA, Xuan Z, Zhang MQ, Hannon GJ (2002). "The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis". Genes Dev. 16 (21): 2733–42. doi: 10.1101/gad.1026102 . PMID   12414724.
• Mourelatos Z, Dostie J, Paushkin S, et al. (2002). "miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs". Genes Dev. 16 (6): 720–8. doi:10.1101/gad.974702. PMC   155365 . PMID   11914277.
• Martinez J, Patkaniowska A, Urlaub H, et al. (2003). "Single-stranded antisense siRNAs guide target RNA cleavage in RNAi". Cell. 110 (5): 563–74. doi:10.1016/S0092-8674(02)00908-X. hdl: 11858/00-001M-0000-0012-F2FD-2 . PMID   12230974. S2CID   10616773.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Doi N, Zenno S, Ueda R, et al. (2003). "Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors". Curr. Biol. 13 (1): 41–6. Bibcode:2003CBio...13...41D. doi: 10.1016/S0960-9822(02)01394-5 . PMID   12526743. S2CID   14650075.
• Tahbaz N, Kolb FA, Zhang H, et al. (2004). "Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer". EMBO Rep. 5 (2): 189–94. doi:10.1038/sj.embor.7400070. PMC   1298981 . PMID   14749716.
• Nelson PT, Hatzigeorgiou AG, Mourelatos Z (2004). "miRNP:mRNA association in polyribosomes in a human neuronal cell line". RNA. 10 (3): 387–94. doi:10.1261/rna.5181104. PMC   1370934 . PMID   14970384.
• Liu J, Carmell MA, Rivas FV, et al. (2004). "Argonaute2 is the catalytic engine of mammalian RNAi". Science. 305 (5689): 1437–41. Bibcode:2004Sci...305.1437L. doi: 10.1126/science.1102513 . PMID   15284456. S2CID   2778088.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Rivas FV, Tolia NH, Song JJ, et al. (2005). "Purified Argonaute2 and an siRNA form recombinant human RISC". Nat. Struct. Mol. Biol. 12 (4): 340–9. doi:10.1038/nsmb918. PMID   15800637. S2CID   2021813.
• Sen GL, Blau HM (2005). "Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies". Nat. Cell Biol. 7 (6): 633–6. doi:10.1038/ncb1265. PMID   15908945. S2CID   6085169.
• Liu J, Valencia-Sanchez MA, Hannon GJ, Parker R (2005). "MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies". Nat. Cell Biol. 7 (7): 719–23. doi:10.1038/ncb1274. PMC   1855297 . PMID   15937477.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Kraynack BA, Baker BF (2006). "Small interfering RNAs containing full 2'-O-methylribonucleotide-modified sense strands display Argonaute2/eIF2C2-dependent activity". RNA. 12 (1): 163–76. doi:10.1261/rna.2150806. PMC   1370895 . PMID   16301602.
• Maniataki E, Mourelatos Z (2006). "A human, ATP-independent, RISC assembly machine fueled by pre-miRNA". Genes Dev. 19 (24): 2979–90. doi:10.1101/gad.1384005. PMC   1315402 . PMID   16357216.
• Shi H, Tschudi C, Ullu E (2006). "Functional replacement of Trypanosoma brucei Argonaute by the human slicer Argonaute2". RNA. 12 (6): 943–7. doi:10.1261/rna.20806. PMC   1464859 . PMID   16611939.
• Wichroski MJ, Robb GB, Rana TM (2006). "Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies". PLOS Pathog. 2 (5): e41. doi: 10.1371/journal.ppat.0020041 . PMC   1458959 . PMID   16699599.