MSH4

Last updated
MSH4
Identifiers
Aliases MSH4 , mutS homolog 4
External IDs OMIM: 602105; MGI: 1860077; HomoloGene: 1830; GeneCards: MSH4; OMA:MSH4 - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_002440

NM_001282054
NM_031870

RefSeq (protein)

NP_002431

NP_001268983
NP_114076

Location (UCSC) Chr 1: 75.8 – 75.91 Mb Chr 3: 153.56 – 153.61 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

MutS protein homolog 4 is a protein that in humans is encoded by the MSH4 gene. [5] [6]

Contents

Function

The MSH4 and MSH5 proteins form a hetero-oligomeric structure (heterodimer) in yeast and humans. [7] [8] [9] In the yeast Saccharomyces cerevisiae MSH4 and MSH5 act specifically to facilitate crossovers between homologous chromosomes during meiosis. [7] The MSH4/MSH5 complex binds and stabilizes double Holliday junctions and promotes their resolution into crossover products. An MSH4 hypomorphic (partially functional) mutant of S. cerevisiae showed a 30% genome wide reduction in crossover numbers, and a large number of meioses with non exchange chromosomes. [10] Nevertheless this mutant gave rise to spore viability patterns suggesting that segregation of non-exchange chromosomes occurred efficiently. Thus, in S. cerevisiae, proper segregation apparently does not entirely depend on crossovers between homologous pairs.

The him-14 gene of the worm Caenorhabditis elegans encodes an ortholog of MSH4. [11] Formation of crossovers during C. elegans meiosis requires the him-14(MSH4) gene. Loss of him-14(MSH-4) function severely reduces crossing over, resulting in lack of chiasmata between homologs and consequent missegregation. Thus, in C. elegans, segregation apparently does depend on crossovers between homologous pairs. Him-14(MSH4) functions during the pachytene stage of meiosis, indicating that it is not needed for establishing the preceding stages of pairing and synapsis of homologous chromosomes.

In an MSH4 mutant of rice, chiasma frequency was dramatically decreased to about 10% of the wild-type frequency, although the synaptonemal complex was normally installed. [12] It is likely that MSH4 interacts with MSH5 to promote the majority of crossovers during rice meiosis.

In general it appears that MSH4 acts during meiosis to direct the recombinational repair of some DNA double-strand breaks towards the crossover option rather than the non-cross over option (see Homologous recombination).

Interactions

MSH4 has been shown to interact with MLH1, [13] MSH5 [8] [9] [14] and MLH3. [15]

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000057468 Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000005493 Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Paquis-Flucklinger V, Santucci-Darmanin S, Paul R, Saunières A, Turc-Carel C, Desnuelle C (Sep 1997). "Cloning and expression analysis of a meiosis-specific MutS homolog: the human MSH4 gene". Genomics. 44 (2): 188–94. doi:10.1006/geno.1997.4857. PMID   9299235.
  6. "Entrez Gene: MSH4 mutS homolog 4 (E. coli)".
  7. 1 2 Pochart P, Woltering D, Hollingsworth NM (1997). "Conserved properties between functionally distinct MutS homologs in yeast". J. Biol. Chem. 272 (48): 30345–9. doi: 10.1074/jbc.272.48.30345 . PMID   9374523.
  8. 1 2 Winand NJ, Panzer JA, Kolodner RD (1998). "Cloning and characterization of the human and Caenorhabditis elegans homologs of the Saccharomyces cerevisiae MSH5 gene". Genomics. 53 (1): 69–80. doi: 10.1006/geno.1998.5447 . PMID   9787078.
  9. 1 2 Bocker T, Barusevicius A, Snowden T, Rasio D, Guerrette S, Robbins D, Schmidt C, Burczak J, Croce CM, Copeland T, Kovatich AJ, Fishel R (1999). "hMSH5: a human MutS homologue that forms a novel heterodimer with hMSH4 and is expressed during spermatogenesis". Cancer Res. 59 (4): 816–22. PMID   10029069.
  10. Krishnaprasad GN, Anand MT, Lin G, Tekkedil MM, Steinmetz LM, Nishant KT (2015). "Variation in crossover frequencies perturb crossover assurance without affecting meiotic chromosome segregation in Saccharomyces cerevisiae". Genetics. 199 (2): 399–412. doi:10.1534/genetics.114.172320. PMC   4317650 . PMID   25467183.
  11. Zalevsky J, MacQueen AJ, Duffy JB, Kemphues KJ, Villeneuve AM (1999). "Crossing over during Caenorhabditis elegans meiosis requires a conserved MutS-based pathway that is partially dispensable in budding yeast". Genetics. 153 (3): 1271–83. doi:10.1093/genetics/153.3.1271. PMC   1460811 . PMID   10545458.
  12. Zhang L, Tang D, Luo Q, Chen X, Wang H, Li Y, Cheng Z (2014). "Crossover formation during rice meiosis relies on interaction of OsMSH4 and OsMSH5". Genetics. 198 (4): 1447–56. doi:10.1534/genetics.114.168732. PMC   4256764 . PMID   25278554.
  13. Santucci-Darmanin S, Walpita D, Lespinasse F, Desnuelle C, Ashley T, Paquis-Flucklinger V (Aug 2000). "MSH4 acts in conjunction with MLH1 during mammalian meiosis". FASEB Journal. 14 (11): 1539–47. doi: 10.1096/fj.14.11.1539 . PMID   10928988.
  14. Her C, Wu X, Griswold MD, Zhou F (Feb 2003). "Human MutS homologue MSH4 physically interacts with von Hippel-Lindau tumor suppressor-binding protein 1". Cancer Research. 63 (4): 865–72. PMID   12591739.
  15. Santucci-Darmanin S, Neyton S, Lespinasse F, Saunières A, Gaudray P, Paquis-Flucklinger V (Jul 2002). "The DNA mismatch-repair MLH3 protein interacts with MSH4 in meiotic cells, supporting a role for this MutL homolog in mammalian meiotic recombination". Human Molecular Genetics. 11 (15): 1697–706. CiteSeerX   10.1.1.586.4478 . doi:10.1093/hmg/11.15.1697. PMID   12095912.

Further reading