List of restriction enzyme cutting sites: S

Last updated January 20, 2023

Legend of nucleobases
Code	Nucleotide represented
A	Adenine (A)
C	Cytosine (C)
G	Guanine (G)
T	Thymine (T)
N	A, C, G or T
M	A or C
R	A or G
W	A or T
Y	C or T
S	C or G
K	G or T
H	A, C or T
B	C, G or T
V	A, C or G
D	A, G or T

This article contains a list of the most studied restriction enzymes whose names start with S. It contains approximately 130 enzymes. The following information is given:

Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature ^[1]^[2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action^[3]^[4].
Source: Organism that naturally produces the enzyme.
Recognition sequence : Sequence of DNA recognized by the enzyme and to which it specifically binds.
Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site^[5]^[6].
Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:

as in this not listed enzyme:

EcoR70I

Whole list navigation

Restriction enzymes

S

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

SacI ^[7] Streptomyces achromogenes 5' GAGCTC
3' CTCGAG 5' ---GAGCT C--- 3'
3' ---C TCGAG--- 5'

SacII Streptomyces achromogenes 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5' Cfr42I

SacNI Streptomyces achromogenes N-J-H 5' GRGCYC
3' CYCGRG 5' ---GRGCY C--- 3'
3' ---C YCGRG--- 5'

SalI ^[7] Streptomyces albus 5' GTCGAC
3' CAGCTG 5' ---G TCGAC--- 3'
3' ---CAGCT G--- 5'

SalPI Streptomyces albus 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' ApiI, BspMAI, CstI, Ecl37kI, Ecl2zI, Psp23I, PstI, Srl5DI, YenI

SanDI Streptomyces sp. 5' GGGWCCC
3' CCCWGGG 5' ---GG GWCCC--- 3'
3' ---CCCWG GG--- 5'

SapI Saccharopolyspora sp. 5' GCTCTTC
3' CGAGAAG 5' ---GCTCTTCN NNN--- 3'
3' ---CGAGAAGNNNN --- 5'

SarI Streptomyces aureomonopodiales 5' AGGCCT
3' TCCGGA 5' ---AGG CCT--- 3'
3' ---TCC GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, Sru30DI, SseBI, SteI, StuI

SatI Staphylococcus arlettae RFL832 5' GCNGC
3' CGNCG 5' ---GC NGC--- 3'
3' ---CGN CG--- 5'

SauI Streptomyces aureofaciens IKA 18/4 5' CCTNAGG
3' GGANTCC 5' ---CC TNAGG--- 3'
3' ---GGANT CC--- 5' AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI

Sau96I Staphylococcus aureus PS96 5' GGNCC
3' CCNGG 5' ---G GNCC--- 3'
3' ---CCNG G--- 5' AsuI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, UnbI

Sau3239I Streptomyces aureofaciens 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI

Sau3AI 2REU Staphylococcus aureus 3A 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bce243I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I

SauBMKI Streptomyces aureofaciens BM-K 5' GCCGGC
3' CGGCCG 5' ---GCC GGC--- 3'
3' ---CGG CCG--- 5'

SauHPI Streptomyces aureofaciens R8/26 5' GCCGGC
3' CGGCCG 5' ---GCC GGC--- 3'
3' ---CGG CCG--- 5'

SauLPI Streptomyces aureofaciens B-96 5' GCCGGC
3' CGGCCG 5' ---GCC GGC--- 3'
3' ---CGG CCG--- 5'

SauLPII Streptomyces aureofaciens B-96 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI

SauMI Staphylococcus aureus M 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' BscFI, BspAI, BstKTI, ChaI, HacI, MkrAI, RalF40I, SsiAI

SauNI Streptomyces aureofaciens NMU 5' GCCGGC
3' CGGCCG 5' ---GCC GGC--- 3'
3' ---CGG CCG--- 5'

SauSI Streptomyces aureofaciens 16 5' GCCGGC
3' CGGCCG 5' ---GCC GGC--- 3'
3' ---CGG CCG--- 5'

SbfI Streptomyces sp. BF-61 5' CCTGCAGG
3' GGACGTCC 5' ---CCTGCA GG--- 3'
3' ---GG ACGTCC--- 5'

Sbi68I Streptomyces bikiniensis JAM68 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI

Sbo13I Shigella boydii 13 5' TCGCGA
3' AGCGCT 5' ---TCG CGA--- 3'
3' ---AGC GCT--- 5'

SbvI Streptococcus bovis II/1 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

ScaI ^[7]

Streptomyces caespitosus

5' AGTACT
3' TCATGA

5' ---AGT ACT--- 3'
3' ---TCA TGA--- 5'

Acc113I, AssI,	BmcAI, Bpa34I,
DpaI, Eco255I, RflFII, ZrmI

SceIII Saccharomyces cerevisiae 5' GCCGGC
3' CGGCCG 5' ---G CCGGC--- 3'
3' ---CGGCC G--- 5'

SchI Staphylococcus cohnii Lki 19-320 5' GAGTC
3' CTCAG 5' ---GAGTCN₄N --- 3'
3' ---CTCAGN₄N --- 5'

SchZI Streptomyces chusanensis ZS-2 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

SciI Streptoverticillium cinnamoneum 5' CTCGAG
3' GAGCTC 5' ---CTC GAG--- 3'
3' ---GAG CTC--- 5' BspAAI, BstVI, MlaAI, PaeR7I, SauLPII, SlaI, Sol10179I, XhoI

SciNI Spiroplasma citri ASP2 5' GCGC
3' CGCG 5' ---G CGC--- 3'
3' ---CGC G--- 5' BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI

ScrFI Streptococcus cremoris F 5' CCNGG
3' GGNCC 5' ---CC NGG--- 3'
3' ---GGN CC--- 5'

SdaI 2IXS Streptomyces diastaticus Ng7-324 5' CCTGCAGG
3' GGACGTCC 5' ---CCTGCA GG--- 3'
3' ---GG ACGTCC--- 5'

SdiI Streptomyces diastaticus 5' GGCCN₅GGCC
3' CCGGN₅CCGG 5' ---GGCCNNNN NGGCC--- 3'
3' ---CCGGN NNNNCCGG--- 5'

SduI Streptococcus durans RFL3 5' GDGCHC
3' CHCGDG 5' ---GDGCH C--- 3'
3' ---C HCGDG--- 5' AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII

SecI Synechocystis sp. 6701 5' CCNNGG
3' GGNNCC 5' ---C CNNGG--- 3'
3' ---GGNNC C--- 5'

SelI Synechococcus elongatus 5' CGCG
3' GCGC 5' --- CGCG--- 3'
3' ---GCGC --- 5' AccII, Bsh1236I, Bsp123I, Bsp50I, BstFNI, BstUI, BtkI, Csp68KVI

SenPT16I Salmonella enteritidis PT16 5' CGGCCG
3' GCCGGC 5' ---C GGCCG--- 3'
3' ---GCCGG C--- 5' AaaI, BseX3I, BstZI, EagI, EclXI, Eco52I, XmaIII

SenPT14bI Salmonella enteritidis PT14b 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

SepI Staphylococcus epidermidis 5' ATGCAT
3' TACGTA 5' ---ATGCA T--- 3'
3' ---T ACGTA--- 5' BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SspD5II, Zsp2I

SexAI Streptomyces exfoliatus 5' ACCWGGT
3' TGGWCCA 5' ---A CCWGGT--- 3'
3' ---TGGWCC A--- 5'

SexBI Streptomyces exfoliatus 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

SexCI Streptomyces exfoliatus 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

SfaI Streptococcus faecalis zymogenes 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

SfaNI Streptococcus faecalis ND547 5' GCATC
3' CGTAG 5' ---GCATCN₄N NNNN--- 3'
3' ---CGTAGN₄NNNNN --- 5'

SfcI Streptococcus faecium 5' CTRYAG
3' GAYRTC 5' ---C TRYAG--- 3'
3' ---GAYRT C--- 5'

SfeI Streptococcus faecalis 5' CTRYAG
3' GAYRTC 5' ---C TRYAG--- 3'
3' ---GAYRT C--- 5'

SfiI 2F03 Streptomyces fimbriatus 5' GGCCN₅GGCC
3' CCGGN₅CCGG 5' ---GGCCNNNN NGGCC--- 3'
3' ---CCGGN NNNNCCGG--- 5'

SflI Streptoverticillium flavopersicum 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' ApiI, BspMAI, BsuBI, CfrA4I, Psp23I, PstI, Sag23I, Srl5DI, YenI

SfoI Serratia fonticola 5' GGCGCC
3' CCGCGG 5' ---GGC GCC--- 3'
3' ---CCG CGG--- 5'

Sfr274I Streptomyces fradiae 274 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliI

Sfr303I Streptomyces fradiae 303 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

SfuI Streptomyces fulvissimus 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' AsuII, BimI, Bsp119I, CbiI, Csp68KII, PlaII, Ssp1I, SviI

SgfI

Streptomyces griseoruber

5' GCGATCGC
3' CGCTAGCG

5' ---GCGAT CGC--- 3'
3' ---CGC TAGCG--- 5'

AsiSI,

RgaI, SfaAI

SgrAI 3DPG 3N7B 3N78 3MGY 3MG6 Streptomyces griseus 5' CRCCGGYG
3' GYGGCCRC 5' ---CR CCGGYG--- 3'
3' ---GYGGCC RC--- 5'

SgrBI Streptomyces griseus 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

SimI Staphylococcus intermedius 6H 5' GGGTC
3' CCCAG 5' ---GG GTC--- 3'
3' ---CCCAG --- 5'

SinI Salmonella infantis 5' GGWCC
3' CCWGG 5' ---G GWCC--- 3'
3' ---CCWG G--- 5' Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11BI

SlaI Streptomyces lavendulae 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI

SleI Synechococcus leopoliensis 5' CCWGG
3' GGWCC 5' --- CCWGG--- 3'
3' ---GGWCC --- 5' AeuI, AglI, ApaORI, BspNI, Bst2I, EcoRII, SniI, SslI, SspAI, ZanI

Slu1777I Streptomyces lusitanus 1777 5' GCCGGC
3' CGGCCG 5' ---GCC GGC--- 3'
3' ---CGG CCG--- 5'

SmaI Serratia marcescens Sb 5' CCCGGG
3' GGGCCC 5' ---CCC GGG--- 3'
3' ---GGG CCC--- 5' AhyI, CfrJ4I, EaeAI, EclRI, Pac25I, PspAI, TspMI, XcyI, XmaI, XmaCI

SmiI Streptococcus milleri S 5' ATTTAAAT
3' TAAATTTA 5' ---ATTT AAAT--- 3'
3' ---TAAA TTTA--- 5'

SmiMI Sphingobacterium mizutae M 5' CAYN₄RTG
3' GTRN₄YAC 5' ---CAYNN NNRTG--- 3'
3' ---GTRNN NNYAC--- 5'

SmlI Stenotrophomonas maltophilia 5' CTYRAG
3' GARYTC 5' ---C TYRAG--- 3'
3' ---GARYT C--- 5'

SmuI Sphingobacterium multivorum RFL21 5' CCCGC
3' GGGCG 5' ---CCCGCNNNN NN--- 3'
3' ---GGGCGNNNNNN --- 5'

SmuEI Streptococcus mutans E 5' GGWCC
3' CCWGG 5' ---G GWCC--- 3'
3' ---CCWG G--- 5' Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11BI

SnaBI Sphaerotilus natans 5' TACGTA
3' ATGCAT 5' ---TAC GTA--- 3'
3' ---ATG CAT--- 5'

SniI Streptomyces niveus 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, MvaI, SspAI

SnoI Streptomyces novocastria 5' GTGCAC
3' CACGTG 5' ---G TGCAC--- 3'
3' ---CACGT G--- 5' Alw44I, ApaLI, VneI

SolI Streptoverticillium olivoverticillatum 5' GGATCC
3' CCTAGG 5' ---G GATCC--- 3'
3' ---CCTAG G--- 5' AccEBI, AliI, ApaCI, BamHI, Bce751I, CelI, OkrAI, SolI, SurI

Sol10179I Streptomyces olivochromogenes ST10179 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI

SpaHI Synechococus parietina 5' GCATGC
3' CGTACG 5' ---GCATG C--- 3'
3' ---C GTACG--- 5'

SpeI Sphaerotilus natans 5' ACTAGT
3' TGATCA 5' ---A CTAGT--- 3'
3' ---TGATC A--- 5' AhII, AclNI, BcuI

SphI ^[7] Streptomyces phaeochromogenes 5' GCATGC
3' CGTACG 5' ---GCATG C--- 3'
3' ---C GTACG--- 5'

SplI Spirulina platensis 5' CGTACG
3' GCATGC 5' ---C GTACG--- 3'
3' ---GCATG C--- 5'

SpmI Sphingobacterium multivorum RFL21 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BanIII, BbvAII, BdiI, BscI, Bsa29I, BspJI, ClaI, LcaI, PgaI, Ssp27144I

SpoI Salmonella potsdam 5' TCGCGA
3' AGCGCT 5' ---TCG CGA--- 3'
3' ---AGC GCT--- 5'

SpuI Streptomyces pulveraceus 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

SrfI Streptomyces sp. 5' GCCCGGGC
3' CGGGCCCG 5' ---GCCC GGGC--- 3'
3' ---CGGG CCCG--- 5'

SrlI Selenomonas ruminantium lactilytica 84 5' GCCGGC
3' CGGCCG 5' ---G CCGGC--- 3'
3' ---CGGCC G--- 5'

Srl5DI Selenomonas ruminantium 5 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' AliAJI, Asp713I, BspMAI, BsuBI, CfrA4I, Pfl21I, PstI, SflI, Srl5DI

Srl32DII Selenomonas ruminantium 32 5' GAATTC
3' CTTAAG 5' ---G AATTC--- 3'
3' ---CTTAA G--- 5'

Srl55DI Selenomonas ruminantium lactilytica 55 5' GAATTC
3' CTTAAG 5' ---G AATTC--- 3'
3' ---CTTAA G--- 5'

Srl56DI Selenomonas ruminantium 56 5' CTRYAG
3' GAYRTC 5' ---C TRYAG--- 3'
3' ---GAYRT C--- 5'

SruI Selenomonas ruminantium 18D 5' TTTAAA
3' AAATTT 5' ---TTT AAA--- 3'
3' ---AAA TTT--- 5' AhaIII, DraI, PauAII

Sru4DI Selenomonas ruminantium 5' ATTAAT
3' TAATTA 5' ---AT TAAT--- 3'
3' ---TAAT TA--- 5' AseI, AsnI, BpoAI, PshBI, VspI

Sru30DI Selenomonas ruminantium 30 5' AGGCCT
3' TCCGGA 5' ---AGG CCT--- 3'
3' ---TCC GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, SseBI, SteI, StuI

SsbI Streptomyces scabies 5' AAGCTT
3' TTCGAA 5' ---A AGCTT--- 3'
3' ---TTCGA A--- 5'

SscL1I Staphylococcus sp. L1 5' GANTC
3' CTNAG 5' ---G ANTC--- 3'
3' ---CTNA G--- 5'

Sse9I Sporosarcina sp. 9 5' AATT
3' TTAA 5' --- AATT--- 3'
3' ---TTAA --- 5'

Sse232I Streptomyces sp. RH232 5' CGCCGGCG
3' GCGGCCGC 5' ---CG CCGGCG--- 3'
3' ---GCGGCC GC--- 5'

Sse1825I Streptomyces sp. 5' GGGWCCC
3' CCCWGGG 5' ---GG GWCCC--- 3'
3' ---CCCWG GG--- 5'

Sse8387I Streptomyces sp. 8387 5' CCTGCAGG
3' GGACGTCC 5' ---CCTGCA GG--- 3'
3' ---GG ACGTCC--- 5'

Sse8647I Streptomyces sp. 5' AGGWCCT
3' TCCWGGA 5' ---AG GWCCT--- 3'
3' ---TCCWG GA--- 5'

SseAI Streptomyces sp. 5' GGCGCC
3' CCGCGG 5' ---GG CGCC--- 3'
3' ---CCGC GG--- 5'

SseBI Streptomyces sp. 5' AGGCCT
3' TCCGGA 5' ---AGG CCT--- 3'
3' ---TCC GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, Sru30DI, SteI, StuI

SshAI Salmonella shikmonah 5' CCTNAGG
3' GGANTCC 5' ---CC TNAGG--- 3'
3' ---GGANT CC--- 5' AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI

SsiI Staphylococcus sciuri RFL1 5' CCGC
3' GGCG 5' ---C CGC--- 3'
3' ---GGC G--- 5' AciI

SsiAI Staphylococcus sp. A 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' BscFI, BspAI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI

SsiBI Staphylococcus sp. B 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' BscFI, BspFI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI

SslI Streptococcus salivarius thermophilus 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, EcoRII, MvaI

SsoI Shigella sonnei 47 5' GAATTC
3' CTTAAG 5' ---G AATTC--- 3'
3' ---CTTAA G--- 5'

SsoII Shigella sonnei 47 5' CCNGG
3' GGNCC 5' --- CCNGG--- 3'
3' ---GGNCC --- 5'

SspI Sphaerotilus sp. 5' AATATT
3' TTATAA 5' ---AAT ATT--- 3'
3' ---TTA TAA--- 5'

Ssp1I Streptomyces sp. RFL1 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' AsuII, BimI, Bsp119I, CbiI, MlaI, PlaII, SfuI, SspRFI, SviI

Ssp4800I

Streptomyces sp. BMTU 4800

5' TGTACA
3' ACATGT

5' ---T GTACA--- 3'
3' ---ACATG T--- 5'

AauI,	BsmRI,	Bsp1407I,
BsrGI, BstAUI, SspBI

Ssp5230I Streptomyces sp. 5230 5' GACGTC
3' CTGCAG 5' ---GACGT C--- 3'
3' ---C TGCAG--- 5' AatII, ZraI

Ssp27144I Synechococcus sp. 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BanIII, BavCI, BbvAII, BscI, BspJI, BspOVII, BstNZ169I, ClaI, LcaI

SspAI Synechococcus sp. 5' CCWGG
3' GGWCC 5' --- CCWGG--- 3'
3' ---GGWCC --- 5' AeuI, AorI, Bse17I, BspNI, Bst2I, EcoRII, SniI, SslI, SspAI, ZanI

SspBI

Streptomyces sp.

5' TGTACA
3' ACATGT

5' ---T GTACA--- 3'
3' ---ACATG T--- 5'

AauI,	BsmRI,	Bsp1407I,
BsrGI, BstAUI, Ssp4800I

SspCI Streptomyces sp. 5' GCCGGC
3' CGGCCG 5' ---GCC GGC--- 3'
3' ---CGG CCG--- 5'

SspD5I Staphylococcus sp. D5 5' GGTGA
3' CCACT 5' ---GGTGAN₇N --- 3'
3' ---CCACTN₇N --- 5' AsuHPI, HphI

SspD5II Staphylococcus sp. D5 5' ATGCAT
3' TACGTA 5' ---ATGCA T--- 3'
3' ---T ACGTA--- 5' Csp68KIII, EcoT22I, Mph1103I, NsiI, PinBI, Ppu10I, SepI, Zsp2I

SspRFI Synechococcus sp. RF-1 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' AsuII, BimI, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I, SviI

SsrI Staphylococcus saprophyticus B6 5' GTTAAC
3' CAATTG 5' ---GTT AAC--- 3'
3' ---CAA TTG--- 5'

SstI Streptomyces stanford 5' GAGCTC
3' CTCGAG 5' ---GAGCT C--- 3'
3' ---C TCGAG--- 5'

SstII Streptomyces stanford 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

Sst12I Streptomyces sp. ST-12 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' Asp713I, BloHII, BspMAI, BsuBI, CfuII, Psp23I, PstI, SflI, Srl5DI

SteI Streptomyces tendae 5' AGGCCT
3' TCCGGA 5' ---AGG CCT--- 3'
3' ---TCC GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, StuI

SthI Salmonella thompson YY356 5' GGTACC
3' CCATGG 5' ---G GTACC--- 3'
3' ---CCATG G--- 5' Acc65I, AhaB8I, Asp718I, KpnI,

Sth117I Streptococcus thermophilus ST117 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' ApaORI, BseBI, BsiLI, BstNI, BstOI, Bst2UI, EcoRII, MvaI, SspAI

Sth132I Streptococcus thermophilus 5' CCCG
3' GGGC 5' ---CCCGNNNN NNNN--- 3'
3' ---GGGCNNNNNNNN --- 5'

Sth134I Streptococcus thermophilus 134 5' CCGG
3' GGCC 5' ---C CGG--- 3'
3' ---GGC C--- 5'

Sth368I Streptococcus thermophilus CNRZ368 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' BscFI, BspFI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI

StrI Streptomyces thermodiastaticus 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI

StsI Streptococcus sanguis 54 5' GGATG
3' CCTAC 5' ---GGATGN₈NN NNNN--- 3'
3' ---CCTACN₈NNNNNN --- 5'

StuI ^[8] Streptomyces tubercidicus 5' AGGCCT
3' TCCGGA 5' ---AGG CCT--- 3'
3' ---TCC GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI

StyI Salmonella typhi 5' CCWWGG
3' GGWWCC 5' ---C CWWGG--- 3'
3' ---GGWWC C--- 5'

StyD4I Salmonella typhi D4 5' CCNGG
3' GGNCC 5' --- CCNGG--- 3'
3' ---GGNCC --- 5'

SuaI Sulfolobus acidocaldarius 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

SuiI Sulfolobus islandicus REN2HI 5' GCWGC
3' CGWCG 5' ---G CWGC--- 3'
3' --- CGWC G--- 5' AceI, ApeKI, Taq52I, TseI

SunI Synechococcus uniformis 5' CGTACG
3' GCATGC 5' ---C GTACG--- 3'
3' ---GCATG C--- 5'

SurI Sporosarcina ureae 2 5' GGATCC
3' CCTAGG 5' ---G GATCC--- 3'
3' ---CCTAG G--- 5' AccEBI, AliI, ApaCI, AsiI, BamHI, Bce751I, OkrAI, RspLKII, SolI

SviI Streptomyces violochromogenes 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' AsuII, Bpu14I, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I

SwaI Staphylococcus warneri 5' ATTTAAAT
3' TAAATTTA 5' ---ATTT AAAT--- 3'
3' ---TAAA TTTA--- 5'

Notes

↑ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
↑ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790 . PMID 12654995.
↑ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
↑ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
↑ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
↑ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916 . PMID 11557805.
1 2 3 4 Monty Krieger; Matthew P Scott; Matsudaira, Paul T.; Lodish, Harvey F.; Darnell, James E.; Lawrence Zipursky; Kaiser, Chris; Arnold Berk (2004). Molecular Cell Biology (5th ed.). New York: W.H. Freeman and Company. ISBN 0-7167-4366-3.
↑ Shimotsu H, Takahashi H, Saito H (November 1980). "A new site-specific endonuclease StuI from Streptomyces tubercidicus". Gene. 11 (3–4): 219–25. doi:10.1016/0378-1119(80)90062-1. PMID 6260571.

v t e Restriction modification system: restriction enzyme
Basic Concept	Isoschizomer Neoschizomer Isocaudomer
Recognition sequence 4bp	TaqI Sau3A AluI* HaeIII* DpnII NlaIII HpaII
Recognition sequence 5bp	EcoRII FokI
Recognition sequence 6bp	AaaI BglII PovII* EcoRV* EcoRI BamHI HindIII XbaI XhoI SacI PstI NdeI
Recognition sequence 8bp	NotI
Lists	A Ba–Bc Bd–Bp Bsa–Bso Bsp–Bss Bst–Bv C-D E-F G-K L-N O-R S T-Z
* means cleavage produces blunt ends

List of restriction enzyme cutting sites: S

Contents

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Restriction enzymes

S

Notes

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