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Phagomimicry is a defensive behaviour of sea hares, in which the animal ejects a mixture of chemicals, which mimic food, and overwhelm the senses of their predator, giving the sea hare a chance to escape. [1] [2] [3] The typical defence response of the sea hare to a predator is to release two chemicals - ink from the ink gland and opaline from the opaline gland. While ink creates a dark, diffuse cloud in the water which disrupts the sensory perception of the predator by acting as a smokescreen and as a decoy, the opaline, which affects the senses dealing with feeding, causes the predator to instinctively attack the cloud of chemicals as if it were indeed food. [1] This ink is able to mimic food by having a high concentration of amino acids and other compounds that are normally found in food, and the attack behaviour of the predator allows the sea-hares the opportunity to escape. [4]
The inking behaviour exhibited in phagomimicry is in response to predator threat. Sea hares have many natural predators such as starfish, lobsters, and other crustaceans. When threatened by a predator, phagomimicry behaviour begins. An ink solution is released from both the opaline and ink glands individually, then the compounds mix in the mantle of the sea hare to form the ink mixture. When ink is released it creates a smoke-screen like defense mechanism allowing the sea hares time to escape while also affecting the olfactory and gustatory senses of their predator. [1] Predators are tricked into thinking that they have captured their prey due to the specific chemical composition of the ink released. This induces feeding behaviours in the predator, and again gives the sea hares a better chance of escaping predation.
The opaline gland is a structure resembling a bundle of grapes attached to a central canal which is composed of epithelial cells. Synthesis of the opaline substance happens in the opaline vesicles themselves, as there are only opaline vesicles and muscle cells in the opaline gland. [5] The gland is innervated by three separate motor neurons, and is composed of single large cells and vesicle cells, all of which have enlarged nucleus. These cells are inclosed in an external layer of muscle. When a sensory neuron detects a predator threat, dopamine is released onto one of the three motor neurons. The dopamine release causes a gland contraction, which then causes the expulsion of the opaline substance. [6]
The ink gland is smaller in size than the opaline gland, and is composed of two cell types: rough endoplasmic recticlum (RER) and granulate cells. The cells are surrounded by a layer of muscle, to contraction and expelling their contents. The RER is the formation site of the anti-predator protein, the granulate cells are for extra pigment storage. Pigment is dependent on the amount of red algae available to the sea hares, the higher the red algal consumption, the darker the colour of their ink. [7] This mixing can cause another chain of reactions between the compounds that have further implications on the effect that the ink secretion has on predators. [5]
Both the opaline and ink gland secrete different substances that when mixed together form the ink released during phagomimicry. The secretion is very acidic (ink having a pH of 4.9 and opaline having a pH of 5.8) and contains high levels of bioactive molecules that can serve as feeding stimulants, feeding deterrents, and aversive compounds. Feeding stimulants can be found in both the ink and opaline secretions in the form of amino acids (such as lysine and arginine), and serve to trick predators into thinking that the ink secretion is a food source. [8] [4] To induce the aversive feeding effects on predators the ink contains a compound from the opaline gland produced from the oxidation of L-lysine, which is then mixed in the mantle with the L-amino acid oxidase from the ink gland. [8] Together this compound, called escapin is secreted in the ink, and is a feeding deterrent. [9] The ink secretion can have a long-lasting effect on predators as chemical phagomimics can cause chemo-mechanosensory stimulation which overwhelms the sensory system and leads to confusion and eventually the cession of the attack. [7] [8]
The ink released from the ink gland is dark purple in colour, the colour depends on the type of algae consumed by the sea-hare. [7] The opaline ink is white in colour, and when it mixes with the ink gland ink they form a compound that suspends itself in the water (polidisperse suspension), creating a smoke-screen defence mechanism. [7] The particle density of the ink is similar to that of species such as cuttlefish, the particles range in size from 80–150 nm, with a density of 1.27 cm-3, which allows for the inks suspension in water [10] .
Chemical synapses are biological junctions through which neurons' signals can be sent to each other and to non-neuronal cells such as those in muscles or glands. Chemical synapses allow neurons to form circuits within the central nervous system. They are crucial to the biological computations that underlie perception and thought. They allow the nervous system to connect to and control other systems of the body.
The California sea hare is a species of sea slug in the sea hare family, Aplysiidae. It is found in the Pacific Ocean, off the coast of California in the United States and northwestern Mexico.
The clade Anaspidea, commonly known as sea hares, are medium-sized to very large opisthobranch gastropod molluscs with a soft internal shell made of protein. These are marine gastropod molluscs in the superfamilies Aplysioidea and Akeroidea.
Sea slug is a common name for some marine invertebrates with varying levels of resemblance to terrestrial slugs. Most creatures known as sea slugs are gastropods, i.e. they are sea snails that, over evolutionary time, have either entirely lost their shells or have seemingly lost their shells due to having a significantly reduced or internal shell. The name "sea slug" is often applied to nudibranchs and a paraphyletic set of other marine gastropods without apparent shells.
Aplysiidae is the only family in the superfamily Aplysioidea, within the clade Anaspidea. These animals are commonly called sea hares because, unlike most sea slugs, they are often quite large, and when they are underwater, their rounded body shape and the long rhinophores on their heads mean that their overall shape resembles that of a sitting rabbit or hare. Sea hares are however sea snails with shells reduced to a small plate hidden between the parapodia, and some species are extremely large. The Californian black sea hare, Aplysia vaccaria is arguably the largest living gastropod species, and is certainly the largest living heterobranch gastropod.
Aplysia is a genus of medium-sized to extremely large sea slugs, specifically sea hares, which are a kind of marine gastropod mollusk.
The olfactory mucosa is the neuroepithelialial mucosa lining the roof and upper parts of the septum and lateral wall of the nasal cavity which contains bipolar neurons of the primary receptor neurons of the olfactory pathway, as well as supporting cells. The neurons' dendrites project towards the nasal cavity while their axons ascend through the cribriform plate as the olfactory nerves.
The arcuate nucleus of the hypothalamus is an aggregation of neurons in the mediobasal hypothalamus, adjacent to the third ventricle and the median eminence. The arcuate nucleus includes several important and diverse populations of neurons that help mediate different neuroendocrine and physiological functions, including neuroendocrine neurons, centrally projecting neurons, and astrocytes. The populations of neurons found in the arcuate nucleus are based on the hormones they secrete or interact with and are responsible for hypothalamic function, such as regulating hormones released from the pituitary gland or secreting their own hormones. Neurons in this region are also responsible for integrating information and providing inputs to other nuclei in the hypothalamus or inputs to areas outside this region of the brain. These neurons, generated from the ventral part of the periventricular epithelium during embryonic development, locate dorsally in the hypothalamus, becoming part of the ventromedial hypothalamic region. The function of the arcuate nucleus relies on its diversity of neurons, but its central role is involved in homeostasis. The arcuate nucleus provides many physiological roles involved in feeding, metabolism, fertility, and cardiovascular regulation.
The Adephaga are a suborder of beetles, and with more than 40,000 recorded species in 10 families, the second-largest of the four beetle suborders. Members of this suborder are collectively known as adephagans. The largest family is Carabidae which comprises most of the suborder with over 40,000 species. Adephaga also includes a variety of aquatic beetles, such as predaceous diving beetles and whirligig beetles.
Anti-predator adaptations are mechanisms developed through evolution that assist prey organisms in their constant struggle against predators. Throughout the animal kingdom, adaptations have evolved for every stage of this struggle, namely by avoiding detection, warding off attack, fighting back, or escaping when caught.
A rhinophore is one of a pair of chemosensory club-shaped, rod-shaped or ear-like structures which are the most prominent part of the external head anatomy in sea slugs, marine gastropod opisthobranch mollusks such as the nudibranchs, sea hares (Aplysiomorpha), and sap-sucking sea slugs (Sacoglossa).
Dolabrifera dolabrifera is a species of sea hare, a marine gastropod mollusc in the family Aplysiidae, the sea hares. Dolabrifera dolabrifera, otherwise known as a Warty Seacat. The animal goes by many names, including the common sea hare. The Hawaiian name for Dolabrifera dolabrifera, is Kualakai.
Cephalopod ink is a dark-coloured or luminous ink released into water by most species of cephalopod, usually as an escape mechanism. All cephalopods, with the exception of the Nautilidae and the Cirrina, are able to release ink to confuse predators.
The spotted sea hare is a species of sea slug in the family Aplysiidae, the sea hares. It reaches a length of up to 20 cm (7.9 in) and is found in the northeast Atlantic, ranging from Greenland and Norway to the Mediterranean Sea.
Aplysia vaccaria, also known as the black sea hare and California black sea hare, is a species of extremely large sea slug, a marine, opisthobranch, gastropod mollusk in the family Aplysiidae. It is the largest sea slug species.
Chemical defense is a strategy employed by many organisms to avoid consumption by producing toxic or repellent metabolites or chemical warnings which incite defensive behavioral changes. The production of defensive chemicals occurs in plants, fungi, and bacteria, as well as invertebrate and vertebrate animals. The class of chemicals produced by organisms that are considered defensive may be considered in a strict sense to only apply to those aiding an organism in escaping herbivory or predation. However, the distinction between types of chemical interaction is subjective and defensive chemicals may also be considered to protect against reduced fitness by pests, parasites, and competitors. Repellent rather than toxic metabolites are allomones, a sub category signaling metabolites known as semiochemicals. Many chemicals used for defensive purposes are secondary metabolites derived from primary metabolites which serve a physiological purpose in the organism. Secondary metabolites produced by plants are consumed and sequestered by a variety of arthropods and, in turn, toxins found in some amphibians, snakes, and even birds can be traced back to arthropod prey. There are a variety of special cases for considering mammalian antipredatory adaptations as chemical defenses as well.
Pain in invertebrates is a contentious issue. Although there are numerous definitions of pain, almost all involve two key components. First, nociception is required. This is the ability to detect noxious stimuli which evokes a reflex response that moves the entire animal, or the affected part of its body, away from the source of the stimulus. The concept of nociception does not necessarily imply any adverse, subjective feeling; it is a reflex action. The second component is the experience of "pain" itself, or suffering—i.e., the internal, emotional interpretation of the nociceptive experience. Pain is therefore a private, emotional experience. Pain cannot be directly measured in other animals, including other humans; responses to putatively painful stimuli can be measured, but not the experience itself. To address this problem when assessing the capacity of other species to experience pain, argument-by-analogy is used. This is based on the principle that if a non-human animal's responses to stimuli are similar to those of humans, it is likely to have had an analogous experience. It has been argued that if a pin is stuck in a chimpanzee's finger and they rapidly withdraw their hand, then argument-by-analogy implies that like humans, they felt pain. It has been questioned why the inference does not then follow that a cockroach experiences pain when it writhes after being stuck with a pin. This argument-by-analogy approach to the concept of pain in invertebrates has been followed by others.
Sea hares are gastropods without hard shells, using their specialized ink as their main defensive mechanism instead. Their ink has several purposes, most of which have a chemical basis. For one, the ink serves to cloud the predator's vision as well as halt their senses temporarily. In addition, the chemicals in the ink mimic food. Their skin and digestive tract are toxic to predators as well. They are also seen to change their feeding behaviours in response to averse stimuli.
Aplysioviolin is a purple-colored molecule secreted by sea hares of the genera Aplysia and Dolabella to deter predators. Aplysioviolin is a chemodeterrent, serving to dispel predators on olfactory and gustatory levels as well as by temporarily blinding predators with the molecule's dark color. Aplysioviolin is an important component of secreted ink and is strongly implicated in the sea hares' predatory escape mechanism. While the ink mixture as a whole may produce dangerous hydrogen peroxide and is relatively acidic, the aplysioviolin component alone has not been shown to produce human toxicity.
Aplysia gigantea is a species of sea slug, a shell-less marine gastropod mollusk in the family Aplysiidae. The species was first described in the Journal of the Malacological Society of Australia in 1869. A. gigantea is also known more commonly as the sea hare due to their posterior chemosensory tentacles resembling a hare's ear. A. gigantea is the largest known species in Australia of the opisthobranch genus. The species is known to have toxic effects on terrestrial organisms, particularly domestic dogs. Exposure to this species with dogs has been associated with the development of neurotoxicosis, with symptoms ranging from respiratory distress to tremors, muscle fasciculations, and seizures.