NOL1

NOP2
Identifiers
Aliases	NOP2 , NOL1, NOP120, NSUN1, p120, NOP2 nucleolar protein
External IDs	OMIM: 164031; MGI: 107891; HomoloGene: 135865; GeneCards: NOP2; OMA:NOP2 - orthologs
Gene location (Human) Chr. Chromosome 12 (human) [1] Band 12p13.31 Start 6,556,863 bp [1] End 6,568,691 bp [1]
Gene location (Mouse) Chr. Chromosome 6 (mouse) [2] Band 6 F2|6 59.32 cM Start 125,108,872 bp [2] End 125,121,716 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in granulocyte lymph node appendix spleen left uterine tube gastrocnemius muscle islet of Langerhans right lobe of thyroid gland anterior pituitary tonsil Top expressed in epiblast otic placode otic vesicle morula morula superior surface of tongue gallbladder somite embryo embryo More reference expression data BioGPS More reference expression data
Gene ontology Molecular function methyltransferase activity transferase activity protein binding S-adenosylmethionine-dependent methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity RNA binding Cellular component nucleus nucleolus nucleoplasm Biological process rRNA processing methylation rRNA base methylation maturation of LSU-rRNA positive regulation of cell population proliferation regulation of transcription by RNA polymerase II ribosomal large subunit assembly regulation of signal transduction by p53 class mediator ribosome biogenesis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 4839 110109 Ensembl ENSG00000111641 ENSMUSG00000038279 UniProt P46087 Q922K7 RefSeq (mRNA) NM_001033714 NM_001258308 NM_001258309 NM_001258310 NM_006170 NM_138747 RefSeq (protein) NP_001028886 NP_001245237 NP_001245238 NP_001245239 NP_006161 n/a Location (UCSC) Chr 12: 6.56 – 6.57 Mb Chr 6: 125.11 – 125.12 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Putative ribosomal RNA methyltransferase NOP2 is an enzyme that in humans is encoded by the NOP2 gene. ^{[5] [6]}

The protein encoded by this gene is a nucleolar antigen expressed in proliferating cells. It is not detectable in non-proliferating normal tissue but is detectable in many human tumors. ^[7]

Overexpression of p120 leads to malignant transformation of 3T3 cells while treatment with antisense p120 mRNA causes the transformed cells to revert to their original non-malignant phenotype. ^[8] The p120 protein displays a dramatic increase in expression at the G1/S transition suggesting that p120 regulates the cell cycle and nucleolar activity that is required for cell proliferation. ^[9]

Interactions

NOL1 has been shown to interact with MCRS1. ^[10]

References

1. GRCh38: Ensembl release 89: ENSG00000111641 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000038279 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Baens M, Chaffanet M, Aerssens J, Cassiman JJ, Marynen P (October 1994). "Assignment of the gene for the human proliferating cell nucleolar protein P120 (NOL1) to chromosome 12p13 by fluorescence in situ hybridization and polymerase chain reaction with somatic cell hybrids". Genomics. 21 (1): 296–7. doi:10.1006/geno.1994.1267. PMID   8088812.
6. "Entrez Gene: NOL1 nucleolar protein 1, 120kDa".
7. Freeman JW, Busch RK, Gyorkey F, Gyorkey P, Ross BE, Busch H (1 March 1988). "Identification and characterization of a human proliferation-associated nucleolar antigen with a molecular weight of 120,000 expressed in early G1 phase". Cancer Res. 48 (5): 1244–51. PMID   3422591.
8. Perlaky L, Valdez BC, Busch RK, Larson RG, Jhiang SM, Zhang WW, Brattain M, Busch H (January 1992). "Increased growth of NIH/3T3 cells by transfection with human p120 complementary DNA and inhibition by a p120 antisense construct". Cancer Res. 52 (2): 428–36. PMID   1728415.
9. Fonagy A, Swiderski C, Wilson A, Bolton W, Kenyon N, Freeman JW (January 1993). "Cell cycle regulated expression of nucleolar antigen P120 in normal and transformed human fibroblasts". J. Cell. Physiol. 154 (1): 16–27. doi:10.1002/jcp.1041540104. PMID   8419402. S2CID   45238441.
10. Ren Y, Busch RK, Perlaky L, Busch H (May 1998). "The 58-kDa microspherule protein (MSP58), a nucleolar protein, interacts with nucleolar protein p120". Eur. J. Biochem. 253 (3): 734–42. doi: 10.1046/j.1432-1327.1998.2530734.x . PMID   9654073.

Further reading

• Busch H (1990). "The final common pathway of cancer". Cancer Res. 50 (16): 4830–8. PMID   2199030.
• Valdez BC, Perlaky L, Saijo Y, Henning D, Zhu C, Busch RK, Zhang WW, Busch H (1992). "A region of antisense RNA from human p120 cDNA with high homology to mouse p120 cDNA inhibits NIH 3T3 proliferation". Cancer Res. 52 (20): 5681–6. PMID   1394192.
• Larson RG, Henning D, Haidar MA, Jhiang S, Lin WL, Zhang WW, Busch H (1990). "Genomic structure of the human proliferating cell nucleolar protein P120". Cancer Commun. 2 (2): 63–71. doi: 10.3727/095535490820874704 . PMID   2372471.
• Fonagy A, Henning D, Jhiang S, Haidar M, Busch RK, Larson R, Valdez B, Busch H (1989). "Cloning of the cDNA and sequence of the human proliferating-cell nucleolar protein P120". Cancer Commun. 1 (4): 243–51. PMID   2576976.
• Freeman JW, Busch RK, Gyorkey F, Gyorkey P, Ross BE, Busch H (1988). "Identification and characterization of a human proliferation-associated nucleolar antigen with a molecular weight of 120,000 expressed in early G1 phase". Cancer Res. 48 (5): 1244–51. PMID   3422591.
• Valdez BC, Perlaky L, Henning D, Saijo Y, Chan PK, Busch H (1994). "Identification of the nuclear and nucleolar localization signals of the protein p120. Interaction with translocation protein B23". J. Biol. Chem. 269 (38): 23776–83. doi: 10.1016/S0021-9258(17)31583-1 . PMID   8089149.
• Jallal B, Mossie K, Vasiloudis G, Knyazev P, Zachwieja J, Clairvoyant F, Schilling J, Ullrich A (1997). "The receptor-like protein-tyrosine phosphatase DEP-1 is constitutively associated with a 64-kDa protein serine/threonine kinase". J. Biol. Chem. 272 (18): 12158–63. doi: 10.1074/jbc.272.18.12158 . PMID   9115287.
• Ren Y, Busch RK, Perlaky L, Busch H (1998). "The 58-kDa microspherule protein (MSP58), a nucleolar protein, interacts with nucleolar protein p120". Eur. J. Biochem. 253 (3): 734–42. doi: 10.1046/j.1432-1327.1998.2530734.x . PMID   9654073.
• Colombo E, Marine JC, Danovi D, Falini B, Pelicci PG (2002). "Nucleophosmin regulates the stability and transcriptional activity of p53". Nat. Cell Biol. 4 (7): 529–33. doi:10.1038/ncb814. PMID   12080348. S2CID   24923289.
• Holsinger LJ, Ward K, Duffield B, Zachwieja J, Jallal B (2002). "The transmembrane receptor protein tyrosine phosphatase DEP1 interacts with p120(ctn)". Oncogene. 21 (46): 7067–76. doi:10.1038/sj.onc.1205858. PMID   12370829. S2CID   25745858.
• Scherl A, Couté Y, Déon C, Callé A, Kindbeiter K, Sanchez JC, Greco A, Hochstrasser D, Diaz JJ (2002). "Functional proteomic analysis of human nucleolus". Mol. Biol. Cell. 13 (11): 4100–9. doi:10.1091/mbc.E02-05-0271. PMC   133617 . PMID   12429849.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi: 10.1073/pnas.0404720101 . PMC   514446 . PMID   15302935.
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC   1356129 . PMID   16344560.
• Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. Bibcode:2006PNAS..103.5391N. doi: 10.1073/pnas.0507066103 . PMC   1459365 . PMID   16565220.
• Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID   16964243. S2CID   14294292.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi: 10.1016/j.cell.2006.09.026 . PMID   17081983. S2CID   7827573.