Mitochondrial antiviral-signaling protein

Last updated
MAVS
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases MAVS , CARDIF, IPS-1, IPS1, VISA, mitochondrial antiviral signaling protein
External IDs OMIM: 609676 MGI: 2444773 HomoloGene: 17004 GeneCards: MAVS
Orthologs
SpeciesHumanMouse
Entrez

57506

228607

Ensembl

ENSG00000088888

ENSMUSG00000037523

UniProt

Q7Z434

Q8VCF0

RefSeq (mRNA)

NM_001206491
NM_020746
NM_001385663

NM_001206382
NM_001206383
NM_001206385
NM_144888

RefSeq (protein)

NP_001193420
NP_065797

NP_001193311
NP_001193312
NP_001193314
NP_659137

Location (UCSC) Chr 20: 3.85 – 3.88 Mb Chr 2: 131.08 – 131.09 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Mitochondrial antiviral-signaling protein (MAVS) is a protein that is essential for antiviral innate immunity. MAVS is located in the outer membrane of the mitochondria, peroxisomes, and mitochondrial-associated endoplasmic reticulum membrane (MAM). [5] [6] Upon viral infection, a group of cytosolic proteins will detect the presence of the virus and bind to MAVS, thereby activating MAVS. The activation of MAVS leads the virally infected cell to secrete cytokines. This induces an immune response which kills the host's virally infected cells, resulting in clearance of the virus.

Contents

Structure

Schematic representation of MAVS gene. Ribosome recognition sites for translation are located at position 1 and position 142 on the MAVS gene, highlighted by the yellow band. Schematic representation of MAVS gene.png
Schematic representation of MAVS gene. Ribosome recognition sites for translation are located at position 1 and position 142 on the MAVS gene, highlighted by the yellow band.

MAVS is also known as IFN-β promoter stimulator I (IPS-1), caspase activation recruitment domain adaptor inducing IFN-β(CARDIF), or virus induced signaling adaptor (VISA). [7] MAVS is encoded by a MAVS gene. [7] [8] MAVS is a 540 amino acid protein that consists of three components, a N terminal caspase activation recruitment domain (CARD), a proline rich domain, and a transmembrane C terminal domain (TM). [7]

After the MAVS gene has been transcribed into RNA, ribosomes can translate the MAVS protein from two different sites. [7] The initial translation site generates the full-length MAVS protein. The alternative translation site generates a shorter protein, termed as “miniMAVS” or short-MAVS (sMAVS). [7] sMAVS is a 398 amino acid MAVS protein that lacks the CARD domain. This is significant because the CARD domain is where two cytosolic proteins bind to activate MAVS, signaling that there is a virus present in the cell. [7]

Function

Double stranded RNA viruses are recognized by either the transmembrane toll-like receptor 3 (TLR3) or by one of two cytosolic proteins, retinoic acid-inducible gene I (RIG-I)-like receptors and melanoma differentiation-associated gene 5 (MDA5). [7] [8] [9] [10] RIG-I and MDA5 differ in the viral RNA that they recognize, but they share many structural features, including the N-terminal CARD that allows them to bind to MAVS. [7] MAVS activation leads to the increased levels of pro-inflammatory cytokines via activation of transcription factors, nuclear factor kB (NF-kB), interferon regulatory factor 1 (IRF1), and interferon regulatory factor 3 (IRF3). [7] [8] [9] NFkB, IRF1, and IRF3 are transcription factors and play critical roles in the production of cytokines.

Cellular mechanisms of MAVS pathway Cellular mechanisms of MAVS pathway.pdf
Cellular mechanisms of MAVS pathway

At a resting state for the cell, a protein called mitofusin 2 (MFN2) is known to interact with MAVS, preventing MAVS from binding to the cytosolic proteins, such as RIG-I and MDA5. [7] [8] Upon recognition of the virus in the cytosol, mitochondria-associated ER membranes (MAM) and mitochondria will become physically tethered by MFN2 and RIG-I binds to a second RIG-I protein to form a protein complex. [7] [8] [9] This complex binds to TRIM25 and molecular chaperone 14-3-3e to form a complex termed “translocon”. [7] [8] [9] [10] The translocon travels to the mitochondria where it binds to the CARD region on MAVS, leading to activation of MAVS. [7] [8] [9] [10] Subsequently, MAVS proteins bind to each other through the CARD and TM domain to recruit several downstream signaling factors to form the MAVS signaling complex. [7] [8] The formation of this MAVS signaling complex is aided by augmented levels of mitochondrial reactive oxygen species (mROS), independent of the RNA sensing. [8] [9] The MAVS signaling complex interacts with TANK binding kinase 1 and/or protein kinases IKKA (CHUK) and IKKB (IKBKB), which leads to the phosphorylation and nuclear translocation of IRF3. [7] Although MAVS signal transduction and regulation is not fully understood, activated MAVS proteins in the mitochondria, ER, and peroxisome are needed to maximize the antiviral innate immune response.

MAVS protein induces apoptosis in host virally infected cells by interacting with a protease called caspase 8. [7] Activation of apoptosis by caspase 8 is independent of the Bax/Bak apoptotic pathway, the main pathway of apoptosis in cells. [7]

Viral evasion

Certain viruses, such as human cytomegalovirus (HCMV) and hepatitis C (HCV), have adapted to suppress the function of MAVS in the antiviral innate immune response, aiding in viral replication. [7] [11] HCMV impairs MAVS through the viral mitochondria-localized inhibitor of apoptosis protein (vMIA), thus reducing the pro-inflammatory cytokine response. [11] vMIA also localizes to the peroxisome where it interacts with cytoplasmic chaperone protein Pex19, disabling the transport machinery of peroxisomal membrane proteins. [11] The HCV NS3-NS4A strain inactivates MAVS signaling by cleaving the MAVS protein directly upstream of MAVS membrane-targeting domain in the MAM and peroxisome, preventing MAVS downstream signaling. [7]

Regulation

The expression and function of MAVS are regulated at the transcriptional, posttranscriptional, and posttranslational level. At the transcriptional level, the reactive oxygen species (ROS) generated during antiviral response acts as a negative regulator. [7] [8] [9] MAVS, additionally, encodes a number of splice variants that have been proposed to regulate MAVS. At the post-transcriptional level, there are two translational sites present on MAVS that can generate two proteins of MAVS. The alternative translation site resides upstream, resulting in expression of sMAVS. [7] [8] [9] At the translational level, proteins such as a family of ubiquitin E3 ligase regulate MAVS activity. [7] [8] [9]

Related Research Articles

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Pattern recognition receptors (PRRs) play a crucial role in the proper function of the innate immune system. PRRs are germline-encoded host sensors, which detect molecules typical for the pathogens. They are proteins expressed mainly by cells of the innate immune system, such as dendritic cells, macrophages, monocytes, neutrophils, as well as by epithelial cells, to identify two classes of molecules: pathogen-associated molecular patterns (PAMPs), which are associated with microbial pathogens, and damage-associated molecular patterns (DAMPs), which are associated with components of host's cells that are released during cell damage or death. They are also called primitive pattern recognition receptors because they evolved before other parts of the immune system, particularly before adaptive immunity. PRRs also mediate the initiation of antigen-specific adaptive immune response and release of inflammatory cytokines.

<span class="mw-page-title-main">Innate immune system</span> Immunity strategy in living beings

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<span class="mw-page-title-main">CARD (domain)</span> Interaction motifs found in a wide array of proteins

Caspase recruitment domains, or caspase activation and recruitment domains (CARDs), are interaction motifs found in a wide array of proteins, typically those involved in processes relating to inflammation and apoptosis. These domains mediate the formation of larger protein complexes via direct interactions between individual CARDs. CARDs are found on a strikingly wide range of proteins, including helicases, kinases, mitochondrial proteins, caspases, and other cytoplasmic factors.

<span class="mw-page-title-main">IRF3</span> Protein-coding gene in the species Homo sapiens

Interferon regulatory factor 3, also known as IRF3, is an interferon regulatory factor.

<span class="mw-page-title-main">Toll-like receptor 6</span> Protein found in humans

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<span class="mw-page-title-main">RIG-I</span> Mammalian protein found in humans

RIG-I is a cytosolic pattern recognition receptor (PRR) that can mediate induction of a type-I interferon (IFN1) response. RIG-I is an essential molecule in the innate immune system for recognizing cells that have been infected with a virus. These viruses can include West Nile virus, Japanese Encephalitis virus, influenza A, Sendai virus, flavivirus, and coronaviruses.

<span class="mw-page-title-main">TANK-binding kinase 1</span> Protein-coding gene in the species Homo sapiens

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<span class="mw-page-title-main">IRF5</span> Protein-coding gene in the species Homo sapiens

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<span class="mw-page-title-main">MDA5</span> Mammalian protein found in Homo sapiens

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<span class="mw-page-title-main">MUL1</span> Protein-coding gene in the species Homo sapiens

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<span class="mw-page-title-main">ECSIT</span> Protein-coding gene in the species Homo sapiens

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<span class="mw-page-title-main">SNX8</span> Protein

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References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000088888 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000037523 - Ensembl, May 2017
  3. ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ↑ Vazquez C, Beachboard DC, Horner SM (2017). "Methods to Visualize MAVS Subcellular Localization". Innate Antiviral Immunity. Methods in Molecular Biology. Vol. 1656. pp. 131–142. doi:10.1007/978-1-4939-7237-1_7. ISBN   978-1-4939-7236-4. PMC   6103534 . PMID   28808966.
  6. ↑ Seth RB, Sun L, Ea CK, Chen ZJ (September 2005). "Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3". Cell. 122 (5): 669–682. doi: 10.1016/j.cell.2005.08.012 . PMID   16125763. S2CID   11104354.
  7. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 Vazquez C, Horner SM (July 2015). "MAVS Coordination of Antiviral Innate Immunity". Journal of Virology. 89 (14): 6974–6977. doi:10.1128/JVI.01918-14. PMC   4473567 . PMID   25948741.
  8. 1 2 3 4 5 6 7 8 9 10 11 12 Mohanty A, Tiwari-Pandey R, Pandey NR (September 2019). "Mitochondria: the indispensable players in innate immunity and guardians of the inflammatory response". Journal of Cell Communication and Signaling. 13 (3): 303–318. doi:10.1007/s12079-019-00507-9. PMC   6732146 . PMID   30719617.
  9. 1 2 3 4 5 6 7 8 9 Jacobs JL, Coyne CB (December 2013). "Mechanisms of MAVS regulation at the mitochondrial membrane". Journal of Molecular Biology. 425 (24): 5009–5019. doi:10.1016/j.jmb.2013.10.007. PMC   4562275 . PMID   24120683.
  10. 1 2 3 Jiang QX (2019). "Structural Variability in the RLR-MAVS Pathway and Sensitive Detection of Viral RNAs". Medicinal Chemistry. 15 (5): 443–458. doi:10.2174/1573406415666181219101613. PMC   6858087 . PMID   30569868.
  11. 1 2 3 Ashley CL, Abendroth A, McSharry BP, Slobedman B (2019). "Interferon-Independent Innate Responses to Cytomegalovirus". Frontiers in Immunology. 10: 2751. doi: 10.3389/fimmu.2019.02751 . PMC   6917592 . PMID   31921100.

Further reading

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