KIF3B

KIF3B
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3B6U
Identifiers
Aliases	KIF3B , FLA8, HH0048, KLP-11, kinesin family member 3B, RP89
External IDs	OMIM: 603754; MGI: 107688; HomoloGene: 55849; GeneCards: KIF3B; OMA:KIF3B - orthologs
Gene location (Human) Chr. Chromosome 20 (human) [1] Band 20q11.21 Start 32,277,651 bp [1] End 32,335,011 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2 H1|2 75.41 cM Start 153,133,333 bp [2] End 153,175,310 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in middle temporal gyrus islet of Langerhans sperm bronchial epithelial cell renal medulla jejunal mucosa secondary oocyte Brodmann area 23 parietal lobe superior frontal gyrus Top expressed in spermatocyte spermatid seminiferous tubule granulocyte neural layer of retina lumbar spinal ganglion dentate gyrus of hippocampal formation granule cell retinal pigment epithelium primary visual cortex superior frontal gyrus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding microtubule binding protein binding plus-end-directed microtubule motor activity ATP binding microtubule motor activity ATPase activity intraciliary transport particle B binding Cellular component cytoplasm intraciliary transport particle cytosol membrane microtubule cytoskeleton spindle plus-end kinesin complex midbody ciliary tip spindle microtubule extracellular exosome cytoskeleton microtubule axon cytoplasm centrosome kinesin II complex cell projection axon cilium kinesin complex Biological process plus-end-directed vesicle transport along microtubule antigen processing and presentation of exogenous peptide antigen via MHC class II mitotic spindle organization anterograde axonal transport positive regulation of cytokinesis mitotic spindle assembly determination of left/right symmetry mitotic centrosome separation retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum microtubule-based movement intraciliary transport involved in cilium assembly Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9371 16569 Ensembl ENSG00000101350 ENSMUSG00000027475 UniProt O15066 Q61771 RefSeq (mRNA) NM_004798 NM_008444 RefSeq (protein) NP_004789 NP_032470 Location (UCSC) Chr 20: 32.28 – 32.34 Mb Chr 2: 153.13 – 153.18 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Kinesin-like protein KIF3B is a protein that in humans is encoded by the KIF3B gene. ^{[5] [6]} KIF3B is an N-type protein that complexes with two other kinesin proteins to form two-headed anterograde motors. ^[7] First, KIF3B forms a heterodimer with KIF3A (kinesin-like protein KIF3A); (KIF3A/3B), that is membrane-bound and has ATPase activity. Then KIFAP3 (KAP3, kinesin superfamily associated protein–3) binds to the tail domain to form a heterotrimeric motor. ^[7] This motor has a plus end-directed microtubule sliding activity that exhibits a velocity of ~0.3 μm/s a. ^[8] There are 14 kinesin protein families in the kinesin superfamily and KIF3B is part of the Kinesin-2 family, of kinesins that can all form heterotrimeric complexes. ^[9] Expression of the three motor subunits is ubiquitous. The KIG3A/3B/KAP3 motors can transport 90 to 160 nm in diameter organelles. ^[7]

There are many orthologous KIF3B genes that are expressed in Drosophila , the sea urchin, Bos taurus , Canis familiaris , Equus caballus , Felis catus , Macaca mulatta , Mus musculus , Pan troglodytes , and Rattus norvegicus .

Function

The heterotrimeric KIF3B/KIF3A/KAP3 motor machinery functions in the intracellular transport of multiple different molecules such as β-catenin and MT1-MMP. ^{[10] [11]} KIF3B activity has been implicated with various cellular processes such as intracellular movement of organelles, intraflagellar transport, chromosome movement during mitosis and meiosis, and cellular interaction with the extracellular matrix. ^{[6] [12] [13]}

KIF3B also regulates the interaction of cancer cells with the extracellular matrix (ECM), in particular the transport of MT1-MMP to the cancer cell front is essential for collagen fiber matrix realignment and degradation. ^{[14] [15]}

Interactions

KIF3B has been shown to interact with the SMC3 subunit of the cohesin complex and with RAB4A. ^[16]

References

1. GRCh38: Ensembl release 89: ENSG00000101350 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000027475 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nagase T, Ishikawa K, Nakajima D, Ohira M, Seki N, Miyajima N, et al. (April 1997). "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Research. 4 (2): 141–50. doi: 10.1093/dnares/4.2.141 . PMID   9205841.
6. "Entrez Gene: KIF3B kinesin family member 3B".
7. Hirokawa N (January 1998). "Kinesin and dynein superfamily proteins and the mechanism of organelle transport". Science. 279 (5350): 519–26. Bibcode:1998Sci...279..519H. doi:10.1126/science.279.5350.519. PMID   9438838.
8. Hirokawa N (January 1998). "Kinesin and dynein superfamily proteins and the mechanism of organelle transport". Science. 279 (5350): 519–26. Bibcode:1998Sci...279..519H. doi:10.1126/science.279.5350.519. PMID   9438838.
9. Lawrence CJ, Dawe RK, Christie KR, Cleveland DW, Dawson SC, Endow SA, et al. (October 2004). "A standardized kinesin nomenclature". The Journal of Cell Biology. 167 (1): 19–22. doi:10.1083/jcb.200408113. PMC   2041940 . PMID   15479732.
10. Jimbo T, Kawasaki Y, Koyama R, Sato R, Takada S, Haraguchi K, Akiyama T (April 2002). "Identification of a link between the tumour suppressor APC and the kinesin superfamily". Nature Cell Biology. 4 (4): 323–7. doi:10.1038/ncb779. PMID   11912492. S2CID   10745049.
11. Wiesner C, Faix J, Himmel M, Bentzien F, Linder S (September 2010). "KIF5B and KIF3A/KIF3B kinesins drive MT1-MMP surface exposure, CD44 shedding, and extracellular matrix degradation in primary macrophages". Blood. 116 (9): 1559–69. doi: 10.1182/blood-2009-12-257089 . PMID   20505159.
12. Scholey JM (April 1996). "Kinesin-II, a membrane traffic motor in axons, axonemes, and spindles". The Journal of Cell Biology. 133 (1): 1–4. doi:10.1083/jcb.133.1.1. PMC   2120781 . PMID   8601599.
13. Lawrence CJ, Dawe RK, Christie KR, Cleveland DW, Dawson SC, Endow SA, et al. (October 2004). "A standardized kinesin nomenclature". The Journal of Cell Biology. 167 (1): 19–22. doi:10.1083/jcb.200408113. PMC   2041940 . PMID   15479732.
14. Kravtsov O, Hartley CP, Compérat EM, Iczkowski KA (2019). "KIF3B protein expression loss correlates with metastatic ability of prostate cancer". American Journal of Clinical and Experimental Urology. 7 (3): 178–181. PMC   6627541 . PMID   31317057.
15. Stoletov K, Willetts L, Paproski RJ, Bond DJ, Raha S, Jovel J, et al. (June 2018). "Quantitative in vivo whole genome motility screen reveals novel therapeutic targets to block cancer metastasis". Nature Communications. 9 (1): 2343. Bibcode:2018NatCo...9.2343S. doi: 10.1038/s41467-018-04743-2 . PMC   6002534 . PMID   29904055.
16. Imamura T, Huang J, Usui I, Satoh H, Bever J, Olefsky JM (July 2003). "Insulin-induced GLUT4 translocation involves protein kinase C-lambda-mediated functional coupling between Rab4 and the motor protein kinesin". Molecular and Cellular Biology. 23 (14): 4892–900. doi:10.1128/MCB.23.14.4892-4900.2003. PMC   162221 . PMID   12832475.

Further reading

• Yamazaki H, Nakata T, Okada Y, Hirokawa N (September 1995). "KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport". The Journal of Cell Biology. 130 (6): 1387–99. doi:10.1083/jcb.130.6.1387. PMC   2120571 . PMID   7559760.
• Nagata K, Puls A, Futter C, Aspenstrom P, Schaefer E, Nakata T, et al. (January 1998). "The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3". The EMBO Journal. 17 (1): 149–58. doi:10.1093/emboj/17.1.149. PMC   1170366 . PMID   9427749.
• Shimizu K, Shirataki H, Honda T, Minami S, Takai Y (March 1998). "Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide". The Journal of Biological Chemistry. 273 (12): 6591–4. doi: 10.1074/jbc.273.12.6591 . PMID   9506951.
• Nonaka S, Tanaka Y, Okada Y, Takeda S, Harada A, Kanai Y, et al. (December 1998). "Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein". Cell. 95 (6): 829–37. doi: 10.1016/S0092-8674(00)81705-5 . PMID   9865700. S2CID   62805329.
• Navarro E, Espinosa L, Adell T, Torà M, Berrozpe G, Real FX (July 1999). "Expressed sequence tag (EST) phenotyping of HT-29 cells: cloning of ser/thr protein kinase EMK1, kinesin KIF3B, and of transcripts that include Alu repeated elements". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1450 (3): 254–64. doi:10.1016/S0167-4889(99)00051-8. PMID   10395937.
• Whitehead JL, Wang SY, Bost-Usinger L, Hoang E, Frazer KA, Burnside B (November 1999). "Photoreceptor localization of the KIF3A and KIF3B subunits of the heterotrimeric microtubule motor kinesin II in vertebrate retina". Experimental Eye Research. 69 (5): 491–503. doi:10.1006/exer.1999.0724. PMID   10548469.
• Jimbo T, Kawasaki Y, Koyama R, Sato R, Takada S, Haraguchi K, Akiyama T (April 2002). "Identification of a link between the tumour suppressor APC and the kinesin superfamily". Nature Cell Biology. 4 (4): 323–7. doi:10.1038/ncb779. PMID   11912492. S2CID   10745049.
• Baker SA, Freeman K, Luby-Phelps K, Pazour GJ, Besharse JC (September 2003). "IFT20 links kinesin II with a mammalian intraflagellar transport complex that is conserved in motile flagella and sensory cilia". The Journal of Biological Chemistry. 278 (36): 34211–8. doi: 10.1074/jbc.M300156200 . PMID   12821668.
• Imamura T, Huang J, Usui I, Satoh H, Bever J, Olefsky JM (July 2003). "Insulin-induced GLUT4 translocation involves protein kinase C-lambda-mediated functional coupling between Rab4 and the motor protein kinesin". Molecular and Cellular Biology. 23 (14): 4892–900. doi:10.1128/MCB.23.14.4892-4900.2003. PMC   162221 . PMID   12832475.
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, et al. (July 2004). "Functional proteomics mapping of a human signaling pathway". Genome Research. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC   442148 . PMID   15231748.
• Haraguchi K, Hayashi T, Jimbo T, Yamamoto T, Akiyama T (February 2006). "Role of the kinesin-2 family protein, KIF3, during mitosis". The Journal of Biological Chemistry. 281 (7): 4094–9. doi: 10.1074/jbc.M507028200 . PMID   16298999.
• Wu Y, Dai XQ, Li Q, Chen CX, Mai W, Hussain Z, et al. (November 2006). "Kinesin-2 mediates physical and functional interactions between polycystin-2 and fibrocystin". Human Molecular Genetics. 15 (22): 3280–92. doi: 10.1093/hmg/ddl404 . PMID   17008358.