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TaqI is a restriction enzyme isolated from the bacterium Thermus aquaticus in 1978. [1] It has a recognition sequence of
5'TCGA 3'AGCT
and makes the cut
5'---T CGA---3' 3'---AGC T---5'
Kary Banks Mullis was an American biochemist. In recognition of his role in the invention of the polymerase chain reaction (PCR) technique, he shared the 1993 Nobel Prize in Chemistry with Michael Smith and was awarded the Japan Prize in the same year. PCR became a central technique in biochemistry and molecular biology, described by The New York Times as "highly original and significant, virtually dividing biology into the two epochs of before PCR and after PCR."
The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA sufficiently to enable detailed study. PCR was invented in 1983 by American biochemist Kary Mullis at Cetus Corporation. Mullis and biochemist Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993.
In biochemistry, a polymerase is an enzyme that synthesizes long chains of polymers or nucleic acids. DNA polymerase and RNA polymerase are used to assemble DNA and RNA molecules, respectively, by copying a DNA template strand using base-pairing interactions or RNA by half ladder replication.
The year 1969 in science and technology involved some significant events, listed below.
Deinococcota is a phylum of bacteria with a single class, Deinococci, that are highly resistant to environmental hazards, also known as extremophiles. These bacteria have thick cell walls that give them gram-positive stains, but they include a second membrane and so are closer in structure to those of gram-negative bacteria.
DNA polymerase I is an enzyme that participates in the process of prokaryotic DNA replication. Discovered by Arthur Kornberg in 1956, it was the first known DNA polymerase. It was initially characterized in E. coli and is ubiquitous in prokaryotes. In E. coli and many other bacteria, the gene that encodes Pol I is known as polA. The E. coli Pol I enzyme is composed of 928 amino acids, and is an example of a processive enzyme — it can sequentially catalyze multiple polymerisation steps without releasing the single-stranded template. The physiological function of Pol I is mainly to support repair of damaged DNA, but it also contributes to connecting Okazaki fragments by deleting RNA primers and replacing the ribonucleotides with DNA.
Thermus aquaticus is a species of bacteria that can tolerate high temperatures, one of several thermophilic bacteria that belong to the Deinococcota phylum. It is the source of the heat-resistant enzyme Taq DNA polymerase, one of the most important enzymes in molecular biology because of its use in the polymerase chain reaction (PCR) DNA amplification technique.
Taq or TAQ may refer to:
Thermus is a genus of thermophilic bacteria. It is one of several bacteria belonging to the Deinococcota phylum. Thermus species can be distinguished from other genera in the family Thermaceae as well as all other bacteria by the presence of eight conserved signature indels (CSIs) found in proteins such as adenylate kinase and replicative DNA helicase as well as 14 conserved signature proteins (CSPs) that are exclusively shared by members of this genus.
Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by Chien et al. in 1976. Its name is often abbreviated to Taq or Taq pol. It is frequently used in the polymerase chain reaction (PCR), a method for greatly amplifying the quantity of short segments of DNA.
Krogh's principle states that "for such a large number of problems there will be some animal of choice, or a few such animals, on which it can be most conveniently studied." This concept is central to those disciplines of biology that rely on the comparative method, such as neuroethology, comparative physiology, and more recently functional genomics.
TaqMan probes are hydrolysis probes that are designed to increase the specificity of quantitative PCR. The method was first reported in 1991 by researcher Kary Mullis at Cetus Corporation, and the technology was subsequently developed by Hoffmann-La Roche for diagnostic assays and by Applied Biosystems for research applications.
The prokaryotic small ribosomal subunit, or 30S subunit, is the smaller subunit of the 70S ribosome found in prokaryotes. It is a complex of the 16S ribosomal RNA (rRNA) and 19 proteins. This complex is implicated in the binding of transfer RNA to messenger RNA (mRNA). The small subunit is responsible for the binding and the reading of the mRNA during translation. The small subunit, both the rRNA and its proteins, complexes with the large 50S subunit to form the 70S prokaryotic ribosome in prokaryotic cells. This 70S ribosome is then used to translate mRNA into proteins.
The history of the polymerase chain reaction (PCR) has variously been described as a classic "Eureka!" moment, or as an example of cooperative teamwork between disparate researchers. Following is a list of events before, during, and after its development:
The versatility of polymerase chain reaction (PCR) has led to modifications of the basic protocol being used in a large number of variant techniques designed for various purposes. This article summarizes many of the most common variations currently or formerly used in molecular biology laboratories; familiarity with the fundamental premise by which PCR works and corresponding terms and concepts is necessary for understanding these variant techniques.
Thomas Dale Brock was an American microbiologist known for his discovery of hyperthermophiles living in hot springs at Yellowstone National Park. In the late 1960s, Brock discovered high-temperature bacteria living in the Great Fountain region of Yellowstone, and with his colleague Hudson Freeze, they isolated a sample which they named Thermus aquaticus. "Life at High Temperatures", a 1967 article summarizing his research, was published in the journal Science and led to the study of extremophiles, organisms that live in extreme environments. By 1976, T. aquaticus was found useful for artificially amplifying DNA segments. Brock's discoveries led to great progress in biology, contributed to new developments in medicine and agriculture, and helped create the new field of biotechnology.
Carboxypeptidase Taq is an enzyme. This enzyme catalyses the following chemical reaction
Aqualysin 1 is an enzyme. This enzyme catalyses the following chemical reaction
Alicyclobacillus acidocaldarius is a species of Gram positive, strictly aerobic, bacterium. The bacteria are acidophilic, thermophilic, and produce endospores. The first identified strains of A. acidocaldarius were from geysers in Yellowstone National Park and fumerole soil in Hawaii Volcano National Park. The species was originally classified as Bacillus acidocaldarius in 1971, but further 16S rRNA studies found that the species belonged in the newly created genus Alicyclobacillus. The species name is derived from the Latin acidum (acid) and caldarius, referring to the acidic and high temperature environments from which it was first isolated. Thomas D. Brock was one of the researchers who first categorized the species; his discovery of Thermus aquaticus allowed for other researchers to discover Taq polymerase and polyermase chain reaction (PCR).
Extremophiles in biotechnology is the application of organisms that thrive in extreme environments to biotechnology.
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