NotI

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NotI is a restriction enzyme isolated from the bacterium Nocardia otitidis . [1]

Recognition sequence

Palindromic recognition sequence of NotI: [1] 

5'-GCGGCCGC-3' 3'-CGCCGGCG-5'

The ends generated by NotI digest:

5'---GC     GGCCGC---3' 3'---CGCCGG     CG---5'

References

  1. 1 2 Biolabs, New England. "NotI | NEB". www.neb.com. Retrieved 2016-05-08.


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