SUB1

SUB1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1PCF, 2C62, 2PHE, 4USG
Identifiers
Aliases	SUB1 , P15, PC4, p14, SUB1 homolog, transcriptional regulator, SUB1 regulator of transcription
External IDs	OMIM: 600503; MGI: 104811; HomoloGene: 38218; GeneCards: SUB1; OMA:SUB1 - orthologs
Gene location (Human) Chr. Chromosome 5 (human) [1] Band 5p13.3 Start 32,531,633 bp [1] End 32,604,079 bp [1]
Gene location (Mouse) Chr. Chromosome 15 (mouse) [2] Band 15|15 A1 Start 11,981,425 bp [2] End 11,997,069 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in epithelium of nasopharynx lactiferous duct epithelium of colon ganglionic eminence gingival epithelium parotid gland bone marrow cells germinal epithelium endometrium ventricular zone Top expressed in genital tubercle tail of embryo yolk sac abdominal wall primitive streak neural tube gastrula medial ganglionic eminence migratory enteric neural crest cell embryo More reference expression data BioGPS More reference expression data
Gene ontology Molecular function single-stranded DNA binding DNA binding transcription coactivator activity DNA-binding transcription activator activity, RNA polymerase II-specific protein binding RNA binding identical protein binding sequence-specific double-stranded DNA binding Cellular component nucleolus extracellular exosome transcription regulator complex nucleus Biological process regulation of transcription by RNA polymerase II regulation of transcription, DNA-templated transcription by RNA polymerase II SMAD protein signal transduction transcription, DNA-templated positive regulation of transcription initiation from RNA polymerase II promoter Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10923 20024 Ensembl ENSG00000113387 ENSMUSG00000022205 UniProt P53999 P11031 RefSeq (mRNA) NM_006713 NM_011294 RefSeq (protein) NP_006704 NP_035424 Location (UCSC) Chr 5: 32.53 – 32.6 Mb Chr 15: 11.98 – 12 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Activated RNA polymerase II transcriptional coactivator p15 also known as positive cofactor 4 (PC4) or SUB1 homolog is a protein that in humans is encoded by the SUB1 gene. ^{[5] [6] [7]} The human SUB1 gene ^[7] is named after an orthologous gene in yeast. ^[8]

SUB1 is induced by oxidative stress, and is involved in coordinating cellular responses to DNA strand breaks that arise after oxidative stress.Yu L, Ma H, Ji X, Volkert MR (January 2016). "The Sub1 nuclear protein protects DNA from oxidative damage". Molecular and Cellular Biochemistry. 412 (1–2): 165–171. doi:10.1007/s11010-015-2621-x. PMC   5064834 . PMID   26708217. Yeast SUB1 has structural and functional similarities to human alpha-synuclein, a protein that has an important role in Parkinson's disease.Schaser AJ, Osterberg VR, Dent SE, Stackhouse TL, Wakeham CM, Boutros SW, et al. (July 2019). "Alpha-synuclein is a DNA binding protein that modulates DNA repair with implications for Lewy body disorders". Scientific Reports. 9 (1): 10919. doi:10.1038/s41598-019-47227-z. PMC   6662836 . PMID   31358782. Like SUB1, alpha-synuclein functions in regulating DNA repair including repair of DNA double-strand breaks.

Interactions

SUB1 has been shown to interact with CSTF2. ^[9]

References

1. GRCh38: Ensembl release 89: ENSG00000113387 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000022205 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Kretzschmar M, Kaiser K, Lottspeich F, Meisterernst M (August 1994). "A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators". Cell. 78 (3): 525–534. doi:10.1016/0092-8674(94)90429-4. PMID   8062392. S2CID   29077941.
6. Ge H, Roeder RG (August 1994). "Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes". Cell. 78 (3): 513–523. doi:10.1016/0092-8674(94)90428-6. PMID   8062391. S2CID   1140379.
7. "Entrez Gene SUB1: SUB1 homolog (S. cerevisiae)".
8. Knaus R, Pollock R, Guarente L (April 1996). "Yeast SUB1 is a suppressor of TFIIB mutations and has homology to the human co-activator PC4". The EMBO Journal. 15 (8): 1933–1940. doi:10.1002/j.1460-2075.1996.tb00544.x. PMC   450112 . PMID   8617240.
9. Calvo O, Manley JL (May 2001). "Evolutionarily conserved interaction between CstF-64 and PC4 links transcription, polyadenylation, and termination". Molecular Cell. 7 (5): 1013–1023. doi: 10.1016/S1097-2765(01)00236-2 . PMID   11389848.

Further reading

• Kaiser K, Stelzer G, Meisterernst M (July 1995). "The coactivator p15 (PC4) initiates transcriptional activation during TFIIA-TFIID-promoter complex formation". The EMBO Journal. 14 (14): 3520–3527. doi:10.1002/j.1460-2075.1995.tb07358.x. PMC   394420 . PMID   7628453.
• Ge H, Zhao Y, Chait BT, Roeder RG (December 1994). "Phosphorylation negatively regulates the function of coactivator PC4". Proceedings of the National Academy of Sciences of the United States of America. 91 (26): 12691–12695. doi: 10.1073/pnas.91.26.12691 . PMC   45505 . PMID   7809103.
• Brandsen J, Werten S, van der Vliet PC, Meisterernst M, Kroon J, Gros P (November 1997). "C-terminal domain of transcription cofactor PC4 reveals dimeric ssDNA binding site". Nature Structural Biology. 4 (11): 900–903. doi:10.1038/nsb1197-900. PMID   9360603. S2CID   30710386.
• Malik S, Guermah M, Roeder RG (March 1998). "A dynamic model for PC4 coactivator function in RNA polymerase II transcription". Proceedings of the National Academy of Sciences of the United States of America. 95 (5): 2192–2197. Bibcode:1998PNAS...95.2192M. doi: 10.1073/pnas.95.5.2192 . PMC   19292 . PMID   9482861.
• Luo Y, Ge H, Stevens S, Xiao H, Roeder RG (July 1998). "Coactivation by OCA-B: definition of critical regions and synergism with general cofactors". Molecular and Cellular Biology. 18 (7): 3803–3810. doi:10.1128/mcb.18.7.3803. PMC   108964 . PMID   9632764.
• Currie RA (July 1998). "Biochemical characterization of the NF-Y transcription factor complex during B lymphocyte development". The Journal of Biological Chemistry. 273 (29): 18220–18229. doi: 10.1074/jbc.273.29.18220 . PMID   9660784.
• Wang Z, Roeder RG (April 1998). "DNA topoisomerase I and PC4 can interact with human TFIIIC to promote both accurate termination and transcription reinitiation by RNA polymerase III". Molecular Cell. 1 (5): 749–757. doi: 10.1016/S1097-2765(00)80074-X . PMID   9660958.
• Holloway AF, Occhiodoro F, Mittler G, Meisterernst M, Shannon MF (July 2000). "Functional interaction between the HIV transactivator Tat and the transcriptional coactivator PC4 in T cells". The Journal of Biological Chemistry. 275 (28): 21668–21677. doi: 10.1074/jbc.M909058199 . PMID   10887206.
• Yuan CX, Gurley WB (July 2000). "Potential targets for HSF1 within the preinitiation complex". Cell Stress & Chaperones. 5 (3): 229–242. PMC   312889 . PMID   11005381.
• Kumar BR, Swaminathan V, Banerjee S, Kundu TK (May 2001). "p300-mediated acetylation of human transcriptional coactivator PC4 is inhibited by phosphorylation". The Journal of Biological Chemistry. 276 (20): 16804–16809. doi: 10.1074/jbc.M100934200 . PMID   11279157.
• Yu P, Huang B, Shen M, Lau C, Chan E, Michel J, et al. (January 2001). "p15(PAF), a novel PCNA associated factor with increased expression in tumor tissues". Oncogene. 20 (4): 484–489. doi:10.1038/sj.onc.1204113. PMID   11313979. S2CID   39144360.
• Calvo O, Manley JL (May 2001). "Evolutionarily conserved interaction between CstF-64 and PC4 links transcription, polyadenylation, and termination". Molecular Cell. 7 (5): 1013–1023. doi: 10.1016/S1097-2765(01)00236-2 . PMID   11389848.
• Guo H, Cai CQ, Kuo PC (February 2002). "Hepatocyte nuclear factor-4alpha mediates redox sensitivity of inducible nitric-oxide synthase gene transcription". The Journal of Biological Chemistry. 277 (7): 5054–5060. doi: 10.1074/jbc.M109017200 . PMID   11741883. (Retracted, see doi:10.1074/jbc.A116.109017, PMID   28108615,  Retraction Watch . If this is an intentional citation to a retracted paper, please replace {{ retracted |...}} with {{ retracted |...|intentional=yes}}.)
• Dubois T, Zemlickova E, Howell S, Aitken A (February 2003). "Centaurin-alpha 1 associates in vitro and in vivo with nucleolin". Biochemical and Biophysical Research Communications. 301 (2): 502–508. doi:10.1016/S0006-291X(02)03010-3. PMID   12565890.
• Fukuda A, Tokonabe S, Hamada M, Matsumoto M, Tsukui T, Nogi Y, Hisatake K (April 2003). "Alleviation of PC4-mediated transcriptional repression by the ERCC3 helicase activity of general transcription factor TFIIH". The Journal of Biological Chemistry. 278 (17): 14827–14831. doi: 10.1074/jbc.M213172200 . PMID   12590132.
• Swietlicki E, Iordanov H, Fritsch C, Yi L, Levin MS, Rubin DC (2003). "Growth factor regulation of PC4/TIS7, an immediate early gene expressed during gut adaptation after resection". Journal of Parenteral and Enteral Nutrition. 27 (2): 123–131. doi:10.1177/0148607103027002123. PMID   12665168.
• Banerjee S, Kumar BR, Kundu TK (March 2004). "General transcriptional coactivator PC4 activates p53 function". Molecular and Cellular Biology. 24 (5): 2052–2062. doi:10.1128/MCB.24.5.2052-2062.2004. PMC   350566 . PMID   14966284.
• Caldwell RB, Braselmann H, Schoetz U, Heuer S, Scherthan H, Zitzelsberger H (July 2016). "Positive Cofactor 4 (PC4) is critical for DNA repair pathway re-routing in DT40 cells". Scientific Reports. 6: 28890. Bibcode:2016NatSR...628890C. doi:10.1038/srep28890. PMC   4931448 . PMID   27374870.

External links

• Overview of all the structural information available in the PDB for UniProt : P53999 (Activated RNA polymerase II transcriptional coactivator p15) at the PDBe-KB .