NUBP2

NUBP2
Identifiers
Aliases	NUBP2 , CFD1, NBP 2, NUBP1, nucleotide binding protein 2, CIAO6, NUBP iron-sulfur cluster assembly factor 2, cytosolic
External IDs	OMIM: 610779; MGI: 1347072; HomoloGene: 8057; GeneCards: NUBP2; OMA:NUBP2 - orthologs
Gene location (Human) Chr. Chromosome 16 (human) [1] Band 16p13.3 Start 1,782,932 bp [1] End 1,789,186 bp [1]
Gene location (Mouse) Chr. Chromosome 17 (mouse) [2] Band 17 A3.3|17 12.53 cM Start 24,882,611 bp [2] End 24,886,349 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in thymus right lobe of liver right hemisphere of cerebellum apex of heart muscle of thigh fundus body of pancreas right adrenal gland gastrocnemius muscle left adrenal cortex Top expressed in spermatocyte seminiferous tubule spermatid right kidney morula fetal liver hematopoietic progenitor cell yolk sac blastocyst embryo proximal tubule More reference expression data BioGPS n/a
Gene ontology Molecular function 4 iron, 4 sulfur cluster binding iron-sulfur cluster binding protein binding ATP binding metal ion binding nucleotide binding Cellular component cytoplasm centriole cell projection spindle pole centrosome cilium cytoskeleton nucleus microtubule organizing center cytosol Biological process cell projection organization iron-sulfur cluster assembly Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10101 26426 Ensembl ENSG00000095906 ENSMUSG00000039183 UniProt Q9Y5Y2 Q9R061 RefSeq (mRNA) NM_001284501 NM_001284502 NM_012225 NM_011956 NM_001355396 RefSeq (protein) NP_001271430 NP_001271431 NP_036357 NP_036086 NP_001342325 Location (UCSC) Chr 16: 1.78 – 1.79 Mb Chr 17: 24.88 – 24.89 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

ParA/MinD ATPase like
Identifiers
Symbol	ParA
Pfam	PF10609
InterPro	IPR019591
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Nucleotide-binding protein 2 (NBP 2) also known as cytosolic Fe-S cluster assembly factor NUBP2 is a protein that in humans is encoded by the NUBP2 gene. ^[5]

NUBP2 is a member of the NUBP/MRP gene subfamily of ATP-binding proteins. ^[6] There are two types in eukaryotes NUBP1 and NUBP2, and one novel human gene that define NBP nucleotide-binding proteins (NUBP/MRP-multidrug resistance-associated protein) ^[5] in mammalian cells requires the maturation of cytosolic ^[7] iron-sulfur (Fe/S) ^[8] proteins as Nubp1 is involved in the formation of extramitochondrial Fe/S proteins ^[6] the cell division inhibitor MinD is homologous ^[9] and involve two proteins components of the (FeS) protein assembly machinery closely similar cytosolic ^[6] soluble ^[8] P loop ^[9] NTPase where Nar1 ^{[10] [11]} is required for assembly, ^[12] identified Cfd1p ^{[13] [14]} in cytosolic and nuclear Fe/S protein biogenesis ^[8] in yeast. ^[15] Nubp proteins NTPase Nbp35p. ^{[11] [12]} MinD is homologous to members in MinD of E. coli, a relative of the ParA family. ^{[9] [16] [17]}

Morphology

^{Further information: Morphology (biology)}

NBP35 bacterial plasmids F (the classical Escherichia coli sex factor) ^[9] is found in all nuclear genes in vegetative and gametic flagella of the unicellular green algae C. reinhardtii and nuclear Fe/S protein biogenesis required for cytosolic iron-sulfur protein assembly; MNP =MRP-like; MRP (Multiple Resistance and pH adaptation) MRP/NBP35-like P-loop NTPase similar to; and functions as minD_arch; cell division ATPase MinD, archaeal and homologue's of NUBP1. The NBP35 gene is conserved in archaea ^[18] Bacteria, Metazoa, Fungi and other Eukaryotes and with considerable divergence from the yeast; Cfd1-Nbp35 Fe-S to man. In a scaffold complex ^[19] protein to form large molecular assemblies that store Fe(III) and 4Fe-4S seen as secondary to defects inactivated to accomplish its functions as physiologically relevant form(s) Fe/S proteins Iron regulatory protein 1 (IRP1) is regulated through ^[14] prevents deficiencies and increased mutation rates ^[17] that characterized a plant P loop NTPase with sequence similarity to Nbp35 homologue's of NUBP1. ^[20]

Interactions

NUBP2 has been shown to interact with...

• ACO1 Iron-responsive element-binding protein 1 (IRE-BP 1) (Iron regulatory protein 1) (IRP1) ^{[14] [21]}
• MAPK8IP3 C-jun-amino-terminal kinase-interacting protein 3 (JNK-interacting protein 3) (JIP-3) ^[22]
• IGFALS Insulin-like growth factor-binding protein complex acid labile chain precursor (ALS) ^{[22] [23]}
• KIF11 Kinesin-like protein KIF11 (Kinesin-related motor protein Eg5) ^[24]
• SEPP1 Selenoprotein P precursor (SeP) ^[17]
• CA1 Carbonic anhydrase 1 (EC 4.2.1.1) (Carbonic anhydrase I) (Carbonate dehydratase I) (CA-I) ^{[16] [17] [20]}

References

1. GRCh38: Ensembl release 89: ENSG00000095906 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000039183 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nakashima H, Grahovac MJ, Mazzarella R, et al. (1999). "Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome 17, and Nubp1, mapped to chromosome 16--establish a new gene family of nucleotide-binding proteins in eukaryotes". Genomics. 60 (2): 152–60. doi:10.1006/geno.1999.5898. PMID   10486206.
6. Stehling O, Netz DJ, Niggemeyer B, et al. (2008). "Human Nbp35 is essential for both cytosolic iron-sulfur protein assembly and iron homeostasis". Mol. Cell. Biol. 28 (17): 5517–28. doi:10.1128/MCB.00545-08. PMC   2519719 . PMID   18573874.
7. Netz DJ, Pierik AJ, Stümpfig M, Mühlenhoff U, Lill R (2007). "The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol". Nat Chem Biol. 3 (5): 278–86. doi:10.1038/nchembio872. PMID   17401378.
8. Hausmann A, Aguilar Netz DJ, Balk J, Pierik AJ, Mühlenhoff U, Lill R (2005). "The eukaryotic P-loop NTPase Nbp35: an essential component of the cytosolic and nuclear iron-sulfur protein assembly machinery". Proc Natl Acad Sci U S A. 102 (9): 3266–71. Bibcode:2005PNAS..102.3266H. doi: 10.1073/pnas.0406447102 . PMC   552912 . PMID   15728363.
9. Motallebi-Veshareh M, Rouch DA, Thomas CM (1990). "A family of ATPases involved in active partitioning of diverse bacterial plasmids". Mol. Microbiol. 4 (9): 1455–63. doi:10.1111/j.1365-2958.1990.tb02056.x. PMID   2149583. S2CID   40829252.
10. Balk J, Aguilar Netz DJ, Tepper K, Pierik AJ, Lill R (2005). "The essential WD40 protein Cia1 is involved in a late step of cytosolic and nuclear iron-sulfur protein assembly". Mol Cell Biol. 25 (24): 10833–41. doi:10.1128/MCB.25.24.10833-10841.2005. PMC   1316972 . PMID   16314508.
11. Rutherford JC, Ojeda L, Balk J, Mühlenhoff U, Lill R, Winge DR (2005). "Activation of the iron regulon by the yeast Aft1/Aft2 transcription factors depends on mitochondrial but not cytosolic iron-sulfur protein biogenesis". J Biol Chem. 280 (11): 10135–40. doi: 10.1074/jbc.M413731200 . PMID   15649888.
12. Balk J, Pierik AJ, Aguilar Netz DJ, Mühlenhoff U, Lill R (2005). "Nar1p, a conserved eukaryotic protein with similarity to Fe-only hydrogenases, functions in cytosolic iron-sulphur protein biogenesis". Biochem Soc Trans. 33 (Pt.1): 86–9. doi:10.1042/BST0330086. PMID   15667273. Archived from the original on 2012-03-12. Retrieved 2010-12-23.
13. Yarunin A, Panse VG, Petfalski E, Dez C, Tollervey D, Hurt EC (2005). "Functional link between ribosome formation and biogenesis of iron-sulfur proteins". EMBO J. 24 (3): 580–8. doi:10.1038/sj.emboj.7600540. PMC   548649 . PMID   15660135.
14. Roy A, Solodovnikova N, Nicholson T, et al. (2003). "A novel eukaryotic factor for cytosolic Fe-S cluster assembly". EMBO J. 22 (18): 4826–35. doi:10.1093/emboj/cdg455. PMC   212722 . PMID   12970194.
15. Okuno T, Yamabayashi H, Kogure K (2010). "Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins". Mol Biol Rep. 37 (3): 1165–8. doi:10.1007/s11033-009-9477-7. PMID   19263241. S2CID   998955.
16. Bignell C, Thomas CM (2001). "The bacterial ParA-ParB partitioning proteins". J Biotechnol. 91 (2): 1–34. doi:10.1016/S0168-1656(01)00293-0. ISSN   0168-1656. PMID   11522360.
17. Fukushima K, Ogawa H, Takahashi K, Naito H, Funayama Y, Kitayama T, Yonezawa H, Sasaki I (2003). "Non-pathogenic bacteria modulate colonic epithelial gene expression in germ-free mice". Scand J Gastroenterol. 38 (6): 626–34. doi:10.1080/00365510310000376. ISSN   0036-5521. PMID   12825871. S2CID   25927173.
18. Kohbushi H, Nakai Y, Kikuchi S, Yabe T, Hori H, Nakai M (2009). "Arabidopsis cytosolic Nbp35 homodimer can assemble both [2Fe-2S] and [4Fe-4S] clusters in two distinct domains". Biochem Biophys Res Commun. 378 (4): 810–5. doi:10.1016/j.bbrc.2008.11.138. PMID   19084504.
19. Bych K, Netz DJ, Vigani G, Bill E, Lill R, Pierik AJ, Balk J (2008). "The essential cytosolic iron-sulfur protein Nbp35 acts without Cfd1 partner in the green lineage". J Biol Chem. 283 (51): 35797–804. doi: 10.1074/jbc.M807303200 . hdl: 2318/1655385 . PMID   18957412.
20. "Entrez Gene: UniGene Hs.256549 Nucleotide binding protein 2 (MinD homolog, E. coli) (NUBP2)".
21. "Entrez Gene: NUCLEOTIDE-BINDING PROTEIN 2; NUBP2 CYTOSOLIC FE-S CLUSTER DEFICIENT 1, S. CEREVISIAE, HOMOLOG OF; CFD1 Gene map locus: 16p13.3".
22. Kargul GJ, Nagaraja R, Shimada T, Grahovac MJ, Lim MK, Nakashima H, Waeltz P, Ma P, Chen E, Schlessinger D, Ko MS (2000). "Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA". Genome Res. 10 (7): 916–23. doi:10.1101/gr.10.7.916. PMC   310918 . PMID   10899141.
23. Sano Y, Shimada T, Nakashima H, Nicholson RH, Eliason JF, Kocarek TA, Ko MS (2001). "Random monoallelic expression of three genes clustered within 60 kb of mouse t complex genomic DNA". Genome Res. 11 (11): 1833–41. doi:10.1101/gr.194301. PMC   311134 . PMID   11691847.
24. Christodoulou A, Lederer CW, Surrey T, Vernos I, Santama N (2006). "Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication". J Cell Sci. 119 (Pt 10): 2035–47. doi:10.1242/jcs.02922. PMID   16638812. S2CID   1004740.

Further reading

• Guey LT, GarcÃa-Closas M, Murta-Nascimento C, et al. (2010). "Genetic susceptibility to distinct bladder cancer subphenotypes". Eur. Urol. 57 (2): 283–92. doi:10.1016/j.eururo.2009.08.001. PMC   3220186 . PMID   19692168.
• Roy A, Solodovnikova N, Nicholson T, et al. (2003). "A novel eukaryotic factor for cytosolic Fe-S cluster assembly". EMBO J. 22 (18): 4826–35. doi:10.1093/emboj/cdg455. PMC   212722 . PMID   12970194.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Bonaldo MF, Lennon G, Soares MB (1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi: 10.1101/gr.6.9.791 . PMID   8889548.
• Shen M, Vermeulen R, Rajaraman P, et al. (2009). "Polymorphisms in innate immunity genes and lung cancer risk in Xuanwei, China". Environ. Mol. Mutagen. 50 (4): 285–90. Bibcode:2009EnvMM..50..285S. doi:10.1002/em.20452. PMC   2666781 . PMID   19170196.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Hosgood HD, Menashe I, He X, et al. (2009). "PTEN identified as important risk factor of chronic obstructive pulmonary disease". Respir Med. 103 (12): 1866–70. doi:10.1016/j.rmed.2009.06.016. PMC   2783799 . PMID   19625176.
• Hosgood HD, Menashe I, Shen M, et al. (2008). "Pathway-based evaluation of 380 candidate genes and lung cancer susceptibility suggests the importance of the cell cycle pathway". Carcinogenesis. 29 (10): 1938–43. doi:10.1093/carcin/bgn178. PMC   2722857 . PMID   18676680.