Yeasts are eukaryotic,single-celled microorganisms classified as members of the fungus kingdom. The first yeast originated hundreds of millions of years ago,and at least 1,500 species are currently recognized. They are estimated to constitute 1% of all described fungal species.
Basidiomycota is one of two large divisions that,together with the Ascomycota,constitute the subkingdom Dikarya within the kingdom Fungi. Members are known as basidiomycetes. More specifically,Basidiomycota includes these groups:agarics,puffballs,stinkhorns,bracket fungi,other polypores,jelly fungi,boletes,chanterelles,earth stars,smuts,bunts,rusts,mirror yeasts,and Cryptococcus,the human pathogenic yeast.
Saccharomyces cerevisiae is a species of yeast. The species has been instrumental in winemaking,baking,and brewing since ancient times. It is believed to have been originally isolated from the skin of grapes. It is one of the most intensively studied eukaryotic model organisms in molecular and cell biology,much like Escherichia coli as the model bacterium. It is the microorganism behind the most common type of fermentation. S. cerevisiae cells are round to ovoid,5–10 μm in diameter. It reproduces by budding.
Candida albicans is an opportunistic pathogenic yeast that is a common member of the human gut flora. It can also survive outside the human body. It is detected in the gastrointestinal tract and mouth in 40–60% of healthy adults. It is usually a commensal organism,but it can become pathogenic in immunocompromised individuals under a variety of conditions. It is one of the few species of the genus Candida that cause the human infection candidiasis,which results from an overgrowth of the fungus. Candidiasis is,for example,often observed in HIV-infected patients. C. albicans is the most common fungal species isolated from biofilms either formed on (permanent) implanted medical devices or on human tissue. C. albicans,C. tropicalis,C. parapsilosis,and C. glabrata are together responsible for 50–90% of all cases of candidiasis in humans. A mortality rate of 40% has been reported for patients with systemic candidiasis due to C. albicans. By one estimate,invasive candidiasis contracted in a hospital causes 2,800 to 11,200 deaths yearly in the US. Nevertheless,these numbers may not truly reflect the true extent of damage this organism causes,given new studies indicating that C. albicans can cross the blood–brain barrier in mice.
Cryptococcus is a genus of fungi in the family Cryptococcaceae that includes both yeasts and filamentous species. The filamentous,sexual forms or teleomorphs were formerly classified in the genus Filobasidiella,while Cryptococcus was reserved for the yeasts. Most yeast species formerly referred to Cryptococcus have now been placed in different genera. The name Cryptococcus comes from the Greek for "hidden sphere". Some Cryptococcus species cause a disease called cryptococcosis.
Cryptococcus neoformans is an encapsulated yeast belonging to the class Tremellomycetes and an obligate aerobe that can live in both plants and animals. Its teleomorph is a filamentous fungus,formerly referred to Filobasidiella neoformans. In its yeast state,it is often found in bird excrement. Cryptococcus neoformans can cause disease in apparently immunocompetent,as well as immunocompromised,hosts.
The yeast Saccharomyces cerevisiae is a simple single-celled eukaryote with both a diploid and haploid mode of existence. The mating of yeast only occurs between haploids,which can be either the a or α(alpha) mating type and thus display simple sexual differentiation. Mating type is determined by a single locus,MAT,which in turn governs the sexual behaviour of both haploid and diploid cells. Through a form of genetic recombination,haploid yeast can switch mating type as often as every cell cycle.
Fungi are a diverse group of organisms that employ a huge variety of reproductive strategies,ranging from fully asexual to almost exclusively sexual species. Most species can reproduce both sexually and asexually,alternating between haploid and diploid forms. This contrasts with most multicellular eukaryotes such as mammals,where the adults are usually diploid and produce haploid gametes which combine to form the next generation. In fungi,both haploid and diploid forms can reproduce –haploid individuals can undergo asexual reproduction while diploid forms can produce gametes that combine to give rise to the next generation.
In enzymology,a sterol 14-demethylase (EC 1.14.13.70) is an enzyme of the cytochrome P450 (CYP) superfamily. It is any member of the CYP51 family. It catalyzes a chemical reaction such as:
In enzymology,a ferric-chelate reductase (EC 1.16.1.7) is an enzyme that catalyzes the chemical reaction
Pathogenic fungi are fungi that cause disease in humans or other organisms. Although fungi are eukaryotic,many pathogenic fungi are microorganisms. Approximately 300 fungi are known to be pathogenic to humans;their study is called "medical mycology". Fungal infections are estimated to kill more people than either tuberculosis or malaria—about two million people per year.
A killer yeast is a yeast,such as Saccharomyces cerevisiae,which is able to secrete one of a number of toxic proteins which are lethal to susceptible cells. These "killer toxins" are polypeptides that kill sensitive cells of the same or related species,often functioning by creating pores in target cell membranes. These yeast cells are immune to the toxic effects of the protein due to an intrinsic immunity. Killer yeast strains can be a problem in commercial processing because they can kill desirable strains. The killer yeast system was first described in 1963. Study of killer toxins helped to better understand the secretion pathway of yeast,which is similar to those of more complex eukaryotes. It also can be used in treatment of some diseases,mainly those caused by fungi.
A fungus is any member of the group of eukaryotic organisms that includes microorganisms such as yeasts and molds,as well as the more familiar mushrooms. These organisms are classified as one of the traditional eukaryotic kingdoms,along with Animalia,Plantae and either Protista or Protozoa and Chromista.
The meiotic recombination checkpoint monitors meiotic recombination during meiosis,and blocks the entry into metaphase I if recombination is not efficiently processed.
The bZIP intron RNA motif is an RNA structure guiding splicing of a non-canonical intron from bZIP-containing genes called HAC1 in yeast,XBP1 in Metazoa,Hxl1 or Cib1 in Basidiomycota and bZIP60 in plants. Splicing is performed independently of the spliceosome by Ire1,a kinase with endoribonuclease activity. Exons are joined by a tRNA ligase. Recognition of the intron splice sites is mediated by a base-paired secondary structure of the mRNA that forms at the exon/intron boundaries. Splicing of the bZIP intron is a key regulatory step in the unfolded protein response (UPR). The Ire-mediated unconventional splicing was first described for HAC1 in S. cerevisiae.
Vomocytosis is the cellular process by phagocytes expel live organisms that they have engulfed without destroying the organism. Vomocytosis is one of many methods used by cells to expel internal materials into their external environment,yet it is distinct in that both the engulfed organism and host cell remain undamaged by expulsion. As engulfed organisms are released without being destroyed,vomocytosis has been hypothesized to be utilized by pathogens as an escape mechanism from the immune system. The exact mechanisms,as well as the repertoire of cells that utilize this mechanism,are currently unknown,yet interest in this unique cellular process is driving continued research with the hopes of elucidating these unknowns.
Arturo Casadevall is a Bloomberg Distinguished Professor of Molecular Microbiology &Immunology and Infectious Diseases at the Johns Hopkins Bloomberg School of Public Health and Johns Hopkins School of Medicine,and the Alfred and Jill Sommer Professor and Chair of the W. Harry Feinstone Department of Molecular Microbiology and Immunology at the Johns Hopkins Bloomberg School of Public Health. He is an internationally recognized expert in infectious disease research,with a focus on fungal and bacterial pathogenesis and basic immunology of antibody structure-function. He was elected a member of the National Academy of Sciences in 2022.
The Gal4 transcription factor is a positive regulator of gene expression of galactose-induced genes. This protein represents a large fungal family of transcription factors,Gal4 family,which includes over 50 members in the yeast Saccharomyces cerevisiae e.g. Oaf1,Pip2,Pdr1,Pdr3,Leu3.
Kaustuv Sanyal is an Indian molecular biologist,mycologist and a professor at the Molecular Biology and Genetics Unit of the Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR). He is known for his molecular and genetic studies of pathogenic yeasts such as Candida and Cryptococcus). An alumnus of Bidhan Chandra Krishi Viswavidyalaya and Madurai Kamaraj University from where he earned a BSc in agriculture and MSc in biotechnology respectively,Sanyal did his doctoral studies at Bose Institute to secure a PhD in Yeast genetics. He moved to the University of California,Santa Barbara,USA to work in the laboratory of John Carbon on the discovery of centromeres in Candida albicans. He joined JNCASR in 2005. He is a member of the Faculty of 1000 in the disciplines of Microbial Evolution and Genomics and has delivered invited speeches which include the Gordon Research Conference,EMBO conferences on comparative genomics and kinetochores. The Department of Biotechnology of the Government of India awarded him the National Bioscience Award for Career Development,one of the highest Indian science awards,for his contributions to biosciences,in 2012. He has also been awarded with the prestigious Tata Innovation Fellowship in 2017. The National Academy of Sciences,India elected him as a fellow in 2014. He is also an elected fellow of Indian Academy of Sciences (2017),and the Indian National Science Academy (2018). In 2019,he has been elected to Fellowship in the American Academy of Microbiology (AAM),the honorific leadership group within the American Society for Microbiology.
