NP-40

Last updated
NP-40
Polyethylene glycol nonylphenyl ether.svg
Names
Other names
Polyethylene glycol nonylphenyl ether; Nonyl phenoxypolyethoxylethanol; Nonoxynol-40
Identifiers
3D model (JSmol)
ChEMBL
EC Number
  • 500-024-6
PubChem CID
UNII
UN number 3082
  • InChI=1S/C19H32O3/c1-2-3-4-5-6-7-8-9-18-10-12-19(13-11-18)22-17-16-21-15-14-20/h10-13,20H,2-9,14-17H2,1H3
    Key: BLXVTZPGEOGTGG-UHFFFAOYSA-N
  • CCCCCCCCCC1=CC=C(C=C1)OCCOCCO
Properties
H(C2H4O)nO(C6H4)C9H19
Molar mass Variable
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

NP-40 (also known as Tergitol-type NP-40 and nonyl phenoxypolyethoxylethanol [1] ) is a commercially available detergent with CAS Registry Number 9016-45-9. NP-40 is an ethoxylated nonylphenol for non-ionic surfactants and can act as emulsifier and demulsifier agent.

NP-40 is often used to break open all membranes within a cell, including the nuclear membrane [ citation needed ]. To break only the cytoplasmic membrane, other detergents such as digitonin can be used.

NP-40 has applications in paper and textile processing, in paints and coatings, and in agrochemical manufacturing.

Care should be taken to avoid confusing NP-40 with Nonidet P-40 (octyl phenoxypolyethoxylethanol) which is currently out of production. Nonidet P-40 ("Non-Ionic Detergent") was originally manufactured and trademarked by the Shell Chemical Company, [2] but was phased out of production in the early 2000s. Confusingly, biochemical protocols published between the 1960s [3] and 2000s refer to Shell's Nonidet P-40 as NP-40. Shell's original Nonidet P-40 had a hydrophilic-lipophilic balance (HLB) value of 13.5, [4] as opposed to 12.9 for the currently available IGEPAL CA-630, [5] indicating that the currently available compound is more potent than the compound used in older publications. Indeed, according to a 2017 report, [6] an additional dilution factor of 10 was required for the currently available NP-40 ("Nonidet P-40 substitutes") to match the activity of the previously available, and now discontinued, Shell's Nonidet P-40.


See also

Related Research Articles

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<span class="mw-page-title-main">Quaternary ammonium cation</span> Polyatomic ions of the form N(–R)₄ (charge +1)

In chemistry, quaternary ammonium cations, also known as quats, are positively-charged polyatomic ions of the structure [NR4]+, where R is an alkyl group, an aryl group or organyl group. Unlike the ammonium ion and the primary, secondary, or tertiary ammonium cations, the quaternary ammonium cations are permanently charged, independent of the pH of their solution. Quaternary ammonium salts or quaternary ammonium compounds are salts of quaternary ammonium cations. Polyquats are a variety of engineered polymer forms which provide multiple quat molecules within a larger molecule.

<span class="mw-page-title-main">Nonylphenol</span> Chemical compound

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<span class="mw-page-title-main">Wetting solution</span>

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References

  1. NP-40 Surfact-Amps Detergent Solution
  2. "Front matter". Annual Reports on the Progress of Chemistry. 57: P001. 1960. doi:10.1039/AR96057FP001.
  3. "CAB Direct".
  4. TERGITOL NP-9 Surfactant
  5. "Guide to Protein Purification, Volume 463 - 2nd Edition".
  6. Sinha, S.; Field, J.J.; Miller, J.H. (2017). "Use of substitute Nonidet P-40 nonionic detergents in intracellular tubulin polymerization assays for screening of microtubule targeting agents". Biochemistry and Cell Biology. 95 (3): 379–384. doi: 10.1139/bcb-2016-0141 . PMID   28177759.