Paenibacillus vortex

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Paenibacillus vortex
Paenibacillus fig 1.tif
Figure 1: Colony organization of the P. vortex bacteria when grown on 15g/L peptone and 2.25% (w/v) agar for four days. The bright yellow dots are the vortices. The colonies were grown in a Petri dish size 8.8cm and stained with Coomassie dyes (Brilliant Blue). The colors were inverted to emphasize higher densities using the brighter shades of yellow.
Scientific classification
Domain:
Bacteria
Phylum:
Bacillota
Class:
Bacilli
Order:
Bacillales
Family:
Paenibacillaceae
Genus:
Paenibacillus
Binomial name
Paenibacillus vortex
Synonyms
Bacillus vortex
Ash et al. 1994

Paenibacillus vortex is a species of pattern-forming bacteria, first discovered in the early 1990s by Eshel Ben-Jacob's group at Tel Aviv University. [1] It is a social microorganism that forms colonies with complex and dynamic architectures. P. vortex is mainly found in heterogeneous and complex environments, such as the rhizosphere, the soil region directly influenced by plant roots.

Contents

The genus Paenibacillus comprises facultative anaerobic, endospore-forming bacteria originally included within the genus Bacillus and then reclassified as a separate genus in 1993. [2] Bacteria in the genus have been detected in a variety of environments such as: soil, water, vegetable matter, forage and insect larvae, as well as clinical samples. [3] [4] [5] [6] Paenibacillus spp., including P. vortex, produce extracellular enzymes that catalyze a variety of synthetic reactions in industrial, agricultural and medical applications. [7] [8] [9] Various Paenibacillus spp. also produce antimicrobial substances that can affect micro-organisms such as fungi, in addition to soil and plant pathogenic bacteria. [10] [11] [12]

Social Motility

Paenibacillus vortex possesses advanced social motility employing cell-cell attractive and repulsive chemotactic signalling and physical links. When grown on soft surfaces (e.g. agar), the collective motility is reflected by the formation of foraging swarms [13] that act as arms sent out in search of food. These swarms have an aversion to crossing each other’s trail and collectively change direction when food is sensed. The “swarming intelligence” P. vortex, is further marked by the fact that of the swarms can even split and reunite when detecting scattered patches of nutrients. [13]

Pattern Formation and Social Behaviors

Figure 2: Scanning electron microscope (SEM) observation of P. vortex illustrating a typical bacteria arrangement in the center of a vortex. Notable, that each individual bacterium is curved. Scale bar in is 5um. Vortex fig 2.tif
Figure 2: Scanning electron microscope (SEM) observation of P. vortex illustrating a typical bacteria arrangement in the center of a vortex. Notable, that each individual bacterium is curved. Scale bar in is 5µm.

P. vortex is a social microorganism: when grown on under growth conditions that mimic natural environments such as hard surfaces it forms colonies of 109-1012 cells with remarkably complex and dynamic architectures (Figure 1). [1] [14] [15] Being part of a large cooperative, the bacteria can better compete for food resources and be protected against antibacterial assaults. [13] [14] Under laboratory growth conditions, similar to other social bacteria, P. vortex colonies behave much like a multi-cellular organism, with cell differentiation and task distribution. [16] [17] [18] [19] P. vortex is marked by its ability to generate special aggregates of dense bacteria that are pushed forward by repulsive chemotactic signals sent from the cells at the back. [15] [20] [21] [22] [23] [24] These rotating aggregates termed vortices (Figure 2), pave the way for the colony to expand. The vortices serve as building blocks of colonies with special modular organization (Figure 1). Accomplishing such intricate cooperative ventures requires sophisticated cell-cell communication, [14] [19] [25] [26] [27] including semantic and pragmatic aspects of linguistics. [19] Communicating with each other using a variety of chemical signals, bacteria exchange information regarding population size, a myriad of individual environmental measurements at different locations, their internal states and their phenotypic and epigenetic adjustments. The bacteria collectively sense the environment and execute distributed information processing to glean and assess relevant information. [14] [19] [28] The information is then used by the bacteria for reshaping the colony while redistributing tasks and cell epigenetic differentiations, for collective decision-making and for turning on and off defense and offense mechanisms needed to thrive in competitive environments, faculties that can be perceived as social intelligence of bacteria. [19]

A typical colonial pattern generated by P. vortex when grown on 2.25% (w/v) agar with 2% (w/v) peptone in a 9 cm Petri dish. Paenibacillus vortex colony pattern.JPEG
A typical colonial pattern generated by P. vortex when grown on 2.25% (w/v) agar with 2% (w/v) peptone in a 9 cm Petri dish.

Genome Sequence of the Paenibacillus vortex

The genome sequence of the P. vortex [29] is now available [GenBank: ADHJ00000000). The genome was sequenced by a hybrid approach using 454 Life Sciences and Illumina, achieving a total of 289X coverage, with 99.8% sequence identity between the two methods. The sequencing results were validated using a custom designed Agilent microarray expression chip submitted to EMBL-EBI [ArrayExpress: E-MEXP-3019 which represented the coding and the non-coding regions. Analysis of the P. vortex genome revealed 6,437 open reading frames (ORFs) and 73 non-coding RNA genes. The analysis also unveiled the P. vortex potential to produce a wealth of enzymes and proteases as well as a great variety of antimicrobial substances that affect a wide range of microorganisms. The possession of these advanced defense and offense strategies render Paenibacillus vortex as a rich source of useful genes for agricultural, medical, industrial and biofuel applications.

Comparative Genomics and Social-IQ Score

Comparative genomic analysis revealed that bacteria successful in heterogeneous and competitive environments often contain extensive signal transduction and regulatory networks. [30] [31] [32] Detailed comparative genomic analysis with a dataset of 500 complete bacterial genomes revealed that P. vortex has the third highest number of signal transduction genes, slightly below two other Paenibacillus species, Paenibacillus sp. JDR-2 and Paenibacillus sp. Y412MC10. [29] The comparative genomic analysis further revealed that these three Paenibacillus species also have the highest “Social-IQ” score among all 500 sequenced bacteria, over 3 standard deviations higher than average. The score is based on the number of genes which afford bacteria abilities to communicate and process environmental information (two-component and transcription-factor genes), to make decisions and to synthesize offensive (toxic) and defensive (neutralizing) agents as needed during chemical warfare with other microorganisms. [29] Defined this way, the Social-IQ score provides a measure of the genome capacity for social intelligence, hence it helps realizing social intelligence of bacteria.

Related Research Articles

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<span class="mw-page-title-main">Microorganism</span> Microscopic living organism

A microorganism, or microbe, is an organism of microscopic size, which may exist in its single-celled form or as a colony of cells.

<span class="mw-page-title-main">Endospore</span> Protective structure formed by bacteria

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<i>Paenibacillus</i> Genus of bacteria

Paenibacillus is a genus of facultative anaerobic, endospore-forming bacteria, originally included within the genus Bacillus and then reclassified as a separate genus in 1993. Bacteria belonging to this genus have been detected in a variety of environments, such as: soil, water, rhizosphere, vegetable matter, forage and insect larvae, as well as clinical samples. The name reflects: Latin paene means almost, so the paenibacilli are literally "almost bacilli". The genus includes P. larvae, which causes American foulbrood in honeybees, P. polymyxa, which is capable of fixing nitrogen, so is used in agriculture and horticulture, the Paenibacillus sp. JDR-2 which is a rich source of chemical agents for biotechnology applications, and pattern-forming strains such as P. vortex and P. dendritiformis discovered in the early 90s, which develop complex colonies with intricate architectures as shown in the pictures:

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<span class="mw-page-title-main">Eshel Ben-Jacob</span>

Eshel Ben-Jacob, was a theoretical and experimental physicist at Tel Aviv University, holder of the Maguy-Glass Chair in Physics of Complex Systems, and Fellow of the Center for Theoretical Biological Physics (CTBP) at Rice University. During the 1980s he became a leader in the theory of self-organization and pattern formation in open systems, later extending this work to adaptive complex systems and biocomplexity. In the late 1980s, he turned to study of bacterial self-organization, He developed new pattern forming bacteria species, becoming a pioneer in the study of bacterial intelligence and social behaviors of bacteria.

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<span class="mw-page-title-main">Social motility</span>

Social motility describes the motile movement of groups of cells that communicate with each other to coordinate movement based on external stimuli. There are multiple varieties of each kingdom that express social motility that provides a unique evolutionary advantages that other species do not possess. This has made them lethal killers such as African trypanosomiasis, or Myxobacteria. These evolutionary advantages have proven to increase survival rate among socially motile bacteria whether it be the ability to evade predators or communication within a swarm to form spores for long term hibernation in times of low nutrients or toxic environments.

References

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