Mastitis in dairy cattle

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Gangrenous mastitis in a cow after 10 days. Green arrow indicates complete necrosis of the teat. Yellow arrows indicate the limits of the gangrenous tissue, but the necrotic area is not well delimited on the upper part of the udder. Mamite a grangrin vatche pes fritches.jpg
Gangrenous mastitis in a cow after 10 days. Green arrow indicates complete necrosis of the teat. Yellow arrows indicate the limits of the gangrenous tissue, but the necrotic area is not well delimited on the upper part of the udder.
Dairy cow with gangrenous mastitis (rear quarter) Cow Gangrenous mastitis.jpg
Dairy cow with gangrenous mastitis (rear quarter)

Bovine mastitis is the persistent, inflammatory reaction of the udder tissue due to physical trauma or microorganisms infections. Mastitis, a potentially fatal mammary gland infection, is the most common disease in dairy cattle in the United States and worldwide. It is also the most costly disease to the dairy industry. [1] Milk from cows suffering from mastitis has an increased somatic cell count. Prevention and control of mastitis requires consistency in sanitizing the cow barn facilities, proper milking procedure and segregation of infected animals. Treatment of the disease is carried out by penicillin injection in combination with sulphar drug.

Contents

Definition

Mastitis occurs when white blood cells (leukocytes) are released into the mammary gland, usually in response to bacteria invading the teat canal or occasionally by chemical, mechanical, or thermal trauma on the udder. Milk-secreting tissue and various ducts throughout the mammary gland are damaged due to toxins released by the bacteria resulting in reduced milk yield and quality.

Identification

The quarter with gangrenous mastitis Gangrenous teat.jpg
The quarter with gangrenous mastitis
A gangrened udder (which sloughed naturally) Mamite grangrin moirt pes.JPG
A gangrened udder (which sloughed naturally)

This disease can be identified by abnormalities in the udder such as swelling, heat, redness, hardness, or pain (if it is clinical). Other indications of mastitis may be abnormalities in milk such as a watery appearance, flakes, or clots. When infected with sub-clinical mastitis, a cow does not show any visible signs of infection or abnormalities in milk or on the udder. [1]

Mastitis-causing bacteria

Bacterial cells of Staphylococcus aureus, one of the causal agents of mastitis in dairy cows. Its large capsule protects the organism from attack by the cow's immunological defenses. Staphylococcus aureus, 50,000x, USDA, ARS, EMU.jpg
Bacterial cells of Staphylococcus aureus , one of the causal agents of mastitis in dairy cows. Its large capsule protects the organism from attack by the cow's immunological defenses.

Bacteria that are known to cause mastitis include:

These bacteria can be classified as environmental or contagious depending on mode and source of transmission.

Types of mastitis

Mastitis may be classified according two different criteria: either according to the clinical symptoms or depending on the mode of transmission.

Clinical symptoms
Mode of transmission

Transmission

Mastitis is most often transmitted by repetitive contact with the milking machine, and through contaminated hands or materials.

Another route is via the oral-to-udder transmission among calves. Feeding calves on milk may introduce some mastitis causing bacteria strain in the oral cavity of the calf where it will stay dormant until it is transmitted elsewhere. Since grouped calves like to stimulate suckling, they will transmit the bacteria to the udder tissue of their fellow calves. The bacteria will lay dormant in the udder tissue as the calf grows until it begins to lactate. That is when the bacteria activates and causes mastitis. This calls for strict calf management practices to curb this route of transmission. Micro-organisms enter through the teat tip into the teat duct where they get colonized due to the presence of leftover milk in the duct and subsequently spread throughout the udder causing infection. The effect of unhygienic milking machines and incomplete milking can help in this infection. [8]

Effects on milk composition

Serous exudate from udder in E. coli mastitis in cow (left), in comparison to normal milk (right) Mamite a colibacile laecea.jpg
Serous exudate from udder in E. coli mastitis in cow (left), in comparison to normal milk (right)

Mastitis can cause a decline in potassium and an increase in lactoferrin. It also results in decreased casein, the major protein in milk. As most calcium in milk is associated with casein, the disruption of casein synthesis contributes to lowered calcium in milk. The milk protein continues to undergo further deterioration during processing and storage. [9] Milk from cows with mastitis also has a higher somatic cell count. [10] Generally speaking, the higher the somatic cell count, the lower the milk quality. It also has a high microbial count. This reduces its yield. [11]

Constituents with increased constitution

The levels of total whey proteins (Mainly bovine serum albumin, immunoglobulins) (Na and Cl ions) and other ions (Cu, Fe, Zn) various enzymes and certain glycoproteins increase significantly. The pH of the milk increases too. Enzymes namely protease, lipase, aldolase, sorbitol, dehydrogenase also get increased.

Constituents with decreased constitution

The levels of lactose, fat, total casein (alpha and beta fractions decrease but gamma fraction increase ) some whey proteins (alpha-lactalbumin and beta-lactoglobulin) potassium and other minerals (Ca, Mg, P) decreases. Mastitic milk generally has lower SNF (solid-not-fat). Xanthine oxidase reduces by nearly half. [12]

Detection

A plastic paddle used in the California mastitis test. California Mastitis Test Schalmtestplatte.jpg
A plastic paddle used in the California mastitis test.

Cattle affected by mastitis can be detected by examining the udder for inflammation and swelling, or by observing the consistency of the milk, which will often develop clots or change color when a cow is infected. [13]

Another method of detection is the California mastitis test, which is designed to measure the milk's somatic cell count as a means for detecting inflammation and infection of the udder. [14]

The pH value of mastitic milk is higher than that of normal milk. On mixing 5ml of milk with 1 ml of bromothymol blue, the appearance of blue green colour indicated mastitic milk which has a pH of 6.8 or more as against the grass green colour produced by normal milk that has a pH of 6.6.

Normal milk has a chloride content of 0.08 to 0.14% whereas abnormal milk has more than 0.14%. The chloride content of milk can be estimated by addition of silver nitrate solution and 2 to 3 drops of potassium chromate as an indicator, A yellow colour indicates that the milk is abnormal.

Catalase test is also used to detect catalase which is present only in mastitic milk. [15]

Resazurin rennet test is based on the disturbance in the salt balance and increase in leucocyte content in mastitic milk. Coagulation of milk by rennet is sowed down due to disturbed salt balance and leucocytes reduce resazurin dye faster. Mastitic samples give delayed coagulation but faster resazurin reduction compared to normal milk [16]

Treatment

Treatment is possible with antibiotics – such as penicillin, but milk from such cows is not marketable until drug residues have left the cow's system. Antibiotics may be systemic (injected into the body), or they may be forced upwards into the teat through the teat canal (intramammary infusion). Cows being treated may be marked with tape to alert dairy workers, and their milk is syphoned off and discarded. To determine whether the levels of antibiotic residuals are within regulatory requirements, special tests exist. Vaccinations for mastitis are available, but as they only reduce the severity of the condition, and cannot prevent reoccurring infections, they should be used in conjunction with a mastitis prevention program.

Control

Practices such as good nutrition, proper milking hygiene, and the culling of chronically infected cows can help. Ensuring that cows have clean, dry bedding decreases the risk of infection and transmission. Dairy workers should wear rubber gloves while milking, and machines should be cleaned regularly to decrease the incidence of transmission.

Prevention

A good milking routine is vital. This usually consists of applying a pre-milking teat dip or spray, such as an iodine spray, and wiping teats dry prior to milking. The milking machine is then applied. After milking, the teats can be cleaned again to remove any growth medium for bacteria. A post milking product such as iodine-propylene glycol dip is used as a disinfectant and a barrier between the open teat and the bacteria in the air. Mastitis can occur after milking because the teat holes close after 15 minutes if the animal sits in a dirty place with feces and urine.

Industry costs

This disease costs the US dairy industry about 1.7 to 2 billion USD each year. [9]

Related Research Articles

<span class="mw-page-title-main">Dairy</span> Place where milk is stored and where butter and cheese are made or sold

A dairy is a place where milk is stored and where butter, cheese and other dairy products are made, or a place where those products are sold. It may be a room, a building or a larger establishment. In the United States, the word may also describe a dairy farm or the part of a mixed farm dedicated to milk for human consumption, whether from cows, buffaloes, goats, sheep, horses or camels.

<span class="mw-page-title-main">Mastitis</span> Medical condition

Mastitis is inflammation of the breast or udder, usually associated with breastfeeding. Symptoms typically include local pain and redness. There is often an associated fever and general soreness. Onset is typically fairly rapid and usually occurs within the first few months of delivery. Complications can include abscess formation.

<span class="mw-page-title-main">Dairy farming</span> Long-term production of milk

Dairy farming is a class of agriculture for the long-term production of milk, which is processed for the eventual sale of a dairy product. Dairy farming has a history that goes back to the early Neolithic era, around the seventh millennium BC, in many regions of Europe and Africa. Before the 20th century, milking was done by hand on small farms. Beginning in the early 20th century, milking was done in large scale dairy farms with innovations including rotary parlors, the milking pipeline, and automatic milking systems that were commercially developed in the early 1990s.

<span class="mw-page-title-main">Colostrum</span> Form of milk produced immediately following the delivery of newborn

Colostrum, or first milk, is the first form of milk produced by the mammary glands of humans and other mammals immediately following delivery of the newborn. It may be called beestings when referring to the first milk of a cow or similar animal. Most species will begin to generate colostrum just prior to giving birth. Colostrum has an especially high amount of bioactive compounds compared to mature milk to give the newborn the best possible start to life. Specifically, colostrum contains antibodies to protect the newborn against disease and infection, and immune and growth factors and other bioactives that help to activate a newborn's immune system, jumpstart gut function, and seed a healthy gut microbiome in the first few days of life. The bioactives found in colostrum are essential for a newborn's health, growth and vitality. Colostrum strengthens a baby's immune system and is filled with white blood cells to protect it from infection.

<span class="mw-page-title-main">Bovine somatotropin</span> Peptide hormone produced by cows pituitary glands

Bovine somatotropin or bovine somatotrophin, or bovine growth hormone (BGH), is a peptide hormone produced by cows' pituitary glands. Like other hormones, it is produced in small quantities and is used in regulating metabolic processes. Scientists created a bacterium that produces the hormone somatotropin which is produced by the cow's body after giving birth and increases milk production by around 10 percent.

<span class="mw-page-title-main">Udder</span> Organ formed of mammary glands on the females of dairy animals

An udder is an organ formed of two or four mammary glands on the females of dairy animals and ruminants such as cattle, goats, and sheep. An udder is equivalent to the breast in primates and elephantine pachyderms. The udder is a single mass hanging beneath the animal, consisting of pairs of mammary glands with protruding teats. In cattle, camels and deer, there are normally two pairs, in sheep and goats, there is one pair, and in some animals, there are many pairs. In animals with udders, the mammary glands develop on the milk line near the groin, and mammary glands that develop on the chest are generally referred to as breasts.

<span class="mw-page-title-main">Dairy cattle</span> Cattle bred to produce milk

Dairy cattle are cattle bred for the ability to produce large quantities of milk, from which dairy products are made. Dairy cattle generally are of the species Bos taurus.

<span class="mw-page-title-main">Milking</span> Removal of milk from the mammary glands of an animal

Milking is the act of removing milk from the mammary glands of cattle, water buffalo, humans, goats, sheep, and, more rarely, camels, horses, and donkeys. Milking may be done by hand or by machine, and requires the animal to be currently or recently pregnant. The milker may refer either to the animal that produces the milk or the person who milks said animal.

<span class="mw-page-title-main">Somatic cell count</span> Count of cells, usually to detect mastitis and thus to assess milk quality

A somatic cell count (SCC) is a cell count of somatic cells in a fluid specimen, usually milk. In dairying, the SCC is an indicator of the quality of milk—specifically, its low likeliness to contain harmful bacteria, and thus its high food safety. White blood cells (leukocytes) constitute the majority of somatic cells in question. The number of somatic cells increases in response to pathogenic bacteria like Staphylococcus aureus, a cause of mastitis. The SCC is quantified as cells per milliliter. General agreement rests on a reference range of less than 100,000 cells/mL for uninfected cows and greater than 250,000 for cows infected with significant pathogen levels. Several tests like the PortaSCC milk test and The California mastitis test provide a cow-side measure of somatic cell count. The somatic cell count in the milk also increases after calving when colostrum is produced.

<i>Neospora</i> Genus of single-celled organisms

Neospora is a single celled parasite of livestock and companion animals. It was not discovered until 1984 in Norway, where it was found in dogs. Neosporosis, the disease that affects cattle and companion animals, has a worldwide distribution. Neosporosis causes abortions in cattle and paralysis in companion animals. It is highly transmissible and some herds can have up to a 90% prevalence. Up to 33% of pregnancies can result in aborted fetuses on one dairy farm. In many countries this organism is the main cause of abortion in cattle. Neosporosis is now considered as a major cause of abortion in cattle worldwide. Many reliable diagnostic tests are commercially available. Neospora caninum does not appear to be infectious to humans. In dogs, Neospora caninum can cause neurological signs, especially in congenitally infected puppies, where it can form cysts in the central nervous system.

<span class="mw-page-title-main">Bovine papillomavirus</span> Group of viruses

Bovine papillomaviruses (BPV) are a paraphyletic group of DNA viruses of the subfamily Firstpapillomavirinae of Papillomaviridae that are common in cattle. All BPVs have a circular double-stranded DNA genome. Infection causes warts of the skin and alimentary tract, and more rarely cancers of the alimentary tract and urinary bladder. They are also thought to cause the skin tumour equine sarcoid in horses and donkeys.

<span class="mw-page-title-main">Cefquinome</span> Chemical compound

Cefquinome is a fourth-generation cephalosporin with pharmacological and antibacterial properties valuable in the treatment of coliform mastitis and other infections. It is only used in veterinary applications.

<span class="mw-page-title-main">United States raw milk debate</span>

The United States raw milk debate concerns issues of food safety and claimed health benefits of raw milk, and whether authorities responsible for regulating food safety should prohibit sale of raw milk for consumption.

<i>Streptococcus canis</i> Species of bacterium

Streptococcus canis is a group G beta-hemolytic species of Streptococcus. It was first isolated in dogs, giving the bacterium its name. These bacteria are characteristically different from Streptococcus dysgalactiae, which is a human-specific group G species that has a different phenotypic chemical composition. S. canis is important to the skin and mucosal health of cats and dogs, but under certain circumstances, these bacteria can cause opportunistic infections. These infections were known to afflict dogs and cats prior to the formal description of the species in Devriese et al., 1986. However, additional studies revealed cases of infection in other mammal species, including cattle and even humans. Instances of mortality from S. canis in humans are very low with only a few reported cases, while actual instances of infection may be underreported due to mischaracterizations of the bacteria as S. dysgalactiae. This species, in general, is highly susceptible to antibiotics, and plans to develop a vaccine to prevent human infections are currently being considered.

<span class="mw-page-title-main">Cattle</span> Large, domesticated, cloven-hooved herbivores

Cattle are large, domesticated, bovid ungulates widely kept as livestock. They are prominent modern members of the subfamily Bovinae and the most widespread species of the genus Bos. Mature female cattle are called cows and mature male cattle are bulls. Young female cattle are called heifers, young male cattle are oxen or bullocks, and castrated male cattle are known as steers.

<span class="mw-page-title-main">Metritis</span> Inflammation of the wall of the uterus

Metritis is inflammation of the wall of the uterus, whereas endometritis is inflammation of the functional lining of the uterus, called the endometrium. The term pelvic inflammatory disease (PID) is often used for metritis.

<span class="mw-page-title-main">Enzybiotics</span> Experimental antibacterial therapy

Enzybiotics are an experimental antibacterial therapy. The term is derived from a combination of the words “enzyme” and “antibiotics.” Enzymes have been extensively utilized for their antibacterial and antimicrobial properties. Proteolytic enzymes called endolysins have demonstrated particular effectiveness in combating a range of bacteria and are the basis for enzybiotic research. Endolysins are derived from bacteriophages and are highly efficient at lysing bacterial cells. Enzybiotics are being researched largely to address the issue of antibiotic resistance, which has allowed for the proliferation of drug-resistant pathogens posing great risk to animal and human health across the globe.

Mycoplasma bovis is one of 126 species of genus Mycoplasma. It is the smallest living cell and anaerobic organism in nature. It does not contain any cell wall and is therefore resistant to penicillin and other beta lactam antibiotics.

<span class="mw-page-title-main">Dry cow</span>

A dry cow refers to a dairy cow that is in a stage of their lactation cycle where milk production ceases prior to calving. This part of their lactation cycle is referred to as the cows dry period and typically last between 40 and 65 days. Dry cows are typically divided into two groups: far-off and close-up. Once the cow has entered this stage, producers will seal the cows teat while following a veterinarian recommended, dry cow therapy for their herd. This dry period is a critical part of their lactation cycle and is important for the cows health, the newborn calf and future milk production, as it allows the cow time to rest, eat and prepare for birth. During this time, the cow will produce colostrum for the newly born calf.

<span class="mw-page-title-main">Milk borne diseases</span>

Milk borne diseases are any diseases caused by consumption of milk or dairy products infected or contaminated by pathogens. Milk borne diseases are one of the recurrent foodborne illnesses—between 1993 and 2012 over 120 outbreaks related to raw milk were recorded in the US with approximately 1,900 illnesses and 140 hospitalisations. With rich nutrients essential for growth and development such as proteins, lipids, carbohydrates, and vitamins in milk, pathogenic microorganisms are well nourished and are capable of rapid cell division and extensive population growth in this favourable environment. Common pathogens include bacteria, viruses, fungi, and parasites and among them, bacterial infection is the leading cause of milk borne diseases.

References

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Further reading