SUPT7L

SUPT7L
Identifiers
Aliases	SUPT7L , SPT7L, STAF65, STAF65(gamma), STAF65G, SUPT7H, SPT7-like STAGA complex gamma subunit, SPT7 like, STAGA complex gamma subunit, SPT7 like, STAGA complex subunit gamma
External IDs	OMIM: 612762 MGI: 1919445 HomoloGene: 8907 GeneCards: SUPT7L
Gene location (Human) Chr. Chromosome 2 (human) [1] Band 2p23.3 Start 27,650,809 bp [1] End 27,663,840 bp [1]
Gene location (Mouse) Chr. Chromosome 5 (mouse) [2] Band 5|5 B1 Start 31,671,908 bp [2] End 31,685,151 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in middle temporal gyrus right uterine tube anterior pituitary right lobe of thyroid gland left lobe of thyroid gland Left adrenal gland left coronary artery Brodmann area 9 Brodmann area 23 ascending aorta Top expressed in secondary oocyte aortic valve cumulus cell ascending aorta proximal tubule medial ganglionic eminence lip morula triceps brachii muscle vastus lateralis muscle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transcription coactivator activity protein binding histone acetyltransferase activity protein heterodimerization activity Cellular component nucleus nucleoplasm Biological process regulation of transcription, DNA-templated maintenance of protein location in nucleus histone H3 acetylation transcription, DNA-templated positive regulation of nucleic acid-templated transcription Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9913 72195 Ensembl ENSG00000119760 ENSMUSG00000053134 UniProt O94864 Q9CZV5 RefSeq (mRNA) NM_001282729 NM_001282730 NM_001282731 NM_001282732 NM_014860 NM_028150 RefSeq (protein) NP_001269658 NP_001269659 NP_001269660 NP_001269661 NP_055675 NP_082426 Location (UCSC) Chr 2: 27.65 – 27.66 Mb Chr 5: 31.67 – 31.69 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

STAGA complex 65 subunit gamma is a protein that in humans is encoded by the SUPT7L gene. ^{[5] [6] [7]}

Model organisms

Supt7l knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Abnormal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal [8]
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Eye Histopathology	Normal
Salmonella infection	Normal [9]
Citrobacter infection	Normal [10]
All tests and analysis from [11] [12]

Model organisms have been used in the study of SUPT7L function. A conditional knockout mouse line, called Supt7l^{tm1a(EUCOMM)Wtsi [13] [14]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. ^{[15] [16] [17]}

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. ^{[11] [18]} Twenty four tests were carried out on mutant mice and two significant abnormalities were observed. ^[11] No homozygous mutant embryos were identified during gestation, and therefore none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice but no further abnormalities were observed. ^[11]

Interactions

SUPT7L has been shown to interact with TAF9 ^[6] and Transcription initiation protein SPT3 homolog. ^[6]

References

1. GRCh38: Ensembl release 89: ENSG00000119760 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000053134 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nagase T, Ishikawa K, Suyama M, Kikuno R, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (October 1998). "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 5 (5): 277–86. doi: 10.1093/dnares/5.5.277 . PMID   9872452.
6. Martinez E, Palhan VB, Tjernberg A, Lymar ES, Gamper AM, Kundu TK, Chait BT, Roeder RG (October 2001). "Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo". Molecular and Cellular Biology. 21 (20): 6782–95. doi:10.1128/MCB.21.20.6782-6795.2001. PMC   99856 . PMID   11564863.
7. "Entrez Gene: SUPT7L suppressor of Ty 7 (S. cerevisiae)-like".
8. "Dysmorphology data for Supt7l". Wellcome Trust Sanger Institute.
9. "Salmonella infection data for Supt7l". Wellcome Trust Sanger Institute.
10. "Citrobacter infection data for Supt7l". Wellcome Trust Sanger Institute.
11. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID   85911512.
12. Mouse Resources Portal, Wellcome Trust Sanger Institute.
13. "International Knockout Mouse Consortium".
14. "Mouse Genome Informatics".
15. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (June 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC   3572410 . PMID   21677750.
16. Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi: 10.1038/474262a . PMID   21677718.
17. Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi: 10.1016/j.cell.2006.12.018 . PMID   17218247. S2CID   18872015.
18. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC   3218837 . PMID   21722353.

Further reading

• Nishizaka S, Gomi S, Harada K, Oizumi K, Itoh K, Shichijo S (September 2000). "A new tumor-rejection antigen recognized by cytotoxic T lymphocytes infiltrating into a lung adenocarcinoma". Cancer Research. 60 (17): 4830–7. PMID   10987294.
• Gocke CB, Yu H, Kang J (February 2005). "Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates". The Journal of Biological Chemistry. 280 (6): 5004–12. doi: 10.1074/jbc.M411718200 . PMID   15561718.
• Wang HY, Peng G, Guo Z, Shevach EM, Wang RF (March 2005). "Recognition of a new ARTC1 peptide ligand uniquely expressed in tumor cells by antigen-specific CD4+ regulatory T cells". Journal of Immunology. 174 (5): 2661–70. doi: 10.4049/jimmunol.174.5.2661 . PMID   15728473.
• Soutoglou E, Demény MA, Scheer E, Fienga G, Sassone-Corsi P, Tora L (May 2005). "The nuclear import of TAF10 is regulated by one of its three histone fold domain-containing interaction partners". Molecular and Cellular Biology. 25 (10): 4092–104. doi:10.1128/MCB.25.10.4092-4104.2005. PMC   1087738 . PMID   15870280.