Orthodenticle

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Orthodenticle (otd) is a homeobox gene found in Drosophila that regulates the development of anterior patterning, with particular involvement in the central nervous system function and eye development. [1] It is located on the X chromosome. [1] The gene is an ortholog of the human OTX1/OTX2 gene. [2]

Contents

Function

During embryonic Drosophila development, otd is required for the head and ventral midline to develop correctly. In the larval stage, otd is expressed in specific sac-like epithelial structures known as imaginal discs that later give rise to external structures of the head and thorax. Particularly, otd is required for the development of the dorsal region of the adult Drosophila head, that forms from the fusion of two eye-antennal discs. Distribution of the otd protein occurs along a concentration of the imaginal disc primordia for these head structures such that different levels of otd expression are required for differential mediolateral subdomains to be established. [3] In Drosophila, the function of otd as an essential factor in anterior pattern formation has been replaced by Bicoid, another homeodomain protein [4] which is involved in anterior patterning such that bcd mRNA is sequestered at the anterior pole of the mature oocyte. In the absence of bcd, all anterior structures of the embryo including the cephalic and thoracic segments fail to develop, and duplications of posterior structures are observed instead. [4]

Additionally, otd is involved in development of all eight photoreceptors of adult Drosophila in each of their ~700 individual eye units, known as ommatidia [5] and their proximal-distal distribution in the eye. [6] The two types of photoreceptors, outer (OPRs) and inner (IPRs), are distinguished through their function and anatomy such that OPRs have six neurons that respond to dim light conditions and are important for motion detection, while IPRs have two neurons that differentiate colour. [5] OPRs possess light-gathering apical surfaces called rhabdomeres, and otd is required for rhabdomere morphogenesis. Rapid expression of otd in all OPRs and IPRs is found following neuronal cell specification in the late larval stage and persists through photoreceptor differentiation. In later development of pupation through to adulthood, otd activates light detecting rhodopsin proteins Rh3 and Rh5 in IPRs while repressing Rh6 in OPRs.

Regulation

Embryo

In the anterior cap region of the syncytial blastoderm, which has non-segregated nuclei along the periphery of the blastoderm, bicoid in low concentrations is sufficient to activate otd expression through an enhancer located upstream of the otd gene. [7] [8] [9] Later, in the cellular blastoderm, which has all nuclei along the periphery separated in individual cells with their own cell membrane, otd expression is limited to a dorsal and anterior band due to repression of bicoid by torso, hunchback, and dorsal. [7] [8] [9] Torso and hunchback expression represses otd expression in the anterior most region of the blastoderm while dorsal expression represses expression in the ventral region. [1] [7] Groucho-independent repression of otd by runt, a pair-rule gene, has also been observed in the anterior and posterior regions of the syncytial and cellular blastoderm. [10]

Ocelliless

Ocelliless (oc) is a general mutation in the otd gene locus in Drosophila , [11] which results in a loss of ocelli: the three photoreceptor organs located on the anterior region of Drosophila heads [1] Two alleles oc1 and ocγa1, are caused by lesions downstream of the last exon in otd which showed enhancer activity. [11] Another characteristic of the oc phenotype is the abnormal or deleted bristles in the ocellus region, [12] which relates back to the wild type otd gene’s role in mediating anterior patterning. [4] The same oc phenotype is found in loss-of-function epidermal growth factor receptor (EGFR) alleles and RNAi knockdown pointed (pnt) mutants [13]

The effect of oc on ocelli can be complemented by otduvi, another hypomorphic allele of the otd gene. otduvi/oc heterozygotes shows no ocellar phenotype or photoreceptor (R cell) phenotype, a phenotype that is related with otduvi allele. [6]

The Ocelliless mutant phenotype is not limited to loss of ocelli only. It has a role in disrupting oogenesis, where the inner and outer layer of the chorion in oc mutant eggs separate from one another leaving space for debris that is not seen in wild type otd, and disrupting reproductive capabilities, where female flies homozygous for the oc mutation are sterile. [14]

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References

  1. 1 2 3 4 Finkelstein R, Smouse D, Capaci TM, Spradling AC, Perrimon N (July 1990). "The orthodenticle gene encodes a novel homeo domain protein involved in the development of the Drosophila nervous system and ocellar visual structures". Genes & Development. 4 (9): 1516–1527. doi: 10.1101/gad.4.9.1516 . PMID   1979296.
  2. ↑ Leuzinger S, Hirth F, Gerlich D, Acampora D, Simeone A, Gehring WJ, Finkelstein R, Furukubo-Tokunaga K, Reichert H (April 1998). "Equivalence of the fly orthodenticle gene and the human OTX genes in embryonic brain development of Drosophila". Development. 125 (9): 1703–1710. doi:10.1242/dev.125.9.1703. PMID   9521908.
  3. ↑ Royet J, Finkelstein R (November 1995). "Pattern formation in Drosophila head development: the role of the orthodenticle homeobox gene" (PDF). Development. 121 (11): 3561–72. doi:10.1242/dev.121.11.3561. PMID   8582270.
  4. 1 2 3 Datta RR, Ling J, Kurland J, Ren X, Xu Z, Yucel G, Moore J, Shokri L, Baker I, Bishop T, Paolo S, Levina R, Bulyk ML, Johnstron Jr RJ, Small S (May 2018). "A feed-forward relay integrates the regulatory activities of Bicoid and Orthodenticle via sequential binding to suboptimal sites". Genes & Development. 32 (9–10): 723–736. doi:10.1101/gad.311985.118. PMC   6004077 . PMID   29764918. S2CID   21727027.
  5. 1 2 McDonald EC, Xie B, Workman M, Charlton-Perkins M, Terrel DA, Reischl J, Wimmer EA, Gebelein BA, Cook TA (November 2012). "Separable transcriptional regulatory domains within Otd control photoreceptor terminal differentiation events". Developmental Biology. 347 (1): 122–132. doi:10.1016/j.ydbio.2010.08.016. PMC   2969183 . PMID   20732315.
  6. 1 2 Vandendries ER, Johnson D, Reinke R (January 1996). "orthodenticle Is Required for Photoreceptor Cell Development in the Drosophila Eye". Developmental Biology. 173 (1): 243–255. doi: 10.1006/dbio.1996.0020 . PMID   8575625.
  7. 1 2 3 Gao Q, Wang Y, Finkelstein R (May 1996). "orthodenticle regulation during embryonic head development in Drosophila". Mechanisms of Development. 56 (1): 3–15. doi: 10.1016/0925-4773(96)00504-7 . PMID   8798143. S2CID   16431692.
  8. 1 2 Gao Q, Finkelstein R (September 1998). "Targeting gene e expression to the head: The drosophila orthodenticle gene is a direct target of the bicoid gene". Development. 125 (21): 4185–4193. doi:10.1242/dev.125.21.4185. PMID   9753673.
  9. 1 2 Mazumdar A, Mazumdar M (October 2022). "How one becomes many: Blastoderm cellularization in Drosophila melanogaster". BioEssays. 24 (11): 1012–1022. doi:10.1002/bies.10184. PMID   12386932. S2CID   45642558.
  10. ↑ Tsai CC, Krame SG, Gergen JP (December 1998). "Pair-rule gene runt restricts orthodenticle expression to the presumptive head of the drosophila embryo". Developmental Genetics. 23 (1): 35–44. doi:10.1002/(SICI)1520-6408(1998)23:1<35::AID-DVG4>3.0.CO;2-7. PMID   9706692.
  11. 1 2 Blanco J, Seimiya M, Pauli T, Reichert H, Gehring WJ (May 2009). "Wingless and Hedgehog signaling pathways regulate orthodenticle and eyes absent during ocelli development in Drosophila". Developmental Biology. 329 (1): 104–115. doi: 10.1016/j.ydbio.2009.02.027 . PMID   19268449.
  12. ↑ Fichelson P, Brigui A, Pichaud F (May 2012). "Orthodenticle and Kruppel homolog 1 regulate Drosophila photoreceptor maturation". Proceedings of the National Academy of Sciences. 109 (20): 7893–7898. Bibcode:2012PNAS..109.7893F. doi: 10.1073/pnas.1120276109 . PMC   3356647 . PMID   22547825.
  13. ↑ Jean-Guillaume CB, Kumar JP (May 2022). "Development of the ocellar visual system in Drosophila melanogaster". The FEBS Journal. 289 (23): 7411–7427. doi:10.1111/febs.16468. PMC   9805374 . PMID   35490409. S2CID   248477548.
  14. ↑ Johnson CC, King RC (December 1974). "Oogenesis in the ocelliless mutant of Drosophila melanogaster Meigen (Diptera: Drosophilidae)". International Journal of Insect Morphology and Embryology. 3 (3–4): 385–395. doi:10.1016/0020-7322(74)90032-4.
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