Phenylacetaldoxime dehydratase

Phenylacetaldoxime dehydratase
Identifiers
EC no.	4.99.1.7
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

In enzymology, a phenylacetaldoxime dehydratase (EC 4.99.1.7) is an enzyme that catalyzes the chemical reaction

(Z)-phenylacetaldehyde oxime ${\displaystyle \rightleftharpoons }$ phenylacetonitrile + H₂O

Hence, this enzyme has one substrate, (Z)-phenylacetaldehyde oxime, and two products, phenylacetonitrile and H₂O.

This enzyme belongs to the family of lyases, specifically the "catch-all" class of lyases that do not fit into any other sub-class. The systematic name of this enzyme class is (Z)-phenylacetaldehyde-oxime hydro-lyase (phenylacetonitrile-forming). Other names in common use include PAOx dehydratase, arylacetaldoxime dehydratase, OxdB, and (Z)-phenylacetaldehyde-oxime hydro-lyase. This enzyme participates in styrene degradation.

References

• Kato Y, Nakamura K, Sakiyama H, Mayhew SG, Asano Y (2000). "Novel heme-containing lyase, phenylacetaldoxime dehydratase from Bacillus sp. strain OxB-1: purification, characterization, and molecular cloning of the gene". Biochemistry. 39 (4): 800–9. doi:10.1021/bi991598u. PMID   10651646.
• Kobayashi K, Yoshioka S, Kato Y, Asano Y, Aono S (2005). "Regulation of aldoxime dehydratase activity by redox-dependent change in the coordination structure of the aldoxime-heme complex". J. Biol. Chem. 280 (7): 5486–90. doi: 10.1074/jbc.M410474200 . PMID   15596434.