Vertebrate mitochondrial code

Last updated

The vertebrate mitochondrial code (translation table 2) is the genetic code found in the mitochondria of all vertebrata.

Contents

Evolution

AGA and AGG were thought to have become mitochondrial stop codons early in vertebrate evolution. [1] However, at least in humans it has now been shown that AGA and AGG sequences are not recognized as termination codons. A -1 mitoribosome frameshift occurs at the AGA and AGG codons predicted to terminate the CO1 and ND6 open reading frames (ORFs), and consequently both ORFs terminate in the standard UAG codon. [2]

Incomplete stop codons

Mitochondrial genes in some vertebrates (including humans) have incomplete stop codons ending in U or UA, which become complete termination codons (UAA) upon subsequent polyadenylation. [3] [4] [5] [6]

Translation table

Amino acids biochemical propertiesnonpolarpolarbasicacidicTermination: stop codon
Mitochondrial genetic code
1st
base
2nd base3rd
base
UCAG
UUUU(Phe/F) Phenylalanine UCU(Ser/S) Serine UAU(Tyr/Y) Tyrosine UGU(Cys/C) Cysteine U
UUCUCCUACUGCC
UUA(Leu/L) Leucine UCAUAA Stop UGA(Trp/W) Tryptophan     A
UUGUCGUAGUGGG
CCUUCCU(Pro/P) Proline CAU(His/H) Histidine CGU(Arg/R) Arginine U
CUCCCCCACCGCC
CUACCACAA(Gln/Q) Glutamine CGAA
CUGCCGCAGCGGG
AAUU(Ile/I) Isoleucine ACU(Thr/T) Threonine         AAU(Asn/N) Asparagine AGU(Ser/S) Serine U
AUCACCAACAGCC
AUA(Met/M) Methionine ACAAAA(Lys/K) Lysine AGA Stop A
AUG [A] ACGAAGAGGG
GGUU(Val/V) Valine GCU(Ala/A) Alanine GAU(Asp/D) Aspartic acid GGU(Gly/G) Glycine U
GUCGCCGACGGCC
GUAGCAGAA(Glu/E) Glutamic acid GGAA
GUGGCGGAGGGGG
A The codon AUG both codes for methionine and serves as an initiation site: the first AUG in an mRNA's coding region is where translation into protein begins. [7]

Differences from the standard code

DNA codonsRNA codonsThis code (2) Standard code (1)
AGAAGASTOP = Ter(*)Arg(R)
AGGAGGSTOP = Ter(*)Arg(R)
ATAAUAMet(M)Ile(I)
TGAUGATrp(W)STOP = Ter(*)

Alternative initiation codons

See also

References

  1. S. Osawa, T. Ohama, T. H. Jukes and K. Watanabe (September 1989). "Evolution of the mitochondrial genetic code. I. Origin of AGR serine and stop codons in metazoan mitochondria". J Mol Evol. 29 (3): 202–7. Bibcode:1989JMolE..29..202O. doi:10.1007/bf02100203. PMID   2506356. S2CID   21051869.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  2. R J Temperley; R Richter; S Dennerlein; R N Lightowlers; Z M Chrzanowska-Lightowlers (January 2010). "Hungry codons promote frameshifting in human mitochondrial ribosomes". Science. 327 (5963): 301. Bibcode:2010Sci...327..301T. doi:10.1126/science.1180674. PMID   20075246. S2CID   206522983.
  3. Temperley, R. J.; Wydro, M; Lightowlers, R. N.; Chrzanowska-Lightowlers, Z. M. (2010). "Human mitochondrial mRNAs--like members of all families, similar but different". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1797 (6–7): 1081–5. doi:10.1016/j.bbabio.2010.02.036. PMC   3003153 . PMID   20211597.
  4. W. R. Hou, Y. Chen, X. Wu, J. C. Hu, Z. S. Peng, J. Yang, Z. X. Tang, C. Q. Zhou, Y. M. Li, S. K. Yang, Y. J. Du, L. L. Kong, Z. L. Ren, H. Y. Zhang and S. S. Shuai (December 2006). "A complete mitochondrial genome sequence of Asian black bear Sichuan subspecies (Ursus thibetanus mupinensis)". Int J Biol Sci. 3 (2): 85–90. doi:10.7150/ijbs.3.85. PMC   1752227 . PMID   17205108.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  5. Oh, D. J.; Kim, J. Y.; Lee, J. A.; Yoon, W. J.; Park, S. Y.; Jung, Y. H. (2007). "Complete mitochondrial genome of the rabbitfish Siganus fuscescens (Perciformes, Siganidae)". DNA Sequence. 18 (4): 295–301. doi:10.1080/10425170701248525. PMID   17541835. S2CID   38458668.
  6. Ki, J. S.; Hwang, D. S.; Park, T. J.; Han, S. H.; Lee, J. S. (2009). "A comparative analysis of the complete mitochondrial genome of the Eurasian otter Lutra lutra (Carnivora; Mustelidae)". Molecular Biology Reports. 37 (4): 1943–55. doi:10.1007/s11033-009-9641-0. PMID   19757186. S2CID   24233602.
  7. Nakamoto T (March 2009). "Evolution and the universality of the mechanism of initiation of protein synthesis". Gene. 432 (1–2): 1–6. doi:10.1016/j.gene.2008.11.001. PMID   19056476.
  8. P. Desjardins & R. Morais (February 1991). "Nucleotide sequence and evolution of coding and noncoding regions of a quail mitochondrial genome". J Mol Evol. 32 (2): 153–161. Bibcode:1991JMolE..32..153D. doi:10.1007/bf02515387. PMID   1706782. S2CID   1974138.
  9. The Genetic Codes