ME1 (gene)

ME1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2AW5, 3WJA
Identifiers
Aliases	ME1 , HUMNDME, MES, malic enzyme 1
External IDs	OMIM: 154250 MGI: 97043 HomoloGene: 134785 GeneCards: ME1
EC number	1.1.1.40
Gene location (Human) Chr. Chromosome 6 (human) [1] Band 6q14.2 Start 83,210,402 bp [1] End 83,431,051 bp [1]
Gene location (Mouse) Chr. Chromosome 9 (mouse) [2] Band 9 E3.1|9 46.58 cM Start 86,463,424 bp [2] End 86,578,006 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in spinal ganglia trigeminal ganglion vulva gums external globus pallidus jejunal mucosa Left adrenal gland cavity of mouth superior vestibular nucleus secondary oocyte Top expressed in adrenal gland mucous cell of stomach pyloric antrum Epithelium of stomach subcutaneous adipose tissue brown adipose tissue esophagus white adipose tissue extensor digitorum longus muscle lacrimal gland More reference expression data BioGPS More reference expression data
Gene ontology Molecular function manganese ion binding ADP binding NAD binding metal ion binding NADP binding electron transfer activity malate dehydrogenase (decarboxylating) (NAD+) activity oxidoreductase activity oxaloacetate decarboxylase activity malic enzyme activity malate dehydrogenase (decarboxylating) (NADP+) activity Cellular component cytoplasm cytosol mitochondrion Biological process regulation of NADP metabolic process protein tetramerization NADP biosynthetic process response to hormone response to carbohydrate malate metabolic process regulation of lipid metabolic process carbohydrate metabolic process electron transport chain pyruvate metabolic process nucleotide biosynthetic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 4199 17436 Ensembl ENSG00000065833 ENSMUSG00000032418 UniProt P48163 P06801 RefSeq (mRNA) NM_002395 NM_001198933 NM_008615 RefSeq (protein) NP_002386 NP_001185862 NP_032641 Location (UCSC) Chr 6: 83.21 – 83.43 Mb Chr 9: 86.46 – 86.58 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

NADP-dependent malic enzyme is a protein that in humans is encoded by the ME1 gene. ^{[5] [6]}

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate to pyruvate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. ^[6]

References

1. GRCh38: Ensembl release 89: ENSG00000065833 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000032418 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Loeber G, Dworkin MB, Infante A, Ahorn H (Jun 1994). "Characterization of cytosolic malic enzyme in human tumor cells". FEBS Lett. 344 (2–3): 181–6. doi:10.1016/0014-5793(94)00386-6. PMID   8187880. S2CID   41426196.
6. "Entrez Gene: ME1 malic enzyme 1, NADP(+)-dependent, cytosolic".

Further reading

• Povey S, Wilson DE, Harris H, et al. (1978). "Sub-unit structure of soluble and mitochondrial malic enzyme: demonstration of human mitochondrial enzyme in human-mouse hybrids". Ann. Hum. Genet. 39 (2): 203–12. doi:10.1111/j.1469-1809.1975.tb00123.x. PMID   1088824. S2CID   39275324.
• Tessarolo D, Liguori M, Giacanelli M (1991). "Human malic enzymes in heart and muscle: evidence of a selective distribution". Biochem. Med. Metab. Biol. 45 (1): 1–5. doi:10.1016/0885-4505(91)90002-3. PMID   2015102.
• Taroni F, Di Donato S (1988). "Purification and properties of cytosolic malic enzyme from human skeletal muscle". Int. J. Biochem. 20 (8): 857–66. doi:10.1016/0020-711X(88)90075-4. PMID   3169368.
• Chen TR, McMorris FA, Creagan R, et al. (1973). "Assignment of the genes for malate oxidoreductase decarboxylating to chromosome 6 and peptidase B and lactate dehydrogenase B to chromosome 12 in man". Am. J. Hum. Genet. 25 (2): 200–7. PMC   1762508 . PMID   4689040.
• Bukato G, Kochan Z, Swierczyński J (1996). "Different regulatory properties of the cytosolic and mitochondrial forms of malic enzyme isolated from human brain". Int. J. Biochem. Cell Biol. 27 (10): 1003–8. doi:10.1016/1357-2725(95)00080-9. PMID   7496989.
• González-Manchón C, Ferrer M, Ayuso MS, Parrilla R (1995). "Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver". Gene. 159 (2): 255–60. doi:10.1016/0378-1119(95)00004-P. PMID   7622060.
• Bukato G, Kochan Z, Swierczyński J (1995). "Purification and properties of cytosolic and mitochondrial malic enzyme isolated from human brain". Int. J. Biochem. Cell Biol. 27 (1): 47–54. doi:10.1016/1357-2725(94)00057-3. PMID   7757881.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Chou WY, Huang SM, Chang GG (1996). "Nonidentity of the cDNA sequence of human breast cancer cell malic enzyme to that from the normal human cell". J. Protein Chem. 15 (3): 273–9. doi:10.1007/BF01887116. PMID   8804575. S2CID   31679272.
• González-Manchón C, Butta N, Ferrer M, et al. (1997). "Molecular cloning and functional characterization of the human cytosolic malic enzyme promoter: thyroid hormone responsiveness". DNA Cell Biol. 16 (5): 533–44. doi:10.1089/dna.1997.16.533. PMID   9174159.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Sylvius N, Tesson F, Gayet C, et al. (2001). "A new locus for autosomal dominant dilated cardiomyopathy identified on chromosome 6q12-q16". Am. J. Hum. Genet. 68 (1): 241–6. doi:10.1086/316929. PMC   1234920 . PMID   11085912.
• Butta N, González-Manchón C, Arias-Salgado EG, et al. (2001). "Cloning and functional characterization of the 5' flanking region of the human mitochondrial malic enzyme gene. Regulatory role of Sp1 and AP-2". Eur. J. Biochem. 268 (10): 3017–27. doi:10.1046/j.1432-1327.2001.02194.x. hdl: 10261/73615 . PMID   11358520.
• Yang Z, Lanks CW, Tong L (2002). "Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate". Structure. 10 (7): 951–60. doi: 10.1016/S0969-2126(02)00788-8 . PMID   12121650.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Mungall AJ, Palmer SA, Sims SK, et al. (2003). "The DNA sequence and analysis of human chromosome 6". Nature. 425 (6960): 805–11. Bibcode:2003Natur.425..805M. doi: 10.1038/nature02055 . PMID   14574404.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Hsieh JY, Liu GY, Chang GG, Hung HC (2006). "Determinants of the dual cofactor specificity and substrate cooperativity of the human mitochondrial NAD(P)+-dependent malic enzyme: functional roles of glutamine 362". J. Biol. Chem. 281 (32): 23237–45. doi: 10.1074/jbc.M603451200 . PMID   16757477.