NOL12

NOL12
Identifiers
Aliases	NOL12 , Nop25, dJ37E16.7, nucleolar protein 12
External IDs	MGI: 2146285 HomoloGene: 32577 GeneCards: NOL12
Gene location (Human) Chr. Chromosome 22 (human) [1] Band 22q13.1 Start 37,681,673 bp [1] End 37,693,476 bp [1]
Gene location (Mouse) Chr. Chromosome 15 (mouse) [2] Band 15|15 E1 Start 78,819,133 bp [2] End 78,827,838 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sural nerve monocyte right adrenal gland right lobe of thyroid gland left lobe of thyroid gland skin of abdomen minor salivary gland body of stomach prefrontal cortex rectum Top expressed in interventricular septum morula internal carotid artery fossa condyle external carotid artery vas deferens blastocyst medial ganglionic eminence yolk sac More reference expression data BioGPS More reference expression data
Gene ontology Molecular function rRNA binding protein binding RNA binding single-stranded DNA binding Cellular component nucleolus nucleus Biological process rRNA processing nucleolus organization positive regulation of cell population proliferation positive regulation of cell growth negative regulation of apoptotic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 79159 97961 Ensembl ENSG00000273899 ENSMUSG00000033099 UniProt Q9UGY1 Q8BG17 RefSeq (mRNA) NM_024313 NM_133800 RefSeq (protein) NP_077289 NP_598561 Location (UCSC) Chr 22: 37.68 – 37.69 Mb Chr 15: 78.82 – 78.83 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Nucleolar protein 12 is a protein that in humans is encoded by the NOL12 gene. ^{[5] [6]} Human NOL12 has been shown to localize in the nucleolus and regulate nucleolar structure and homeostasis by maintaining the levels of multi-functional fibrillarin and nucleolin proteins. Its deficiency leads to p53 activation resulting in G2 arrest of cell. Nol12 or hNol12 drives p53 induced cell senescence suggesting its important role in aging. ^[7] Human Nol12 is also required for ribosome maturation and genome integrity. ^[8]

Nol12 Family

Human Nol12 belongs to family of proteins known as ribosomal binding proteins RBPs. The human members have equivalents in different species. Its homologue in Drosophila melanogaster is called Viriato which is involved in eye and nervous system development and without Viriato, Drosophila melanogaster eye development fails. Loss of also Viriato resulted in cell proliferation, developmental delays and apoptosis. ^{[9] [10]} hNOL12 homologue in rats is known as Nop25 and is responsible for nucleolar structure and integrity. ^[11] Its yeast homologue has been named as Rrp17 which has been shown to possess 5′-3′ exonuclease activity and is involved in ribosome biogenesis. ^[12]

References

1. GRCh38: Ensembl release 89: ENSG00000273899 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000033099 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, et al. (December 2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proceedings of the National Academy of Sciences of the United States of America. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
6. "Entrez Gene: NOL12 nucleolar protein 12".
7. Pinho M, Macedo JC, Logarinho E, Pereira PS (June 2019). "NOL12 Repression Induces Nucleolar Stress-Driven Cellular Senescence and Is Associated with Normative Aging". Molecular and Cellular Biology. 39 (12). doi:10.1128/MCB.00099-19. PMC   6549468 . PMID   30988155.
8. Scott DD, Trahan C, Zindy PJ, Aguilar LC, Delubac MY, Van Nostrand EL, et al. (December 2017). "Nol12 is a multifunctional RNA binding protein at the nexus of RNA and DNA metabolism". Nucleic Acids Research. 45 (21): 12509–12528. doi: 10.1093/nar/gkx963 . PMC   5716212 . PMID   29069457.
9. Marinho J, Martins T, Neto M, Casares F, Pereira PS (May 2013). "The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development". Developmental Biology. 377 (1): 154–65. doi: 10.1016/j.ydbio.2013.02.003 . hdl: 10316/26860 . PMID   23416177.
10. Marinho J, Casares F, Pereira PS (January 2011). "The Drosophila Nol12 homologue viriato is a dMyc target that regulates nucleolar architecture and is required for dMyc-stimulated cell growth". Development. 138 (2): 349–57. doi: 10.1242/dev.054411 . hdl: 10261/41278 . PMID   21177347. S2CID   18287789.
11. Suzuki S, Fujiwara T, Kanno M (July 2007). "Nucleolar protein Nop25 is involved in nucleolar architecture". Biochemical and Biophysical Research Communications. 358 (4): 1114–9. doi:10.1016/j.bbrc.2007.05.069. PMID   17524363.
12. Oeffinger M, Zenklusen D, Ferguson A, Wei KE, El Hage A, Tollervey D, et al. (December 2009). "Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNA". Molecular Cell. 36 (5): 768–81. doi:10.1016/j.molcel.2009.11.011. PMC   2806520 . PMID   20005841.

Further reading

• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (November 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi: 10.1016/j.cell.2006.09.026 . PMID   17081983. S2CID   7827573.
• Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R (April 2006). "Phosphoproteome analysis of the human mitotic spindle". Proceedings of the National Academy of Sciences of the United States of America. 103 (14): 5391–6. Bibcode:2006PNAS..103.5391N. doi: 10.1073/pnas.0507066103 . PMC   1459365 . PMID   16565220.
• Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, et al. (2005). "A genome annotation-driven approach to cloning the human ORFeome". Genome Biology. 5 (10): R84. doi: 10.1186/gb-2004-5-10-r84 . PMC   545604 . PMID   15461802.
• Collins JE, Goward ME, Cole CG, Smink LJ, Huckle EJ, Knowles S, et al. (January 2003). "Reevaluating human gene annotation: a second-generation analysis of chromosome 22". Genome Research. 13 (1): 27–36. doi:10.1101/gr.695703. PMC   430954 . PMID   12529303.
• Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, et al. (December 1999). "The DNA sequence of human chromosome 22". Nature. 402 (6761): 489–95. Bibcode:1999Natur.402..489D. doi: 10.1038/990031 . PMID   10591208.