A
- Abepalpus Townsend, 1931
- Acantholespesia Wood, 1987
- Acaulona van der Wulp, 1884
- Acemya Robineau-Desvoidy, 1830 [2]
- Acroceronia Cortés, 1951
- Acronacantha van der Wulp, 1891
- Actia Robineau-Desvoidy, 1830 [2]
- Actinochaeta Brauer & von Bergenstamm, 1889
- Actinochaetopteryx Townsend, 1927 [3]
- Actinodoria Townsend, 1927 [3]
- Actinominthella Townsend, 1928
- Actinoplagia Blanchard, 1940
- Acuphoceropsis Blanchard, 1943
- Adejeania Townsend, 1913
- Admontia Brauer & von Bergenstamm, 1889
- Aesia Richter, 2011 [4]
- Afrolixa Curran, 1939
- Afrophylax Cerretti & O’Hara in O’Hara & Cerretti, 2016 [5]
- Agaedioxenis Villeneuve, 1939
- Agicuphocera Townsend, 1915
- Aglummyia Townsend, 1912
- Aldrichiopa Guimarães, 1971
- Aldrichomyia Özdikmen, 2006
- Alexogloblinia Cortés, 1945
- Allelomyia González, 1992
- Allophorocera Hendel, 1901
- Alloprosopaea Villeneuve, 1923
- Allosturmia Blanchard, 1958
- Allothelaira Villeneuve, 1915
- Alpinoplagia Townsend, 1931
- Alsomyia Brauer & von Bergenstamm, 1891
- Alsopsyche Brauer & von Bergenstamm, 1891
- Altaia Malloch, 1938
- Amazohoughia Townsend, 1934
- Amblychaeta Aldrich, 1934
- Amelibaea Mesnil, 1955
- Amesiomima Mesnil, 1950
- Ametadoria Townsend, 1927 [3]
- Amicrotrichomma Townsend, 1927 [3]
- Amnonia Kugler, 1971
- Amphibolia Macquart, 1843
- Amphicestonia Villeneuve, 1939
- Amphitropesa Townsend, 1933
- Anacamptomyia Bischof, 1904
- Anadiscalia Curran, 1934
- Anadistichona Townsend, 1934
- Anaeudora Townsend, 1933
- Anagonia Brauer & von Bergenstamm, 1891
- Anamastax Brauer & von Bergenstamm, 1891
- Anametopochaeta Townsend, 1919
- Ancistrophora Schiner, 1865
- Andesimyia Brèthes, 1909
- Andinomyia Townsend, 1912
- Androsoma Cortés & Campos, 1971
- Anechuromyia Mesnil & Shima, 1979
- Anemorilla Townsend, 1915
- Aneogmena Brauer & von Bergenstamm, 1891
- Anepalpus Townsend, 1931
- Angustia Sellers, 1943
- Anisia van der Wulp, 1890
- Anomalostomyia Cerretti & Barraclough, 2007
- Anoxynops Townsend, 1927 [3]
- Anthomyiopsis Townsend, 1916
- Antistasea Bischof, 1904
- Antistaseopsis Townsend, 1934
- Anurophylla Villeneuve, 1938
- Apalpostoma Malloch, 1930
- Apatemyia Macquart, 1846
- Aphria Robineau-Desvoidy, 1830 [2]
- Aplomya Robineau-Desvoidy, 1830 [2]
- Aplomyodoria Townsend, 1928
- Aplomyopsis Townsend, 1927 [3]
- Apomorphomyia Crosskey, 1984
- Aporeomyia Pape & Shima, 1993
- Aprotheca Macquart, 1851
- Arama Richter, 1972
- Araucogonia Cortés, 1976
- Araucosimus Aldrich, 1934
- Archytas Jaennicke, 1867
- Archytoepalpus Townsend, 1927 [3]
- Arcona Richter, 1988
- Arctosoma Aldrich, 1934
- Argyrochaetona Townsend, 1919
- Argyromima Brauer & von Bergenstamm, 1889
- Argyrophylax Brauer & von Bergenstamm, 1889
- Argyrothelaira Townsend, 1916
- Aridalia Curran, 1934
- Arrhenomyza Malloch, 1929
- Arrhinactia Townsend, 1927 [3]
- Asetulia Malloch, 1938
- Asilidotachina Townsend, 1931
- Asseclamyia Reinhard, 1956
- Atacta Schiner, 1868
- Atactopsis Townsend, 1917
- Atactosturmia Townsend, 1915
- Ateloglossa Coquillett, 1899
- Ateloglutus Aldrich, 1934
- Athrycia Robineau-Desvoidy, 1830 [2]
- Atlantomyia Crosskey, 1977
- Atractocerops Townsend, 1916
- Atrichiopoda Townsend, 1931
- Atylomyia Brauer, 1898
- Atylostoma Brauer & von Bergenstamm, 1889
- Aulacephala Macquart, 1851
- Austeniops Townsend, 1915
- Australotachina Curran, 1938
- Austromacquartia Townsend, 1934
- Austronilea Crosskey, 1967
- Austrophasiopsis Townsend, 1933
- Austrophorocera Townsend, 1916
- Austrophryno Townsend, 1916
- Austrophytomyptera Blanchard, 1962
- Austrosolieria Cerretti & O’Hara in O’Hara & Cerretti, 2016 [5]
- Avibrissia Malloch, 1932
- Avibrissina Malloch, 1932
- Avibrissosturmia Townsend, 1927 [3]
- Azygobothria Townsend, 1911
