ERLIN1

ERLIN1
Identifiers
Aliases	ERLIN1 , C10orf69, Erlin-1, KE04, KEO4, SPFH1, SPG62, ER lipid raft associated 1
External IDs	OMIM: 611604; MGI: 2387613; HomoloGene: 4716; GeneCards: ERLIN1; OMA:ERLIN1 - orthologs
Gene location (Human) Chr. Chromosome 10 (human) [1] Band 10q24.31 Start 100,150,094 bp [1] End 100,186,033 bp [1]
Gene location (Mouse) Chr. Chromosome 19 (mouse) [2] Band 19|19 C3 Start 44,023,383 bp [2] End 44,058,224 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in secondary oocyte palpebral conjunctiva gingival epithelium tibia bronchial epithelial cell trabecular bone human penis liver jejunal mucosa placenta Top expressed in mucous cell of stomach spermatocyte epithelium of stomach pyloric antrum prostate jejunum cumulus cell lumbar spinal ganglion right kidney lacrimal gland More reference expression data BioGPS n/a
Gene ontology Molecular function cholesterol binding protein binding lipid binding ubiquitin protein ligase binding Cellular component integral component of membrane membrane endoplasmic reticulum endoplasmic reticulum membrane protein-containing complex Biological process SREBP signaling pathway steroid metabolic process cholesterol metabolic process negative regulation of cholesterol biosynthetic process negative regulation of fatty acid biosynthetic process lipid metabolism ubiquitin-dependent ERAD pathway transmembrane transport Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10613 226144 Ensembl ENSG00000107566 ENSMUSG00000025198 UniProt O75477 Q91X78 RefSeq (mRNA) NM_001100626 NM_001347856 NM_001347857 NM_001347858 NM_001347859 NM_001347860 NM_001347861 NM_006459 NM_001164359 NM_001164360 NM_145502 RefSeq (protein) NP_001094096 NP_001334785 NP_001334786 NP_001334787 NP_001334788 NP_001334789 NP_001334790 NP_006450 NP_001157831 NP_001157832 NP_663477 Location (UCSC) Chr 10: 100.15 – 100.19 Mb Chr 19: 44.02 – 44.06 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Erlin-1 (Endoplasmic Reticulum lipid raft-associated 1) is a protein encoded by the ERLIN1 gene in humans. ^{[5] [6] [7]} ERLIN1 and its homolog ERLIN2 are ER-localized members of the stomatin/prohibition/flotillin/HflKC (SPFH) family of proteins. They form a complex that functions to scaffold lipids and proteins. ^[8] ERLIN1 and ERLIN2 are predicted to assemble in a large ring-shaped hetero-oligomeric complex, likely formed by 24 subunits, similar to other members of the SPFH family. ^{[9] [10]}

Function

ERLIN1/2 are associated with cholesterol homeostasis. They interact with the SCAP–SREBP2–INSIG complex tightly under cholesterol repletion conditions, thus keeping this complex in the ER, where it is inactive. Silencing of ERLINs releases SREBP2 from the ER and allows it to travel to the Golgi, where it is processed and drives transcription of cholesterol synthesis genes. Activation of the SREBP2 pathway is as potent under ERLINs silencing as it is under cholesterol depletion conditions. ^[11]

References

1. GRCh38: Ensembl release 89: ENSG00000107566 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000025198 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Li N, Huang X, Zhao Z, Chen G, Zhang W, Cao X (Feb 2001). "Identification and characterization of a novel gene KE04 differentially expressed by activated human dendritic cells". Biochem Biophys Res Commun. 279 (2): 487–93. doi:10.1006/bbrc.2000.3935. PMID   11118313.
6. Browman DT, Resek ME, Zajchowski LD, Robbins SM (Jul 2006). "Erlin-1 and erlin-2 are novel members of the prohibitin family of proteins that define lipid-raft-like domains of the ER". J Cell Sci. 119 (Pt 15): 3149–60. doi: 10.1242/jcs.03060 . PMID   16835267.
7. "Entrez Gene: ERLIN1 ER lipid raft associated 1".
8. Browman, Duncan T.; Hoegg, Maja B.; Robbins, Stephen M. (August 2007). "The SPFH domain-containing proteins: more than lipid raft markers". Trends in Cell Biology. 17 (8): 394–402. doi:10.1016/j.tcb.2007.06.005.
9. Qiao, Zhu; Yokoyama, Tatsuhiko; Yan, Xin-Fu; Beh, Ing Tsyr; Shi, Jian; Basak, Sandip; Akiyama, Yoshinori; Gao, Yong-Gui (May 2022). "Cryo-EM structure of the entire FtsH-HflKC AAA protease complex". Cell Reports. 39 (9): 110890. doi:10.1016/j.celrep.2022.110890. hdl: 10356/160820 .
10. Yokoyama, Hideshi; Matsui, Ikuo (May 2023). "Higher‐order structure formation using refined monomer structures of lipid raft markers, Stomatin, Prohibitin, Flotillin, and HflK /C‐related proteins". FEBS Open Bio. 13 (5): 926–937. doi:10.1002/2211-5463.13593. PMC   10153343 .
11. Huber, Michael D.; Vesely, Paul W.; Datta, Kaustuv; Gerace, Larry (11 November 2013). "Erlins restrict SREBP activation in the ER and regulate cellular cholesterol homeostasis". Journal of Cell Biology. 203 (3): 427–436. doi:10.1083/jcb.201305076.

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Deloukas P, Earthrowl ME, Grafham DV, et al. (2004). "The DNA sequence and comparative analysis of human chromosome 10". Nature. 429 (6990): 375–81. Bibcode:2004Natur.429..375D. doi: 10.1038/nature02462 . PMID   15164054.
• Suzuki Y, Yamashita R, Shirota M, et al. (2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC   515316 . PMID   15342556.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.